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  • lymphocytes
  • Imparting these modifications to GlyCAM-1, together with appropriate fucosylation, yields enhanced rolling ligands for both peripheral blood lymphocytes and Jurkat cells in flow chamber assays as compared with those generated with exogenous fucosyltransferase. (nih.gov)
  • Either sulfation modification results in an increased number of tethered and rolling lymphocytes, a reduction in overall rolling velocity associated with more frequent pausing of the cells, and an enhanced resistance of rolling cells to detachment by shear. (nih.gov)
  • To investigate the requirement for CD2 expression in activation of T lymphocytes via the CD3-Ti antigen/MHC receptor complex, we produced and characterized a series of CD2- Jurkat variants. (rupress.org)
  • Assuming that the Jurkat cell line is representative of normal cycling human T lymphocytes, we conclude that the presence of the CD2 molecule on the plasma membrane is not in itself a requirement for an operational CD3-Ti-alpha/beta receptor. (rupress.org)
  • cytotoxicity
  • Pycnidione irreversibly abrogated bleomycin-induced G2 arrest in Jurkat cells and synergically potentiated the cytotoxicity of bleomycin. (sigmaaldrich.com)
  • The resistance of Bak-deficient cells to GrB/Ad cytotoxicity was reversed by transduction of the Bak gene into these cells. (nih.gov)
  • To date, xCELLigence has not been able to monitor the growth or cytotoxicity of nonadherent cells derived from hematological malignancies. (nih.gov)
  • Drug cytotoxicity monitoring of Jurkat/L1236 cells in the presence/absence of Fibronectin: RTCA vs MTT assay.Notes: (A) We incubated Jurkat/L1236 cells (treatment point [+]) in the presence of trabectedin (T) (10 nM) + FasL (50 ng/mL), bendamustine (200 μM), oxaliplatin (100 μM), trabectedin (10 nM) + FasL (50 ng/mL), or bendamustine + oxaliplatin (up to 24 hours). (nih.gov)
  • tumor cells
  • It has previously been shown that Embelin inhibits proliferation, promotes apoptosis, and increases sensitivity and reduces resistance to chemotherapy drugs, in various types of tumor cells. (nih.gov)
  • It activates NF-κB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. (bpsbioscience.com)
  • The xCELLigence system is a new technological approach that allows the real-time cell analysis of adherent tumor cells. (nih.gov)
  • pathway
  • Effect of Embelin on the activity of the caspase pathway and effect of the caspase inhibitors, z-DEVD-fmk and Ac-LEHD-CHO, on Embelin-treated Jurkat cells. (nih.gov)
  • In the present study, we tested the hypothesis that rapid activation of the ERK pathway after PMA treatment protects Jurkat cells from MG-induced apoptosis. (aspetjournals.org)
  • compounds
  • In order to check if fibronectin allowed the real-time monitoring of the cytotoxic action of several compounds, we incubated Jurkat and L1236 cells with different drugs, such as FasL (50 ng/mL), oxaliplatin (100 μM), bendamustine (200 μM), or trabectedin (10 nM). (nih.gov)
  • treatments
  • This provides an excellent label-free tool for the screening of drug efficacy in nonadherent cells and discriminates optimal time points for further molecular analysis of cellular events associated with treatments, reducing both time and costs. (nih.gov)
  • optimal
  • To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. (thermofisher.com)
  • medium
  • Thaw Medium 2 (BPS Bioscience #60184) , 1 mg/ml of Geneticin (Life Technologies #11811031), and 150 μg/ml of Hygromycin B (Hyclone #SV30070.01) Cells should be grown at 37 o C with 5% CO 2 using complete growth medium (Thaw Medium 2, Geneticin, and Hygromycin B). (bpsbioscience.com)
  • The abundance of 3-methylcrotonyl-CoA carboxylase mRNA was lower in cells in medium D than cells in media N and S. The enrichment of biotinylated histones was higher at the SMVT promoter 1 in HepG2 and Jurkat cells in medium S compared with the corresponding cells in media D and N, presumably repressing the SMVT gene. (biomedsearch.com)