Isolation protein expression. Medical search. Frequent questions

Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). (wikipedia.org)
CALIXAR's membrane protein services include membrane protein expression , solubilization, purification & characterization among others. (calixar.com)
RNA & protein isolation -Purification of total RNA, or recovery of both protein and RNA from the same sample of pre-enriched exosomes, can be achieved using the Invitrogen Total Exosome RNA and Protein Isolation Kit . (thermofisher.com)
PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
We kindly request that you contact us first by phone, e-mail or video conference to discuss new expression and purification projects. (embl.org)
In case expression constructs and/or protocols are already present, you can immediately fill out our protein expression and purification request form and send the completed form back to us. (embl.org)
After we receive the sequence-verified expression constructs, we will first perform small scale expression and purification tests in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
Once suitable conditions have been identified, we proceed to large scale expression (1-6 liter per construct) and protein purification using a variety of chromatographic techniques. (embl.org)
After harvesting the V1 virus, we perform small scale expression and purification tests, in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)

A full structural and functional protein characterization was carried on and in addition, the enzyme was able to form diamond crystals which diffracted at 1.7 Å. (unina.it)
A biochemical approach highlighted protein features with a functional characterization methodology. (unina.it)
Dynabeads offers an array of exosome isolation products and tools for characterization and analysis of their RNA and protein content, as well as in vitro and in vivo tracing to help you further the understanding of these fascinating entities. (thermofisher.com)
Isolation and characterization of piscine osteonectin and downregulation of its expression by PTH-related protein. (ualg.pt)
requiring higher instruments for his or her isolation and molecular characterization. (aetox.com)
Isolation and characterization of Akhmeta virus from wild caught rodents ( Apodemus spp. (cdc.gov)

The following is a list of notable proteins that are produced from recombinant DNA, using biomolecular engineering. (wikipedia.org)
In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
A much larger number of recombinant proteins is used in the research laboratory. (wikipedia.org)
As a result, recombinant lysosomal proteins are usually produced in mammalian cells. (wikipedia.org)
Recent studies have shown that it may be possible to produce recombinant lysosomal proteins with microorganisms such as Escherichia coli and Saccharomyces cerevisiae. (wikipedia.org)
Recombinant lysosomal proteins are used for both research and medical applications, such as enzyme replacement therapy. (wikipedia.org)
NEB has a long history in recombinant protein expression and has developed a wide array of solutions for proteins that are difficult to express. (neb.com)
I will start by outlining the most common problems encountered during the expression of recombinant proteins in E. coli and specific solutions will be given for each problem. (neb.com)
The two most common problems during recombinant protein expression are low expression level and solubility of the target protein. (neb.com)
Starting from native material or recombinant systems, we succeed with all types of membrane proteins: GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins. (calixar.com)
After cloning and gene heterologous expression in E. coli, the recombinant protein was obtained as high level in soluble form and then purified to homogeneity. (unina.it)
Gene cloning and expression were followed by a production of the recombinant protein in a soluble form. (unina.it)
Here, we describe a new method (immuno-SILAC) for the absolute quantification of proteins in complex samples based on polyclonal antibodies and stable isotope-labeled recombinant protein fragments to allow affinity enrichment prior to mass spectrometry analysis and accurate quantification. (nih.gov)
The approach was further developed to also generate quantitative data via the addition of heavy isotope-labeled recombinant protein fragment standards prior to trypsin digestion. (nih.gov)
The results suggest that polyclonal antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to allow for rapid mass spectrometry analysis taking advantage of a substantial reduction in sample complexity. (nih.gov)
After we receive the sequence-verified expression constructs, we will first generate the recombinant baculoviruses. (embl.org)

