• We have characterized an unusual cell phenotype in third passage cultures of a human keratinocyte strain derived from newborn foreskin epidermis. (rupress.org)
  • Watt's major research contribution has been to elucidate how the outer covering of mammalian skin, the epidermis, is maintained through self-renewal of stem cells and terminal differentiation of their progeny. (wikipedia.org)
  • Constitutive activation of MAPK increased the growth rate of human keratinocytes and delayed the onset of terminal differentiation, recreating many of the histological features of psoriatic epidermis. (jci.org)
  • The lentivirus-mediated overexpression of psoriasin, mimicking the psoriatic milieu, gave rise to an altered regulation of differentiation genes and an expression pattern reminiscent of that in psoriatic epidermis. (medicaljournals.se)
  • These findings suggest that psoriasin contributes to the dysregulated differentiation process in the psoriasis epidermis. (medicaljournals.se)
  • In this process, the keratinocytes progress from the basal layer of the epidermis through distinct stages to form the spinous, granular and cornified layers. (medicaljournals.se)
  • In addition to the low expression in normal skin and the high expression in the psoriatic epidermis, psoriasin is also expressed in conditions displaying abnormal differentiation. (medicaljournals.se)
  • In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)
  • The high expression level in psoriatic epidermis in conjunction with the disturbed differentiation process suggests that psoriasin is involved in keratinocyte differentiation. (medicaljournals.se)
  • The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. (thomassci.com)
  • The barrier dysfunction, which may facilitate allergen entry, as a key epidermis also contains keratinocytes and Langerhans cells, a factor in the development of atopic asthma and sensitization. (cdc.gov)
  • The epidermis is a dynamic tissue in which keratinocytes proliferate in the basal layer and undergo a tightly controlled differentiation while moving into the suprabasal layers. (omicsdi.org)
  • We observed activation of mitogen-activated protein kinase (MAPK) in basal and suprabasal keratinocytes of human and transgenic mouse psoriatic lesions and healing mouse skin wounds, correlating in each case with suprabasal integrin expression. (jci.org)
  • This pattern of expression suggests an involvement in epithelial differentiation. (medicaljournals.se)
  • The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. (thomassci.com)
  • The inverse correlation between Notch signaling and the proliferative status of the corneal epithelium is consistent with the idea that Notch plays a role in corneal epithelial differentiation. (molvis.org)
  • HaCaT is an immortalised human adult keratinocyte cell line. (reprocell.com)
  • This protocol describes a method for differentiation of HaCaT cells under air-exposed conditions for up to 21 days. (reprocell.com)
  • It strongly suppressed P.acnes growth, and reduced inflammation by suppressing the TLR-2/NF- kB pathway in SZ-95 sebocytes and HaCaT keratinocytes. (cosmeticsbusiness.com)
  • In this research, we verified the effect of GMP on treatment for acne, including P.acnes suppression, anti-inflammation on human SZ-95 sebocytes and HaCaT keratinocytes, as well as beneficial effects on follicular hyperkeratosis. (cosmeticsbusiness.com)
  • IL-17A and TNF-α, 2 cytokines intimately involved in the development of psoriatic lesions, suppressed galectin-7 expression in human primary keratinocytes (HEKn cells) and the immortalized human keratinocyte cell line HaCaT. (omicsdi.org)
  • To gain a deeper insight into the diversity of keratin functions we have transfected human HaCaT keratinocytes with plasmids coding for several acidic keratins. (exposed-skin-care.net)
  • The Notch signaling pathway plays a key role in the proliferation and differentiation of many tissues. (molvis.org)
  • EGF family members, including AR, induce proliferation and differentiation of typical and malignant epithelial cells, fibroblasts and keratinocytes 1, 7. (m-entrepreneurship.com)
  • Because S1P induces differentiation of keratinocytes, we examined whether modulation of S1P lyase and altered intracellular S1P levels regulate proliferation and differentiation of human neonatal epidermal keratinocyte (HEKn) cells. (omicsdi.org)
  • Collectively, these findings suggest that S1P lyase is a modulating factor for proliferation and differentiation, and support its potential as a therapeutic target for psoriasis in human keratinocytes. (omicsdi.org)
