For example, the medium can contain arginine labeled with six carbon-13 atoms (13C) instead of the normal carbon-12 (12C). (wikipedia.org)
A SILAC approach involving incorporation of tyrosine labeled with nine carbon-13 atoms (13C) instead of the normal carbon-12 (12C) has been utilized to study tyrosine kinase substrates in signaling pathways. (wikipedia.org)
The methionine at position 5 is oxidised and the leucine residue at position 8 is isotopically labelled with carbon-13(6) and nitrogen-15(1). (bestbiochem.com)
Below lists is the most common stable isotopically labeled amino acids that can be used for the synthesis of peptides with stable isotopes containing carbon 13 ( 13 C), Nitrogen 15 ( 15 N). (novoprolabs.com)
Specific stable isotope1
Stable isotope labeled peptides, or SIL peptides, maintain identical amino acid sequence with the natural ones released from the parent proteins by chemical or enzymatic cleavage, but some of the amino acid residuals are labeled by specific stable isotope, such as 2 H, 13 C, and/or 15 N atom(s). (novoprolabs.com)
Substrates2
Both liquid and gas chromatography coupled to mass spectrometry (LC-MS and GC-MS, respectively) have been used in targeted and untargeted experiments but require the use of isotopically labeled substrates to glean information on the dynamic processes of DCs. (chromatographyonline.com)
We used a dedicated N-terminomics technique, iTRAQ terminal amine isotopic labeling of substrates (TAILS), to characterize the human platelet N-terminome, proteome, and posttranslational modifications throughout platelet storage over 9 days under blood-banking conditions. (ashpublications.org)
Isotopic1
Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. (wikipedia.org)
Peptides3
The labeled peptides co-elute with their nonlabeled counterparts and are distinguishable by mass. (cdc.gov)
Stable isotope labeled peptides have been widely applied in the nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS). The combination of SIL peptides with NMR spectroscopy allow for the incorporation of NMR active nuclei which can reduce the complexity of spectra, and then help researchers to obtain novel correlations between atoms for more structural information. (novoprolabs.com)
With more than 10 years of experience, and as a world-leading peptide manufacturing organization, NovoPro can provide customized peptides labeled with stable isotopes including 2 H, 15 N & 13 C, respectively, or a combination of 15 N & 13 C. Even when the peptides are attached with specific modification motifs. (novoprolabs.com)
Isotopes2
In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
have described a urea dilution method using the triple labelled [l3Cl5N2] urea isotope and gas chromatography-MS (GC-MS) analysis for study- Keywords: gas chromatography-isotope ratio MS, stable isotopes , ureamia, urea dilution. (silverchair.com)
Amino acid1
The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. (wikipedia.org)
Quantification1
Isotopically labeled standards are added (traces not shown) as internal standards for quantification. (cdc.gov)
Stable2
Nicolaas E.P. Deutz In 1982 and 2011, Clinical Science published papers that used infusion of stable isotope -labeled amino acids to assess skeletal muscle protein synthesis in the fasted and fed state and before and after a period of increased intake of omega-3 fatty acids. (silverchair.com)
M. J. RENNIE We used stable - isotope -labelled amino acids to measure the effects of alcoholic liver disease (ALD) on whole-body protein turnover and small-intestinal mucosal protein synthesis. (silverchair.com)
Technique2
Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4). (wikipedia.org)
Isotope-labeling is a useful technique for understanding cellular metabolism. (mdpi.com)
Method1
Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
The scheme relies on the customized labeling of Isoleucine, Leucine and Valine, using a -ketobutyrate or a -ketoisovalerate respectively as labeled precursors (Figure below), that are used to probe long distances restraints. (cortecnet.com)
The motivation for this work is to develop a useful doubly labeled species for subsequent nD NMR studies of proteins, whose isoleucine residues have been fluorinated. (biorxiv.org)
