Isolates in clinical. Medical search. Frequent questions

The contribution of the mucoid phenotype to pathogenesis has been demonstrated in several models: in general, the mucoid isolates are more resistant to phagocytosis, less sensitive to serum killing and more virulent in animal studies. (scirp.org)
Tuberculosis (TB) is one of the major causes of death related to antimicrobial resistance worldwide because of the spread of Mycobacterium tuberculosis multi- and extensively drug resistant (multi-drug resistant (MDR) and extensively drug-resistant (XDR), respectively) clinical isolates. (frontiersin.org)
To this aim, in this work an in vitro generation of M. tuberculosis mutants resistant to BDQ was performed starting from two MDR clinical isolates as parental cultures. (frontiersin.org)
Furthermore, several M. tuberculosis BDQ resistant mutants were isolated by both MDR strains, harboring mutations in both atpE and Rv0678 genes. (frontiersin.org)
These BDQ resistant mutants were further characterized by studying their growth rate that could be related to their spreading in clinical settings. (frontiersin.org)
The multidrug-resistant pools were genotypically similar to SMS-3-5, a previously sequenced multidrug-resistant isolate from a polluted environment. (datadryad.org)
The appearance of isolates with decreased susceptibility or resistant to third-generation cephalosporins (TGC) has raised several concerns. (bmj.com)
All CPE isolates carrying either bla OXA-232 or bla NDM-1 were resistant to meropenem, but only 40 from 45 were susceptible to colistin. (biomedcentral.com)
The percentage of isolates resistant to tetracycline, ciprofloxacin, penicillin, or all three antimicrobials, was greater in isolates from MSM than from MSW. (cdc.gov)
DNA polymorphisms were also determined by PCR-SSCP in isolates resistant to INH and RIF. (unesp.br)
BACTEC (TM) MGIT (TM) 960 detected 22 susceptible isolates to INH and RIF, 48 MDR isolates (resistant at least to INH and RIF) and nine mono-resistant isolates (eight to INH and one to RIF). (unesp.br)
PCR-SSCP detected DNA polymorphisms in 87.5% and 75.5% of isolates classified as INH-resistant and RIF-resistant, respectively. (unesp.br)
Sixty-seven of the 110 isolates (60.9%) were resistant to carbapenems, 80.60% (54/67) of which carried the bla OXA-23 gene. (frontiersin.org)
ABSTRACT A 21-year survey conducted in northeast Thailand of antimicrobial resistance to parenteral antimicrobial drugs used to treat melioidosis identified 24/4,021 (0.6%) patients with one or more isolates resistant to ceftazidime ( n = 8), amoxicillin-clavulanic acid ( n = 4), or both drugs ( n = 12). (tropmedres.ac)
Health facilities in the state should institute effective antimicrobial stewardship, intensify surveillance and screening of Staphylococcus aureus for MRSA strains to guard against dissemination of multidrug resistant strains in both hospital and community settings because of the clinical implications. (who.int)
resistant S. aureus (VRSA) in clinical samples The study was approved by the Health Ethics impacted negatively on the efficacy of vancomycin Committee of the State Ministry of Health and the 6 leading to treatment failures. (who.int)
The isolates were completely resistant to the second- and third-generation cephalosporins tested as well as carbapenems. (who.int)
The first carbapenem-resistant NDM-1 isolates characterized in 2009 were Klebsiella pneumoniae and Escherichia coli isolated from a Swedish patient who had sought medical care in New Delhi, India. (who.int)
All isolates were sensitive to third generation cephalosporin and floroquinolones but majority were resistant to ampicillin . (bvsalud.org)