  • they formed desmosomes and expressed the keratin profile characteristic of normal keratinocytes in culture. (rupress.org)
  • After 21 days, cell differentiation is noticeable within the construct, indicated by intense pink staining of the cell cytoplasm due to increased keratin production, anucleation and flattening of cell morphology. (reprocell.com)
  • This is further supported by the expression of late differentiation markers (keratin 10 and involucrin) which occur towards the air exposed surface of the culture with increasing expression from 7-21 days (Figure 3). (reprocell.com)
  • In HEKn cells, pharmacological treatment with the SLI caused G1 arrest by upregulation of p21 and p27 and induced keratin 1, an early differentiation marker. (omicsdi.org)
  • In addition, enzyme suppression by siSGPL1 upregulated keratin 1 and differentiation markers including involucrin and loricrin. (omicsdi.org)
  • Keratinocytes are immature skin cells that produce keratin, a tough protein that helps form hair and nails as well as skin. (ijpsr.com)
  • The members from the large keratin family of cytoskeletal proteins are expressed in a carefully regulated tissue- and differentiation-specific manner. (exposed-skin-care.net)
  • These findings demonstrate that chronic exposure of esophageal keratinocytes to acid pH induces transcription of cdx 2, an intestinal specific homeobox gene known to play a critical role in the differentiation and maintenance of intestinal epithelial functions. (silverchair.com)
  • We thus demonstrated that S1P lyase inhibition reduces cell proliferation and induces keratinocyte differentiation, and that inhibition may attenuate psoriasiform changes. (omicsdi.org)
  • In the nucleus, S1A11 suppresses the growth of keratinocytes through p21 (CIP1/WAF1) activation and induces cell differentiation. (betalifesci.com)
  • This cell line has been known to retain a capacity fornormal differentiation up to multiple passages similar to normal human epidermal keratinocytes (NHEK), and hence offers a suitable model for keratinisation studies. (reprocell.com)
  • The present results suggest that activation of Akt-1 may provide specific populations of epidermal keratinocytes that develop into skin tumors with the ability to resist terminal differentiation and have enhanced proliferation during multi-stage skin carcinogenesis. (uky.edu)
  • Clonal proliferation of atypical keratinocytes showing abnormal terminal keratinocyte differentiation leads to the formation of the cornoid lamella. (medscape.com)
  • [ 1 ] This expands peripherally and forms the raised boundary between abnormal and normal keratinocytes. (medscape.com)
  • The atypical keratinocytes show abnormal differentiation but do not show an increased rate of proliferation. (medscape.com)
  • Increased sebum production under the inducing factors such as diet, living habits and androgens, abnormal keratinisation of keratinocytes, colonization by Propionibacterium acnes (P.acnes) and inflammation are thought to be the major pathogenic factors. (cosmeticsbusiness.com)
  • This abnormal immune response causes rapid proliferation of keratinocytes (immature skin cells) and inflammation. (ijpsr.com)
  • Induction of keratinocyte differentiation caused concurrent expression of psoriasin and the differentiation marker involucrin. (medicaljournals.se)
  • The downregulation of psoriasin expression by small interfering RNA revealed that psoriasin mediates the expression of involucrin, desmoglein 1, transglutaminase 1 and CD24 in normal differentiation. (medicaljournals.se)
  • In 2D culture, this is characterized by lower expression of early (keratins 10 and 13) and late (involucrin and filaggrin) epidermal differentiation markers, better proliferation, elevated clonogenicity and an increased proportion of keratinocyte stem cells. (skinsoc.org.sg)
  • Luteolin-7-glucoside inhibits IL-22/STAT3 pathway, reducing proliferation, acanthosis, and inflammation in keratinocytes and in mouse psoriatic model. (omicsdi.org)
  • Cells were labeled with antibodies to the differentiation markers indicated. (jci.org)
  • The keratinocytes in active psoriatic lesions display an altered sequence and altered expression levels of the differentiation markers (2). (medicaljournals.se)
  • Moreover, in the Imiquimod (IMQ) mouse model of psoriasis, topical administration of LUT-7G leads to a marked reduction of acanthosis and re-expression of epidermal differentiation markers. (omicsdi.org)
