Keratinocyte cultures from human cells cultured. Medical search. Frequent questions

We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
We investigated adverse effects of single-wall carbon nanotube s (SWCNT) using a cell culture of immortalized human epidermal keratinocytes (HaCaT). (cdc.gov)
After 18 h of exposure of HaCaT to SWCNT, oxidative stress and cellular toxicity were indicated by formation of free radicals, accumulation of peroxidative products, antioxidant depletion, and loss of cell viability. (cdc.gov)
Radiosensitisation caused by titanium dioxide nanoparticles (TiO 2 -NPs) is investigated using phantoms (PRESAGE ® dosimeters) and in vitro using two types of cell lines, cultured human keratinocyte (HaCaT) and prostate cancer (DU145) cells. (medsci.org)
Water-soluble proteins of royal jelly were fractionated and investigated for the proliferative and migratory effects on human epidermal keratinocytes (HaCaT) in an in vitro wound healing model. (biomedcentral.com)
A protein fraction, mainly containing major royal jelly proteins 2 (MRJP2), MRJP3 and MRJP7, stimulated proliferative and migratory activities in HaCaT cells without visible cytotoxicity. (biomedcentral.com)
It exerted the greatest effects on the growth of HaCaT cells in the first 48 h. (biomedcentral.com)
The LuSens cell line is an immortalised adherent cell line derived from HaCaT human keratinocytes stably transfected with a selectable plasmid. (europa.eu)
The HaCaT human keratinocytes (provided by RWTH, Aachen, Germany) were genetically modified at the Institute of Anatomy and Cell Biology of the RWTH, Aachen (laboratory of CJ Wruck). (europa.eu)
In in vitro experiment using HaCaT cells, we found that the S. lappa components, including alantolactone, caryophyllene, costic acid, costunolide and dehydrocostus lactone significantly decreased the expression of PI3Kca but not IL20Rβ in vitro . (molcells.org)

Vitamin D3 (cholecalciferol) regulates keratinocyte proliferation and differentiation mostly by modulating calcium concentrations and regulating the expression of genes involved in keratinocyte differentiation. (wikipedia.org)
The high expression level in psoriatic epidermis in conjunction with the disturbed differentiation process suggests that psoriasin is involved in keratinocyte differentiation. (medicaljournals.se)

Factors promoting keratinocyte differentiation are: A calcium gradient, with the lowest concentration in the stratum basale and increasing concentrations until the outer stratum granulosum, where it reaches its maximum. (wikipedia.org)

The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
In vitro, the expression of Hes1 was higher in confluent cells maintained under high calcium conditions. (molvis.org)
Anatase TiO 2 -NPs were synthesised, characterised and functionalised to allow dispersion in culture-medium for in vitro studies and halocarbons (PRESAGE ® chemical compositions). (medsci.org)
Comparable levels of radiosensitisation were observed in both phantoms and cells at kilovoltage ranges of x-ray energies (slightly higher in vitro) . (medsci.org)
In this study, we established an in vitro model based on a co-culture of primary human keratinocytes and differentiated SH-SY5Y cells, a human neuronal cell line. (bvsalud.org)
This co-culture model of keratinocytes and SH-SY5Y cells may be useful in vitro alternatives for studying the close communication between keratinocytes and neuronal cells and for screening therapeutic drugs for sensitive skin. (bvsalud.org)

Basal cells in the basal layer (stratum basale) of the skin are sometimes referred to as basal keratinocytes. (wikipedia.org)
In psoriasis, the over-proliferation of basal layer keratinocytes (KCs) is coupled with differentiation and cornification defects in the upper layers, leading to plaque formation ( 7 ). (frontiersin.org)
In this process, the keratinocytes progress from the basal layer of the epidermis through distinct stages to form the spinous, granular and cornified layers. (medicaljournals.se)

For example, seeding fibroblasts or keratinocytes results in faster vascularization due to growth factor secretion. (hindawi.com)