We investigate the functional relevance of de novo missense variants, specifically whether they are likely to disrupt protein interactions, and nominate novel genes in risk for ASD through integrated genomic, transcriptomic, and proteomic analyses. (springer.com)
For genes encoding the disrupted interactions, we evaluate their expression patterns across developing brains and within specific cell types, using both bulk and inferred cell-type-specific brain transcriptomes. (springer.com)
Finally, we integrate protein interactions and cell-type-specific co-expression networks together with published association data to implicate novel genes in ASD risk in a cell-type-specific manner. (springer.com)
Genes encoding disrupted complementary interactors tend to be risk genes, and an interaction network built from these proteins is enriched for ASD proteins. (springer.com)
Consistent with other studies, genes identified by disrupted protein interactions are expressed early in development and in excitatory and inhibitory neuronal lineages. (springer.com)
Using inferred gene co-expression for three neuronal cell types-excitatory, inhibitory, and neural progenitor-we implicate several hundred genes in risk (FDR \(\le \hspace{0.17em}\) 0.05), ~ 60% novel, with characteristics of genuine ASD genes. (springer.com)
Using the newly released and larger ASC dataset, we confirm these observations and take them in several new directions: (1) By defining a set of genes encoding these disrupted protein interactors in ASD subjects and another for their siblings, we evaluate their expression patterns in developing brain from fetal to early postnatal development and within general cell types of brain tissue. (springer.com)
HE4 is one of more than 8900 different human genes represented on U95a GeneChips (Affymetrix, Santa Clara, CA, USA), which we used previously to develop a molecular classification of 175 human carcinomas based on patterns of gene expression. (nature.com)
We took advantage of the antibody resources publicly available from the Human Protein Atlas project covering more than 80% of all human protein-coding genes. (nih.gov)
We found that the more thermally tolerant population had greater plasticity of gene expression and had significantly more downregulated genes, which may provide more energy to repair damage associated with thermal stress and to maintain basic functions at these extreme temperatures. (frontiersin.org)
Microarray analyses revealed that top expression of a number of genes is related to PAX3/7-FOXO1 fusion standing. (aetox.com)

Ribosomal protein S6 kinases are activated through phosphorylation in response to a variety of HORMONES and INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS. (bvsalud.org)

This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
Furthermore, production of intracellular membrane vesicles in Escherichia coli can be triggered by expression of Acholeplasma laidlawii lipid glycosyltransferases [ 5 ]. (biomedcentral.com)

Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)
The inner fluid can contain cargo molecules such as nucleic acids or soluble proteins. (biomedcentral.com)

The tyrosinase‐induced polymerization of peptides offers facile access to artificial mussel foot protein analogues. (wikipedia.org)
These proteins can vary in size from small peptides to large membrane-bound enzymes, with many different biochemical properties that often require optimization before high scale expression. (calixar.com)
We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)

Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)
Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
Co-expression of P9 and P12 in a Pseudomonas host results in the formation of intracellular vesicles that are potential intermediates in the phi6 virion assembly pathway. (biomedcentral.com)
A family of protein serine/threonine kinases which act as intracellular signalling intermediates. (bvsalud.org)
[ 3 ] Protein hZIP4 transports zinc ions from the cell exterior or lumen of intracellular organelles into the cytoplasm, where it is available to other newly synthesized proteins. (medscape.com)

Following me will be Corinna Tuckey, who will describe the utility and advantages of the PURExpress in vitro protein synthesis kit. (neb.com)
NEB also offers solutions for expressing these types of proteins, either in vitro or in vivo. (neb.com)
Exosomes are small (30-150 nm) vesicles containing sophisticated RNA and protein cargo, that are constantly secreted by all cells in vitro and in vivo . (thermofisher.com)
Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)
However, tPTS has largely been conducted in vitro or restricted to bacterial expression systems, cell lysates, nuclear extracts, or selection protocols 8 , 13 - 15 . (biorxiv.org)
Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)

For mammalian cells, we can work with most standard mammalian expression vectors (e.g. based on the pcDNA backbone) or BacMam vectors. (embl.org)

CALIXAR's approach allows to preserve the original structure and function of membrane proteins (GPCRs, Ion Channels, Transporters, Receptors, Anchors and Viral Proteins) providing solutions for pharmaceutical industries, biotechnology companies and academic teams to develop conformational antibodies, formulate new vaccines, carry out Structure Based Drug Discovery and/or HTS assays. (calixar.com)