The percentage of isolates with elevated cefixime MICs increased during 2009-2010, then decreased during 2012-2013 after treatment recommendations were changed in 2010 to recommend dual therapy (with a cephalosporin and a second antibiotic) and a higher dosage of ceftriaxone. (cdc.gov)
During that period, the percentage of isolates with elevated minimum inhibitory concentrations (MICs) to cephalosporins (≥0.25 µ g/mL for cefixime and ≥0.125 µ g/mL for ceftriaxone) increased from 0.2% in 2000 to 1.4% in 2010 for cefixime and from 0.1% in 2000 to 0.3% in 2010 for ceftriaxone. (cdc.gov)
Because few isolates exhibited decreased susceptibility and increases in MICs can precede the emergence of resistance, the percentage of isolates with elevated MICs (≥0.25 µ g/mL for cefixime and ≥0.125 µ g/mL for ceftriaxone) was assessed to determine if MICs to cephalosporins were increasing with time. (cdc.gov)

The Gonococcal Isolate Surveillance Project (GISP) was established in 1986 as a sentinel surveillance system to monitor trends in antimicrobial susceptibilities of N. gonorrhoeae strains in the United States. (cdc.gov)
This report summarizes trends in cephalosporin susceptibility among N. gonorrhoeae isolates in the United States during 2000--2010 using data from the Gonococcal Isolate Surveillance Project (GISP). (cdc.gov)

2005). Different types of electrophoretic techniques have been used for the characterization or typing of C. albicans isolates including separation of chromosomes, DNA fragments, isoenzymes, cell wall glycoproteins and whole cell proteins (Asakura et al . (scialert.net)
Molecular characterization of pathogenicity locus (PaLoc) and tcdC genetic diversity among tcdABClostridioides difficile clinical isolates in Tehran, Iran. (physiciansweekly.com)
Having previously disclosed the green synthesis and preliminary characterization of the oligomers [linear oligo(ethylenimine) hydrochloride and oligo(2-methyl-2-oxazoline) quaternized with N,N -dimethyldodecylamine] we herein report on the screening of these oligomers against a battery of 69 clinical isolates of Aerococcus spp. (springeropen.com)
In this study, we provide the molecular characterization and epidemiology for 12 NDM-1 positive clinical isolates. (who.int)

To investigate whether MinION-only sequencing is sufficient for identification, genome sequencing and downstream analysis in resource-limited settings, we sequenced the genomes of fourteen clinical isolates suspected to be Neisseria gonorrhoeae from Nairobi, Kenya. (nanoporetech.com)
Neisseria meningitidis isolates in the United States have been largely susceptible to the antibiotics recommended for treatment and prophylaxis. (cdc.gov)

The isolated yeasts were: Candida albicans (sixty-two isolates), Candida tropicalis (four isolates), Candida glabrata (two isolates), Candida parapsilosis (two isolates), Candida krusei (one isolate) and Trichosporon pullulans (one isolate). (scielo.br)
Effect of Probiotic Lactobacillus plantarum on Streptococcus mutans and Candida albicans Clinical Isolates from Children with Early Childhood Caries. (bvsalud.org)

In the present study the ability of clinical isolates of Salmonella enterica serovars Typhi (n = 30) and Paratyphi A (n = 11) to form biofilm on abiotic surface was investigated. (banglajol.info)
Onyango D, Machioni F, Kakai R, Waindi EN (2008) Multidrug resistance of Salmonella enterica serovars Typhi and Typhimurium isolated from clinical samples at two rural hospitals in Western Kenya. (jidc.org)

To describe the epidemiological, phenotypical and genetic characteristics of clinical isolates carrying the optrA gene identified in antimicrobial resistance surveillance by the laboratory of the National Institute of Health of Colombia. (paho.org)
Objectives were: describe the epidemiological and antibiotic susceptibility profile to NG strains isolated. (bmj.com)
We analysed the clinical information, performed antimicrobial susceptibility testing, and conducted molecular epidemiological and genomic analyses on the isolates to identify patterns in the data. (biomedcentral.com)
Although it appears that the isolates are clonally unrelated, epidemiological links cannot be fully excluded without further research. (who.int)

