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Neisseria meningitidisNeisseriaNeisseria gonorrhoeaeMeningococcal InfectionsNeisseria meningitidis, Serogroup BMeningitis, MeningococcalNeisseria meningitidis, Serogroup W-135Neisseria meningitidis, Serogroup CNeisseria meningitidis, Serogroup ANeisseria meningitidis, Serogroup YNeisseria lactamicaMeningococcal VaccinesSerotypingBacterial Outer Membrane ProteinsBacterial ProteinsGonorrheaBacterial CapsulesDNA, BacterialPorinsMolecular Sequence DataAntibodies, BacterialBlood Bactericidal ActivityTransferrin-Binding ProteinsAntigens, BacterialCarrier StatePolysaccharides, BacterialTransferrin-Binding Protein BNeisseriaceae InfectionsMicrobial Sensitivity TestsGenes, BacterialBacterial Typing TechniquesSequence Analysis, DNAMeningitis, BacterialAnti-Bacterial AgentsSulfadiazineSerum Bactericidal Antibody AssayLipopolysaccharidesNeisseriaceaeBacterial VaccinesNeisseria cinereaIron-Binding ProteinsBase SequenceCerebrospinal FluidFimbriae, BacterialNasopharynxFimbriae ProteinsPolymerase Chain ReactionAmino Acid SequenceBacterial AdhesionAntigenic VariationPharynxDisease OutbreaksPhylogenyHaemophilus influenzaePenicillin ResistanceCulture MediaElectrophoresis, Gel, Pulsed-FieldDrug Resistance, BacterialPenicillin GGenetic VariationVirulenceSpecies SpecificityMeningitisMeningitis, HaemophilusPolyanetholesulfonateDrug Resistance, MicrobialBacteriological TechniquesGene Expression Regulation, BacterialVaccines, ConjugateMolecular EpidemiologyEscherichia coliBacteremiaMeningitis, PneumococcalPili, SexDNA FingerprintingAgglutination TestsMoraxellaTransformation, BacterialCross ReactionsN-Acetylneuraminic AcidGenome, BacterialPenicillinsGenotypeMultilocus Sequence TypingMutationStreptococcus pneumoniaeIronSialyltransferasesSialic AcidsLipid AUDPglucose 4-EpimeraseTransferrin-Binding Protein ABloodPhenotypeCytidine Monophosphate N-Acetylneuraminic AcidCeftriaxoneCluster AnalysisPolymorphism, Restriction Fragment LengthEpitopesComplement C6
HaemophilusClonal complexStreptococcusGenomeStrainCaused by the bacteriumGonorrhoeaeHumansCharacterizationStrains isolatedSerogroup Y IsolatesPathogensMeningitisCarriagePenicillinCeftriaxoneDiplococcusLactamicaSerogroupsRecombinationSusceptibilityAmpicillinDisease isolatesAntigensGenomicSequenceSusceptibleEpidemicOuter membraneSpeciesMeningococcusMeningococciNasopharynxMolecularPubMLSTCarbohydrate utilizationDiplococciSpecimensEpidemiologicIntracellularPharyngealVaccine2016
Haemophilus4
Clonal complex5
  • Isolates were sent to the National Microbiology Laboratory for confirmation of serogroup and further studies including phenotype and clonal complex identification. (canada.ca)
  • Prior to 2015, most invasive serogroup W isolates were identified as clonal complex 22 (ST-22 CC) and the increase in serogroup W in Canada in recent years has been associated with the replacement of the endemic ST-22 CC with the hyper-virulent ST-11 CC. (canada.ca)
  • Isolates of serogroup A had the same antigenic formula (A:4:P1.9), the same variable regions VR1, VR2 and VR3, and belonged to the same clonal complex (CC5). (who.int)
  • Of the 14 cases among persons with HIV in 2022 that were not related to the outbreak primarily among MSM, nine were caused by a single strain of N. meningitidis serogroup Y clonal complex CC174 sequence type ST-1466. (medscape.com)
  • Neisseria meningitidis ( N.meningitidis ) bacteria belonging to clonal complex 4821 (CC4821) have been mainly reported in China and have been characterized by a high resistance rate to ciprofloxacin (CIP). (biomedcentral.com)
Streptococcus2
Genome14
  • Draft Genome Sequence of a Neisseria meningitidis Serogroup C Isolate of Sequence Type 11 Linked to an Outbreak among Men Who Have Sex with Men. (pasteur.fr)
