• In total, 145 endophytic bacterial strains were isolated and further grouped using either ERIC-PCR or BOX-PCR, resulting in 76 groups composed of different genera predominantly belonging to the Gammaproteobacteria. (biomedcentral.com)
  • The EXTRACTME PLASMID DNA 96-WELL KIT with filter plates is designed for high-throughput and efficient purification of high quality plasmid DNA from recombinant Escherichia coli strains. (blirt.eu)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
  • The project will begin by evaluating different primers and probes on known bacterial strains with the goal of creating a multiplex PCR where all three bacteria are included in the same real-time PCR. (lu.se)
  • The Genomic DNA Clean & Concentrator-25 (DCC) is for quick (5 minute) recovery of ultra-pure, large-sized DNA(e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from any enzymatic reaction or impure preparation (e.g. (thomassci.com)
  • Once the target DNA has been successfully ligated into the plasmid vector, the plasmid must be transferred into the host cell for replication and cloning. (igem.org)
  • Lab experiments introduce a variety of molecular biology techniques such as replica plating, bacterial conjugation and transformation, the isolation and restriction enzyme cleavage of plasmid DNA, and restriction mapping. (monroecc.edu)
  • In the first isolation step, the plasmid DNA is released from bacterial cells by alkaline lysis. (blirt.eu)
  • DNA are separated with the use of Plasmid Filter Plate. (blirt.eu)
  • The purified plasmid DNA is eluted using a low ionic strength buffer (Elution Buffer) or water (pH 7.0-9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, ligation and so forth, or stored until ready to use. (blirt.eu)
  • The quality of each production batch (LOT) of the EXTRACTME PLASMID DNA 96-WELL KIT is tested using standard QC procedures. (blirt.eu)
  • Recommendations for efficient plasmid DNA purification using MACHEREY-NAGEL NucleoBond Xtra Kits. (mn-net.com)
  • No matter the sample origin, you need high yields of microbial DNA and unbiased results for analysis of microbial communities. (qiagen.com)
  • Our microbial DNA extraction kits provide you with high yields of high-quality DNA and enable efficient lysis, thereby preventing bias towards abundant species. (qiagen.com)
  • XpressDNA Soil Kit provides a magnetic nanoparticle based DNA extraction method for high quality microbial genomic DNA purification from soil samples without a bead beating step. (thomassci.com)
  • For instance, in June 2022 Molzym received a fourth round of funding to strengthen its offering in microbial DNA isolation technology. (grandviewresearch.com)
  • Traditional microbial tests focus on the isolation of specific bacterial organisms in a laboratory, which is time consuming and reliant on specialised infrastructure and staff. (embl.org)
  • In our study, we report a simple, cost-effective workflow to assess and monitor microbial freshwater ecosystems with targeted nanopore DNA sequencing," says Maximillian Stammnitz, formerly a PhD student in Cambridge, now a postdoc at the Centre for Genomic Regulation (CRG) in Barcelona. (embl.org)
  • It induces both local effects in the gut, as well as alterations in distant organs through stimuli generated by bacteria, structural bacterial components, and microbial metabolites [ 7 ]. (biomedcentral.com)
  • Amplified DNA genes were detected by gel electrophoresis starting from small volume and cells concentration down to 10^2 CFU/ml. (uwaterloo.ca)
  • Agarose gel electrophoresis was carried out on the qPCR products using 1.5% agarose gel with a standard DNA ladder (100 bp), visualized under UV transilluminator and the image taken using digital camera. (scialert.net)
  • My areas of expertise include plant science, light microscopy, protein and agarose gel electrophoresis, DNA extraction and PCR, Western blotting, aseptic technique and plant, bacteria and human cell culture. (ncl.ac.uk)
  • The purified DNA concentration, quality and stability are evaluated by gel electrophoresis and spectrophotometer. (blirt.eu)
  • Also, the cell lysate containing the DNA released from bacterial cells after lysing with the developed antibacterial PPM columns is ready to be used by PCR with no further purification needed. (uwaterloo.ca)
  • In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)