Excessive proliferation of keratinocytes, abnormalities in the differentiation process, and continuous shedding of the thickened epidermis are characteristics of psoriasis (Figures 1 (a) and 1 (b)) [ 2 ]. (hindawi.com)
The first occurs by migration and proliferation of keratinocytes from the wound edges and by differentiation of stem cells from remaining hair follicle bulbs. (worldwidewounds.com)

Those elevations of extracellular calcium concentrations induces an increase in intracellular free calcium concentrations in keratinocytes. (wikipedia.org)
The intracellular roles of NPM include a wide range of metabolic processes such as ribosome biogenesis, proliferation, cell cycle control, migration, DNA repair, non-programmed cell death, stress sensor ( 3 , 4 ). (frontiersin.org)
In the most well characterized "canonical" pathway in mammalian cells, the Notch intracellular domain (NotchIC) translocates to the nucleus where it mostly associates with the recombination signal binding protein for the immunoglobulin kappa J region (RBPJκ). (molvis.org)
Native honey (1% w/v pH 7.4, 10(6) cells) showed strong quenching activity against lipophilic cumoxyl and cumoperoxyl radicals, with significant suppression/prevention of cell damage, complete inhibition of cell membrane oxidation, of intracellular ROS production and recovery of intracellular GSH. (researchgate.net)
Adipose tissue is a loose connective tissue composed of cells supported by an intracellular matrix as well as by vascular, lymphatic, and neural networks. (jcadonline.com)
Intracellular psoriasin has been found to interact with various signalling proteins, including the nuclear factor (NF)-kB regulator Jab1, while secreted psoriasin functions as an antimicrobial peptide and has the ability to induce chemotaxis of neutrophils and T cells (6). (medicaljournals.se)
Previous studies of the role of psoriasin in keratinocyte differentiation have focused on the effects of extracellular psoriasin, but as the psoriasin in psoriasis is keratinocyte-derived, it is imperative to consider the effects of psoriasin as an endogenously produced intracellular mediator. (medicaljournals.se)

Previous reports have shown that VEGF overexpression in keratinocytes augments wound vascularization [ 13 ]. (hindawi.com)
SCARA5 overexpression significantly inhibited cell proliferation and induced apoptosis of CAL-27 and SCC-9 cells. (degruyter.com)
Psoriasin (S100A7) was first identified for its overexpression in psoriatic keratinocytes (3), where it was found to be located to the nucleus and the cytoplasm (4). (medicaljournals.se)

Keratinocytes differentiate from epidermal stem cells in the lower part of the epidermis and migrate towards the surface, finally becoming corneocytes and eventually be shed off, which happens every 40 to 56 days in humans. (wikipedia.org)
Epidermal stem cells reside in the lower part of the epidermis (stratum basale) and are attached to the basement membrane through hemidesmosomes. (wikipedia.org)
Epidermal stem cells divide in a random manner yielding either more stem cells or transit amplifying cells. (wikipedia.org)
Those stem cells and their differentiated progeny are organized into columns named epidermal proliferation units. (wikipedia.org)
In humans, it is estimated that keratinocytes turn over from stem cells to desquamation every 40-56 days, whereas in mice the estimated turnover time is 8-10 days. (wikipedia.org)
These factors include: The transcription factor p63, which prevents epidermal stem cells from differentiating into keratinocytes. (wikipedia.org)
Total and conditional knock out of PPARγ in hair follicle bulge stem cells showed similar result where scarring alopecia was observed in mice with the loss of pilosebaceous units and inflammatory cells infiltration 18 , 19 . (nature.com)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
Such continuously renewing epithelia are by definition governed by stem cells. (molvis.org)
Upon division, the slow cycling limbal epithelial stem cells give rise to daughter cells known as transient amplifying (TA) cells. (molvis.org)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. (bvsalud.org)
6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)

First, we cocultured dermal fibroblasts with dermal microvascular endothelial cells at a ratio of 2 : 1. (hindawi.com)
Briefly, dermal fibroblasts were cultured in the presence of ascorbic acid (50 μg/ml) for 35 days to promote the production of their own extracellular matrix (ECM). (nih.gov)