  • Here, we report the genome sequence of N. meningitidis strain LNP27256, an isolate of sequence type 11 linked to a recent outbreak among men who have sex with men in Europe. (pasteur.fr)
  • Genome-Based Characterization of Emergent Invasive Neisseria meningitidis Serogroup Y Isolates in Sweden from 1995 to 2012. (ox.ac.uk)
  • Whole-genome sequencing was performed on invasive serogroup Y isolates from 1995 to 2012 in Sweden (n = 186). (ox.ac.uk)
  • N. meningitidis isolates were evaluated for antibiotic susceptibility and characterised by whole genome sequencing. (bmj.com)
  • Genetic relationships among the meningococcal carriage isolates were determined using core genome multilocus sequence typing analysis. (bmj.com)
  • To assess within-host genetic changes in meningococci, we further used ultra-deep whole-genome sequencing of throat-blood strain pairs isolated from four patients suffering from invasive meningococcal disease. (uni-wuerzburg.de)
  • Isolates are characterized using whole genome sequencing to determine serogroup and molecular typing information. (medscape.com)
  • High-Quality Genome Sequence of an Escherichia coli O157 Strain Carrying an mcr-1 Resistance Gene Isolated from a Patient in the United States. (cdc.gov)
  • Complete Genome Sequences of Two Bordetella hinzii Strains Isolated from Humans. (cdc.gov)
  • Isolates were genome sequenced and then were assembled de novo and deposited into the Bacterial Isolate Genome Sequencing Database (BIGSdb). (ox.ac.uk)
  • Whole-genome MLST effectively distinguished between highly similar outbreak strain isolates, including those isolated from person-to-person transmission, and also highlighted how a few HGT events can distort the true phylogenetic relationship between highly similar clonal isolates. (ox.ac.uk)
  • Methods: Whole genome sequence data from Neisseria species, including Nme genomes from a diverse range of clonal complexes and capsule genogroups, and non- Neisseria species, were obtained from PubMLST and GenBank. (ox.ac.uk)
  • Genetic characterization of two G8P[8] rotavirus strains isolated in Guangzhou, China, in 2020/21: evidence of genome reassortment. (cdc.gov)
Strain5
  • Intercontinental spread of an epidemic group A Neisseria meningitidis strain. (cdc.gov)
  • For this surveillance, diagnostic laboratories throughout New Zealand refer specimens or bacterial isolates from their patients to ESR's national reference laboratories for strain identification and epidemiological typing. (cri.nz)
  • Serogroup B strain P1.19,15: F4- 28: ST-34 (cc32) accounted for 64.1% of IMD isolates. (nvspl.lt)
  • Using Neisseria meningitidis genomic diversity to inform outbreak strain identification. (cdc.gov)
  • MC 278, Neisseria meningitidis strain 278 (GenBank accession no. (bmj.com)
Caused by the bacterium2
Gonorrhoeae17
Humans2
  • It colonizes and infects only humans, and has never been isolated from other animals. (wikipedia.org)
  • Humans are the only hosts of N. meningitidis and can carry them harmlessly in the pharynx. (bmj.com)
Characterization2
  • however, the characterization of meningococcal isolates by molecular typing methods has not yet been performed. (nvspl.lt)
  • This facilitated an expanded MLST approach utilizing large numbers of loci for isolate characterization and discrimination. (ox.ac.uk)
Strains isolated2
  • Fingerprints generated with the endonucleases SfiI, SpeI and NheI each gave distinctive patterns for the clonal subgroups I-IV of serogroup A. Further, the endonucleases SpeI and, particularly, NheI were capable of resolving differences between various subgroup III strains isolated at different times and geographical locations. (ox.ac.uk)
  • Strains isolated during the 'new wave' pandemic, which was associated with the Haj, from Europe, America, and Africa, had a characteristic fingerprint pattern and appeared to be distinct from 'old wave' pandemic strains. (ox.ac.uk)