The two M. tuberculosis MDR clinical isolates were firstly characterized by whole genome sequencing, finding the main mutations responsible for their MDR phenotype. (frontiersin.org)
Six isolates were selected to perform whole genome sequencing. (paho.org)
All the isolates analyzed by whole genome sequencing showed the resistance genes fexA, ermB , lsaA , tet(M) , tet(L) and dfrG in addition to optrA and were negative for other mechanisms of resistance to linezolid. (paho.org)
Furthermore, blood culture isolates from patients with multiple episodes were subjected to whole genome sequencing and multilocus sequence typing. (lu.se)
Whole genome sequences of these isolates were determined and analysed to compute bacterial multilocus sequence types and plasmid replicon types, infer phylogenetic relationships, and identify antimicrobial resistance and virulence genes. (biomedcentral.com)
A combination of variable-number tandem repeat (VNTR) analysis, PCR for qac genes, and whole-genome sequencing was used to type isolates and examine possible mechanisms of resistance. (uea.ac.uk)

Putative virulence factor expression by clinical and food isolates of Bacillus spp. (strath.ac.uk)
The distribution of these traits was assessed within further clinical, environmental and food isolates associated with the outbreak, and this comparison indicated that three distinct, but highly related strains may have been involved in this nationwide outbreak. (biomedcentral.com)
Given the widespread occurrence of L. monocytogenes , subtyping of clinical and food isolates is required to establish epidemiologic links during routine surveillance, outbreak investigations, and for source tracking. (biomedcentral.com)

In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae ( Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae ) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. (who.int)
The isolates appear clonally unrelated with MLST, revealing a diversity of clonal types among the Klebsiella pneumoniae and Escherichia coli isolates. (who.int)
The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)

Each month, N. gonorrhoeae isolates are collected from up to the first 25 men with gonococcal urethritis attending each of the participating sexually transmitted disease (STD) clinics at 27 sites. (cdc.gov)

Fifty C. difficile isolates with known RTs and carrying the tcdA and tcdB toxin genes were analyzed. (physiciansweekly.com)
While PCR primer studies revealed that many different Bacillus species contained DNA sequences encoding the hemolysin BL (HBL) enterotoxin complex and B. cereus enterotoxin T, not all of these isolates expressed these diarrheagenic genes after growth in reconstituted IMF. (strath.ac.uk)
In conclusion, our studies demonstrated that the combination of phenotypic method REMA, which allowed rapid detection of MDR-MTB with higher levels of sensitivity and specificity, with the genotypic method PCR-SSCP, which demonstrated high accuracy in the search of polymorphisms in the resistance genes, proved to be a useful strategy to study MDR-MTB clinical isolates from national reference center located in São Paulo city. (unesp.br)
A total of 110 clinical isolates of A. baumannii , collected in a recent 2-year period, were tested for carbapenem antibiotic susceptibility, followed by a molecular analysis of carbapenemase genes. (frontiersin.org)

A total of 62 clinical samples from ATBUTH were collected and screened for K. pneumoniae . (scirp.org)
Umar, U. , Anagor, S. , Aliyu, A. and Suleiman, A. (2016) Hypermucoviscosity in Clinical Isolates of Klebsiella pneumoniae Correlates with High Multiple Antibiotic Resistance (MAR) Index. (scirp.org)
We examined the clinical data from patients with CPE infections and their outcomes, concentrating on Klebsiella pneumoniae isolates. (biomedcentral.com)
Altogether, 88.9% (40/45) of the CPE isolates were K. pneumoniae . (biomedcentral.com)
The most abundant carbapenemase gene family in the K. pneumoniae isolates (33/39) was bla OXA-232 , with bla NDM-1 additionally identified in 19 of them. (biomedcentral.com)
In vitro activities of BMS-284756 against Chlamydia trachomatis and recent clinical isolates of Chlamydia pneumoniae. (suny.edu)
Malay S, Roblin PM, Reznik T, Kutlin A, Hammerschlag MR. In vitro activities of BMS-284756 against Chlamydia trachomatis and recent clinical isolates of Chlamydia pneumoniae. (suny.edu)
The in vitro activities of BMS-284756 (a novel des-fluoroquinolone), levofloxacin, moxifloxacin, and clarithromycin were tested against 5 strains of Chlamydia trachomatis and 20 isolates of Chlamydia pneumoniae. (suny.edu)
The MIC at which 90% of the isolates were inhibited and the minimal bactericidal concentration at which 90% of the isolates were killed by BMS-284756 for all isolates of C. pneumoniae and C. trachomatis was 0.015 microg/ml (range, 0.015 to 0.03 microg/ml). (suny.edu)
In vitro activity of garenoxacin against recent clinical isolates of Chlamydia pneumoniae. (suny.edu)
In vitro activities of gemifloxacin (SB 265805, LB20304) against recent clinical isolates of Chlamydia pneumoniae. (suny.edu)

