Isolates form biofilms. Medical search. Frequent questions

In this study, the anti-biofilm activity of previously fabricated polyamino-phenolic ligands and polyamidoamine dendrimers was investigated against legionella mono-species and multi-species biofilms formed by L. pneumophila in association with other bacteria that can be found in tap water ( Aeromonas hydrophila , Pseudomonas aeruginosa , Escherichia coli , Klebsiella pneumoniae ). (frontiersin.org)
Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
Extreme spaceflight conditions can force these bacteria to toughen up, while simultaneously lowering the immune defenses of the stressed, isolated crew. (frontiersin.org)
isolates, while the role of cell-free supernatants, from diverse bacteria, in inducing enhanced adherence was investigated using microtitre plate assays. (biomedcentral.com)
Bacteria biofilms are increasingly recognized as contributing to bacterial persistence and disease pathogenesis in CF. (nih.gov)
1,2 The unique structure of biofilms protects bacteria from the surrounding environment, conferring a capacity for persistence against phagocytosis, oxidative stresses, nutrient/oxygen restriction, metabolic waste accumulation, interspecies competition, and conventional antimicrobial agents. (rsc.org)
2,9-11 A comprehensive understanding of the interactions between bacteria and materials with different surface topographies may pave the way for more effective strategies to control biofilm growth. (rsc.org)
Instead, the cells take on different roles, including the initial free-swimming bacteria, the scaffolding-producing cells, and, eventually, a third type of cell that send out spores to start new biofilms. (harvard.edu)
By forming a wide network with the scaffolding, known as the extracellular matrix, the bacteria can collect nutrients from a larger area. (harvard.edu)
Moreover, in mixed-species biofilms, the waste products of one type of bacteria can be used by another species. (harvard.edu)
In addition, the diversity of bacteria in a colony can make it difficult for a single treatment to destroy the biofilm. (harvard.edu)
This technique favored organisms that could grow well floating in liquid, but could not detect the diverse congregations of bacteria that would typically form on surfaces. (harvard.edu)
The thesis elucidates the multi-metal resistance potential, biofilm-forming ability, and enhanced heavy metal removal efficiency of bacteria isolated from metal contaminated sites. (nitrkl.ac.in)
From the results obtained, it can be concluded that food is a source of coagulase-negative staphylococci capable of forming biofilm, which is referred to as clinically important virulent factor of these bacteria. (edu.pl)
Additional studies on the effective elimination of biofilms containing multidrug-resistant bacteria are necessary, and we hope that a treatment method superior to sterilization with antibiotics will be developed in the future. (ophrp.org)
A study examining the biofilm-forming ability of multidrug-resistant A. baumannii strains recently isolated from a clinical sample indicated that all resistant bacteria exhibited higher biofilm-forming ability than the standard A. baumannii strain ATCC 19606 [ 11 ]. (ophrp.org)
Bacteria with high biofilm-forming ability attach to host cells more easily or colonize medical devices and hospital environments, promoting horizontal gene transfer between bacteria in biofilms [ 14 , 15 ]. (ophrp.org)
In the presence of the efflux inhibitor phe-arg- β-naphthylamide (PAβN), wildtype bacteria and 36/86 non-replicate clinical isolates of carbapenem-producing Enterobacteriaceae were ≥4-fold less susceptible to ertapenem. (bham.ac.uk)
Although it is well established that pathogenic bacteria form biofilms extensively in nature to survive and resist adverse conditions by attaching to each other and to a substrate with extracellular polymeric substance (EPS). (lincoln.ac.uk)
There was no significant difference observed in the density of biofilms formed by isolates from different sources but out of the 9 isolates tested previously for the presence of adhesin genes (virulence factors/appendages that facilitates bacteria attachment to host cell/surfaces), moderate biofilm producers harbored multiple adhesin genes whilst weak biofilm producers harbored only one adhesin gene each. (lincoln.ac.uk)
The vast majority of the bacteria that surround us are not free-floating but prefer to band together in cooperative communities called biofilms. (theconversation.com)
The "smoking gun," he said, is DNA analysis proving that the bacteria isolated from sick people and the likely source are identical. (cdc.gov)
Because of the potential to form biofilm, CDC recommends that all dental unit waterlines be treated regularly with disinfectants to meet the Environmental Protection Agency (EPA) regulatory standards for drinking water (i.e., ≤500 colony forming units (CFU)/mL of heterotrophic water bacteria). (cdc.gov)
This bacteria is most frequently isolated in CAP cases after endodontic tooth treatment, but it may also be found in cases of necrotic pulps in very few cases 12 . (bvsalud.org)