The aim of this study was to detect the expression of molecules associated with Notch signallng pathway in SHED cultured in specific differentiation medium, namely, KGM. (bvsalud.org)

Sustained hypoxia or lack of nutrients leads to fibroblast dysfunction, decreased keratinocyte migration and proliferation, and tissue loss [ 6 ]. (hindawi.com)
It activates both the EGF R/ErbB1 and ErbB4 receptors and has been linked to many cellular processes including proliferation, apoptosis, cell migration/invasion, differentiation, morphogenesis, and development. (rndsystems.com)
We propose a new computation-based approach for elucidating how signaling molecules are decoded in cell migration. (nature.com)
Using the response function, we could predict the morphological change from molecular activity and its predictive performance provides a new quantitative measure of how much the Rho GTPases participate in the cell migration. (nature.com)
Thus, our proposed predictive approach enabled us to uncover the hidden information processing rules of Rho GTPases in the cell migration. (nature.com)
To examine this issue, we focused on cell migration as a model biological system, because both molecular activity (as input signal) and morphological changes (as output signal) during cell migration can be monitored by FRET imaging. (nature.com)
Many studies have extensively investigated the molecular mechanisms involved in cell migration and have recognized the Rho small GTPases as key regulators of actin dynamics 7 , 8 . (nature.com)
In spite of such accumulating knowledge of Rac1 and Cdc42 downstream pathways, little is known about the functional differences between Rac1 and Cdc42, in particular, how these Rho GTPases participate in cell migration. (nature.com)
Moreover, a quantified discussion has been missing: although these molecules are involved in cell migration, how much are they quantitatively responsible for? (nature.com)

The aim of this work was to study the protective activity of a honey of multifloral origin, standardized for total antioxidant power and analytically profiled (HPLC-MS) in antioxidants, in a cultured endothelial cell line (EA.hy926) subjected to oxidative stress. (researchgate.net)
We have shown previously that extracellular psoriasin binds to the receptor for advanced glycation end products (RAGE), promoting endothelial cell proliferation (8). (medicaljournals.se)

Keratinization is part of the physical barrier formation (cornification), in which the keratinocytes produce more and more keratin and undergo terminal differentiation. (wikipedia.org)
To establish the skin barrier and the epidermal structure, keratinocytes undergo a tightly regulated process of differentiation. (medicaljournals.se)
Migratory lesions of ichthyosis linearis circumflexa may be caused by a dermal influx of inflammatory cells that undergo phagocytosis and digestion by keratinocytes, resulting in disruption of keratinization. (medscape.com)

Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)
However, these methods have some drawbacks because the ECM substitutes contained exogenous materials such as bovine collagen, dead human allogenic dermis, or synthetic polymers [ 16 ]. (hindawi.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
METHODS: Human epidermal keratinocytes and SH-SY5Y cells were monocultured and co-cultured. (bvsalud.org)

However, the engineering of more complex tissues consisting of large 3D structures remains a critical challenge because the penetration of oxygen, which is required for cell survival, is limited by diffusion to a distance of approximately 150 to 200 mm from the nearest blood vessel. (hindawi.com)
Thus, the long-term survival and function of 3D tissues depend on the rapid development of new blood vessels to provide nutrients and oxygen to cells in the center of the tissue grafts. (hindawi.com)
Reconstructed tissues were cultured for 7 days under submerged conditions in complete DME-HAM supplemented with ascorbate and then transferred to the air-liquid interface for 7 days in EGF-free DME-HAM to induce epithelial differentiation. (nih.gov)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
It has been found that some of the SVF cell types possess regenerative and anti-inflammatory potentials in damaged tissues due to their ability to secrete growth factors and anti-inflammatory molecules. (jcadonline.com)
11 However, the greater the abundance of adipose tissue in the human body, the significantly higher the yield of MSCs is compared to other tissues. (jcadonline.com)

Oral squamous cell carcinoma (OSCC) is a common tumor in the world. (degruyter.com)
SUMMARY BLURB Essential oil derived from the camphor tree acts by stimulating immune cell-dependent regression of skin tumors in a mouse model of cutaneous squamous cell carcinoma. (biorxiv.org)
In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)