we sequenced genomic DNA from pools of E. coli clinical isolates with similar antibiotic resistance phenotypes using SOLiD technology to uncover SNPs unanimously conserved in each pool. (datadryad.org)
Among the pools of clinical isolates, genomic variation was concentrated adjacent to previously reported inversion and duplication differences between the SMS-3-5 isolate and the drug-susceptible laboratory strain, DH10B. (datadryad.org)
This study has revealed the genomic features of colonising CPE isolates, focusing on antimicrobial resistance and virulence determinants. (biomedcentral.com)
Notably, these two isolates were found to harbor a 50 kbp putative mobile genomic island encoding translocation and efflux functions that has not been observed in other Listeria genomes. (biomedcentral.com)

Genetic relatedness of the isolates was characterized by multilocus sequence typing. (frontiersin.org)

Dr. Hamprecht is a clinical microbiologist at the Institute for Medical Microbiology, Immunology and Hygiene and professor for antibiotic resistance of gram-negative pathogens at the University of Cologne, Germany, and the German Centre for Infection Research (DZIF), also in Cologne. (cdc.gov)
Finally, we also constructed a data sheet including the mutations associated with BDQ resistance that could be useful for the early detection of BDQ-resistance in MDR/XDR patients with the purpose of a better management of antibiotic resistance in clinical settings. (frontiersin.org)
Test isolates showing resistance to any of the 2nd and 3rd generation cephalosporins were subjected to double disc synergy test studies. (scialert.net)
However, treatment has been compromised by the absence of routine antimicrobial susceptibility testing in clinical care and evolution of antimicrobial resistance to the antibiotics used to treat gonorrhea. (cdc.gov)
However, 11 meningococcal disease cases reported in the United States during 2019-2020 had isolates that contained a bla ROB-1 β-lactamase gene associated with penicillin resistance, as well as mutations associated with ciprofloxacin resistance. (cdc.gov)
State and local public health departments should promote maintenance of laboratory capability to culture N. gonorrhoeae to allow testing of isolates for cephalosporin resistance. (cdc.gov)

nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a and Related Species, Japanese hospital. (cdc.gov)
C. albicans has been isolated from various sources (3,20), and is considered to be the most pathogenic species and frequently isolated from oral diseases in immunocompromised individuals (13). (scielo.br)
Forty-seven strains representing 14 different Bacillus species isolated from clinical and food samples were grown in reconstituted infant milk formulae (IMF) and subsequently assessed for adherence to, invasion of, and cytotoxicity toward HEp-2 and Caco-2 cells. (strath.ac.uk)
However, eight isolates belonging to the species B. cereus, B. licheniformis, B. circulans, and B. megaterium were found to produce this enterotoxin after growth in reconstituted IMF when assessed with the B. cereus enterotoxin (diarrheal type) reversed passive latex agglutination (RPLA) kit. (strath.ac.uk)
It is concluded that several Bacillus species occurring occasionally in clinical specimens and food samples are of potential medical significance due to the expression of putative virulence factors. (strath.ac.uk)
This is a cross-sectional study within two hospital settings to determine in vitro antibiotic susceptibilities of Salmonella species isolated in blood, cerebral spinal fluid, pus and stool collected from in- and out-patients. (jidc.org)
We evaluated fosfomycin susceptibility by multiple methods with multiple strains and species of KPC-producing clinical isolates collected at a single center between 2008 and 2016. (ox.ac.uk)
Overall, 63% (60/96) of isolates were susceptible by E-Test, 70% (67/96) by DD, and 88% (84/96) by agar dilution.FosAwas detected in 80% (70/88) of previously sequenced isolates, with species-specific associations and alleles, andfosA-positive isolates were associated with higher MIC distributions. (ox.ac.uk)