When expressing membrane proteins, researchers must assume from the start that the protein will be difficult to express in functional form. (neb.com)
As a solution NEB offers a tunable T7 expression strain for the production of membrane proteins and other difficult targets so that the level of functional protein may be optimized. (neb.com)
CALIXAR develops and implements full-length membrane proteins while maintaining their structural and functional integrity. (calixar.com)
Proteins as the functional molecules in the cells are the main effectors of normal cellular and disease processes. (cns.org)
This result together with some common structural features led to the suggestive hypothesis of a possible functional link between mammalian PrP and Sho proteins. (nih.gov)
A protocol based on the simultaneous multiplex capture of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide detected in the subsequent mass spectrometry analysis. (nih.gov)

Used Western Blotting to check protein phosphorylation of different cell signaling pathways. (livecareer.com)
Phosphorylation of RIBOSOMAL PROTEIN S6 by enzymes in this class results in increased expression of 5' top MRNAs. (bvsalud.org)

NEW YORK (GenomeWeb) - Isolation of bacteria within different regions of the lungs of cystic fibrosis patients drives their diversification, according to a phylogenetic study appearing in Cell Host & Microbe today . (genomeweb.com)
Of these isolates, 83% were community produce plasmid-mediated -lactamases such as CMY-2, onset, and urine was the principal site of isolation (90% of which are derived from bacteria with chromosomally patients). (cdc.gov)

And then Mehmet Berkman will highlight the SHuffle line of E. coli strains for producing proteins with complex disulphide bonds. (neb.com)
However, expression problems are most often due to open reading frames with non-optimal codons for E. coli. (neb.com)
A gene can be ordered, it is optimized for E. coli expression and it can be at your lab within three weeks. (neb.com)
For expression in E. coli , we accept all commonly used expression vectors. (embl.org)
Our results demonstrate that the phi6 major envelope protein P9 can trigger formation of cytoplasmic membrane structures in E. coli in the absence of any other viral protein. (biomedcentral.com)

whereas in the cells isolated from high grade tumors, five isoforms of vimentin, transgelin, Mn-SOD, pyruvate kinase (PKM), GTP-binding nuclear protein Ran and 40S ribosomal protein SA were detected to be overexpressed. (cns.org)
Although specific for RIBOSOMAL PROTEIN S6 members of this class of kinases can act on a number of substrates within the cell. (bvsalud.org)
The immunosuppressant SIROLIMUS inhibits the activation of ribosomal protein S6 kinases. (bvsalud.org)

Hepatic stellate cells (HSCs), which are the major cells responsible for the formation of extracellular matrix (ECM) proteins during cirrhosis, are found in the space of Disse and act as a major storage site for vitamin A [ 14 , 15 ]. (biomedcentral.com)
The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. (cancerindex.org)
It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). (cancerindex.org)
SPRN comprises two exons, with the open reading frame (ORF) contained within exon 2, and codes for a protein of 130-150 amino acids named Shadoo (Japanese shadow), predicted to be extracellular and GPI-anchored. (nih.gov)
GILZ protein and gene expression was quantified in blood neutrophils, along with markers of inflammation (CRP, extracellular DNA) or its resolution (Annexin A1). (springeropen.com)

The history of protein dynamics traces back to 1935, when Schoenheimer and Rittenberg synthesized the first isotopologs of biological molecules to demonstrate the continuous renewal of proteins throughout life 14 , 15 . (nature.com)
In contrast to steady-state protein abundance, which may be quantified directly in mass spectrometry (MS) by spectral intensity 16 , 17 or sampling frequency 18 , 19 , protein turnover rates cannot be predicted from steady-state data 20 , requiring instead methods that can distinguish old and new protein molecules in mass spectra 11 , 21 . (nature.com)

Gentle sample isolation is essential to prevent bias against vulnerable cell types while fast isolation is desirable to reduce degradation of nucleic acids. (lu.se)

18 With the exception of the ovarian neoplasms, the same carcinomas analyzed for gene expression were also examined for HE4 by immunohistochemistry on tissue microarrays (see below). (nature.com)
To define the incidence and characteristics of Lynch syndrome-related small-intestinal adenocarcinomas, meticulous familial and clinical histories were obtained from 195 patients with small-intestinal adenocarcinoma, and MMR protein immunohistochemistry, microsatellite instability, MLH1 methylation, and germline mutational analyses were performed. (oncotarget.com)