  • The complex processes that direct cell-specific differentiation are not completely understood, but once the differentiation program is activated cells usually maintain their specific phenotype. (silverchair.com)
  • Moreover, hEGFR KI/KI mice display accelerated chondrocyte and osteoblast differentiation, a phenotype that is also present in Egfr -/- mice and has not been previously described. (silverchair.com)
  • Previous studies of the role of psoriasin in keratinocyte differentiation have focused on the effects of extracellular psoriasin, but as the psoriasin in psoriasis is keratinocyte-derived, it is imperative to consider the effects of psoriasin as an endogenously produced intracellular mediator. (medicaljournals.se)
  • Lamellar granules are intracellular granules that originate from the Golgi apparatus of keratinocytes in the stratum corneum. (medscape.com)
  • The differentiation-induced psoriasin expression was found to be mediated by the protein kinase C pathway. (medicaljournals.se)
  • Ganglioside GQ1b promotes the differentiation of human neuroblastoma cells, murine embryonic stem cells via the activation of the ERK1/2 pathway, and murine keratinocytes via phosphoinositide turnover. (enzolifesciences.com)
  • Galectin-7 attenuated IL-17A-induced production of inflammatory mediators by keratinocytes via the microRNA-146a/ERK pathway. (omicsdi.org)
  • RNA sequencing revealed the upregulation of inflammasome pathway-related genes, keratinocyte stress marker genes, and keratinocyte differentiation marker genes in the Nlrp1b KI mice after UVB irradiation. (frontiersin.org)
  • The dermis contains nerves and blood and lymphatic vessels, which supply the abnormally multiplying keratinocytes with their blood supply and also transport potent immune factors that cause the underlying inflammation and redness 5 . (ijpsr.com)
  • Regarding photoaging, UVB radiation and oxidative stress induce dose-dependent premature terminal differentiation and senescence of human primary keratinocytes, as well as inflammation. (skinsoc.org.sg)
  • Numerous secreted and cell surface proteins relevant to airway inflammation and remodeling are initially synthesized as inactive precursor proteins, including growth/differentiation factors and their associated receptors, enzymes, adhesion molecules, neuropeptides, and peptide hormones. (molcells.org)
  • The ndk cells also differed from normal keratinocytes in that they did not require a feeder layer and were not stimulated by cholera toxin to proliferate. (rupress.org)
  • Although ndk failed to undergo terminal differentiation in culture, they were not transformed, since they were still sensitive to contact inhibition of growth, did not proliferate in soft agar, and had a limited lifespan in culture. (rupress.org)
  • In psoriasis, however, the keratinocytes proliferate very rapidly and travel from the basal layer to the surface in only about four days. (ijpsr.com)
  • The superficial keratinocytes ultimately are shed with the stratum corneum, so no lytic phase exists in the HPV life cycle, as the apoptotic keratinocytes release the virus during degradation in the mucosal lumen (see the image below). (medscape.com)
  • In normal cell growth, keratinocytes mature and migrate from the bottom (basal) layer to the surface and are shed unobtrusively. (ijpsr.com)
  • In 3D organotypic skin models cultured in standard conditions, NAM inhibits differentiation of the upper epidermal layers while maintaining proliferation at the basal layer. (skinsoc.org.sg)
  • This is supported by reduced proliferation of human dermal papilla and predominantly epithelial keratinocytes after incubation with AICAR, the agonist for AMPK signaling which activates PPARGC1a and serves as co-activator of PPARγ. (nature.com)
  • Inhibition of sphingosine 1-phosphate lyase activates human keratinocyte differentiation and attenuates psoriasis in mice. (omicsdi.org)
  • In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)
  • However, unlike normal keratinocytes, the cells did not grow as compact colonies and did not stratify or undergo terminal differentiation, even after TPA treatment or suspension culture. (rupress.org)
  • We suggest that these cells are a useful experimental adjunct to cultures of normal keratinocytes, in which proliferation and terminal differentiation are tightly coordinated, because in ndk cells there appears to be a block in terminal differentiation. (rupress.org)
  • In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)
