Keratin 15 antibody. Medical search. Frequent questions

Keratins act as cytoprotective proteins. (sigmaaldrich.com)
Their mechanism of action is dominant negative interference, with the mutated keratin proteins interfering with the structure and integrity of the cytoskeleton. (wikipedia.org)
The sulfhydryl groups of cysteines in the head region of the keratin proteins 1 (K1) and 10 (K10) are likely targets. (cdc.gov)
To determine the antibody responses over time in patients and healthy controls against host tissue proteins - cardiac myosin and peptides from its S2 subfragment, tropomyosin, laminin and keratin.Methodology. (bvsalud.org)
We observed significantly higher antibody responses against extracellular proteins - laminin and keratin in pharyngitis group, patients with ARF and patients with RHD when compared to healthy controls. (bvsalud.org)
The antibody responses against intracellular proteins - cardiac myosin and tropomyosin were elevated only in the group of patients with ARF with active carditis. (bvsalud.org)
Our findings of antibody responses against host proteins support the multistep hypothesis in the development of rheumatic carditis. (bvsalud.org)
The Anti-Cytokeratin antibody has a primary reactivity with human cytokeratin proteins that correspond to Moll's peptide #7 (cytokeratin 7) and Moll's peptide #8 (cytokeratin 8), 54 and 53 kilodaltons (kDa), respectively. (bdbiosciences.com)
The members from the large keratin family of cytoskeletal proteins are expressed in a carefully regulated tissue- and differentiation-specific manner. (exposed-skin-care.net)
Keratins are a large family of proteins which form the intermediate filament (IF) cytoskeleton of epithelial cells and their appendages hairs and nails (reviewed in references 8 and 15). (exposed-skin-care.net)
These proteins are subdivided according to biochemical criteria into two subfamilies: type I or acidic keratins and type II or neutral-basic keratins. (exposed-skin-care.net)
Like all IF proteins keratins consist of a central α-helical rod domain responsible for dimerization and higher-order polymerization. (exposed-skin-care.net)
Variations in these nonhelical end domains largely account for the differences between individual keratin proteins. (exposed-skin-care.net)
The variety of keratin genes differentially expressed suggests that these proteins may however have additional functions. (exposed-skin-care.net)
Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS), we identified 42 proteins in complex with APC, including β-catenin and intermediate filament (IF) proteins lamin B1 and keratin 81. (ku.edu)
APC also colocalized with keratins and remained associated with IF proteins throughout a sequential extraction procedure. (ku.edu)
The antibodies are proteins in different patterns, without which the defense mechanism in the body cannot function at all. (newhealthadvisor.org)
Proteins provide 10-15% of calorie intake per day. (newhealthadvisor.org)
Keratin ( / ˈ k ɛr ə t ɪ n / [1] [2] ) is one of a family of structural fibrous proteins also known as scleroproteins . (wikipedia.org)
Professor Birgitte Lane's research career has been distinguished for her contribution to the understanding of the structure and function of the keratin cytoskeleton , the subcellular system of nanofilamentous proteins that give tissues their resilience and strength, with special focus on the biology of the skin. (skinsoc.org.sg)
During the initial infection, alpha-helical M proteins (M8 and M13) on the surface of the streptococcus bind type IV collagen in the host, and this interaction can trigger auto-antibody formation. (medscape.com)
9] ACCP antibodies are a subset of ACPA and are not completely cross-reactive with other citrullinated proteins. (medscape.com)
Citrulline modified proteins are seen in the keratin layer of the epidermis and the brain. (medscape.com)

Biology of disease classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications and limitations. (bdbiosciences.com)
She pioneered the development and use of monoclonal antibodies to keratins as markers of differentiation in health and disease, and key tools in diagnostic pathology. (skinsoc.org.sg)

Polyclonal rabbit antibodies produced against the naphthyl-modified keratins reacted with their respective antigens with threshold sensitivities of 15-31 ng/mL and high specificity over a linear range up to 500 ng/mL. (cdc.gov)
Battifora H. Diagnostic uses of antibodies to keratins: A review and immunohistochemical comparison of seven monoclonal and three polyclonal antibodies. (bdbiosciences.com)

11] Although multiple assays are available, including antimutated citrullinated vimentin antibody and several generations of anti-CCP, they have all been shown to have comparable diagnostic performance. (medscape.com)

Immunoglobulin A RF and the co-presence of RF with anti-keratin or anti-filaggrin antibodies may increase levels of prediction. (nih.gov)