Welding fume exposure had rapid effects on the circulating leukocyte population as identified by relative mRNA expression changes. (cdc.gov)
The expression levels of caspase‑3, Bax and Bcl‑2 mRNA were determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). (spandidos-publications.com)

CALIXAR's native membrane protein expression service increases the success of producing reliable targets or antigens by adapting the expression conditions for each specific target. (calixar.com)
Protein analysis -Specific monoclonal antibodies ( CD9 , CD63 , and CD81 ) allow for detection of cellular and exosomal antigens by Western blot analysis . (thermofisher.com)

Using an integrated platform of metabolic labeling, high-resolution mass spectrometry and computational analysis, we report here a comprehensive dataset of the in vivo half-life of 3,228 and the expression of 8,064 cardiac proteins, quantified under healthy and hypertrophic conditions across six mouse genetic strains commonly employed in biomedical research. (nature.com)

If a membrane protein expresses at a high level, almost certainly some of this protein will be found in the insoluble fraction. (neb.com)
CALIXAR provides both purified membrane proteins that are off the shelf ready for use as well as membrane protein extraction kits to clients to work with the highest quality membrane proteins on the market. (calixar.com)
CALIXAR's membrane protein isolation service extracts native, wild-type, unmutated and untruncated targets so that pharmaceutical companies can produce antibodies and/or discovering primary leads. (calixar.com)
CALIXAR's ligand binding assays services provide key information on ligand interactions with any membrane protein targets. (calixar.com)
CALIXAR has been successfully supporting membrane protein research from our customers for over a decade now. (calixar.com)
We also offer membrane protein services to support customers in early research phases (up to Pre-Clinical), or for companies who may be looking for a contract research organization (CRO), or Membrane Protein service providers to supplement their own Membrane Protein capabilities. (calixar.com)

Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A-coated magnetic beads. (nih.gov)
Here, we show that we were able to use small amounts of antibodies (50 ng per target) in this manner for efficient multiplex analysis of quantitative levels of proteins in a human HeLa cell lysate. (nih.gov)
A lately developed and validated immunotagging technique makes use of three transcription issue antibodies to concurrently isolate enriched neuronal (NeuN+), astrocyte (paired field protein 6 (PAX6)+NeuN-), and oligodendrocyte progenitor ( OLIG2 +NeuN-) nuclei populations from non-diseased, recent (unfixed) snap-frozen postmortem human temporal neocortex tissue. (aetox.com)

Through a proteomic analysis, the researchers found that regional bacterial populations show protein expression differences. (genomeweb.com)
Based on their finding of strong signals of genetic compartmentalization, regional isolates with similar protein expression profiles, and spatially distinct distribution of bacterial phenotypes, the researchers said that their study indicates that isolation in different lung regions helped drive the diversification of P. aeruginosa in the lungs of cystic fibrosis patients. (genomeweb.com)

Here, we examined the expression of the HE4 gene and protein in a large series of normal and malignant adult tissues by oligonucleotide microarray and tissue microarray, respectively. (nature.com)
Whole blood was used for cellular differential counts, RNA isolation with subsequent microarray and Ingenuity Pathway Analysis, and secondary stimulation with LPS utilizing TruCulture technology. (cdc.gov)

Membrane proteins are one of the most challenging drug targets to produce and purify, because of their strong bond with the lipid bilayer, involving electrostaticity, hydrogen, and other types of binding. (calixar.com)
Used cell isolation techniques to purify PBMCs from blood (Ficoll), select monocytes and CD4 T cells, differentiate and harvest dendritic cells and macrophages. (livecareer.com)

We report evidence from cDNA isolation and expression analysis as well as analyses of genome, expressed sequence tag (EST), cDNA and expression databases for a new gene named SPRN (shadow of prion protein). (nih.gov)