  • bacterial genome editing using of 'suicide' vectors for allelic exchange. (uibk.ac.at)
  • A high-quality reference sequence for the Brassica napus genome is not yet available, thus studies of specific regions of the Brassica napus genome at sequence level have relied on the analysis of bacterial artificial chromosomes (BACs) or BAC contigs. (biomedcentral.com)
  • The demand for kits used in the isolation and purification of nucleic acids is on the rise, driven by the increasing number of research studies focused on comprehending genome-based diseases such as Huntington's disease, polyketonuria, and others. (grandviewresearch.com)
  • Therefore, in this study, to select P. odoriferum -specific genes, species-specific genes were selected using the data of the P. odoriferum JK2.1 whole genome and similar bacterial species registered with NCBI. (ppjonline.org)
  • To create this benchmark for a broadly consented son in a Personal Genome Project trio with broadly available cells and DNA, the Genome in a Bottle Consortium integrated 19 sequence-resolved variant calling methods from diverse technologies. (nabsys.com)
  • We demonstrate that whole genome maps, constructed from electronic detection of tagged DNA, hundreds of kilobases in length, can be used effectively to facilitate calling of structural variants ranging in size from 300 base pairs to hundreds of kilobase pairs. (nabsys.com)
  • Isolation and Whole-Genome Sequencing of Environmental Campylobacter. (cdc.gov)
  • repetitive sequence elements in the members had been in contact with bacterial genome have shown con- patients for several hours. (who.int)
  • We also wanted to see how viable it was to use portable DNA sequencers to monitor bacteria species in rivers. (embl.org)
  • By contrast, the method used in this study - called environmental metagenomics - can identify the DNA of the many bacteria present in a sample of river water. (embl.org)
  • The objective of this study was to determine if environmental sampling methods can skew the type of bacteria that is recovered, resulting in loss of traceability of the bacterial lineage. (usda.gov)
  • 2013. Isolation of oxalotrophic bacteria able to disperse on fungal mycelium. (unine.ch)
  • 2013. Identification of active oxalotrophic bacteria by bromodeoxyuridine DNA-labeling in a microcosm soil experiment. (unine.ch)
  • has been identified as the 2nd most consensus (ERIC) sequences which · 60 environmental samples were tak- frequent organism causing ventilator- are common to Gram-negative enteric en throughout the ICU, including associated pneumonia, the 4th most bacteria [11,12]. (who.int)
  • The Quick-gDNA Microprep is for the convenient, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. (thomassci.com)
  • The proven magnetic bead-based NucleoMag VET kit gets its little brother: MACHEREY-NAGEL is releasing a new silica-membrane based kit for the isolation of viral RNA/DNA and bacterial DNA from diverse veterinary sample specimens. (mn-net.com)
  • Tri-Xtract™ is suitable for the isolation of total RNA, including mRNA, hnRNA, ribosomal RNA and tRNA, from small quantities of tissues (50-100mg) and cells (5 x 10 6 ) from human, animal, plant, yeast, bacterial and viral origin. (gbiosciences.com)
  • Norgen's Total Genomic DNA Purification Kits allow for the isolation of total genomic DNA of all sizes from a wide range of sample inputs including cells, tissues, urine, blood, stool, FFPE samples, plants and soil. (norgenbiotek.com)
  • Digestion buffer for use with Zymo Research purification kits for DNA and RNA extractions. (thomassci.com)
  • The market comprises of a wide range of DNA and RNA isolation/extraction and purification kits for preparing samples and libraries. (grandviewresearch.com)
  • In this study, with different bacterial cocktail mixes, analyzed as pure or spiked in a synthetic fecal matrix, we evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for on-site application. (altmetric.com)
  • The application of porous polymeric monolith (PPM) column as an e effective tool for bacterial cell lysis is demonstrated in this thesis. (uwaterloo.ca)
  • The bacterial cell wall is mechanically sheared (mechanical-shear lysis) by flowing through the narrow porous medium of the PPM column, but it is also damaged and disintegrated by physical contact (contact-killing lysis) with the antibacterial polymeric biocide covering the porous surface. (uwaterloo.ca)