The concentration of the chemicals tested as anti-biofilm agents was chosen based on cytotoxicity assays: the highest non-cytotoxic chemical concentration was used for biofilm inhibition assays, with dendrimer concentration 10-fold higher than polyamino-phenolic ligands. (frontiersin.org)
Partial inhibition of coaggregation was observed when isolate CH2B was treated with heat or protease exposure, suggesting the presence of heat-sensitive adhesins, although sugar treatment resulted in increased coaggregation and may be associated with a lactose-associated lectin or capsule-mediated attachment. (biomedcentral.com)
In this study the effectiveness of chlorine, ozone and hydrogen peroxide (H 2 O 2 ) in the inhibition of mature biofilms or biofilm formation in natural seawater was investigated. (scielo.org.za)
The effects of these NPs on Acinetobacter baumannii , Staphylococcus aureus , and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, -scanning electron microscopy, and atomic force microscopy. (dovepress.com)
AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus . (dovepress.com)
This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. (dovepress.com)
In this study, we demonstrate that octanoic acid has broad-spectrum microbicidal activity against MCPs and effectively inhibits S. aureus biofilm formation in milk (>50% inhibition at 3.13 mM). (ntu.edu.tw)
This study aimed to test the effect of photodynamic therapy (PDT) on the inhibition and removal of biofilms containing multidrug-resistant Acinetobacter baumannii . (ophrp.org)
Using the viable count method to determine the effect of treatment showed minimal (2%/0.1 log) to good (28% /2.5 log) biofilm inhibition and reduction. (lincoln.ac.uk)
Additionally, we performed hyphae inhibition and biofilm tests. (who.int)
E demonstrated no effective action against biofilms, though SE showed inhibition against biofilms of all strains. (who.int)

E. meningoseptica isolate CH2B and strain NCTC 10016 T displayed a strong biofilm-forming phenotype which may play a role in its potential pathogenicity in both clinical and aquaculture environments. (biomedcentral.com)
The biofilm screening of 58 isolates exhibited strong biofilm formation by 17 strains, moderate biofilm formation by 15 strains, weak biofilm formation by 21 strains, and no biofilm formation by 5 strains. (nitrkl.ac.in)
Out of the 17 strong biofilm former, 8 bacterial strains exhibited tolerance to high concentrations of Cr, Pb, and Cd, i.e., ˃500 mg/L. The potent multi-metal resistant biofilm-forming bacterial strains were identified as Pseudomonas aeruginosa OMCS-1, Staphylococcus sp. (nitrkl.ac.in)
Logistic regression was used to quantify the effect of strong biofilm production (versus weak biofilm production) on clinical outcomes and independent predictors of a strong biofilm. (uri.edu)
95% CI, 0.002 to 0.39) were less likely to harbor strong biofilm-producing MRSA. (uri.edu)
7/54 (13%) were strong biofilm producers, 31/54 (57%) were moderate biofilm producers and 16/54 (30%) were weak biofilm producers according to current criteria. (lincoln.ac.uk)

Role of a new filamentous prophage Pf4 in Pseudomonas aeruginosa biofilm development. (southampton.ac.uk)
We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
This project will exploit novel nitric oxide based therapies to improve the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. (southampton.ac.uk)
Multidrug-resistant Pseudomonas aeruginosa poses a global challenge due to its virulence and biofilm-forming ability, leading to persistent infections. (researchgate.net)
This study had a dual focus: first, it aimed to investigate the biofilm activity and antibiotic resistance profiles of Pseudomonas aeruginosa isolates obtained from a fish-rearing farm. (researchgate.net)
Here, we investigated bacterial attachment, cell alignment and biofilm formation of Pseudomonas aeruginosa on periodic nano-pillar surfaces with different pillar spacing. (rsc.org)
4,5 For example, Pseudomonas aeruginosa , an organism well-known for its capacity to form biofilms, is an opportunistic pathogen and is one of the top three causes of opportunistic human infections, 6 causing nosocomial infections in catheter lines, or chronically infecting the lungs of cystic fibrosis patients. (rsc.org)
The adsorption behavior and interaction mechanisms of extracellular polymeric substances (EPS) of a biofilm-forming bacterium, Pseudomonas aeruginosa OMCS-1, towards Cr, Pb, and Cd were investigated. (nitrkl.ac.in)