Other lung cell types: Assistance with the isolation of lung fibroblasts and other cell types may be obtained with special planning through the core. (wustl.edu)
Studies have shown that periodontitis is associated with altered expression of inflammatory mediators in gingival fibroblasts and periodontal membrane cells ( 3 , 4 ). (spandidos-publications.com)

The kit is compatible with all protocols for exosome isolation. (thermofisher.com)
Gene expression analysis protocols and training in methods are available. (wustl.edu)
Cell function and gene expression analysis: Protocols and assistance with routine collection for protein and RNA are available. (wustl.edu)
Protocols and training: Protocols for cell isolation, culture and differentiation are available. (wustl.edu)
In case no pre-existing expression constructs and/or protocols are available, we can design a strategy together with you. (embl.org)

Glucocorticoid-induced leucine zipper (GILZ) is a potent anti-inflammatory protein involved in neutrophil apoptosis and the resolution of inflammation. (springeropen.com)
The present study explored the effect of NAC on LPS‑induced apoptosis of HUVECs and determined the participation of the p38 mitogen‑activated protein kinase (MAPK) pathway in the process of apoptosis. (spandidos-publications.com)
A series of caspases proteins have been shown to be activated by the death induced signal complex [a form of death ligand that binds to the corresponding death receptor on the cell surface ( 13 )], which in turn induces apoptosis. (spandidos-publications.com)

Membrane proteins play key roles in life processes, which is why they are attractive targets for drug discovery projects . (calixar.com)
We hypothesized that analysis of GPCR expression would identify clear subsets of medulloblastoma and suggest distinct GPCRs that might serve as molecular targets for both imaging and therapy. (biomedcentral.com)

Structural changes were detected by transmission electron microscopy (TEM), and levels of the hepatic fibrosis-related proteins collagen I (Col-I), alpha-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured by western blotting and enzyme-linked immunosorbent assay (ELISA). (biomedcentral.com)
Analyzed protein concentration in cellular extracts with BSA assay, and protein expression by Western Blotting. (livecareer.com)

Plant cell culture was used to produce FDA-approved glycosylated lysosomal protein-drug, and additional drug candidates. (wikipedia.org)
The results of these analyses revealed that the differentially expressed exosomal miRNAs participate in multiple biological processes, such as gene expression, synthesis of biomolecules, cell development, differentiation, and signal transduction, among others. (hindawi.com)
Their gene expression during brain development and within cell types highlights how they relate to ASD. (springer.com)
This can result in loss of cell viability or loss of the expression plasmid. (neb.com)
However, limited data are available about sequence variability in CD8 + T cell epitope regions of mumps virus (MuV) proteins. (mdpi.com)
Now it is possible to isolate intact exosomes from cell culture media or any body fluid using the flexible and scalable Total Exosome Isolation reagents. (thermofisher.com)
Total exosomes enriched from cell culture (by using the Total Exosome Isolation reagents or ultracentrifugation) can be further purified into specific subpopulations by immunomagnetic capture. (thermofisher.com)
However, adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas had moderate or high levels of HE4 expression. (nature.com)
To analyse expression of transcription factors which control the Th1 cell commitment in CD. (bmj.com)
For protein expression we use either the Sf21 or the Hi5 insect cell lines. (embl.org)
They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)
Indeed, most live cell applications of PTS utilize single split inteins for the purpose of N/C-terminal tagging 16 - 18 or manipulating protein assembly/expression 19 , 20 . (biorxiv.org)
We have adopted an interdisciplinary approach to disease research, from cell biology to protein expression and chemical synthesis. (edu.au)
18 ] who found that preventive or therapeutic administration of the cell-permeable TAT-GILZ fusion protein improved resolution in a mouse model of LPS-induced pleurisy. (springeropen.com)
Thus, prior to initiating a project at the Single-Cell Genomics Platform, users are asked to thoroughly evaluate a cell or nucleus isolation strategy suitable for their target tissue and research question. (lu.se)
Retinoschisin is a secreted protein found throughout the retina and is involved in intercellular adhesion, retinal cellular organization, cell-cell interactions within the inner nuclear layer, as well as synaptic connection between photoreceptors and bipolar cells. (medscape.com)
This protein controls zinc uptake across the plasma membrane of various cell types, including the intestine. (medscape.com)