  • The developed antibacterial PPM columns can be potentially used in developing commercial macro-columns for cell lysis like the columns commercially available for DNA isolation. (uwaterloo.ca)
  • The developed cell lysis technique eff efficiently lysed numerous gram-negative and gram-positive bacterial species with no chemical or enzymatic reagents utilized, power consumption required, complicated design and fabrication processes. (uwaterloo.ca)
  • Furthermore, the lysis efficiency of the PPM was improved by tuning their hydrophobic-hydrophilic balance and determining the optimal flow rate, at which the bacterial cell walls were sufficiently mechanically sheared through the porous medium of the column to disrupt the cell membrane by physical contact with the antibacterial polymeric biocide covering the pore surface. (uwaterloo.ca)
  • Also the e effect of the cross-linking monomer on bacterial growth inhibition, lysis efficiency and the mechanical stability of the PPM column within the microfluidic channel, using three different cross-linking monomers was studied. (uwaterloo.ca)
  • The cell lysis e efficiency of the biochips were characterized by qualitatively (semi-quantitatively) detecting DNA and quantitatively determining DNA concentration in the crude lysate collected at the outlet of the biochip. (uwaterloo.ca)
  • To determine the incidence and etiology of meningitis in Turkey, cerebrospinal fluid (CSF) samples were obtained prospectively from children (1 month-17 years of age) with a clinical diagnosis of acute bacterial meningitis. (cdc.gov)
  • Tri-Xtract™ is a convenient, ready-to-use reagent designed for the isolation of total RNA that is free from protein and DNA contamination. (gbiosciences.com)
  • Total RNA is isolated in under an hour and the subsequent isolation of DNA and protein in less than three hours. (gbiosciences.com)
  • The Bacterial Genomic Dna Isolation Kit Rkt09 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (abeurope.info)
  • Genomic DNA was extracted from air samples using a PowerSoil® DNA Isolation Kit. (cdc.gov)
  • For the rapid and simple purification of bacterial and host DNA from stool and fecal samples. (norgenbiotek.com)
  • To take the best advantage of long-read sequencing, DNA extraction methods must be able to isolate pure high molecular weight (HMW) DNA from complex metagenomics samples, without introducing any bias. (altmetric.com)
  • About 200 clinical samples were obtained for S. aureus isolation, identified and characterized by using standard microbiological procedures. (scialert.net)
  • Two hundred clinical wound and burn samples were obtained with sterile cotton swabs from tertiary health care facilities within Delta state, Nigeria, for S. aureus isolation. (scialert.net)
  • For instance, Thermo Fisher Scientific offers PrepSEQ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit designed for the extraction of mycoplasma genomic DNA from samples. (grandviewresearch.com)
  • They used a smartphone-sized DNA sequencing device to monitor water samples taken from nine locations on the river Cam. (embl.org)
  • River water was collected for isolation of Campylobacter by three methods (filtering of free-catch water, sediment grab samples, and placement of large cotton swabs in the water) and the recovered organisms were compared by a molecular genetic typing method based on DNA sequence. (usda.gov)
  • A total of 457 kidney samples were examined for both bacterial agents using nested and conventional PCR as well as bacterial cultivation on KDM-2, histological examination and immunohistochemistry. (biomedcentral.com)
  • How to Process Sputum Samples and Extract Bacterial DNA for Microbiota Analysis. (cdc.gov)
  • Amongst the 6 tested methods, the Quick-DNA HMW MagBead Kit (Zymo Research) was considered as the most suitable for Nanopore sequencing and would be recommended for bacterial metagenomics studies using this technology. (altmetric.com)
  • In recent years, devices for mobile DNA analysis have made environmental metagenomics more accessible, allowing real-time sequencing. (embl.org)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
  • Furthermore, inhibitors and background DNA and RNA can lead to inaccurate quantification of nucleic acids and inhibit your downstream applications. (qiagen.com)