Valine1
As an intermediate for valine and leucine biosynthesis, 2-ketoisovalerate is decarboxylated by a heterologously expressed broad-substrate-range α-ketoisovalerate decarboxylase (Kivd) to isobutyaldehyde, and subsequently reduced into IB by an alcohol dehydrogenase (Adh). (biomedcentral.com)
Stable isotope1
Cortec Isotope also offers custom synthesis of stable isotope labeled a -ketoacids ( 13 C, 2 H, 17 O) that best meet your needs. (cortecnet.com)
Incorporation3
A SILAC approach involving incorporation of tyrosine labeled with nine carbon-13 atoms (13C) instead of the normal carbon-12 (12C) has been utilized to study tyrosine kinase substrates in signaling pathways. (wikipedia.org)
Such a partially degraded RNA preparation, but not the native ma- terial, is an excellent primer for the incorporation of C-labeled adenosine phosphate and C**-labeled cytidine phosphate from their respective triphosphates by an enzyme obtained from Escherichia colt. (nih.gov)
The selective incorporation of amino acids with 1 H- 13 C labeled methyl group into a fully deuterated protein is interesting since methyl groups are over-represented in protein hydrophobic cores. (cortecnet.com)
Proteins6
T he ILV labeling scheme allows the production of uniformly D, 13 C and 15 N labeled proteins using 13 C 6 ,D 7 Glucose and 15 NH 4 Cl as respectively carbon and nitrogen sources. (cortecnet.com)
Thus, whereas the methyl group of Methionine can be easily labeled into deuterated proteins, additional considerations need to be taken into account for amino acids such as Alanine and Threonine in order to suppress scrambling. (cortecnet.com)
The 13 CF 3 group is a promising label for heteronuclear ( 19 F, 13 C) NMR studies of proteins. (biorxiv.org)
An alternative approach to 19 F labeling of proteins is to make use of a thiol specific fluorinated probe, which frequently consists of a terminal trifluoromethyl group 4 . (biorxiv.org)
For very large proteins, spectral overlap may become problematic for biosynthetically labeled proteins. (biorxiv.org)
Uniformly labeled CTP-C™ was prepared as follows. (nih.gov)
Methyl groups1
Desirable locations for this NMR spin label include the branched chain amino acid methyl groups. (biorxiv.org)
Compounds2
This technology relates to the field of radioactive, isotopically-labeled calcofluor derivatives and uses of such compounds to detect a broad spectrum of filamentous fungi including pathogenic species such as Aspergillus and Mucorales , by diagnostic imaging methods such as positron emission tomography (PET) scanning. (nih.gov)
For isotopically labeled compounds, MW listed is for the fully enriched product. (isotope.com)
Amino4
In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
The NeuCode amino acid method is similar to SILAC but differs in that the labeling only utilizes heavy amino acids. (wikipedia.org)
However, a doubly ( 13 C, 19 F) labeled amino acid should provide greater resolution in two-dimensional ( 19 F, 13 C) NMR spectra, with further possibilities of assignment without mutational analysis. (biorxiv.org)
Recently, a new technique called NeuCode (neutron encoding) SILAC, has augmented the level of multiplexing achievable with metabolic labeling (up to 4). (wikipedia.org)
Counterparts1
The labeled peptides co-elute with their nonlabeled counterparts and are distinguishable by mass. (cdc.gov)
Laboratories1
EXPERIMENTAL PROCEDURE Materials Preparation of Substrates-ATP-8-C' was purchased from Schwarz Laboratories and was judged to be 98% isotopically pure by paper electrophoresis. (nih.gov)
Grown1
Uni- formly labeled RNA-C" was isolated from Chromatium, strain D, grown with ClO, as sole source of carbon. (nih.gov)
Studies1
In human protein turnover studies with isotopically labeled leucine (Leu) as a tracer, plasma ketoisocaproate (KIC) enrichment is extensively used as a surrogate measure of intracellular leucine enrichment. (elsevierpure.com)
High1
Here, we tracked decomposition of isotopically labeled low and high melanin fungal necromass and measured 13C and 15N accumulation in surrounding soils and microbial communities over 77 d in a temperate forest in Minnesota, USA. (bvsalud.org)