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  • reaction
  • which contains the following subunits: * A) a reactive site for coupling the biologically active ligand, * B) a photoreactive site for reaction with the target protein, * C) a biotin label, which allows purification of the protein-ligand com- [*] Priv. (docme.ru)
  • Because the labeled atom has the same number of protons, it will behave in almost exactly the same way as its unlabeled counterpart and, with few exceptions, will not interfere with the reaction under investigation. (wikipedia.org)
  • Support for the reaction mechanism is based on isotope labeling. (wikipedia.org)
  • Yeast
  • The combination of 15 N/ 13 C stable isotope labelling (SIL) and LC-MS/MS revealed a total of 52 modifications in RNA from E. coli and yeast, including 10 previously undescribed modifications. (rsc.org)
  • ratios
  • Isotope tracers are commonly used in the form of isotope ratios. (wikipedia.org)
  • Because the different isotope ratios for the two kinds of plants propagate through the food chain, it is possible to determine if the principal diet of a human or other animal consists primarily of C3 plants or C4 plants by measuring the isotopic signature of their collagen and other tissues. (wikipedia.org)
  • protein
  • In photoaffinity labeling, a covalent linkage is created between a ligand and a protein upon irradiation by UV light. (docme.ru)
  • The requirements for the ideal photoaffinity label include its chemical stability prior to photoactivation, its easy photolysis at wavelengths that do not cause photochemical damage to the protein, and high reactivity of the intermediate product to C H groups and to nucleophilic X atoms from X H groups. (docme.ru)
  • naturally
  • Biomarkers with the heavier isotope incorporated into them can be separated from biomarkers containing the more naturally abundant lighter isotope by isopycnic centrifugation. (wikipedia.org)
  • atoms
  • The reactant is 'labeled' by replacing specific atoms by their isotope. (wikipedia.org)
  • In this technique, one or more of the atoms of the molecule of interest is substituted for an atom of the same chemical element, but of a different isotope (like a radioactive isotope used in radioactive tracing). (wikipedia.org)
  • NMR and MS detects isotopic differences, which allows information about the position of the labeled atoms in the products' structure to be determined. (wikipedia.org)
  • biotin label
  • If a cleavage site is also incorporated, the biotin label can be released subsequently. (docme.ru)
  • The different structural elements are indicated as A) reactive site 1 (gray circle), B) photoreactive site (gray square), C) biotin label (gray triangle), and D) isotope label (open circles). (docme.ru)
  • yields
  • Gossert AD, Hinniger A, Gutmann S, Jahnke W, Strauss A, Fernández C (2011) A simple protocol for amino acid type selective isotope labeling in insect cells with improved yields and high reproducibility. (springer.com)
  • affinity
  • If tri- or tetrafunctional photoaffinity labels containing a biotin group are used, the created products can be enriched by affinity purification on avidin beads. (docme.ru)
  • abundance
  • By studying the ratio between two isotopes of the same element, we avoid effects involving the overall abundance of the element, which usually swamp the much smaller variations in isotopic abundances. (wikipedia.org)
  • A radiogenic isotope tracer involves an isotope produced by radioactive decay, which is usually in a ratio with a non-radiogenic isotope (whose abundance in the earth does not vary due to radioactive decay). (wikipedia.org)
  • Cell Culture
  • Egorova-Zachernyuk TA, Bosman GJ, Pistorius AM, DeGrip WJ (2009) Production of yeastolates for uniform stable isotope labelling in eukaryotic cell culture. (springer.com)
  • We utilized the essential requirement of niacin and tryptophan for NAD synthesis in mammalian cells to devise a stable isotope labeling by essential nutrients in cell culture (SILEC) method for efficient labeling of intracellular NAD(H) and NADP(H) pools. (rsc.org)
  • mice
  • 2015 . Pulsed azidohomoalanine labeling in mammals (PALM) detects changes in liver-specific LKB1 knockout mice. (isotope.com)
  • Analysis
  • To improve analysis of this compound, CIL collaborated with researchers at North Carolina State University to offer a new 13 C 3 / 15 N 2 labeled BMAA standard. (isotope.com)
  • novel
  • A novel photoaffinity label 1 (Figure 1) has been presented recently by Lamos et al. (docme.ru)
  • subsequently
  • More recently, Ruoff and collaborators have demonstrated synthesis of large area monolayer graphene on copper foil by chemical vapor deposition, for which relatively high carrier mobilities have been obtained, and subsequently have used isotopic labeling and micro-Raman mapping to map grains and grain boundaries in such atom thick layers and to elucidate growth mechanisms, and studied their performance as transparent conductive electrodes. (wikipedia.org)
  • example