  • The global nucleic acid isolation and purification market size was valued at USD 5.11 billion in 2022 and is expected to grow at a compound annual growth rate (CAGR) of 10.06% from 2023 to 2030. (grandviewresearch.com)
  • Privatization of the production of therapeutics with commercial potential, such as vaccines and peptide-based drugs, to suffice healthcare needs is driving the demand for large-scale separation instruments Thus, the nucleic acid isolation and purification industry exhibits lucrative growth potential. (grandviewresearch.com)
  • Rapidly evolving biotechnological applications in various industries, such as food processing, agriculture, recycling & waste management, and energy & environmental, are projected to drive the adoption of nucleic acid isolation and purification. (grandviewresearch.com)
  • To address these challenges, companies are introducing novel isolation kits to facilitate the recovery of nucleic acids. (grandviewresearch.com)
  • The DNA purification procedure utilizes spin 96-minicolumns plates with membranes which efficiently and selectively bind nucleic acids. (blirt.eu)
  • The nucleic acid purification kit manufacturer MACHEREY-NAGEL and Chroma ATE have collaborated to develop verified methods for the automated DNA/RNA purification from various sample materials on the MagXtract 3200. (mn-net.com)
  • N. meningitidis is now considered to be the leading cause of bacterial meningitis in many regions of the world, causing an estimated 1.2 million cases of bacterial meningitis and sepsis worldwide each year ( 10 , 11 ). (cdc.gov)
  • The identification of species was addressed through a combination of biochemical and genetic methods (API ® 50 CHL test, 16S rDNA and recA gene sequences, Amplified Ribosomal DNA Restriction Analysis -ARDRA- and 16S-26S intergenic region analysis). (mdpi.com)
  • Furthermore, this kit allowed an accurate detection, by Nanopore sequencing, of almost all the bacterial species present in a complex mock community. (altmetric.com)
  • Researchers from EMBL's European Bioinformatics Institute (EMBL-EBI) and the University of Cambridge have shown that portable DNA sequencing can easily monitor bacterial species in a river ecosystem. (embl.org)
  • R. salmoninarum is the causative agent of bacterial kidney disease, a Gram-positive bacterium mostly known for causing chronic infections in salmonid fish, while multiple species belonging to the Mycobacterium genus have been associated with mycobacteriosis in fish as well as in human. (biomedcentral.com)
  • It was confirmed that the selected primer set formed a specific amplicon of 360 bp only in the DNA of P. odoriferum using 29 Pectobacterium species and related species. (ppjonline.org)
  • We also provide training for students, researchers and collaborators in the techniques of medium (agar/broth) preparation for bacterial cultures, antibiotic susceptibility testing (minimum inhibitory concentration determination), molecular cloning and population analysis for hetero-VISA detection. (ukm.my)
  • Hitachi Exocomplete Laboratories manufactures the bacterial genomic dna isolation kit rkt09 reagents distributed by Genprice. (abeurope.info)
  • Little is known about the genomic diversity of Escherichia coli in healthy children from sub-Saharan Africa, even though this is pertinent to understanding bacterial evolution and ecology and their role in infection. (peerj.com)
  • Ablation of DNMT3A in Caco-2 cells results in global DNA hypomethylation, which is linked to impaired regenerative capacity, transepithelial resistance and intercellular junction formation. (nature.com)
  • Whereas DNMT1 is the key maintenance methyltransferase, DNMT3A and DNMT3B represent the main de novo methyltransferases, as they can bind unmethylated DNA and establish novel DNA methylation marks not only during early development but also in differentiated cells in a signal-dependent manner 9 . (nature.com)
  • Much of the work that has been done to understand how cells work has involved studying parts of a cell in isolation. (elifesciences.org)
  • In order to do this, the bacterial cells must first be made "competent. (igem.org)
  • Several methods to make bacterial cells competent exist, such as the calcium chloride method and electroporation. (igem.org)
  • 7. Pipette contents of tube up and down a few times then take 2 μl of the DNA solution and add to the competent cells. (igem.org)