Susceptibility profiling of the isolates indicated that 100% retained susceptibility to tigecycline while 11/12 (91.7%) were susceptible to colistin. (who.int)
These isolates were obtained as part of a hospital surveillance programme for carbapenem non-susceptible Enterobacteriaceae. (who.int)
During the period of August 2010-August 2011, 52 non-duplicate carbapenem non-susceptible clinical isolates from local hospitals were analysed. (who.int)

Methods Descriptive retrospective study of NG strains isolated during 2011 and 2012. (bmj.com)
We determined the susceptibility profile of 80 Mycobacterium tuberculosis (MTB) clinical isolates from Brazil against isoniazid (INH) and rifampicin (RIF) drugs by two phenotypic methods (Resazurin Microtiter Assay -REMA and BACTEC (TM) MGIT (TM) Mycobacterial Detection System). (unesp.br)
Accordingly, additional subtyping methods are required to better characterize outbreak isolates. (biomedcentral.com)
Our results indicate thatfosAinfluences the inaccuracy of susceptibility testing by methods readily available in a clinical laboratory when compared to agar dilution. (ox.ac.uk)
Three hundred clinical samples were collected from male and female in and out-patients of all ages and processed using standard bacteriological methods. (who.int)
Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. (bvsalud.org)

In patients with MDS with isolated del(5q), the morphologic dysplasia is most evident as an increase in the number of small, hypolobated megakaryocytes, as seen in the following image. (medscape.com)
The prognosis for patients with t-MDS is not as favorable as the prognosis for patients with MDS with isolated del(5q). (medscape.com)
Patients with MDS with isolated del(5q) typically present with persistent anemia that has no other identifiable cause. (medscape.com)
The aims were to study the clinical presentation of patients with EfsB with focus on the risk of recurrent infection and IE, identify potential improvements of the management, and to investigate whether E. faecalis isolates from different episodes in the same patient were identical. (lu.se)
In 28 of 31 patients with two or more EfsB episodes, isolates with identical sequence type were found. (lu.se)
Episodes of EfsB in patients later diagnosed with IE showed features of IE already during the first episodes, were not adequately evaluated, are due to identical isolates, and most likely represent true relapses. (lu.se)
In order to investigate yeasts in oropharyngeal secretion, urine, sputum and inguinal scales from AIDS patients, clinical samples were collected from one hundred patients interned in the Infectious and Parasitic Diseases Sector of the Hospital das Clínicas of the Universidade Federal de Pernambuco and in Hospital Universitário Osvaldo Cruz of the Universidade de Pernambuco. (scielo.br)
Considering the increasing number of cases of AIDS in Pernambuco, Brazil, and the susceptibility to opportunistic mycosis, the objective of this study was to isolate and identify yeasts in clinical samples of AIDS patients. (scielo.br)
From one hundred AIDS patients (75 male and 25 female), interned at the Sector of Infectious and Parasitic Diseases (DIP) of the Hospital das Clínicas (Universidade Federal de Pernambuco) and Hospital Universitário Osvaldo Cruz (Universidade de Pernambuco), 108 clinical samples (95 from the oropharyngeal secretion, 11 from urine, one sputum and one from inguinal scale) were collected. (scielo.br)
Results A total of 275 NG strains were isolated: 143 urethral (52%), 47 rectal (17%), 44 pharyngeal (16%), 28 endocervical (10%), vaginal and 13 (5%), belonging to 225 patients (174 men (77.3%), 51 (22.7%) women). (bmj.com)
The clinical characteristics of 33 hospitalised patients with confirmed CPE, including patient-related factors associated with the development of CPE infections, were examined. (biomedcentral.com)
Results: Autoantibody reactivities on the skin and also proliferative responsesto isolated autologous skin antigens were higher in patients than in controls. (medscimonit.com)
Pour identifier les espèces de Leishmania, des techniques moléculaires ont été appliquées sur des échantillons prélevés chez 64 patients atteints de leishmaniose cutanée orientés vers l'hôpital régional d'Herat en 2013. (who.int)
BestBets: In patients with isolated upper extremity injury, does the use of temporary immobilisation increase the short term risk of venous thromboembolic events? (bestbets.org)
We present evidence that isolates are evolving in the face of biocide challenge in patients and that changes in decolonization regimes are reflected in changes in susceptibility of isolates. (uea.ac.uk)
Administration of prophylactic antibiotics to patients requiring chest tube thoracostomy for isolated chest injuries reduces the incidence of intrathoracic infection especially empyema with an number needed to treat of six. (bmj.com)
Here, we isolated for the first time, DA storage vesicles from the striatum of six autopsied brains of PD patients and four controls and measured several indices of vesicular DA storage mechanisms. (jneurosci.org)
strains were isolated in 70% of the patients. (bvsalud.org)