  • For example, when ethyl propionate with an oxygen-18 labeled ethoxy group is treated with sodium hydroxide (NaOH), the oxygen-18 is completely absent from the sodium propionate product and is found exclusively in the ethanol formed. (wikipedia.org)
  • Cells
  • Using a two-label system incorporating [ 13 C 3 15 N 1 ]-nicotinamide and [ 13 C 11 ]-tryptophan, we quantify the relative contribution of salvage and de novo NAD synthesis, respectively, in S. cerevisiae and HepG2 human hepatocellular carcinoma cells under basal conditions. (rsc.org)
  • Standards
  • Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). (indigo.ca)
  • antibodies
  • Because of its relatively long half-life and emission of low-energy photons which can be detected by gamma-counter crystal detectors, I-125 is a preferred isotope for tagging antibodies in radioimmunoassay and other gamma-counting procedures involving proteins outside the body. (wikipedia.org)
  • Zirconium-89 is employed in specialized diagnostic applications using positron emission tomography imaging, for example, with zirconium-89 labeled antibodies (immuno-PET). (wikipedia.org)
  • neutron
  • Its production follows the reaction: 124Xe (n,γ)→ 125mXe(57s)→125I (59,4 d) 124Xe (n,γ)→ 125gXe(19,9h)→125I (59,4 d) The irradiation target is natural xenon gas containing 0.0965% 124Xe, which is the target isotope for making I-125 by neutron capture. (wikipedia.org)
  • the other isotope with a significant neutron absorption cross section is 91Zr with a cross section of 1.24 barns. (wikipedia.org)
  • ratio
  • In addition, there are at least three non-local individuals with higher values, including one with an extremely isotope ratio that cannot be from the Harappa region. (blogspot.com)
  • However, the 2010 NASA press release also noted that: "One reason the chlorinated organics found by Viking were interpreted as contaminants from Earth was that the ratio of two isotopes of chlorine in them matched the three-to-one ratio for those isotopes on Earth. (wikipedia.org)
  • selectively
  • In organic chemistry, hydrogenation of a C-Cl bond with deuterium is used to selectively label the aromatic ring for use in experiments dealing with the kinetic isotope effect. (wikipedia.org)
  • The X-rays and gamma rays are of low enough energy to deliver a higher radiation dose selectively to nearby tissues, in "permanent" brachytherapy where the isotope capsules are left in place (I-125 competes with palladium-103 in such uses). (wikipedia.org)
  • cell
  • CFL1 Full-Length MS Protein Standard (NP_005498), Labeled with [U- 13C6, 15NL-Arginine and [U- 13C6, 15NL-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. (creative-proteomics.com)
  • The internal conversion and Auger electrons cause little damage outside the cell which contains the isotope atom. (wikipedia.org)
  • band
  • Founded in 1998, the band is a project by members of several bands within the Japanese progressive rock/zeuhl scene and has released albums on two US labels, John Zorn's Tzadik Records and Skin Graft Records as well as on the Japanese label Magaibutsu. (wikipedia.org)
  • Drummer Tatsuya Yoshida, owner of the Magaibutsu label, has founded the band Ruins and has been active in several other bands and collaborations, e.g. (wikipedia.org)
  • Jeg started the label in 2004 when his friend Brendan Kelly of The Lawrence Arms wanted a new label to release the EP for his new band The Falcon, it has since released material for bands such as The Lillingtons, Cobra Skulls, The Copyrights, Sundowner, Teenage Bottlerocket, and more. (wikipedia.org)
  • Tranquility Bass's touring bassist, Matt Lux, is also the bassist for Chicago-based band Isotope 217. (wikipedia.org)
  • record label
  • 1999: Korekyojin (Tzadik) 2004: Arabesque (Magaibutsu) 2005: Isotope (Tzadik) 2006: Jackson (Magaibutsu) 2009: Swan Dive (Magaibutsu) 2011: Doldrums (with Tsuboy Akihisa) (Magaibutsu) 2011: Tundra (Magaibutsu/Skin Graft) Korekyojinn discography at AllMusic Korekyojinn at the webpage of the Magaibutsu record label Tatsuya Yoshida at AllMusic Mitsuro Nasuno: Credits at AllMusic Mitsuro Nasuno at Discogs Natsuki Kido at AllMusic Westergaard, Sean. (wikipedia.org)
  • Red Scare Industries (sometimes referred to as Red Scare Records) is a punk rock record label. (wikipedia.org)
  • Gull was a British record label founded in 1974. (wikipedia.org)
  • artists
  • The label has also worked with non Red Scare artists on additional projects, including "Red Oktoberfest", an annual weekend music festival in Chicago which has featured bands such as The Lawrence Arms, American Steel, and Off With Their Heads, and the Swingin' Utters tribute album, Untitled 21: A Juvenile Tribute to the Swingin' Utters, which most notably featured the Dropkick Murphys. (wikipedia.org)
  • means
  • With the aid of strontium, carbon, and oxygen isotopes, it is now possible to develop more direct means for determining the presence of non-local people in both regions. (blogspot.com)
  • album
  • Kandel reportedly tried to have the album released on multiple labels, only to have each of them fold after he sent it to them. (wikipedia.org)