Journal of cell biology cell biology clone cells. Medical search. Frequent questions

At just 31 years old, Hochedlinger has already worked on therapeutic cloning in a mouse model ( 2 ), reprogramming cancer nuclei ( 3 ), and the molecular mechanisms controlling stem cell pluripotency ( 4 ). (rupress.org)
When investigating the causes of this failure via cellular and molecular analysis of 2-cell zygotes and the successive cell divisions (blastomeres), all kinds of abnormalities were found. (sibi.org)
Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. (deepdyve.com)
2013) The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins. (scirp.org)
Therefore, it is important to explore candidate genes that could have significance to the clinical diagnosis, as well as the treatment of esophageal squamous cell carcinoma (ESCC) at the molecular level [ 4 ]. (hindawi.com)
Molecular Biology of the Channel Catfish Gonadotropin Receptors: 2. (biolreprod.org)
Molecular cloning and transcriptional mapping of the mouse cellular retinoic acid-binding protein gene. (ulaval.ca)
Dr. He is a basic science researcher by training in the fields of biochemistry and molecular biology and has been conducting clinical research for the past 20 years at Nemours. (nemours.org)
Interest in an undergraduate biochemistry course led me to a Ph.D. program in Biochemistry and Molecular Biology where I researched transcriptional regulation of type I collagen gene in cells from hypertensive fibrotic animal models. (fdu.edu)
My central interest is molecular cell biology. (fdu.edu)
I adopted a techniques course from the chemistry program and made it into a molecular biology techniques course, created a biotechniques course, and involved my NYU mentor in creating a bioinformatics course. (fdu.edu)
The molecular mechanisms potentially responsible for cadmium -induced cell transformation and tumorigenesis were investigated using Balb/c-3T3 cells transformed with cadmium chloride. (cdc.gov)
When the iv mouse mutation was cloned, it was found to encode a molecular motor protein, an axonemal dynein, and was named lrd , for left-right dynein (human homolog is DNAH11 / DNAHC11 ). (medscape.com)

Komatsu, T., Moriya, N. and Shiohara, T. (1996) T cell receptor (TCR) repertoire and function of human epidermal T cells: Restricted TCR V alpha-V beta genes are utilized by T cells residing in the lesional epidermis in fixed drug eruption. (scirp.org)
This research led me to post-doctoral fellowships predominantly on the extracellular matrix genes methylation, transcription, cloning, and photomedicine at Boston University Medical Center, Massachusetts General Hospital, and NYU Medical Center. (fdu.edu)
B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)
A chimera instead contains discrete cell populations with two unique sets of parental genes. (asu.edu)
The approach was applied to five different hybridomas producing human monoclonal antibodies and variable regions for both bold gamma and mu heavy chain and kappa and lambda light chain genes were successfully cloned. (lu.se)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

This also might have explained why cloning is inefficient: only 1-3% of cloned embryos eventually develop into an adult clone. (rupress.org)
True cloning performed by nuclear transfer from an adult and differentiated somatic cell to a previously enucleated egg (somatic cell nuclear transfer, SCNT), gives rise to a new cell, the nuclovulo (nucleus+ovum), distinct from the zygote because the sperm is not involved in its creation, while both can develop as embryos and give rise to offspring. (sibi.org)
The efﬁciency of cloning, deﬁned as the proportion of transferred embryos that result in viable offspring, is approximately 2 to 3% for all species. (sibi.org)
To educate its citizens about research into chimeras made from human and non-human animal cells, the United Kingdom's Human Fertilisation Embryology Authority published the consultation piece Hybrids and Chimeras: A Consultation on the Ethical and Social Implications of Creating Human/Animal Embryos in Research, in 2007. (asu.edu)
When two embryos are correctly joined before the 32-cell stage, the embryo will develop normally and exhibit a mosaic pattern of cells as an adult. (asu.edu)

