As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.com)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
Western blot analysis of HepG2 cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. (neobiotechnologies.com)
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. (neobiotechnologies.com)
Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. (enquirebio.com)
This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (genetex.com)
Protein4
Low expression of CDK6 protein in ZR-75-1, Neuro-2a, and INS-1 cells is consistent with the predicted expression pattern. (cellsignal.com)
Immunoprecipitation of CDK6 protein from Jurkat cell extracts. (cellsignal.com)
Tubulin was used as the cytoplasmic control, and lamin A/C was used as the nuclear protein-loading control. (bms-911543.com)
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. (abcam.cn)
Rabbit9
Western blot analysis of extracts from various cell lines using CDK6 (E3E3Q) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
using CDK6 (E3E3Q) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
Western blot analysis was performed using CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded normal human colon (two fields of view) using CDK6 (E3E3Q) Rabbit mAb (left) or a CDK6 Rabbit mAb (right). (cellsignal.com)
Formalin-fixed, paraffin-embedded human cervix stained with P16INK4aRecombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (cusabio.com)
Description: A monoclonal antibody for detection of IgG Fc specific Heavy Chain) from Rabbit. (sebov-detection.com)
Polyclonal2
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
We also identified a rare class of ZIKV-cross-reactive human monoclonal antibodies with increased somatic hypermutation and broad neutralization against multiple flaviviruses. (bvsalud.org)
Description: A Monoclonal antibody against Human human IgG(Fc Specific). (sebov-detection.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Casein Kinase 1 Delta (CSNK1d) in Tissue homogenates and other biological fluids. (1elisakits.com)
Assay1
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
Specificity1
The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. (cellsignal.com)
Detection1
B) Luciferase reporter gene detection in ZR75-1 and MDA-MB-231 breast cancer cells co-transfected with expression in ZR75-1 and MDA-MB-231 cells, which were transfected with ZKSCAN5 shRNA, control shRNA, or ZKSCAN5 shRNA plus shRNA-resistant ZKSCAN5 (ZKSCAN5-R). The representative Western blot further showed the expression of ZKSCAN5. (bms-911543.com)
Host1
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
Mouse1
Description: Mouse Monoclonal Prostate Specific Antigen (PSA) Antibody. (sebov-detection.com)
Regulatory1
The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. (cusabio.com)
By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust. (cellsignal.com)
This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial. (enquirebio.com)
Primary1
Anti-ZKSCAN5 (SAB4501021), anti-VEGFC (ab83905), and anti-LYVE1 (ab10278) primary antibodies were used at 1:100, 1:100, and 1:50 dilutions, respectively. (bms-911543.com)
Support1
in situations where an antibody will be used to support large, long-term studies, this can be an especially critical factor. (cellsignal.com)
Similar1
Data shown are the mean SD of triplicate measurements from experiments that have been repeated three times with similar results (B, C). **p 0.01 versus control shRNA. (bms-911543.com)
Multiple1
Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. (enquirebio.com)
Group1
p 0.01 versus the control shRNA group (A, B). (C, D) Wound healing assays for HLECs cultured in conditioned medium from ZR75-1 or MDA-MB-231 cells, which were Glutaminase-IN-1 stably infected as in (A). The image shown is one of the representative results (C, D). Scale bar: 100 m. (bms-911543.com)
Expression1
In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
Properties1
The purpose of this study was to evaluate the controlled-release properties of nutrients from coconut water-based hydrogel. (lotusbiotechnologies.com)