  • When hyperproliferation of HEKn cells was induced by interleukin (IL)-17 and IL-22, pharmacologic inhibition of S1P lyase by SLI decreased proliferation and activated differentiation of HEKn cells simultaneously. (omicsdi.org)
  • Her main areas of investigation are keratinocyte stem cells, (photo)aging and mechanisms of action of anti-aging molecules ranging from cell cycle and metabolism chemical/protein regulators to nutraceuticals. (skinsoc.org.sg)
  • Nucleated papillomas cells as well as both epidermal and follicular keratinocytes in hyperplastic skin contained increased pAkt compared to skin treated only with acetone or 7, 12 dimethylbenz[a] anthracene (DMBA). (uky.edu)
  • Psoriasin, which is highly expressed in psoriasis, is encoded by a gene located within the epidermal differentiation complex. (medicaljournals.se)
  • The aim of this study was to investigate the effect of endogenous psoriasin on disturbed keratinocyte differentiation in psoriasis. (medicaljournals.se)
  • Galectin-7 downregulation in lesional keratinocytes contributes to enhanced IL-17A signaling and skin pathology in psoriasis. (omicsdi.org)
  • Psoriasis is a chronic inflammatory skin disease characterized by inflammatory cell infiltration, as well as hyperproliferation of keratinocytes in skin lesions, and is considered a metabolic syndrome. (omicsdi.org)
  • We show that LUT-7G leads to a modification of the cell cycle and the induction of keratinocyte differentiation, with modification of energy, fatty acid, and redox metabolism. (omicsdi.org)
  • She is now a Principal Investigator in A*SRL, where her team studies the pathways that regulate human primary keratinocyte proliferation, differentiation and senescence in 2D culture and 3D organotypic skin models. (skinsoc.org.sg)
  • The virus replicates only during terminal differentiation of the superficial keratinocytes, which allows it to be shed into the mucosal lumen. (medscape.com)
  • The presence of carboxy terminal KDEL appears to be necessary forretention and appears to be sufficient to reduce the secretion of proteins from the ER. (exposed-skin-care.net)
  • Retinol and derivatives of retinol that play an essential role in metabolic functioning of the retina, the growth of and differentiation of epithelial tissue, the growth of bone, reproduction, and the immune response. (drugbank.com)
  • 1999. Response of normal human keratinocytes to sulfur mustard (HD): Cytokine release using a non-enzymatic detachment procedure. (cdc.gov)
  • To explore mechanisms whereby Malpighian keratinocytes can transdifferentiate into an intestinal-like epithelium, as observed in the early steps of Barrett's esophagus (BE) development, long-standing cultures of esophageal keratinocytes derived from normal mouse esophageal explants were developed. (silverchair.com)
  • Therefore, we investigated the effects of NAM in both normal and photoaging conditions, focusing on human primary keratinocyte phenotypes and mechanistic pathways. (skinsoc.org.sg)
  • Further cellular studies indicated that the modulation of TLR2/NF- kB signaling pathways mediated GMP's anti-inflammatory effects on keratinocytes. (cosmeticsbusiness.com)
  • It was shown to inhibit keratinocyte proliferation and promotes terminal differentiation 17 . (nature.com)
  • In 1987 she relocated to the Cancer Research UK London Research Institute (now part of the Francis Crick Institute) where she served as Head of the Keratinocyte Laboratory. (wikipedia.org)
  • Melanin granules are eventually degraded as the keratinocyte undergoes terminal differentiation. (biologiedelapeau.fr)
  • An unusual strain of human keratinocytes which do not stratify or undergo terminal differentiation in culture. (rupress.org)
  • Similar to the in vivo temiskaming shores situation, expression of p1c was maintained when cultured keratinocyte todmorden monolayers were induced to stratify and undergo terminal differentiation, raleigh whereas expression of p1a was down-regulated kostan et al. (hoselito.com)
  • In vitro studies have demonstrated normalization of lipid transport when the wild-type ABCA12 gene is transferred to keratinocytes of patients with harlequin ichthyosis. (medscape.com)
  • Dynamic Ppargc1a expression in the mouse hair cycle suggests a possible role in regulating hair growth and differentiation. (nature.com)
  • Inherited or sporadic genetic defects, possibly creating a change in immune function and/or keratinocyte function, are thought to be responsible for several forms of porokeratosis. (medscape.com)