Transferring a terminally differentiated cell nucleus into an egg cell that has had its own nucleus removed wipes away the epigenetic marks of differentiation and allows the nucleus once again to code for any cell type. (rupress.org)
Thereafter, dermomyotome-derived mpc clones rapidly expand while some progeny undergo terminal differentiation, leading to stochastic clonal drift within the mpc pool. (ox.ac.uk)
Despite different origin, all pluripotent lines demonstrate considerable similarity of the major biological properties: active self-renewal and differentiation into various somatic and germ cells in vitro and in vivo, similar gene expression profiles, and similar cell cycle structure. (elsevierpure.com)
Cell-cell interactions promote mammary epithelial cell differentiation. (silverchair.com)
To investigate cell-cell interactions that may influence mammary epithelial cell differentiation, we developed a co-culture system of murine mammary epithelium and adipocytes and other fibroblasts. (silverchair.com)
Swiss 3T3 cells, newborn foreskin fibroblasts, substrate-attached material ("extracellular matrix"), and tissue culture plastic did not support casein synthesis, whereas monolayers of 3T3-L1 and 3T3-C2 cells, a subclone of Swiss 3T3 cells that does not undergo adipocyte differentiation, did. (silverchair.com)
We conclude that interaction between mammary epithelium and other specific nonepithelial cells markedly influences the acquisition of hormone sensitivity of the epithelium and hormone-dependent differentiation. (silverchair.com)
Mammalian cells produce PGs, which are involved in biological processes such as cell growth and differentiation. (lu.se)

A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells. (wikipedia.org)
These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. (scirp.org)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
Combined with simple proliferative responses, the identified nucleotides are also shown to have functional consequences by inducing T cell proliferation for CD4/V β 20-1 + T cells, while failing to induce proliferation in other T cell isolates. (scirp.org)
SCARA5 promoted cell proliferation and migration but inhibited apoptosis in ESCC. (hindawi.com)
SCARA5 expression is significantly downregulated in various tumor tissues including liver cancer (LC), rectal cancer, and glioblastoma [ 15 , 16 ] and might affect cell proliferation and invasion abilities by the focal adhesion kinase (FAK) signaling pathway and epithelial-mesenchymal transition (EMT) [ 5 , 17 - 20 ]. (hindawi.com)
An accompanying proliferation of erythroid cells and megakaryocytes usually is present. (medscape.com)

It was thought that mammalian cells might be refractory to cloning. (rupress.org)
Aim: We previously reported changes of DNA methylation and transcription patterns in mammalian cells that carry integrated foreign DNA. (uni-koeln.de)
Here, we establish a platform for forward genetic screening using CRISPR/Cas9 to engineer dMMR into mammalian cells. (elsevierpure.com)
In each screen, compound-resistant alleles emerged in drug-resistant clones, supporting the notion that engineered dMMR enables forward genetic screening in mammalian cells. (elsevierpure.com)
Identification of binding sites of EVI1 in mammalian cells. (rochester.edu)

Whether this trick was even possible was a fundamental question of developmental biology until Konrad Hochedlinger, as a Ph.D. student, finally delivered unequivocal proof ( 1 ). (rupress.org)
Russian Journal of Developmental Biology , 39 (6), 325-336. (elsevierpure.com)
Gordeeva, OF & Mitalipov, SM 2008, ' Pluripotent stem cells: Maintenance of genetic and epigenetic stability and prospects of cell technologies ', Russian Journal of Developmental Biology , vol. 39, no. 6, pp. 325-336. (elsevierpure.com)

Ten years of intense studies on the stability of different human and monkey embryonic stem cells demonstrated that, irrespective of their origin, long-term in vitro cultures lead to the accumulation of chromosomal and gene mutations as well as epigenetic changes that can cause oncogenic transformation of cells. (elsevierpure.com)
Therapeutic cloning, which creates embryonic stem cells . (medlineplus.gov)

Methods: Differential transcription and CpG methylation patterns were compared between transgenomic and nontransgenomic cell clones by using gene chip microarray systems. (uni-koeln.de)

Results: In 4.7% of the 28.869 gene segments analyzed, transcriptional activities were up-or downregulated in the transgenomic cell clones. (uni-koeln.de)
Stem cell-like transcriptional reprogramming mediates metastatic resistance to mTOR inhibition. (rochester.edu)