Serogroup Y Isolates1
  • These isolates were compared to a collection of serogroup Y isolates from England, Wales, and Northern Ireland from 2010 to 2012 (n = 143), which had relatively low serogroup Y incidence, and two isolates obtained in 1999 in the United States, where serogroup Y remains one of the major causes of IMD. (ox.ac.uk)
Pathogens4
  • An extensive collection of isolates for some bacterial pathogens are available through the Active Bacterial Core surveillance (ABCs) Isolate Bank. (cdc.gov)
  • Patient specimens and bacterial isolates from the communicable diseases and pathogens listed below should be referred routinely by diagnostic laboratories to the relevant ESR laboratory for typing/surveillance purposes (this does not include specimens for diagnostic testing, nor for requests on a fee for test basis). (cri.nz)
  • Using N. meningitidis as model organism, we tested the hypothesis that virulence of commensal pathogens is a consequence of within host evolution and selection of invasive variants due to mutations at contingency genes, a mechanism Some members of the physiological human microbiome occasionally cause life-threatening disease even in immunocompetent individuals. (uni-wuerzburg.de)
  • Using N. meningitidis as model organism, we tested the hypothesis that virulence of commensal pathogens is a consequence of within host evolution and selection of invasive variants due to mutations at contingency genes, a mechanism called phase variation. (uni-wuerzburg.de)
Meningitis12
  • Neisseria meningitidis is a leading cause of bacterial meningitis and sepsis among older children and young adults in the United States. (bizzieme.com)
  • How do we prevent Neisseria meningitidis from causing meningitis? (bizzieme.com)
  • Should patients with viral meningitis be isolated? (bizzieme.com)
  • A 9-month-old baby in septic shock with purpuric Neisseria meningitis skin lesions. (medscape.com)
  • Neisseria meningitidis, often referred to as the meningococcus, is a Gram-negative bacterium that can cause meningitis and other forms of meningococcal disease such as meningococcemia, a life-threatening sepsis. (wikipedia.org)
  • citation needed] Septicaemia caused by Neisseria meningitidis has received much less public attention than meningococcal meningitis even though septicaemia has been linked to infant deaths. (wikipedia.org)
  • Neisseria meningitidis (Gram-negative diplococcus) is responsible globally for invasive meningococcal disease (IMD), in particular septicaemia and meningitis. (bmj.com)
  • From April through October 1989, approximately 4000 cases of meningitis due to Neisseria meningitidis serogroup A were reported in Nairobi, an attack rate of 267 per 100,000 during this period. (cdc.gov)
  • A prime example of such a commensal pathogen is Neisseria meningitidis, which normally resides in the human nasopharynx but is also a leading cause of sepsis and epidemic meningitis. (uni-wuerzburg.de)
  • ABSTRACT Neisseria meningitidis, a leading cause of bacterial meningitis and other serious infections, is responsible for approximately one-third of cases of bacterial meningitis in the Children's Hospital of Tunis. (who.int)
  • Meningococcal meningitis is the most of meningococcal meningitis ( 12 ) and lance of meningococcal disease accord- common entity and the outcome is Europe, where isolates of serogroup B ing to the guidelines of the Tunisian usually good if patients are rapidly and are predominant and responsible for Department of Health. (who.int)
  • Here, a variety of quantitative techniques were employed to investigate multilocus sequence information from isolate collections of Neisseria meningitidis, a major cause of meningitis and septicemia world wide. (ox.ac.uk)
Carriage5