Epithelial cells were then seeded on the fibrous sheet to assemble the bilayered tissue. (hindawi.com)
Tissue engineering performs the culture of cells on scaffolds, aiming at the restoration of damaged tissue. (springer.com)
Tumour radiosensitivity can be modified by agents, which enhance the radiation effects in the tumour, with the objective of delivering efficient radiation doses that can eradicate cancer cells without exceeding normal tissue tolerances [ 5 ]. (medsci.org)
For example, despite improvements in cell culture techniques and developments in dermal matrices, tissue-engineered skin substitutes have yet to achieve widespread use by clinicians. (worldwidewounds.com)
Diagnosis is clinical and microscopic, confirmed by culture or fixed-tissue staining. (msdmanuals.com)

Keratinocytes are the primary type of cell found in the epidermis, the outermost layer of the skin. (wikipedia.org)
In humans, they constitute 90% of epidermal skin cells. (wikipedia.org)
More recent skin substitutes combine epidermal and dermal layers by introducing fibroblasts and keratinocytes into an acellular matrix [ 5 ]. (hindawi.com)
Skin substitutes seeded with cells producing growth factor on a continuous basis provide an attractive alternative [ 12 ]. (hindawi.com)
Once T cells were added to the 3D skin model, the culture medium was supplemented with IL-2 (10 U/ml) and IL-23 (20 ng/ml) until the end of the culture period. (nih.gov)
At day 35, a fibroblast sheet with keratinocytes was superimposed on a T cell-containing fibroblast sheet, thus forming a skin substitute comprising a T-cell-enriched dermis and an epidermis. (nih.gov)
These newly formed skin substitutes were then raised at the air-liquid interface and kept in culture for a final 3 weeks in the keratinocytes culture medium depleted of EGF to allow complete differentiation of the epidermis. (nih.gov)
Exposure to SWCNT also resulted in ultrastructural and morphological changes in cultured skin cells. (cdc.gov)
Both the innate stimuli, such as lipopolysaccharides and Poly inositol-cytosine and adaptive stimuli, that is, cytokine mix, were able to induce the extracellular release of NPM in immortalized keratinocytes and human skin fibroblasts in the absence of cytotoxicity. (frontiersin.org)
One approach to protecting human skin against UV radiation is the use of antioxidants. (spandidos-publications.com)
Treatment with PF markedly reduced the production of ROS, and inhibited the activation of p38 and p53 in human keratinocytes, thus suggesting that the ROS‑p38‑p53 pathway has a role in UV‑B‑induced skin damage. (spandidos-publications.com)
Extracts of peony root have previously been shown to exert protective effects against UV-induced DNA damage and facial wrinkles in human skin ( 16 ). (spandidos-publications.com)
We tested an essential-oil derivative, camphor white oil (CWO), for anti-tumor activity in a mouse model of keratinocyte-derived skin cancer. (biorxiv.org)
Daily topical treatment with CWO induced dramatic regression of pre-malignant skin tumors and a two-fold reduction in cutaneous squamous cell carcinomas. (biorxiv.org)
We also confirmed that TTBC could be used for the treatment of sensitive skin through a co-culture model and in vivo tests. (bvsalud.org)
However, due to the abnormal pigments' synthesis, being responsible for skin color in accumulation of melanin, the formation of pigment humans. (bvsalud.org)
A spontaneously transformed human epithelial cell line developed from a long-term primary culture of human adult skin KERATINOCYTES. (bvsalud.org)

It was shown to inhibit keratinocyte proliferation and promotes terminal differentiation 17 . (nature.com)
Pretreatment of cells with SB202190, a p38 inhibitor, may inhibit the DNA binding activity of p53 and suppress p53-dependent transcription ( 10 ). (spandidos-publications.com)

The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)

1) Some of the known effects of LLLT include the enhancement of ATP production, which acts as the trigger for the biostimulating effect,(2) improved microcirculation and bone regeneration, (3) and accelerated wound healing in human sports injuries and accidents. (atcpainreliefdr.com)

Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
The aim of this study was to investigate the effect of endogenous psoriasin on disturbed keratinocyte differentiation in psoriasis. (medicaljournals.se)
In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)

The morphological analysis, carried out on the third and fifth days, did not verify changes in the typical morphology of Vero cells cultured with SPRP. (springer.com)
In this approach, we performed FRET time-lapse imaging of Rac1 and Cdc42, members of Rho GTPases which are responsible for cell motility and quantitatively identified the response functions that describe the conversion from the molecular activities to the morphological changes. (nature.com)

During this differentiation process, keratinocytes permanently withdraw from the cell cycle, initiate expression of epidermal differentiation markers, and move suprabasally as they become part of the stratum spinosum, stratum granulosum, and eventually corneocytes in the stratum corneum. (wikipedia.org)
In addition, the gene that encodes psoriasin is positioned within the epidermal differentiation complex (EDC) (10-12), a gene cluster that contains several genes of importance in the terminal differentiation of the human epidermis (10, 11). (medicaljournals.se)

T cells were then cultured for 7 days at 37°C in an 8% CO2 atmosphere with various cytokines to induce their polarization in Th1 and Th17 cells. (nih.gov)
Autologous preparations rich in growth factors promote proliferation and induce VEGF and HGF production by human tendon cells in culture. (springer.com)
The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)

In an anagen human hair follicle PPARγ expression is detected in the mesenchymal DP cells, epithelial cells of the outer root sheath (ORS), inner root sheath and matrix 16 . (nature.com)
In addition, SCARA5 repressed OSCC cell epithelial-mesenchymal transformation (EMT), evidenced by increased E-cadherin expression and reduced N-cadherin expression. (degruyter.com)
We previously showed that genotoxic stress induced an active extracellular release of nucleophosmin (NPM) in human cardiac mesenchymal progenitor cells, and that serum deprivation provokes NPM secretion from human endothelial cells, eliciting inflammation via nuclear factor kappa B (NF-kB) transcriptional activation. (frontiersin.org)

Two fibroblasts sheets were then superimposed to form a reconstructed stroma, on which keratinocyte were seeded. (nih.gov)
Microscopic examination showed a cord-like arrangement of plasmacytoid cells within a myxoid stroma. (bvsalud.org)

in addition, the poor take rate of keratinocyte grafts has been a major problem. (worldwidewounds.com)

The keratinocytes in active psoriatic lesions display an altered sequence and altered expression levels of the differentiation markers (2). (medicaljournals.se)
However, the mediators that are responsible for the dysregulated differentiation of psoriatic keratinocytes have not been identified. (medicaljournals.se)

Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
There was also no translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus in apoptotic keratinocytes, indicating Tan IIA-induced apoptosis occurs mainly through the caspase pathway. (hindawi.com)
In conclusion, SCARA5 inhibited the proliferation and EMT and induced the apoptosis of OSCC cells through the inhibition of STAT3 and PI3K/AKT signaling pathways, thereby exerting a tumor suppressor effect. (degruyter.com)
In addition, cell death analysis indicated that PF treatment markedly reduced UV‑B‑radiation‑induced apoptosis in keratinocytes, which was accompanied by increased procaspase 3 expression and decreased cleaved caspase 3 expression. (spandidos-publications.com)
UV-B-induced DNA damage can trigger cell signaling pathways to activate defense systems, leading to DNA repair and/or apoptosis ( 6 ). (spandidos-publications.com)

The evaluation of SPRP as a supplement showed that there was no statistical difference in cell viability compared to cultures supplemented in the standard way, with fetal bovine serum (FBS), after 24 h of culture. (springer.com)
Clonogenic and cell viability assays were employed to determine cells survival curves from which the dose enhancement levels "radiosensitisation" are deduced. (medsci.org)
Cell viability assay also revealed that the extracts could safely be used in concentrations of 125 µg/mL. (bvsalud.org)

Although, most of these studies have focused on treatment of tumors, the effects and mechanism of action in keratinocytes are still poorly understood. (hindawi.com)