Among the factors thought to contribute to the greater success in cloning cattle are the relatively late embryonic genome activation speciﬁc for this species [16 -18] and the optimization of reproductive technologies, such as in vitro embryo production and embryo transfer, brought about by the cattle industry [19]. (sibi.org)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
Engler, O.B., Strasser, I., Naisbitt, D.J., Cerny, A. and Pichler, W.J. (2004) A chemically inert drug can stimulate T cells in vitro by their T cell receptor in non-sensitised individuals. (scirp.org)
Shortly after TPMV was isolated, in vitro studies involving primary cultures of guinea pig embryonic kidney, lung, and heart cells supported replication of this virus ( 13 ). (cdc.gov)
A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
We demonstrate the utility of this approach to identify mechanisms of drug action in mouse and human cancer cell lines using in vitro selections against three cellular toxins. (elsevierpure.com)
This review summarizes the research data on the genetic and epigenetic stability of different lines of pluripotent stem cells after long-term in vitro culture. (elsevierpure.com)
The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)

Synthesis and accumulation of alpha-, beta-, and gamma-mouse casein within the epithelium was shown by immunohistochemical staining of cultured cells with anti-casein monoclonal antibodies, and the specificity of the immunohistochemical reaction was demonstrated using immunoblots. (silverchair.com)

2007) Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1. (scirp.org)
There was evidence of specificity in the cell-cell interaction that mediates hormone-dependent casein synthesis. (silverchair.com)

Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combina tion or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). (scirp.org)
After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. (scirp.org)
These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. (scirp.org)
In summary, cell viability was decreased and apoptosis (initial, mid and late) was in creased after hCG and/or Ang-(1 - 7) peptides treatments. (scirp.org)
Students of apoptosis and other types of cell death hypothesize that virtually all cells are genetically programmed to self-destruct. (nhbs.com)
S.W. Sherwood and R.T. Schimke, Cell Cycle Analysis of Apoptosis Using Flow Cytometry. (nhbs.com)

Differential display analysis of gene expression revealed consistent and reproducible overexpression of a transcript in the transformed cells compared with the nontransformed cells. (cdc.gov)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

Its pharmacology and cell biology are largely unknown, and no known natural cell lines or tissues possess sufficient numbers of fBB 4 -R's to allow either of these to be determined. (acs.org)
However, stable fBB 4 -R cell lines were obtained in CHO-K1 cells which were shown to faithfully demonstrate the correct pharmacology of the related Bn receptor, the GRP receptor, when expressed in these cells. (acs.org)

Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan. (ox.ac.uk)
It is shown that, after initial formation of the primary myotome, at least 15 muscle precursor cells (mpcs) seed each somite, where they proliferate but contribute little to muscle growth prior to hatching. (ox.ac.uk)
Acute lymphocytic leukemia (ALL) is a malignant clonal disorder of the bone marrow lymphopoietic precursor cells. (medscape.com)
Acute myelogenous leukemia (AML) is a group of neoplastic disorders of the hematopoietic precursor cells of the bone marrow. (medscape.com)

Open Journal of Immunology , 3 , 165-174. (scirp.org)
Open Journal of Immunology, 3, in press. (scirp.org)
Journal of Allergy and Clinical Immunology, 128, 1266-1276. (scirp.org)
A genome-wide association study to identify genetic determinants of atopy in subjects from the United Kingdom Journal of Allergy and Clinical Immunology. (nottingham.ac.uk)

Wucherpfennig, K.W. and Hafler, D.A. (1995) A review of T-cell receptors in multiple sclerosis: Clonal expansion and persistence of human T-cells specific for an immunodominant myelin basic protein peptide. (scirp.org)
No evidence of cell-lineage-based clonal fate diversity was obtained. (ox.ac.uk)
However, in earlier studies, clonal analysis was restricted by the lack of a cell marker, present at all times, that makes a distinction between the two parental cell types in situ. (asu.edu)
This approach will permit analysis of B cell clonal ontogeny, antibody diversity and lymphoma cell progression and heterogeneity. (lu.se)