The neutral-basic keratins are encoded on chromosome 12 (12q13.13). (wikipedia.org)

It dimerizes with keratin 14 and forms the intermediate filaments (IF) that make up the cytoskeleton of basal epithelial cells. (wikipedia.org)
Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. (wikipedia.org)
Although the primary function of keratin PD1-PDL1 inhibitor 2 PD1-PDL1 inhibitor 2 IF has long been thought of as structural this hypothesis was not confirmed until the discovery that keratin mutations result in diseases characterized by epithelial fragility (reviewed in references 3 5 6 and 15. (exposed-skin-care.net)
Histologically, the fully formed domelike lesion reveals a mass of proliferating squamous epithelial cells, which created the central core of keratin. (medscape.com)
Keratin also protects epithelial cells from damage or stress. (wikipedia.org)
For example, mouse thymic epithelial cells react with antibodies for keratin 5, keratin 8, and keratin 14. (wikipedia.org)
These antibodies are used as fluorescent markers to distinguish subsets of mouse thymic epithelial cells in genetic studies of the thymus . (wikipedia.org)
SM-AP1-3 cells were immunopositive for keratin only, indicating their duct-epithelial or squamous cell differentiation, while SM-AP4/5 cells were positive for both keratin and S-100 protein, indicating their myoepithelial cell differentiation. (biomedcentral.com)
Expression of keratin 5 as a distinctive feature of epithelial and biphasic mesotheliomas. (novodiax.com)
For example, the epithelial−mesenchymal transition phenotype presents more cells with a lower degree of differentiation and greater cell dissociation in the ITF than in other tumor areas 14 , 15 . (nature.com)

The severity of EBS has been observed to be dependent upon the position of the mutation within the protein, as well as the type of keratin (K5 or K14) that contains the mutation. (wikipedia.org)
Alpha-keratin (α-keratin) is a type of keratin found in vertebrates . (wikipedia.org)

Mouse monoclonal antibody to BiP/GRP78. (exposed-skin-care.net)
Human IgG antibody Laboratories manufactures the mouse monoclonal keratin 14 irwin mclean/irene leigh reagents distributed by Genprice. (diastrip.com)
The Mouse Monoclonal Keratin 14 Irwin Mclean/Irene Leigh reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (diastrip.com)
Description: A Mouse monoclonal antibody against Rat Keratin 4 (KRT4). (diastrip.com)
Description: A Mouse monoclonal antibody against Human Keratin 5 (KRT5). (diastrip.com)
ProductData": { "ID": "RTD000669", "ProductType": "Others", "BrandName": "anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody", "ProductNameAddition": "", "RegulatoryDisclaimer1": "For in vitro diagnostic use. (roche.com)
Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Intended Use", "Value": "Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody is intended for laboratory use in the qualitative immunohistochemical detection of cytokeratin 5 by light microscopy in sections of formalin-fixed, paraffin-embedded tissue stained on a BenchMark IHC/ISH instrument. (roche.com)
One 5 mL dispenser of anti-Cytokeratin 5/6 (D5/16B4) antibody contains approximately 52 μg of a mouse monoclonal antibody. (roche.com)
Language": "en", "Country": "XG", "Code": "Content" }, { "Name": "Background Information", "Value": "Anti-Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody (anti-Cytokeratin 5/6 (D5/16B4) antibody) is a mouse monoclonal antibody raised for the detection of cytokeratin (CK) 5. (roche.com)

Mutations that occur at either of the two 10-15 residue "hotspot" regions located on either end of the central rod domain (HIM and HTM) tend to coincide with more severe forms of EBS, whereas mutations at other spots usually result in milder symptoms. (wikipedia.org)
She established the link between keratin gene mutations and human skin fragility diseases , and led a team that identified the basis of more than 15 distinct inherited skin disorders. (skinsoc.org.sg)