No Azi-RS isolates exhibited reduced cefixime or ceftriaxone susceptibility (Cfx-RS and Cro-RS, respectively). (cdc.gov)
MICs to cephalosporins (cefixime and ceftriaxone) among gonococcal isolates collected during 2000--2010 were analyzed. (cdc.gov)

The percentage of isolates with ceftriaxone MICs ≥0.125 µ g/mL increased from 0.1% to 0.3% during 2000--2010 (p = 0.047). (cdc.gov)
Isolates with increased MICs and MBCs of octenidine rapidly emerged after octenidine was introduced in the trust. (uea.ac.uk)

The genetic environment of the optrA-2 (ST16) isolates presented the impB, fex, optrA segment, associated with plasmid, while in two isolates ( optrA-6 and optrA-5 ) the transferable chromosomal element Tn6674-like was found. (paho.org)
One CRAB isolate of ST195 harbored plasmid pAB52 from a Chinese patient without travel history. (frontiersin.org)

CPE isolates were purified, and antimicrobial susceptibility tests performed. (biomedcentral.com)
Isolates are tested for antimicrobial susceptibility using agar dilution at one of five regional laboratories. (cdc.gov)
Laboratories should be aware that CDC recommends that healthcare providers use antimicrobial susceptibility testing (AST) to determine susceptibility of all meningococcal isolates to penicillin before changing from empirical treatment with cefotaxime or ceftriaxone to penicillin or ampicillin. (cdc.gov)

Isolate relatedness was determined by multilocus sequence typing (MLST). (who.int)

Five representative ESBL positive isolates, one from each specimen, were investigated for their susceptibility patterns to different gentamicin and floroquinolone combinations. (scialert.net)
Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct Asc I PFGE patterns. (biomedcentral.com)

The optrA gene was confirmed in 23/25 isolates of E. faecalis from seven departments in Colombia. (paho.org)
nov., a novel Alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of Alcaligenes denitrificans Rüger and Tan 1983 as Achromobacter denitrificans comb. (microbiologyresearch.org)
Classification of Alcaligenes faecalis -like isolates from the environment and human clinical samples as Ralstonia gilardii sp. (microbiologyresearch.org)