It is frequently used in cancer research laboratories to determine the effect of drugs or radiation on proliferating tumor cells as well as for titration of Cell-killing Particles (CKPs) in virus stocks. (wikipedia.org)
Although this technique can provide accurate results, the assay is time-consuming to set up and analyze and can only provide data on tumor cells that can grow in culture. (wikipedia.org)
Any type of cell could be used in an experiment, but since the goal of these experiments in oncological research is the discovery of more effective cancer treatments, human tumor cells are a typical choice. (wikipedia.org)
Both the structure of the PGs and the GAG chains, and the expression of the enzymes involved in their biosynthesis and degradation, vary between normal cells and tumor cells. (lu.se)

Watkins, S.L. and Pichler, W.J. (2013) Activating interactions of sulfanilamides with T cell receptors. (scirp.org)
2004) T cell receptors recognizing type II collagen in HLA-DR-transgenic mice characterized by highly restricted V beta usage. (scirp.org)

They used adult cells, but it was possible that the cells that gave rise to successfully cloned animals were derived from rare adult stem cells. (rupress.org)

Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. (neobiotechnologies.com)
fBB 4 -R expression in cells widely used for other Bn receptor subtypes was unsuccessful as was expression in two frog cell lines. (acs.org)
These phenotypes were similar to those induced by RhoA(G14V) in parental cells, and could be abolished by expression of RhoA(T19N), Rac1(G12V), or p190RhoGAP in the mutant-expressing cells. (silverchair.com)
Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. (nature.com)
A 31-kDa protein was detected in eukaryotic cells transfected with an expression vector containing the TEF-1delta cDNA. (cdc.gov)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

Mammary epithelium from mid-pregnant mice was plated on confluent irradiated monolayers of 3T3-L1 cells, a subclone of the Swiss 3T3 cell line that differentiates into adipocytes in monolayer culture and other cell monolayers (3T3-C2 cells, Swiss 3T3 cells, and human foreskin fibroblasts). (silverchair.com)
Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)

Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila. (lu.se)
Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). (lu.se)
In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F-actin organization is abnormal. (lu.se)

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. (neobiotechnologies.com)

PR-domain-containing Mds1-Evi1 is critical for long-term hematopoietic stem cell function. (rochester.edu)

Myoglobin-reactive T cell clones. (uthscsa.edu)

Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
2000. A metabolite of methoxychlor, 2,2-bis( p hydroxyphenyl)-1,1,1-trichloroethane, reduces testosterone biosynthesis in rat leydig cells through suppression of steady-state messenger ribonucleic acid levels of the cholesterol side-chain cleavage enzyme. (cdc.gov)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

In this review, we consider what is currently known of the genetic landscape of the commonest subtypes of renal cell cancer (RCC). (springer.com)
To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbed Musclebow2, was derived by enhancer trapping in zebrafish. (ox.ac.uk)
Cloning describes the processes used to create an exact genetic replica of another cell, tissue or organism. (medlineplus.gov)
The copied material, which has the same genetic makeup as the original, is referred to as a clone. (medlineplus.gov)

The time for 1 cell division is prolonged with respect to that of normal bone marrow blast cells. (medscape.com)
The bone marrow is gradually replaced by blast cells. (medscape.com)

In Silico Biology is a scientific research journal for the advancement of computational models and simulations applied to complex biological phenomena. (iospress.com)
Twenty years have passed since Dolly the sheep was born by cloning (somatic cell nuclear transfer, SCNT) but the results of non-human mammalian cloning are very poor, and cause animal diseases and huge biological losses. (sibi.org)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
Registry of Standard Biological Parts and the Synthetic Biology Engineering Research Center Registry of Parts 1 ), repositories for distributing biological materials (e.g. (nature.com)
Microfluidics enables single-cell manipulation and analysis, reaction volumes of nano- or picoliters (thereby reducing costs), high-throughput execution of parallel experiments, automated routine liquid handling, integration of multiple biological processes in a single system, and programmability for complex protocols. (nature.com)
The Journal of biological chemistry. (rochester.edu)
1974. Biological handbooks: Biology data book. (cdc.gov)

cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

The cells are "plated" in a tissue culture vessel and allowed to grow. (wikipedia.org)
Studies published in In Silico Biology generally use theoretical models and computational analysis to gain quantitative insights into regulatory processes and networks, cell physiology and morphology, tissue dynamics and organ systems. (iospress.com)
Novel aspects of T cells containing TCRV β 20-1 are numerous, ranging from pathogen specific reactivity to specific tissue homing, or possible T cell subsets. (scirp.org)
In adulthood, early-marked mpc clones label stable blocks of tissue comprising a significant fraction of either epaxial or hypaxial somite. (ox.ac.uk)
To prioritise pathways we will undertake high-throughput functional genomic screens using CRISPR, perform lung digital spatial transcriptomic profiling and utilise informative cell and tissue models. (nottingham.ac.uk)
This will inform in-depth mechanistic assays at the cell, tissue and organ scales to identify the key mechanisms underpinning regulation of lung function in health and disease. (nottingham.ac.uk)
Researchers hope to use these cells to grow healthy tissue to replace injured or diseased tissues in the human body. (medlineplus.gov)

Permanent lines of pluripotent stem cells can be obtained from humans and monkeys using different techniques and from different sources-inner cell mass of the blastocyst, primary germ cells, parthenogenetic oocytes, and mature spermatogonia-as well as by transgenic modification of various adult somatic cells. (elsevierpure.com)

For Cell-killing Particle assays, the surviving fraction of cells is used to approximate the Poisson Distribution of virus particles amongst cells and therefore determine the number of CKPs encountered by each cell. (wikipedia.org)
Merging computational and simple cell assays, this work establishes a role of nucleotides in T cells found to contain this TCR subtype. (scirp.org)

Blood cells, molecules & diseases. (rochester.edu)

In a meeting in Washington (3 December 2001) the researcher Tanja Dominko presented the results of monkey cloning (Macacus rhesus) when she worked at the Regional Center of Research in Primates of Beaverton, Oregon (USA). (sibi.org)
P. M. Frossard, M. J. Malloy, G. G. Lestringant and J. P. Kane, "Haplotypes of the Human Renin Gene Associated with Essential Hypertension and Stroke," Journal of Human Hypertension, Vol. 15, No. 1, 2001, pp. 49-55. (scirp.org)

So far the reprogramming of somatic cells shows very low rates of efficiency (~0.0006-1%) that have not improved in the last two decades of continuous research. (sibi.org)

Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)

Why Cloning in Non-Human Mammalians Fail? (sibi.org)
1998) Comparative sequence analysis of the human T cell receptor beta chain in juvenile rheumatoid arthritis and juvenile spondylarthropathies: Evidence for antigenic selection of T cells in the synovium. (scirp.org)
Conclusion: The data document genome-wide effects of foreign DNA insertions on the epigenetic stability of human cells. (uni-koeln.de)
Evaluation of differentiated human bronchial epithelial cell culture systems for asthma research Journal of Allergy. (nottingham.ac.uk)
In 2007, Françoise Baylis and Jason Scott Robert published "Part-Human Chimeras: Worrying the Facts, Probing the Ethics" in The American Journal of Bioethics. (asu.edu)
Within their article, hereafter "Part-Human Chimeras," the authors offer corrections on "Thinking About the Human Neuron Mouse," a report published in The American Journal of Bioethics in 2007 by Henry Greely, Mildred K. Cho, Linda F. Hogle, and Debra M. Satz, which discussed the debate on the ethics of creating part-human chimeras. (asu.edu)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

The reason I then was drawn into epigenetics and stem cell biology was a lecture at the IMP by Rudolf Jaenisch. (rupress.org)

Angiotensin II Activates Extracellular Signal Regulated Kinases via Protein Kinase C and Epidermal Growth Factor Receptor in Breast Cancer Cells," Journal of Cellular Physiology, Vol. 196, No. 2, 2003, pp. 370-377. (scirp.org)