  • The prevalence of N. meningitidis carriage in men who have sex with men was high with a high rate of penicillin non-susceptible isolates. (bvsalud.org)
  • The sAniA protein was expressed in 8 out of 28 (29%) screened meningococcal carriage isolates. (bmj.com)
  • A carriage study was undertaken (n = 112) to ascertain the prevalence of Neisseria spp. (ox.ac.uk)
  • Insights on Population Structure and Within-Host Genetic Changes among Meningococcal Carriage Isolates from U.S. Universities. (cdc.gov)
  • The genetic differentiation between disease and carriage isolate collections indicated that, whereas certain genotypes were overrepresented among the disease-isolate collections (the "hyperinvasive" lineages), disease-associated and carried meningococci exhibited remarkably little differentiation at the level of individual nucleotide polymorphisms. (ox.ac.uk)
Penicillin4
  • However, 11 meningococcal disease cases reported in the United States during 2019-2020 had isolates that contained a bla ROB-1 β-lactamase gene associated with penicillin resistance, as well as mutations associated with ciprofloxacin resistance. (cdc.gov)
  • Laboratories should be aware that CDC recommends that healthcare providers use antimicrobial susceptibility testing (AST) to determine susceptibility of all meningococcal isolates to penicillin before changing from empirical treatment with cefotaxime or ceftriaxone to penicillin or ampicillin. (cdc.gov)
  • MICs of penicillin , ceftriaxone , rifampicin , azithromycin and ciprofloxacin were determined for 66 and 102 isolates from periods 1 and 2 respectively, according to CLSI. (bvsalud.org)
  • Four isolates were penicillin G-resistant and 73% of those penicillin G-susceptible showed a minimum inhibitory concentration from 0.064 μg/mL to 0.25 μg/mL. (bmj.com)
Ceftriaxone2
Diplococcus2
  • citation needed] N. meningitidis is a Gram-negative diplococcus since it has an outer and inner membranes with a thin layer of peptidoglycan in between. (wikipedia.org)
  • Neisseria meningitidis is an encapsulated Gram-negative diplococcus that asymptomatically colonises the upper respiratory tract in up to 25% of the population (mainly adolescents and young adults). (biomedcentral.com)
Lactamica2
  • Evidence of horizontal gene transfer (HGT) was observed in four individuals cocolonized by Neisseria lactamica and Neisseria meningitidis One HGT event resulted in the acquisition of 26 consecutive N. lactamica alleles. (ox.ac.uk)
  • With the exception of one 4 bp insertion, which was unique to a gonococcal isolate, all of the individual sequence changes were present in the N. lactamica isolates examined. (ox.ac.uk)
Serogroups5
  • Vaccines help protect against all three serogroups (B, C, and Y) of Neisseria meningitidis bacteria most commonly seen in the United States. (bizzieme.com)
  • Serogroups B, C, W and Y were investigated by PCR in 85 isolates recovered from the pharynx belonging to the second period. (bvsalud.org)
  • Isolates of serogroups B and C were more heterogeneous with several antigenic formulae. (who.int)
  • 1 ). Invasive meningococcal infections where isolates of serogroups A and W and typing of the isolates are all part of can occur in different clinical forms. (who.int)
  • Background: Expression of a capsule from one of serogroups A, B, C, W, X or Y is usually required for Neisseria meningitidis ( Nme ) to cause invasive meningococcal disease. (ox.ac.uk)
Recombination4
  • Conclusions: Data are consistent with the en bloc acquisition of cps in meningococci from N. subflava , followed by further recombination events with other Neisseria species. (ox.ac.uk)
  • Recombination within N.meningitidis strains and between Neisseria species was identified in SimPlot analysis. (biomedcentral.com)
  • The influence of mutation, recombination, population history, and selection on patterns of genetic diversity in Neisseria meningitidis. (ox.ac.uk)
