• Background/Aim: To demonstrate the value of Ki-67 in distinguishing between partial and complete hydatidiform moles. (iiarjournals.org)
  • We aimed to assess the prognostic value of Ki-67 index after neoadjuvant endocrine therapy (NET). (bvsalud.org)
  • Antigen KI-67 also known as Ki-67 or MKI67 is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). (journalcra.com)
  • Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
  • The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
  • The following product was used in this experiment: Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 615, eBioscience™ from Thermo Fisher Scientific, catalog # 366-5698-82, RRID AB_2925428. (thermofisher.com)
  • Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet 615. (thermofisher.com)
  • Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. (thermofisher.com)
  • The following product was used in this experiment: Ki67 Monoclonal Antibody (OTI5D7), TrueMAB™ from Thermo Fisher Scientific, catalog # TA801577. (thermofisher.com)
  • The antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. (journalcra.com)
  • Antigen KI-67 is a nuclear protein that is associated with cellular proliferation and ribosomal RNA transcription. (wikipedia.org)
  • The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. (wikipedia.org)
  • During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. (wikipedia.org)
  • Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
  • Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. (wikipedia.org)
  • Ki-67 (protein) has been shown to interact with CBX3. (wikipedia.org)
  • Protein Ki-67 in human MCF-7 cells Ki-67 protein (red), tubulin (green) and DNA (blue) in HeLa cells. (wikipedia.org)
  • Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). (thermofisher.com)
  • Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. (thermofisher.com)
  • Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. (biolegend.com)
  • Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. (biolegend.com)
  • E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa. (biolegend.com)
  • Predictive Role of p53 Protein as a Single Marker or Associated to Ki67 Antigen in Oral Carcinogenesis. (bvsalud.org)
  • Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis, but does not significantly affect cell proliferation in vivo: Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
  • Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. (thermofisher.com)
  • Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation. (thermofisher.com)
  • Ki-67 is a biomarker commonly used for evaluating the proliferation of cancer cells in breast cancer patients. (bvsalud.org)
  • Ki-67 is detected within the nucleus during interphase but redistributes to the chromosomes during mitosis. (thermofisher.com)
  • Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. (thermofisher.com)
  • Ki-67 is an excellent marker to determine the growth fraction of a given cell population, the fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (journalcra.com)
  • Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
  • Ki-67 is used as a marker for determining the growth fraction of a given population of cells. (thermofisher.com)
  • Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors. (biolegend.com)
  • The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
  • There is tumor cell positivity in 70% of the cells: Ki-67 labelling index = 70% Counting positive versus negative nuclei with Ki-67 labeling, in this case in a neuroendocrine tumor of the small intestine. (wikipedia.org)
  • In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. (thermofisher.com)
  • CONCLUSIONS: Negative p16INK4a and Ki67 immunohistochemical staining can assure absence of a higher-grade lesion in more than 85% of patients with CIN2 biopsies and can be used to prevent overtreatment of these patients. (bvsalud.org)
  • OBJECTIVES: To determine the ability of p16INK4a and Ki-67 staining in biopsies diagnosed with CIN2 to identify patients with higher-grade lesions (CIN3 or carcinoma). (bvsalud.org)
  • 9. Iamaroon A, Khemaleelakul U, Pongsiriwet S, Pintong J. Co-expression of p53 and Ki67 and lack of EBV expression in oral squamous cell carcinoma. (bvsalud.org)
  • Expression of p53 in leukoplakia and squamous cell carcinoma of the oral mucosa: correlation with expression of Ki67. (bvsalud.org)
  • High Ki-67 expression in an invasive breast cancer, with cancer nuclei being stained (brown). (wikipedia.org)
  • Conclusion: The Ki-67 expression was higher in complete than in partial hydatidiform moles. (iiarjournals.org)
  • METHODS: In 79 women, we analyzed the correlation of p16INK4a and Ki-67 expression in CIN2 biopsies with the presence of a higher-grade lesions, as determined via histopathology in surgical specimens from treated women or via two colposcopies and two cytological tests during follow-up for untreated women with at least a 6-month interval. (bvsalud.org)
  • Ki-67 expression positively and negatively predicted the presence of higher-grade lesions in 40% and 88.24% patients, respectively. (bvsalud.org)
  • The correlation between low Ki-67 index and histologically low-grade tumors is strong. (thermofisher.com)
  • Results: From the 121 recruited women, 76 screened positive in at least one exam (LBCC or DS-p16/Ki-67) and had a diagnostic conclusion. (edu.br)
  • Objective: To access p16/Ki-67 double staining (DS) in cervical cells performance for detecting precursor lesions of cervical cancer, in comparison to immediate repetition of the liquid-based cervical cytology (LBCC) in women with screening cytology results of atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesion (LSIL). (edu.br)
  • DS-p16/Ki-67 identified a greater number of women with CIN2-3, addressing 50% of the women to colposcopy, but failing to detect these lesions in 50% of cases. (edu.br)
  • Is Ki-67 of Diagnostic Value in Distinguishing Between Partial and Complete Hydatidiform Moles? (iiarjournals.org)
  • Positive IHC staining for p16INK4a and Ki-67 did not predict CIN3 in patients with CIN2 biopsies. (bvsalud.org)
  • METHODS: We conducted a systematic literature search of PubMed, Embase, CENTRAL, and conference proceedings up to 22nd August 2023 to identify studies reporting the association of Ki-67 index after NET with recurrence-free survival (RFS) and/or overall survival (OS) in women with ER-positive/HER2-negative early breast cancer. (bvsalud.org)
  • Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component of microtubules. (wikipedia.org)
  • The images were uploaded to the DeepLiif software to detect the exact percentage of Ki-67 positive cells. (bvsalud.org)
  • MIB-1 is used in clinical applications to determine the Ki-67 labelling index. (wikipedia.org)
  • An expert pathologist (P1) evaluated the Ki-67 index of the hotspot fields using the eyeball method. (bvsalud.org)
  • Objective: To compare the automated detection of Ki-67 with the manual eyeball/hotspot method. (bvsalud.org)
  • One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
  • Results: Positive immmuno-staining for Ki67 was identified in 88% of the patients. (journalcra.com)
  • Immunofluorescent antibody staining against neurofilament (green) and Ki-67 (red) in a mouse embryo 12.5 days after fertilization. (wikipedia.org)
  • In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy. (wikipedia.org)
  • In this study, we used a novel deep learning model to quantify Ki-67 in breast cancer in digital images prepared by a microscope-attached camera. (bvsalud.org)
  • The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. (thermofisher.com)
  • Women were addressed to the Cervical Referral Services (SRC) in each state and examined with colposcopy, LBCC and DS-p16/Ki-67. (edu.br)