ImmunogenImmunogenicPeptideAntibodyReactivityProteinTumor-associatedAntibodiesAntigensImmunizationSyntheticMiceELISAProteinsBoundCellsImmunePeptidesReactivityPolyclonalVaccineOvalbuminAntigenCrenulataVaccinesBovine Serum AFlow cytometrySyntheticImmunologicalMolecular weightVaccinationClonesInduceMoleculesSpecificMouseTypeFound
Immunogen1
- Using this strategy, four peptides (TK 1-4) were selected and used as immunogen in mice in the form of either keyhole limpet hemocyanin (KLH) conjugates or peptide-encoding minigenes. (unime.it)
Immunogenic3
- Aggregates remain immunogenic, but limit the ability to conjugate haptens, and are difficult to manipulate in the laboratory. (wikipedia.org)
- Immunization with tumor TCR protein conjugated to the immunogenic protein keyhole limpet hemocyanin (KLH) protects mice from tumor challenge with the murine T cell lymphoma C6VL. (elsevierpure.com)
- Strategies, which have been designed to overcome this lack of immunogenicity in infants, include the linking of the polysaccharide to large immunogenic proteins, which provide bystander T-cell help and which induce immunological memory against the polysaccharide antigen to which it is conjugated. (allindianpatents.com)
Peptide7
- Synthetic peptide corresponding to Mouse CDKN2A/p19ARF conjugated to keyhole limpet haemocyanin. (abcam.com)
- Rindopepimut (also known as CDX-110), a vaccine targeting the EGFR deletion mutation EGFRvIII, consists of an EGFRvIII-specific peptide conjugated to keyhole limpet haemocyanin. (nih.gov)
- Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. (antibodiesinc.com)
- Synthetic peptide corresponding to Human Apolipoprotein E4 conjugated to Keyhole Limpet Haemocyanin (KLH). (creative-biolabs.com)
- Synthetic peptide corresponding to Human Histone H3 aa 23-35 (phospho S28) conjugated to Keyhole Limpet Haemocyanin (KLH). (abcam.co.jp)
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH). (aveslabs.com)
- Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat gephyrin, conjugated to keyhole limpet hemocyanin (KLH). (phosphosolutions.com)
Antibody6
- This method is the simplest to perform and the "random" orientation allows for antibody generation against all possible epitopes, but it generally results in some degree of polymerization, which decreases solubility making the conjugate more difficult to handle. (wikipedia.org)
- Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human c-myc antibody ( MCA2200 ) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence. (bio-rad-antibodies.com)
- Alexa-FluorA647 conjugated Mouse anti Human c-myc antibody, clone 9E10 ( MCA2200A647 ) used for the evaluation of c-myc on dicidual NK cells demonstrating the correlation between c-myc and CD122 expression. (bio-rad-antibodies.com)
- The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. (bdbiosciences.com)
- The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. (bdbiosciences.com)
- The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. (bdbiosciences.com)
Reactivity1
- MAbs were further characterized by ELISA and western blot against various monoisocyanate (mNCO) and dNCO and diisothiocyanate conjugated proteins to identify reactivity toward urea, amide or thiourea linkages. (cdc.gov)
Protein4
- There are two keyhole limpet hemocyanin genes, termed KLH1 and KLH2 which share around 60% identity at the protein level. (wikipedia.org)
- Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of antibodies for research, biotechnology and therapeutic applications. (wikipedia.org)
- mAbs specific for only 2,4-TDI-conjugated protein did not recognize 2,6-TDI-HSA, mNCO-HSA or other dNCO-HSAs. (cdc.gov)
- Other cross-reactive mAbs reacted with 2,4-TDI-HSA, 2,6-TDI-HSA, and other dNCO protein conjugates like methylene diphenyldiisocyanate- and hexamethelene diisocyanate-HSA, but not mNCO-HSA. (cdc.gov)
Tumor-associated1
- These vaccines contain specific tumor-associated antigens conjugated to KLH to stimulate anti-tumor immune responses which can destroy tumor cells. (wikipedia.org)
Antibodies2
- Conjugated primary antibodies with 490 nm, 550 nm, 594 nm, and 650 nm fluorophores! (antibodiesinc.com)
- When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. (bdbiosciences.com)
Antigens1
- Self-adjuvanting vaccines, the conjugates of immunoenhancers and antigens, are anticipated to stimulate antigen-specific immune system responses without extreme inflammation.6C8 Furthermore, they possess well-defined structures and facilitate quality control of vaccine products hence. (lavoixdesrroms.org)