dashed line histogram) or BD Horizon BV421 Mouse Anti-Human Cytokeratin antibody (Cat. (bdbiosciences.com)
The Anti-Cytokeratin antibody, clone CAM 5.2, is derived from the hybridization of P3/NS-1/1-Ag4-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with the human colorectal carcinoma cell line HT29. (bdbiosciences.com)
Makin C, Bobrow L, Bodmer W. Monoclonal antibody to cytokeratin for use in routine histopathology. (bdbiosciences.com)
Language": "en", "Country": "XG", "Code": "Intended Use" }, { "Name": "Content", "Value": "Anti-Cytokeratin 5/6 (D5/16B4) antibody contains sufficient reagent or 50 tests. (roche.com)
Anti-Cytokeratin 5/6 (D5/16B4) antibody is a monoclonal antibody produced as cell culture supernatant. (roche.com)
4,5 The immunohistochemistry (IHC)-based detection of CK5 with anti-Cytokeratin 5/6 (D5/16B4) antibody can be used to aid in the distinction between pulmonary SCCA and pulmonary adenocarcinoma (ADC). (roche.com)
In addition, CK5 is expressed in mesothelial cells of the pulmonary pleura and is overexpressed in epithelioid malignant mesothelioma (MM). 6 CK5 is consistently absent in pulmonary ADC that confounds the diagnosis of epithelioid MM. 6,7 Thus, detection of CK5 with anti-Cytokeratin 5/6 (D5/16B4) antibody can be used to aid in the differential diagnosis of epithelioid MM from lung ADC. (roche.com)
9,10 Thus, detection of CK5 in prostatic basal cells with anti-Cytokeratin 5/6 (D5/16B4) antibody may be used to aid in the differentiation of benign and malignant prostate lesions. (roche.com)
13-15 The detection of CK5 in breast MECs with anti-Cytokeratin 5/6 (D5/16B4) antibody may be used to aid in distinguishing non-invasive from invasive breast neoplasms. (roche.com)

Furthermore, increased epidermal thickness and keratin 6 (K6), K16, and K17 expression occurred in the ears and backs of C3aR-/- mice. (bvsalud.org)
Keratin monomers assemble into bundles to form intermediate filaments , which are tough and form strong unmineralized epidermal appendages found in reptiles , birds , amphibians , and mammals . (wikipedia.org)
Monoclonal Antibody Analysis of Keratin Expression in Epidermal Diseases: A 48- and 56-kdalton Keratin as Molecular Markers for Hyperproliferative Keratinocytes. (novodiax.com)
Immunohistocytochemical examination of the skin reveals characteristic abnormalities in the structure of lamellar granules and in the expression of epidermal keratin. (medscape.com)
For patients with locally advanced or metastasized SCCs treatment with cetuximab, a monoclonal antibody against epidermal growth factor receptor (EGFR), has been proposed and so far, treatment of five DEB patients with cetuximab has been published. (biomedcentral.com)

The disease is characterized by development of follicular papules and keratin spines (spicules) predominantly in the face, often accompanied by alopecia of the eyebrows and eyelashes. (cdc.gov)

Keratin 5, also known as KRT5, K5, or CK5, is a protein that is encoded in humans by the KRT5 gene. (wikipedia.org)
Keratin 5, like other members of the keratin family, is an intermediate filament protein. (wikipedia.org)
We hypothesized that electrophilic arene oxides formed by CYP isoforms expressed in the human skin react with nucleophilic sites on keratin, the most abundant protein in the stratum corneum that is synthesized de novo during keratinocyte maturation and differentiation. (cdc.gov)
Using different approaches we also found that the molecular system where these keratins modulate cell development appears to be associated with pRb as well as the molecular equipment controlling cell routine development during G1 which regarding K10 this function resides in the nonhelical termini from the protein. (exposed-skin-care.net)
Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex. (molvis.org)
Hair is made up of a protein called keratin. (newhealthadvisor.org)
Spider silk is classified as keratin, [9] although production of the protein may have evolved independently of the process in vertebrates. (wikipedia.org)
The new term corneous beta protein (CBP) has been proposed to avoid confusion with α-keratins. (wikipedia.org)
The antibody is diluted in a phosphate buffered saline containing carrier protein and 0.10% ProClin 300, a preservative. (roche.com)
We have recently identified and characterized keratin-18 (K18) and high mobility group box-1 protein (HMGB1) released from dying hepatocytes in a murine model of APAP hepatotoxicity as sensitive mechanism-based biomarkers, and we observed a hepatoprotective role played through induction of hepatocyte apoptosis (6). (islamophobiacon.com)
Production of anticitrullinated protein antibody (ACPA) depends on the genetic background of the patient. (medscape.com)
3, 4, 5, 6, 7] Anticitrullinated protein antibody (ACPA) level was added to the 2010 American College of Rheumatology(ACR)/European League Against Rheumatism (EULAR) diagnostic criteria for rheumatoid arthritis. (medscape.com)
15] Anti-CCP was found to be more predictive of erosive arthritis than other measures, such as matrix metalloproteinases-3, erythrocyte sedimentation rate, and C-reactive protein. (medscape.com)
Coenen D, Verschueren P, Westhovens R, Bossuyt X. Technical and diagnostic performance of 6 assays for the measurement of citrullinated protein/peptide antibodies in the diagnosis of rheumatoid arthritis. (medscape.com)