These include both commercially available proteins (for example most of the enzymes used in the molecular biology laboratory), and those that are generated in the course specific research projects. (wikipedia.org)

While expression patterns of many proteins in human medulloblastoma subgroups have been discerned, the expression pattern of GPCRs in medulloblastoma has not been investigated. (biomedcentral.com)

For baculovirus-mediated expression in insect cells, we require expression vectors compatible with the Bac-to-Bac system (for example pFastBac, MultiBac, biGBac or MacroBac vectors). (embl.org)

Regulation of proteome integrity requires chaperone-assisted folding of unfolded proteins, dissolution of misfolded aggregates, proteolytic removal of proteins, and other concerted proteostatic processes 1 , 2 . (nature.com)
Production of the GFP-tagged P9 vesicles required P12, which protected the fusion protein against proteolytic cleavage. (biomedcentral.com)

The cystovirus Pseudomonas phage phi6 has an envelope that harbors five viral membrane proteins and phospholipids derived from the cytoplasmic membrane of its Gram-negative host. (biomedcentral.com)
Proteínas transcritas a partir de la región E1B del genoma de ADENOVIRUS, que estan involucradas en la regulación de los niveles precoces y tardios de expresión genética viral. (bvsalud.org)
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression. (bvsalud.org)

Elucidating tumorigenic pathways is a secondary benefit to identifying differential GPCR expression patterns in medulloblastoma tumors. (biomedcentral.com)
Proteins with quantified dynamics belong to over 10 major cellular compartments and over 200 known pathways. (nature.com)
Key proteins in mitochondria and metabolic pathways are encompassed, in addition to contractile machineries and sarcolemmal signaling proteins. (nature.com)

By transporting "cargos" such as proteins, RNAs, DNAs, and lipids [ 26 ], exosomes regulate the eventual fate of recipient cells. (hindawi.com)
NanoSight instrument -Exosomes recovered with Total Exosome Isolation reagent can be sized and counted using a NanoSight instrument. (thermofisher.com)

Knowledge of the expression patterns of HE4 in our survey is useful for application in histopathologic diagnosis, and should be taken into consideration in future studies that examine the role of HE4 as a serological tumor biomarker or as a target for gene-based therapy. (nature.com)
HER2 expression switching in circulating tumor cells (CTC) in breast cancer is dynamic and may have prognostic and predictive clinical implications. (bvsalud.org)

This study confirms that alterations in the expression of exosomal miRNAs can promote osteogenic differentiation of ADSCs, which also provides the foundation for further research on the regulatory functions of exosomal miRNAs in the context of ADSC osteogenesis. (hindawi.com)

We report here a large dataset of protein turnover dynamics in the heart of six common genetic strains of mice, acquired under both normal and hypertrophic conditions. (nature.com)
Co-administration of lipopolysaccharide in our culture media causes an inflammatory response in the enteroids leading to histologic, genetic, and protein expression alterations similar to those seen in human necrotizing enterocolitis. (jove.com)

The HE4 (WFDC2) gene encodes a WAP-type four disulphide core domain-containing protein with a presumptive role in natural immunity. (nature.com)

The effect of gliadin and IFN-γ on expression of T-bet was examined in an ex vivo culture of biopsies taken from normal and treated CD patients. (bmj.com)
The dataset contains over 1.92 million data points in protein isotope labeling kinetics, culminating as the in vivo turnover rates of 3,228 cardiac proteins and the expression levels of 8,064 proteins. (nature.com)
GILZ was expressed in about ¾ of the corticosteroid-treated patients and its expression could also occur independently of corticosteroids, suggesting that inflammatory signals may also induce neutrophil GILZ expression in vivo. (springeropen.com)

The abnormal wound healing is due to impaired limbal epithelial stem cells, and the new research, published online July 18 in Diabetologia , involved isolation of those cells from 30 donor eyes of humans with and 23 without diabetes. (medscape.com)

Proteomics is the large-scale analysis of protein expression and post-translational modifications. (cns.org)