Immunization1
- PR-07) corresponding to the extracellular domain conjugated to keyhole limpet hemocyanin (KLH) for immunization. (atsbio.com)
Synthetic1
- Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH). (scientificlabs.ie)
Mice2
- BALB/c mice were immunized with 2,4- or 2,6-TDI conjugated keyhole limpet hemocyanin (KLH), spleen cells isolated and hybridomas made. (cdc.gov)
- As anticipated31, and mice with 4-hydroxy-3-nitrophenyl-acetyl conjugated to keyhole limpet hemocyanin (NP-KLH). (tech-strategy.org)
ELISA1
- Resultant mAbs produced by these hybridomas were screened for their ability to bind TDI conjugated human serum albumin (HSA) by ELISA. (cdc.gov)
Proteins1
- In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically-based research techniques in mammalian model organisms. (wikipedia.org)
Bound2
- When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color, due to the Cu2+ state of the copper. (wikipedia.org)
- In the absence of oxygen, the bound copper is found as Cu1+ and hemocyanin is colorless. (wikipedia.org)
Cells1
- Wiens, GD, Brown, M & Rittenberg, MB 2003, ' Repertoire shift in the humoral response to phosphocholine-keyhole limpet hemocyanin: V H somatic mutation in germinal center B cells impairs T15 Ig function ', Journal of Immunology , vol. 170, no. 10, pp. 5095-5102. (elsevierpure.com)
Immune1
- In the self-adjuvanting vaccine technique, T-helper (Th) cell epitopes and innate immune system ligands are often conjugated as immunoenhancers. (lavoixdesrroms.org)
Peptides3
- Specific for free dityrosine, 3-(p-hydroxyphenyl) propionic acid dimer, dityrosine-BSA conjugate, and dityrosine in protein or peptides. (caslab.com)
- Chemically synthesized peptides corresponding to those displayed by the 6 phage clones were conjugated to keyhole limpet hemocyanin (KLH) carrier protein and tested for their binding specificity of the respective protective MAbs. (nih.gov)
- The synthesized peptides had been purified by reversed stage high-performance liquid chromatography and crosslinked with keyhole limpet hemocyanin by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.8 Rabbits had been immunized 3 x (100?g/rabbit) using the conjugated peptides. (himafund.org)
Reactivity1
- MAbs were further characterized by ELISA and western blot against various monoisocyanate (mNCO) and dNCO and diisothiocyanate conjugated proteins to identify reactivity toward urea, amide or thiourea linkages. (cdc.gov)
Polyclonal2
- 13. Polyclonal antibodies from patients immunized with a globo H-keyhole limpet hemocyanin vaccine: isolation, quantification, and characterization of immune responses by using totally synthetic immobilized tumor antigens. (nih.gov)
- The sensitivity and specificity from the LM23Ckeyhole limpet hemocyanin-generated rabbit polyclonal antibody were evaluated by European and ELISA blot. (himafund.org)
Vaccine5
- 3. A preclinical study comparing approaches for augmenting the immunogenicity of a heptavalent KLH-conjugate vaccine against epithelial cancers. (nih.gov)
- 6. Consistent antibody response against ganglioside GD2 induced in patients with melanoma by a GD2 lactone-keyhole limpet hemocyanin conjugate vaccine plus immunological adjuvant QS-21. (nih.gov)
- 10. Thomsen-Friedenreich (TF) antigen as a target for prostate cancer vaccine: clinical trial results with TF cluster (c)-KLH plus QS21 conjugate vaccine in patients with biochemically relapsed prostate cancer. (nih.gov)
- 11. Immunogenicity of a fucosyl-GM1-keyhole limpet hemocyanin conjugate vaccine in patients with small cell lung cancer. (nih.gov)
- The candidate vaccine that has been furthest along in development is CYT006-AngQb, a conjugate vaccine composed of modified angiotensin II covalently linked to recombinant virus-like particles derived from the RNA bacteriophage Q-beta. (medscape.com)
Ovalbumin1
- Six rounds of panning were performed against azoxystrobin conjugated to either ovalbumin (OVA) or rabbit serum albumin (RSA) to enrich clones expressing VHH specific to the hapten. (uoguelph.ca)
Antigen2
- Immunizations with TNP and DNP conjugates of SIII or of BA were always by intravenous injection\of antigen in dosages as described in the tables. (nih.gov)
- The B81-3 monoclonal antibody was raised against keyhole limpet hemocyanin (KLH), an antigen not expressed by mammalian cells or cell lines. (bdbiosciences.com)