From a structural point of view we have recently reported that different keratin polypeptides even those belonging to the same expression pair display distinct dynamics in cell hybrids (19). (exposed-skin-care.net)
Immunolocalization of Keratin Polypeptides in Human Epidermis Using Monoclonal Antibodies. (novodiax.com)

Anti-S-phenylcysteine antibodies were not sufficiently specific or sensitive toward the target antigens for use in ELISA under our experimental conditions. (cdc.gov)
The body releases antibodies or immunoglobulins as a response to the presence of foreign antigens. (newhealthadvisor.org)

An immunohistochemical study using monoclonal antibody. (novodiax.com)

These heterodimers are formed between specific pairs of type I (acidic) and type II (basic) keratin. (wikipedia.org)
To gain a deeper insight into the diversity of keratin functions we have transfected human HaCaT keratinocytes with plasmids coding for several acidic keratins. (exposed-skin-care.net)
The acidic keratins are encoded on chromosome 17 (17q21.2). (wikipedia.org)

13-15 In cases where the MEC layer is difficult to evaluate via histology alone, ancillary IHC assays for MEC markers can be used. (roche.com)
Here, you will find references to external, peer-reviewed publications and textbooks that discuss some of the antibodies/ biomarkers utilized in our assays. (novodiax.com)

Keratin 5 (and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. (wikipedia.org)

This antibody is labeled with FITC. (diastrip.com)
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD24 Antibody, Clone M1/69, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530 (Catalog #60077), followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram). (stemcell.com)

Keratin 5-Cre-driven excision of nonmuscle myosin IIA in early embryo trophectoderm leads to placenta defects and embryonic lethality. (shengsci.com)

There is no known nonspecific antibody reactivity observed in this product. (roche.com)

This antibody is intended for in vitro diagnostic (IVD) use. (roche.com)

This deficiency may be overcome by SS of the entire lymph nodes and staining with a specific monoclonal antibody. (nih.gov)

By contrast antibodies against laminin and keratin persisted in patients with RHD.Conclusion. (bvsalud.org)
Most of these methods involve culturing endothelial cells on or within ECM substrates (e.g., collagen, fibrin, fibronectin, laminin, etc.) or other types of biomaterials to form capillary-like structures [ 15 ]. (hindawi.com)

b Quantification of the mean EGFR staining in tumor sections after normalization to keratin 14 staining in adjacent sections. (biomedcentral.com)
Anticyclic citrullinated peptide (anti-CCP) antibody and other autoantibody markers can be helpful in determining which patients with rheumatoid arthritis may have benefit from treatments such as anti-tumor necrosis factor-alfa (TNFa) monoclonal antibodies. (medscape.com)

S-arylcysteine--keratin adducts as biomarkers of human dermal exposure to aromatic hydrocarbons. (cdc.gov)

This division also has important structural and functional implications since to build up a well-organized IF cytoskeleton tetramers containing equimolar amounts of each keratin subtype are required. (exposed-skin-care.net)

The differential kinetics of serum antibody responses against S2 peptides may have potential use as markers of ongoing cardiac damage that can be used to monitor patients with ARF/RHD. (bvsalud.org)
13-15 Several MEC markers exist and recommendations include use of IHC studies for at least two markers to demonstrate the presence or absence of myoepithelial cells. (roche.com)

In dermal tape-strip samples collected from 13 individuals exposed to naphthalene-containing jet fuel, naphthyl-conjugated peptides were detected at levels from 0.343 +/- 0.274 to 2.34 +/- 1.61 pmol adduct/ug keratin but were undetectable in unexposed volunteers. (cdc.gov)
Patient's donor-specific antibodies (DSA) are undetectable. (cytopathology.ca)

The presence of specific pairs of type I/type II keratins (the so-called expression pairs) in different epithelia is highly regulated in a cell type- and differentiation-specific manner. (exposed-skin-care.net)
CD24 is involved in regulating B cell apoptosis and in preventing terminal differentiation of B cells into antibody-secreting plasmablasts and plasma cells. (stemcell.com)

