Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
Recombinant11
What is a Recombinant Antibody and Why is it Important? (cellsignal.com)
As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.com)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
Protein3
Low expression of CDK6 protein in ZR-75-1, Neuro-2a, and INS-1 cells is consistent with the predicted expression pattern. (cellsignal.com)
Immunoprecipitation of CDK6 protein from Jurkat cell extracts. (cellsignal.com)
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. (abcam.cn)
Validation1
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
Cell lines1
Western blot analysis of extracts from various cell lines using CDK6 (E3E3Q) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
Guarantee1
By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust. (cellsignal.com)
Human2
Immunohistochemical analysis of paraffin-embedded normal human colon (two fields of view) using CDK6 (E3E3Q) Rabbit mAb (left) or a CDK6 Rabbit mAb (right). (cellsignal.com)