  • A coalescent-based approach was employed to estimate the rate of mutation, the rate of recombination, and the size distribution of recombination fragments from samples from disease-associated and carried meningococci obtained in the Czech Republic in 1993 and a global collection of disease-associated isolates collected globally from 1937 to 1996. (ox.ac.uk)
Susceptibility2
  • The serogroup distribution, antibiotic susceptibility and antigenic and molecular characteristics of N. meningitidis isolates were determined in patients aged 3 days-13 years between February 1998 and June 2013. (who.int)
  • The countries, serogroup A and B isolates are Antibiotic susceptibility was performed case-fatality rate of invasive disease is predominant. (who.int)
Ampicillin1
  • Neisseria meningitidis sensitive to chloramphenicol and ampicillin has been isolated in 2 samples. (health.gov.au)
Disease isolates3
  • These assays were based on serogroup B disease isolates. (pubmlst.org)
  • Distribution of Bexsero ® Antigen Sequence Types (BASTs) in invasive meningococcal disease isolates: Implications for immunisation. (pubmlst.org)
  • The majority of opa genes in UK disease isolates (315/463, 68.0%) were in the 'on' phase, suggesting the importance of Opa proteins during invasive disease. (ox.ac.uk)
Antigens3
  • isolate contains antigens for which there is insufficient data from or are yet to be tested in experimental studies. (pubmlst.org)
  • In this study, we characterized invasive meningococcal isolates (n=294) recovered in Lithuania from 2009 to 2019 by multilocus sequence typing (MLST) and typing of antigens FetA and PorA. (nvspl.lt)
  • The more recent (2017-2019) serogroup B isolates (n=60) were genotyped by analyzing vaccine-related antigens to evaluate their coverage by fourcomponent (4CMenB) and two-component (MenB-Fhbp) vaccines using the genetic Meningococcal Antigen Typing System (gMATS) and Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index methods, respectively. (nvspl.lt)
Genomic1
  • Protein expression - We have not inferred from genomic data, therefore there may be isolates that possess genes but do no express the protein in vivo . (pubmlst.org)
Sequence5
  • We describe genomes of 2 of these N. meningitidfiis sequence type 11 complex urethritis tive for gonococcus by specific nucleic acid amplification isolates. (cdc.gov)
  • isolate contains ≥1 exact sequence match to antigenic variants found in the vaccine. (pubmlst.org)
  • One hundred four serogroup A meningococci in our collection, isolated in Africa from 1988 to 1999, were characterized by multilocus sequence typing (MLST). (cdc.gov)
  • A rare sequence type, ST-6697, predominated in disease specimens and isolates that were carried (n = 8/14), persisting for at least 44 months, likely driven by the high population density of houses (n = 67/112) and trailers (n = 45/112). (ox.ac.uk)
  • The complete sequence of gyrA gene from 77 strains are reported in this study and analyzed in the context of publicly available sequences from N. meningitidis of other CCs as well as other Neisseria species. (biomedcentral.com)
Susceptible2
  • Neisseria meningitidis isolates in the United States have been largely susceptible to the antibiotics recommended for treatment and prophylaxis. (cdc.gov)
  • N. meningitidis is transmitted from person to person, and close contact with a healthy carrier can facilitate the spread of the bacteria to susceptible individuals. (bmj.com)
Epidemic1
  • The PFGE technique is a relatively rapid and sensitive method for establishing clonal relationships among epidemic strains of N. meningitidis. (ox.ac.uk)
Outer membrane1
  • citation needed] Lipooligosaccharide (LOS) is a component of the outer membrane of N. meningitidis. (wikipedia.org)
Species5