We found that mutation of Tyr31/118 caused enhanced activation of RhoA and premature formation of stress fibers with substantial loss of efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells. (silverchair.com)

Prior to SCNT, the somatic cell (differentiated) must be reprogramed to a similar state of a pluripotent embryonic cell (undifferentiated) before the nucleus is extracted and transferred. (sibi.org)
The prospects of using pluripotent stem cells of different origin in cell therapy and pharmacological studies were considered. (elsevierpure.com)

Many fields in experimental biology and medicine employ transgenomic or otherwise genome-manipulated cells or organisms without considering the epigenetic consequences for the recipient genomes. (uni-koeln.de)

A cDNA clone coding for a membrane proteoglycan core protein was isolated from a neonatal rat Schwann cell cDNA library by screening with an oligonucleotide based on a conserved sequence in cDNAs coding for previously described proteoglycan core proteins. (rupress.org)

P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford, Quantification of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer. (nhbs.com)

I asked whether a terminally differentiated cell is still amenable to reprogramming and able to give rise to a cloned animal. (rupress.org)

Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
AML is not a disorder of rapidly proliferating neoplastic cells. (medscape.com)

Cell Chemical Biology , 26 (9), 1315-1321.e3. (elsevierpure.com)

Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)

Although isolation of TPMV predates that of all other hantaviruses, including prototype HTNV, little is known about its biology and genetics. (cdc.gov)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

Hypocatalasia is the deficiency of an enzyme called catalase in red blood cells. (wisdompanel.com)
The catalase enzyme plays an important role in the cells defense against a type of chemical damage, known as oxidative damage. (wisdompanel.com)
cDNA cloning of mutant catalase in acatalasemic beagle dog: Single nucleotide substitution leading to thermal-instability and enhanced proteolysis of mutant enzyme. (wisdompanel.com)
To determine the structural requirements for beta 4GalT7 activity, we have cloned and expressed the enzyme and designed a focused library of 2-naphthyl beta-D-xylopyranosides with modifications of the xylose moiety. (lu.se)

Then in '97 the paper by Ian Wilmut came out on the cloning of Dolly the sheep. (rupress.org)
This question had not been resolved unequivocally by the cloning of Dolly or other mammals. (rupress.org)
The most famous clone was a Scottish sheep named Dolly. (medlineplus.gov)

One such automated machine works by accepting certain types of cell plates through a slot (not unlike a CD player), taking a photograph, and uploading it to a computer for immediate analysis. (wikipedia.org)
Journal of Clinical Laboratory Analysis. (wikipedia.org)
Regarding the cell cycle analysis, we did not observed any changes in cell cycle phases. (scirp.org)

Nuclear transfer is one of the most sophisticated, fickle, and challenging techniques in cell biology. (rupress.org)

Anatomy24

Organisms9

Diseases4

Chemicals and Drugs17

Analytical, Diagnostic and Therapeutic Techniques and Equipment43

Psychiatry and Psychology3

Phenomena and Processes51

Disciplines and Occupations34

Anthropology, Education, Sociology and Social Phenomena12

Technology, Industry, Agriculture11

Humanities7

Information Science58

Named Groups2

Health Care17

Geographicals2

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Genes7

Embryos5

Differentiation8

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Mammalian cells5

Developmental biology3

Embryonic stem2

METHYLATION1

Transcriptional2

Vitro9

Monoclonal1

Specificity2

Apoptosis8

Gene expression2

Pharmacology2

Precursor cells4

Immunology4

Clonal4

Tumor cells4

Receptors2

Successfully1

Expression6

Fibroblasts2

Oocyte3

Epithelial cells1

Hematopoietic stem1

Reactive1

20004

Genetic4

Bone marrow2

Biological8

Antibodies1

Tissue7

Adult1

Assays2

Molecules1

20012

Somatic cells1

Granulosa cells2

Human8

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Physiology1

Mutation1

Pluripotent2

GENOMES1

CDNA library1

Flow cytometer1

Terminally differentiated1

Rapidly2

Chemical biology1

Abstract1

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Enzyme4

Dolly3

Analysis3

Nuclear transfer1