The results of the present study demonstrated that treatment with PF (25, 50, and 100 µM) significantly increased the percentage of viable keratinocytes after UV‑B exposure. (spandidos-publications.com)
Moreover, the expression of inflammatory cytokines and neuropeptides was significantly increased in co-cultured keratinocytes compared to that under monoculture conditions. (bvsalud.org)

Since keratinocyte differentiation inhibits keratinocyte proliferation, factors that promote keratinocyte proliferation should be considered as preventing differentiation. (wikipedia.org)

ELISA demonstrated that vascularization was promoted by significant upregulation of vascularization associated growth factors due to interactions among the 3 types of cells in the bilayer, as compared to cocultures of fibroblast and endothelial cells and monocultures. (hindawi.com)
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Heparin Binding EGF-like Growth Factor (HB-EGF). (rndsystems.com)
HB-EGF (Heparin-Binding EGF-like growth factor), also known as DTR, is produced by bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes, and IL-10-secreting regulatory macrophages. (rndsystems.com)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
Cultured human keratinocytes, endothelial cells and fibroblasts, when exposed to laser, caused the production of transforming growth factor, (9) and laser increases the transforming of fibroblasts into myofibroblasts. (atcpainreliefdr.com)
The energy density of 3 J/cm2 remarkably increased cell growth, with no effect on procollagen synthesis, as demonstrated by immunoprecipitation analysis. (atcpainreliefdr.com)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)

More than 40 distinct SPINK5 mutations have been reported to the Human Gene Mutation Database, with nonsense, splicing, and small insertions/deletions comprising the majority of them. (medscape.com)

Calcium concentration in the stratum corneum is very high in part because those relatively dry cells are not able to dissolve the ions. (wikipedia.org)
In cultured keratinocytes, CWO stimulated calcium signaling, resulting in calcineurin-dependent activation of nuclear factor of activated T cells (NFAT). (biorxiv.org)
Changes in calcium influx, substance P, inflammatory cytokines, and neuropeptides between the monoculture and co-culture groups treated with capsaicin only and capsaicin with transient receptor potential channel vanilloid subfamily member 1 (TRPV1) antagonist, trans-4-tert-butylcyclohexanol (TTBC), together. (bvsalud.org)

In addition, UV induces the generation of reactive oxygen species (ROS), which overwhelm the antioxidant defense system, resulting in oxidative stress and damage to cell structure, DNA, proteins and lipids ( 5 ). (spandidos-publications.com)
Pretreatment of cells with N-acetyl-L-cysteine (NAC), an agent known to counteract oxidative stress, attenuates the cellular p53 response to UV ( 4 ). (spandidos-publications.com)

Pathogens invading the upper layers of the epidermis can cause keratinocytes to produce proinflammatory mediators, particularly chemokines such as CXCL10 and CCL2 (MCP-1) which attract monocytes, natural killer cells, T-lymphocytes, and dendritic cells to the site of pathogen invasion. (wikipedia.org)
Some of the transit amplifying cells continue to proliferate then commit to differentiate and migrate towards the surface of the epidermis. (wikipedia.org)

Induction of keratinocyte differentiation caused concurrent expression of psoriasin and the differentiation marker involucrin. (medicaljournals.se)
Relevant of mutagenicity and clastogenici- angiosarcomas of the liver, which carcinogens discussed in this chap- ty, including the induction of sister are rare tumours, were identified in ter do not include pharmaceutical chromatid exchange (SCE), chro- humans, rats, and mice exposed to drugs classified in Group 1, which mosomal aberrations (CA), and mi- vinyl chloride. (who.int)

This is supported by reduced proliferation of human dermal papilla and predominantly epithelial keratinocytes after incubation with AICAR, the agonist for AMPK signaling which activates PPARGC1a and serves as co-activator of PPARγ. (nature.com)
Expression of Notch1 , Jagged1 , and Hes1 was found predominantly in the basal and immediate suprabasal cells. (molvis.org)

Microarray analysis is a molecular technique that enables the parallel analysis of expression by a very large number of genes encompassing a significant fraction of the human genome. (molcells.org)

Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
In vivo , CWO induced transcriptional changes in immune-related genes, resulting in cytotoxic T cell-dependent tumor regression. (biorxiv.org)

During neonatal corneal development, NotchIC was detected in occasional cells at P10 while at P15 and P90, it was found in the basal and early suprabasal layers. (molvis.org)

Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular ardiomyoplasty. (springer.com)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)

Later studies have demonstrated secretion of psoriasin from epithelial cells (5). (medicaljournals.se)

Exposure to carbon nanotube material: assessment of nanotube cytotoxicity using human keratinocyte cells. (cdc.gov)

Intradermal adipocyte progenitor cells are activated toward the end of the telogen phase to proliferate and differentiate into mature adipocytes. (jcadonline.com)

Interestingly, NPM interacts with Toll-like receptor (TLR)4 in these cells and activates an NF-kB-dependent inflammatory pathway upregulating interleukin IL-6 and COX-2 gene expression. (frontiersin.org)
[ 19 ] studied steady-state levels of the mRNA encoding LEKTI (lymphoepithelial Kazal-type related inhibitor), the gene product of the SPINK5 gene, in cultured epidermal keratinocytes from a healthy control and 5 Netherton syndrome patients. (medscape.com)

The confluent cells will be incubated at 37 ± 1.5 °C and 5.0 ± 0.5 % CO2 (standard cell culture conditions). (europa.eu)

Therefore, the parameters of the experiments remain similar, because of the reproducible characteristics of the cells. (europa.eu)

Corneocytes are keratinocytes that have completed their differentiation program and have lost their nucleus and cytoplasmic organelles. (wikipedia.org)

All of the chemicals discussed of this chemical was raised to carci- lung and the nasal cavity were iden- here are IARC Group 1 agents and nogenic to humans (Group 1) based tified as target organs in humans as such can be characterized as on strong mechanistic evidence and rats, respectively. (who.int)

Among these cell-signaling proteins, activation of p53 is important in cells with UV radiation-induced DNA damage ( 4 , 7 ). (spandidos-publications.com)
We fractionated the water-soluble proteins of royal jelly and identified one fraction (Fraction 2) that induced both proliferative and migratory effects on a human epidermal keratinocyte cell line. (biomedcentral.com)

Brunner D, Frank J, Appl H, Schöffl H, Pfaller W, Gstraunthaler G. Serum-free cell culture: the serum-free media interactive online database. (springer.com)

When cells attained 75-95% confluence, they were passaged. (nih.gov)
The medium was changed 3 days after culture and were sub-cultured once they reached 70% confluence. (bvsalud.org)

The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. (springer.com)

The corneal epithelium is continually losing cells due to desquamation (primarily via the blink reflex) and thus must replace these cells to maintain homeostasis. (molvis.org)

Anatomy10

Diseases4

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes15

Information Science3

Health Care1

HaCat10

Involved in keratinocyte differentiation2

Promoting keratinocyte differentiation1

Vitro8

Basal layer3

Fibroblasts or keratinocytes1

Proliferation of keratinocytes2

Intracellular7

Overexpression3

Stem cells18

Dermal Fibroblasts2

Specific differentiation1

Migration9

Endothelial cell2

Undergo3

Methods5

Tissues7

Squamous cell carc3

Tissue5

Skin17

Inhibit2

Regulates1

Wound1

Psoriasis3

Morphological2

Epidermal differentiation2

Induce3

Mesenchymal3

Stroma2

Grafts1

Psoriatic2

Apoptosis5

Viability3

Poorly understood1

Significantly increased2

Inhibits1

Growth7

Mutations1

Calcium3

Oxidative stress2

Epidermis2

Induction2

Predominantly2

Molecular1

Vivo2

Suprabasal1

Cellular2

Secretion1

Cytotoxicity1

Differentiate1

Gene2

Confluent cells1

Characteristics1

Nucleus1

Organs1

Proteins2

Serum1

Confluence2

Differentiation and proliferation1

Desquamation1