  • These species usu- 31 years) in 3 gonoccocal treatment trials from April 2014 ally occupy distinct niches but may cause reciprocal diseases through February 2016 at the Bell Flower Clinic, a public when N. meningitidis colonizes the anogenital tract or gono- sexually transmitted infections clinic, in Indianapolis, Mar- coccus colonizes oropharyngeal mucous membranes ( 1 , 2 ). (cdc.gov)
  • rt-PCR for confirmation of isolate species and capsular genogrouping. (cdc.gov)
  • Following identification of putative capsule genes in non-pathogenic Neisseria species, this hypothesis is re-examined. (ox.ac.uk)
  • In the cps of 23/39 isolates, the two copies of region D were highly divergent, with rfbABC' sequences being more closely related to predicted orthologues in the proposed species N. weixii (GenBank accession number CP023429.1 ) than the same genes in Nme isolates lacking a capsule. (ox.ac.uk)
  • Some clusters contain sequences from other Neisseria species. (biomedcentral.com)
Meningococcus1
  • The potential of this technique for the rapid establishment of the clonal relationships between different isolates of the meningococcus was investigated. (ox.ac.uk)
Meningococci1
  • These data demonstrated that a substantial proportion of carried meningococci were incapable of capsule production, that the cnl circulated within Neisseria populations by horizontal genetic exchange, and that the expression of a polysaccharide capsule was not a requirement for person-to-person transmission of certain meningococcal lineages. (ox.ac.uk)
Nasopharynx2
  • N. meningitidis usually is transmitted through close contact with aerosols or secretions from the human nasopharynx. (bizzieme.com)
  • N. meningitidis is a part of the normal nonpathogenic flora in the nasopharynx of up to 8-25% of adults. (wikipedia.org)
Molecular1
PubMLST1
  • So far, 48 CCs have been described ( https://pubmlst.org/neisseria/ ). (biomedcentral.com)
Carbohydrate utilization2
  • N. meningitidis can be identified using Kovac's oxidase test and carbohydrate utilization. (bizzieme.com)
  • If the carbohydrate utilization test indicates that the isolate may be N. (bizzieme.com)
Diplococci2
  • N. meningitidis, like all neisseriae, is oxidase-positive and appears in a Gram stain as Gram-negative diplococci. (firebaseapp.com)
  • Gram stain of spun CSF showing N. meningitidis diplococci. (firebaseapp.com)
Specimens2
  • A pharyngeal swab from 1 of these men also lococci (GNID) identified by Gram stain of urethral exu- grew N. meningitidis , whereas rectal swab specimens from dates. (cdc.gov)
  • Please use a test request form (external link) to submit a specimens or bacterial isolate to ESR. (cri.nz)
Epidemiologic1
  • Meningococcal disease cases are reported through the National Notifiable Diseases Surveillance System, with additional epidemiologic information and isolates obtained through Enhanced Meningococcal Disease Surveillance. (medscape.com)
Intracellular1
  • 10 ). These cases have typically presented with purulent mented glucose and maltose but not lactose, consistent with urethritis or proctitis with gram-negative intracellular dip- N. meningitidis . (cdc.gov)
Pharyngeal1
  • Forty-three viable N. meningitidis isolates were cultivated (42 from pharyngeal and 1 from rectal swabs). (bmj.com)
Vaccine3
  • isolate contains ≥1 antigenic variant deemed cross-reactive to vaccine variants through experimental studies. (pubmlst.org)
  • Most serogroup B isolates (87.9%) were covered by a single vaccine antigen, most commonly Fhbp peptide variant 1 (84.5% of isolates). (nvspl.lt)
  • In total, 88.1% (CI 77.5-94.1) of isolates were predicted to be covered by the MenB-Fhbp vaccine. (nvspl.lt)
20161
  • The aim of this study was to assess the evolution of the DNA gyrase A ( gyrA ) gene from N.meningitidis CC4821 strains collected in China between 1978 and 2016. (biomedcentral.com)