The following synthetic S-arylcysteines were incorporated into 10-mer head sequences of K1 [GGGRFSS(S-aryl-C)GG] and K10 [GGGG(S-aryl-C)GGGGG] to form the predicted immunogenic epitopes for antibody production for ELISA: S-phenylcysteine-K1 (SPK1), S-phenylcysteine-K10 (SPK10), S-(1-naphthyl)cysteine-K1 (1NK1), S-(1-naphthyl)cysteine-K10 (1NK10), S-(2-naphthyl)cysteine-K1 (2NK1), and S-(2-naphthyl)cysteine-K10 (2NK10). (cdc.gov)

Using the source a bit more to explain what each gene and each zone of genes mean will be helpful, as we currently have no particular examples of a hair keratin. (wikipedia.org)
The human genome encodes 54 functional keratin genes , located in two clusters on chromosomes 12 and 17. (wikipedia.org)

K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. (wikipedia.org)

The antiserum was produced against synthesized peptide derived from human Keratin 8. (sigmaaldrich.com)
Eight human polyomaviruses have been identified: BKV ( 13 ), JCV ( 14 ), KIV ( 15 ), WUV ( 16 ), MCPyV ( 17 ), human polyomavirus type 6 (HPyV6) and type 7 (HPyV7) ( 18 ), and TSV ( 9 ). (cdc.gov)
The human keratins: biology and pathology. (novodiax.com)

Group A strep pharyngitis is most common in children 5-15 years old, but can occur in persons of any age. (medscape.com)

There are similar findings for predictors of functional disability in studies followed for 2-15 yr. (nih.gov)

The antibody clone D5/16B4, raised for the detection of CK5, is also reactive to CK6, another type II CK (60 kDa) that shares 86% sequence homology to CK5. (roche.com)

1988:1-15. (bdbiosciences.com)

Keratin 5 serves as a biomarker for several different types of cancer, including breast and lung cancers. (wikipedia.org)

Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. (bdbiosciences.com)

Below is a list of some internally validated antibodies by Applied Pathology Systems. (appliedpathology.com)

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. (bdbiosciences.com)

Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. (curehunter.com)

14] Additionally, anti-CCP antibodies have been shown to be predictive of the progression of patients, indicating more erosive disease or increase joint involvement. (medscape.com)

[11] Recent scholarship has shown that sauropsid β-keratins are fundamentally different from α-keratins at a genetic and structural level. (wikipedia.org)

The antibodies prevent infections and illnesses, and speed up recovery from diseases. (newhealthadvisor.org)

Results: We generated an APC antibody (APC-M2 pAb) raised against the 15 amino acid repeat region, and verified its reliability in applications including immunoprecipitation, immunoblotting, and immunofluorescence in cultured cells and tissue. (ku.edu)
KOH dissolves keratin found in cellular material and freeing fungal hyphae and yeast cells. (slideshare.net)
Many cell types have been implicated in determining the extent of organ injury or regeneration in the liver, such as Kupffer cells (14), neutrophils (15), natural-killer cells LAMA5 and natural-killer cells with T-cell receptor (16). (islamophobiacon.com)
These tubular cells also stained positive by an antibody against SV40 large T antigen, confirming the presence of polyomavirus cytopathic effects. (cytopathology.ca)
The cells are positive for keratin by immunoperoxidase staining. (xlbiotec.com)

Anatomy27

Organisms13

Diseases16

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes39

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science3

Health Care4

Proteins23

Monoclonal antibodies to keratins2

Polyclonal2

Vimentin1

Anti-Keratin1

Neutral-basic keratins1

Epithelial10

Type of keratin2

Mouse monoclonal9

Mutations2

Cytokeratin9

Epidermal5

Follicular papules1

Protein14

Polypeptides2

Antigens2

Immunohistochemical study1

Acidic3

Assays2

Epithelium1

FITC2

Myosin1

Reactivity1

Diagnostic1

Lymph nodes1

Laminin2

Tumor2

Biomarkers1

Subtype1

Markers2

Undetectable2

Differentiation2

ELISA1

Genes2

Filament1

Human3

Pharyngitis1

Functional1

Clone1

19881

Cancers1

Expression1

Pathology1

Affinity1

Coiled-coil1

Additionally1

Genetic1

Diseases1

Cells5