Crenulata2
- Keyhole limpet hemocyanin (KLH) is a large, multisubunit, oxygen-carrying, metalloprotein that is found in the hemolymph of the giant keyhole limpet, Megathura crenulata, a species of keyhole limpet that lives off the coast of California, from Monterey Bay to Isla Asuncion off Baja California. (wikipedia.org)
- it is available only as a purified biological product from the keyhole limpet Megathura crenulata. (wikipedia.org)
Vaccines5
- These vaccines contain specific tumor-associated antigens conjugated to KLH to stimulate anti-tumor immune responses which can destroy tumor cells. (wikipedia.org)
- 5. Comparison of antibody titers after immunization with monovalent or tetravalent KLH conjugate vaccines. (nih.gov)
- 16. Keyhole limpet hemocyanin conjugate vaccines against cancer: the Memorial Sloan Kettering experience. (nih.gov)
- Morphine conjugate vaccines have effectively reduced behavioral effects of heroin in rodents and primates. (aspetjournals.org)
- These data support a critical role for 6-MAM in mediating the early effects of i.v. heroin and suggest that reducing 6-MAM concentration in brain is essential to the efficacy of morphine conjugate vaccines. (aspetjournals.org)
Bovine Serum A1
- To isolate anti-azoxystrobin V HHs, a hyper-immunized library was constructed by isolating the V HH-coding genes from lymphocytes collected from a llama immunized with azoxystrobin conjugated to bovine serum albumin (BSA). (uoguelph.ca)
Flow cytometry1
- This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates. (bdbiosciences.com)
Synthetic2
- 8. Immunogenicity of synthetic TF-KLH (keyhole limpet hemocyanin) and sTn-KLH conjugates in colorectal carcinoma patients. (nih.gov)
- Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH). (scientificlabs.ie)
Immunological2
- 15. Effect of immunological adjuvant combinations on the antibody and T-cell response to vaccination with MUC1-KLH and GD3-KLH conjugates. (nih.gov)
- The present studies were undertaken to determine (i) whether the trinitro- phenyl-lysine conjugates of SII (TNP-SIII) could induce an immune response to TNP with generation of immunological memory, and (ii) whether or not the inhibition of 7S memory expression observed by Mitchell e¢ al. (nih.gov)
Molecular weight1
- A low molecular weight polymer-conjugated platinum complex with potential antineoplastic activity. (blogspot.com)
Vaccination2
- 7. Vaccination of small cell lung cancer patients with polysialic acid or N-propionylated polysialic acid conjugated to keyhole limpet hemocyanin. (nih.gov)
- Vaccination of high-risk breast cancer patients with mucin-1 (MUC1) keyhole limpet hemocyanin conjugate plus QS-21. (southernbiotech.com)
Clones1
- A total of 376 clones from rounds two and three were screened for their specific binding to monensin conjugates and the positive clones were sequenced. (uoguelph.ca)
Induce1
- When administered to a host alone, as polymers or as carrier-bound conjugates, the polypeptides induce the proliferation of thymus-derived cells in hosts primed against hepatitis B virus. (justia.com)
Molecules1
- On the other hand, hapten conjugates of nonimmunogenic molecules such as a copolymer of p-glutamic acid and p-lysine (p-GL) (6-8), syngeneic y-globulin (9, 10), and syngeneic red cells 1 Supported by Grant AI-3076 from the United States Public Health Service and in part by Grant GFB13592 AI from the National Science Foundation. (nih.gov)
Specific1
- The A112-3 clone is specific for keyhole limpet hemocyanin, (KLH). (bdbiosciences.com)
Mouse1
- The IC50 values of these VHHs were between 2 and 11.3 [mu]M. Anti-monensin scFvs were isolated from another hyperimmunized-phage-displayed library originating from splenocytes of a mouse immunized with monensin conjugated to BSA. (uoguelph.ca)
Type2
- CELLULAR IMMUNOLOGY 15, 321-335 (1975) Induction of Primary and Inhibition of Secondary Antibody Response to Hapten by Hapten Conjugates of Type III Pneumococcal Polysaccharide? (nih.gov)
- Another promising approach to this problem was initiated by Mitchell et a/. (15), who demonstrated interference with the 7S antibody memory response to DNP-hemocyanin through injection of the DNP-lysine conjugates of pneumococcal polysaccharide type III (DNP-SITT). (nih.gov)
Found1
- In the absence of oxygen, the bound copper is found as Cu1+ and hemocyanin is colorless. (wikipedia.org)