Between October 2014 and February 2019, 25 isolates of Enterococcus spp . (paho.org)
To isolate and identify in a Chilean population, Enterococcus spp. (bvsalud.org)

The aim of the study was to evaluate the protein similarity degree among 15 C. albicans isolates with different virulence obtained from healthy and infected human and animals. (scialert.net)
In this study, we described the use of SDS-PAGE, along with PAGE techniques, to analyze the similarity of the protein profiles of the cytoplasmic extracts and cell walls of different C. albicans isolates obtained from different host conditions (human against animal isolates and patient against healthy subject isolates) with various virulence degrees. (scialert.net)
Seventeen fresh C. albicans , originated from infected human (7 isolates) and animals (10 isolates), were obtained from Fungal Collection of Mycology Research Center, University of Tehran between April and November, 2007. (scialert.net)
In this study, three probiotic Lactobacilli strains ( Lactobacillus plantarum 8014, L. plantarum 14917, and Lactobacillus salivarius 11741) were tested on S. mutans and C. albicans clinical isolates using a multispecies biofilm model simulating clinical cariogenic conditions. (bvsalud.org)
The ten pairs of clinical isolates of S. mutans and C. albicans were obtained from children with severe early childhood caries. (bvsalud.org)
Our study findings show a remarkable inhibitory effect of L. plantarum 14917 on S. mutans and C. albicans clinical isolates, resulting in significantly reduced growth of S. mutans and C. albicans, a compromised biofilm structure with a significantly smaller microbial and extracellular matrix and a less virulent microcolony structure. (bvsalud.org)

The medical record limits the information available on patient medical history and clinical characteristics. (bestbets.org)

All isolates were found capable of biofilm formation in a microtitre plate assay. (banglajol.info)
Upon optimization of biofilm formation by the test isolates, Adherence test medium (ATM) was found to be the best medium for biofilm formation by both S. enterica serovars Typhi and Paratyphi. (banglajol.info)
Of the 28 Salmonella Typhi isolates, 17 (61%) were very strong biofilm producers, 8 (29%) were strong biofilm producers and 3 (11%) were moderate biofilm producers. (banglajol.info)
The present study raises concern from a public health point of view because the ability of the clinical isolates to form biofilm would indicate their ability of being transmitted from abiotic surface to uninfected host giving rise to disease. (banglajol.info)

The susceptibility profile of gentamicin is similar to those published but further research is needed to establish clinical breakpoints and doing a treatment recommendation. (bmj.com)

This study was therefore designed to investigate the in vitro therapeutic outcome of combining an aminoglycoside (gentamicin) and some floroquinolones including ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin at different ratios against E. coli isolates expressing ESBL enzymes. (scialert.net)
The present studies were performed to investigate the possibility of culturing isolated pancreatic islets in vitro in a system which should allow assessment of both morphologic and metabolic alterations in the islet cells after prolonged periods of incubation. (diabetesjournals.org)
The results indicate that with the present technic for organ culture of isolated mouse islets, the β cells retain several of their characteristic metabolic responses during prolonged in vitro culture. (diabetesjournals.org)

European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Clostridioides Difficile (ESGCD). (physiciansweekly.com)

In this study, we assembled a panel of S. aureus isolates, including isolates collected before the development of chlorhexidine and octenidine and isolates, from a major hospital trust in the United Kingdom during a period when the decolonization regimes were altered. (uea.ac.uk)

None of the patient without clinical evidence of wound infection had bacterial positive wound swab culture . (bvsalud.org)

potentially pathogenic Talaromyces isolates, 2 T. marneffei . (cdc.gov)

Parents of children with isolated microscopic hematuria should be reassured that sufficient time remains to plan a stepwise evaluation. (medscape.com)

Clinical Infectious Diseases. (wikipedia.org)