Envelope protein of the hepatitis B virus marketed as Engerix-B by SmithKline Beecham HPV Vaccine proteins Polyphenol oxidases (PPOs): These include both catechol oxidases and tyrosinases. (wikipedia.org)

A P9-GFP fusion protein was used to study the targeting of heterologous proteins into P9 vesicles. (biomedcentral.com)

However, isolation is still required for the studies of the whole ribosome. (wikipedia.org)
When designing expression constructs, make sure that the vector does indeed encode a properly spaced ribosome binding site and also analyze for secondary structure at the five prime end of the messenger RNA. (neb.com)
If you find that the five prime UTR is not optimized for expression then simply change the vector, the ribosome binding site, or adding an affinity tag at the N-terminus often enhances expression. (neb.com)

And finally, many proteins from higher organisms require complex disulfide bonds for stability and proper folding. (neb.com)
CALIXAR's Thermal Stability Assays provides information on expression levels, stability and ligand binding of membrane proteins. (calixar.com)
Protein stability is a major regulatory principle of protein function and cellular homeostasis. (nature.com)
Does the protein require co-factors for stability or functionality? (embl.org)
Does the protein require interaction partners for stability (→ co-expression)? (embl.org)

What is the cellular localization of the protein? (embl.org)

Core users can be instructed on all methods for culture, manipulation and evaluation of protein and gene expression. (wustl.edu)
Manipulation of proteins by chemical modification is a powerful way to decipher their function or harness that function for therapeutic purposes. (biorxiv.org)

Do you know whether there are disulfide bonds present in the native protein? (embl.org)

We investigated the role of Echinococcus multilocularis (Echinococcus genus) PSCs in hepatic fibrosis by examining structural changes and measuring hepatic fibrosis-related protein levels in cocultures of PSCs and human HSCs. (biomedcentral.com)
Database information indicates expression of SPRN in embryo, brain and retina of mouse and rat, hippocampus of human, and in embryo and retina of zebrafish, and we directly confirmed a strikingly specific expression of the mammalian (human, mouse, rat) transcripts in whole brain. (nih.gov)
In a series of 175 human adult tumors, gene expression was highest in ovarian serous carcinomas. (nature.com)
Human epididymis protein 4 (HE4) was first identified in the epithelium of the distal epididymis and originally predicted to be a protease inhibitor involved in sperm maturation. (nature.com)
The expression of miRNAs was evaluated (in pool) using TaqMan® Array Human MicroRNA Cards v2.0. (bvsalud.org)
More recently, the gene SLC39A4 was found to encode a solute carrier protein called human zinc/iron-regulated transporterlike protein (hZIP4). (medscape.com)

Assistance with techniques for in situ analysis of gene expression methods (e.g., immunofluorescence) can be provided. (wustl.edu)
A meticulous patient history taking, MMR protein immunolabeling, and germline MMR gene mutational analysis are important for the diagnosis of Lynch syndrome-related small-intestinal adenocarcinomas. (oncotarget.com)

Anatomy24

Organisms15

Diseases11

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment58

Psychiatry and Psychology1

Phenomena and Processes63

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science4

Health Care3

Purification9

Characterization6

Recombinant16

Genes11

Intercellular Signalin1

Escherichia2

Soluble2

Peptides4

Intracellular5

Vitro6

Mammalian cells1

Receptors1

Functional6

Phosphorylation2

Bacteria2

Coli5

Ribosomal3

Extracellular5

Molecules2

Nucleic acids1

Immunohistochemistry2

MRNA2

Antigens2

Mass Spectrometry1

Membrane protein6

Antibodies3

Bacterial2

Microarray2

Purify2

CDNA1

Fibroblasts2

Protocols5

Apoptosis3

Targets2

Assay2

Cell18

Enzymes1

GPCRs1

Insect cells1

Proteolytic2

Viral3

Pathways3

Exosomes2

Tumor2

Differentiation1

Genetic2

Disulphide1

Vivo3

Humans1

Proteomics1

Vaccine1

Fusion Protein1

Ribosome3

Stability5

Cellular1

Manipulation2

Disulfide bonds1

Human6

Analysis2