  • The antibacterial monolithic columns possess the aptitude to constrain the growth, kill and e efficiently lyse gram-negative and gram-positive bacterial cells. (uwaterloo.ca)
  • Sputum was assessed for inflammatory cells, and DNA was extracted from sputum cell suspensions and supernatants for C. pneumoniae detection using real-time PCR. (ersjournals.com)
  • 2013. Bacterial farming by the fungus Morchella crassipes. (unine.ch)
  • In this study, we evaluated antimicrobial residues, bacterial diversity and ARGs in two important watersheds, Guandu and São João, that supply drinking water to Rio de Janeiro city, Brazil. (researchsquare.com)
  • DNA probes and mycolic acid high-pressure liquid chromatography performed on a culture from a clinical specimen) are acceptable under this criterion. (cdc.gov)
  • A series of comparative critical threshold (2-deltadeltaCT) real-time PCR was used to quantify targeted bacterial (16S rRNA) genes and major ARGs. (cdc.gov)
  • A chronic bacterial infection caused by Mycobacterium tuberculosis , usually characterized pathologically by the formation of granulomas. (cdc.gov)
  • 2013. Gains of bacterial flagellar motility in a fungal world. (unine.ch)
  • By using fluorometry, the ethidium bromide (EtBr) intercalation assay was utilized as an indicator of the presence of DNA in the cell lysate and the DNA concentration was determined by UV-Vis spectrophotometry. (uwaterloo.ca)
  • Then the lysate is applied to the purification minicolumn membrane and the DNA is bound. (blirt.eu)
  • Des isolats suspects de Staphylococcus ont d'abord été identifiés par l'analyse biochimique classique, puis les souches confirmées par amplification en chaîne par polymérase (29 isolats de S. aureus et 17 isolats de S. saprophyticus) ont été analysées pour déterminer leur sensibilité aux différents antimicrobiens. (who.int)
  • For instance, in September 2022, Akadeum Life Sciences, Inc. announced the early access launch of a large-scale cell isolation kit for leukopaks. (grandviewresearch.com)
  • Secondary bacterial infection of the skin, lungs, and bones may develop. (msdmanuals.com)
  • Determination of the etiology of bacterial meningitis and estimating cost of disease are important in guiding vaccination policies. (cdc.gov)
  • Despite many new antibacterial agents, bacterial meningitis fatality rates remain high, with reported rates between 2% and 30% ( 3 , 4 ). (cdc.gov)
  • Genetic variants in the DNA methyltransferase 3 A (DNMT3A) locus have been associated with inflammatory bowel disease (IBD). (nature.com)
  • In addition to the comparison of the quality, quantity and purity of the extracted DNA, the performance obtained when doing Nanopore sequencing on a MinION flow cell was also tested. (altmetric.com)
  • The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity. (blirt.eu)
  • He identified periodic acid-Schiff (PAS)-staining granules, most likely representing degenerating bacterial forms, within macrophages isolated from the small bowel as well as other tissue and fluid specimens (eg, pericardium, endocardium, lymph nodes, synovia, lung, brain, meninges) obtained from patients in whom Whipple disease was suspected. (medscape.com)
  • Patented Inhibitor Removal Technology removes inhibitory components that can negatively affect analysis and efficient host DNA depletion, enabling efficient use of NGS capacities. (qiagen.com)
  • From the obtained results, the Quick-DNA HMW MagBead Kit (Zymo Research) was selected as producing the best yield of pure HMW DNA. (altmetric.com)
  • Efficient screening of bacterial artificial chromosome (BAC) libraries with polymerase chain reaction (PCR)-based markers is feasible provided that a multidimensional pooling strategy is implemented. (biomedcentral.com)
  • Illumina Sequencing Platforms - different protocols of DNA, cDNA, amplicon libraries preparation and sequencing. (uibk.ac.at)
  • Multiplex PCR was used to detect DNA evidence of Streptococcus pneumoniae , Haemophilus influenzae type b (Hib), and Neisseria meningitidis . (cdc.gov)
  • The laboratory has equipments such as a Biosafety Level 2 (BSL 2) cabinet, incubator shaker and electroporator to support upstream experiments such as bacterial isolation and DNA extraction. (ukm.my)
  • The Gram-negative bacterium Pseu- siderable potential as DNA typing staff member 30 mL of sterile nutri- domonas aeruginosa is frequently associ- tools in the laboratory [10]. (who.int)