This article aims at keeping an up-to-date list of Coronavirus strains and subspecies successfully isolated and cultured in laboratory, a task which is often challenging. (wikipedia.org)
However, establishing accurate fosfomycin susceptibility testing for non-E. coliin a clinical microbiology laboratory remains problematic. (ox.ac.uk)
Clinical Laboratory and Standards Institute (CLSI) breakpoints forE. (ox.ac.uk)
The laboratory tests ordered for the evaluation of hematuria must be based on the clinical history and the physical examination. (medscape.com)

A polyphasic taxonomic study was performed on nine isolates recovered from various human clinical samples. (microbiologyresearch.org)

One hundred and four clinical isolates of E. coli obtained from urine (26), blood (24), stool (20), sputum (19) and semen (14) were investigated for ESBL production. (scialert.net)
The isolates were obtained from urine (26), blood (24), stool (20), sputum (19) and serum (14). (scialert.net)

High incidence of group C streptococci isolated from throat swabs. (bmj.com)

High-throughput pyrosequencing of two L. monocytogenes isolates was used to rapidly provide the genome sequence of the primary outbreak strain and to investigate the extent of genetic diversity associated with a change of a single restriction enzyme fragment during PFGE. (biomedcentral.com)

The effects of interleukin 1 (IL-1) on glucose-induced insulin secretion from isolated rat islets of Langerhans have been examined. (diabetesjournals.org)
For this purpose, isolated islets from adult mice were maintained in stationary organ cultures in a nutrient medium containing glucose at a concentration of either 1.2 mg./ml. or 5 mg./ml. (diabetesjournals.org)
The metabolic rates were determined in short-term incubations (30-150 minutes) so that relevant comparisons with freshly isolated mouse islets could be performed. (diabetesjournals.org)
Similarly, the rates of oxygen consumption, glucose oxidation and insulin release responded responded to glucose throughout the culture period in a manner qualitatively similar to these responses in freshly isolated islets. (diabetesjournals.org)

Further research is needed to determine the impact offosAon clinical outcomes. (ox.ac.uk)

The present study evaluated the antimicrobial effect of different combinations of gentamicin and floroquinolones (ciprofloxacin, ofloxacin, perfloxacin and gatifloxacin) against extended spectrum beta lactamase (ESBL) positive E. coli isolates using checkerboard method. (scialert.net)
One hundred and four clinical isolates of E. coli were collected from the intensive care unit of Eastern Nigeria Medical Centre Enugu. (scialert.net)

OptrA -positive clinical isolates present a high genetic diversity, with different optrA clones and variants related to two types of structures and different mobile genetic elements. (paho.org)

Most of these CRAB isolates (77.62%) were classified as clone complex 92 (CC92), and sequence type (ST) 92 was the most prevalent STs, followed by ST195, ST136, ST843, and ST75. (frontiersin.org)

Anatomy4

Organisms52

Diseases33

Chemicals and Drugs64

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes49

Disciplines and Occupations7

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science5

Health Care14

Geographicals12

Resistant19

Percentage of isolates with elevated3

Gonococcal Isolate Surveillance Project2

Characterization4

Neisseria2

Candida2

Salmonella enterica Serovars Typhi2

Epidemiological4

Whole genome6

Food isolates3

Escherichia3

Gonorrhoeae isolates1

Genes4

Pneumoniae11

Genomic4

Characterized by multilocus1

Resistance6

Species8

Susceptible3

Methods6

Patients18

Cefixime2

MICs2

Plasmid2

Antimicrobial susceptibility3

Multilocus1

Patterns2

Faecalis3

Enterococcus2

Albicans6

Characteristics1

Biofilm4

Susceptibility profile1

Vitro3

Microbiology and Infectious Diseases1

Aureus1

Bacterial1

Pathogenic1

Microscopic hematuria1

Infectious Diseases1

Laboratory4

Study was performed1

Urine2

Swabs1

Monocytogenes1

Islets4

Outcomes1

Coli2

Genetic diversity1

Sequence1