Umar, U. , Anagor, S. , Aliyu, A. and Suleiman, A. (2016) Hypermucoviscosity in Clinical Isolates of Klebsiella pneumoniae Correlates with High Multiple Antibiotic Resistance (MAR) Index. (scirp.org)
Comparative sequence analysis of the rpoB gene was performed coupled with phenotypic characterization of antibiotic resistance, motility, biofilm production and clinical correlation. (biomedcentral.com)
Failure of antibiotic treatments is often attributed to formation of bacterial biofilms and abscesses in the mammary gland tissue, which lead to chronic infections that are difficult to eradicate and drive recurrent disease. (ntu.edu.tw)
Additionally, octanoic acid rapidly kills persistent biofilm cells and cells with antibiotic tolerance (within 4 h). (ntu.edu.tw)
In contrast, antibiotics treated at >100X MBC cannot eradicate biofilms but do induce SCVs and antibiotic-tolerant cells. (ntu.edu.tw)
Thus, octanoic acid exhibits bactericidal action against S. aureus biofilms, and it is less likely than antibiotic therapy to induce persistent cells and pathogen tolerance. (ntu.edu.tw)
Using multidrug-resistant A. baumannii strains, an antibiotic susceptibility test was performed using the Gram-negative identification card of the Vitek 2 system (bioMérieux Inc., France), as well as an analysis of resistance genes, the effects of treatment with a light-emitting diode (LED) array using Radachlorin (RADA-PHARMA Co., Ltd., Russia), and transmission and scanning electron microscopy to confirm the biofilm-inhibitory effect of PDT. (ophrp.org)
Combination of antibiotic and nanoparticles improved biofilm reduction compared to antibiotic alone, but this was not generally significant at the concentration tested. (lincoln.ac.uk)

The ability of Elizabethkingia meningoseptica isolate CH2B from freshwater tilapia ( Oreochromis mossambicus ) and E. meningoseptica strain NCTC 10016 T to adhere to abiotic surfaces was investigated using microtiter plate adherence assays following exposure to varying physico-chemical challenges. (biomedcentral.com)
Biofilm assays showed that the O-:H4 strain, but none of the serotype O157:H7 strains, bound Congo red dye and formed a biofilm. (usda.gov)
One of the objectives of this study was to compare the biofilm-forming potential of C. perfringens isolates from broiler chickens, free-range poultry environments and neonatal humans using the traditional protocols of crystal violet staining assays in microtiter plates. (lincoln.ac.uk)
The fitness of each species was evaluated through motility, biofilm, desiccation and benzalkonium chloride (BAC) assays. (dal.ca)

Common bacterial isolates cultured from patients with keratitis include P aeruginosa , coagulase-negative staphylococci, Staphylococcus aureus , Streptococcus pneumoniae, and Enterobacteriaceae (including Klebsiella, Enterobacter, Serratia, and Proteus ). (medscape.com)
Nevertheless, all bacterial isolates were able to form immunity-evading slimy coatings, and most were resistant to at least three antibiotics. (frontiersin.org)
Using bacterial cultivation methods, bacterial isolates belonging to the genus Enterococcus were identified. (bvsalud.org)

Coaggregation by isolate CH2B appeared to be strongest with foodborne pathogens like Enterococcus, Staphylococcus and Listeria spp. (biomedcentral.com)
Anguka J. Effects of antimicrobials in vitro on Staphylococcus saprophyticus in planktonic and biofilm forms isolated from patients with urinary tract infections J. Environ. (pulsus.com)
Clinical and genetic risk factors for biofilm-forming Staphylococcus a" by Megan Luther, M. Parente et al. (uri.edu)
The molecular and clinical factors associated with biofilm-forming methicillin-resistant Staphylococcus aureus (MRSA) are incompletely understood. (uri.edu)
A major mastitis-causing pathogen (MCP) associated with biofilms in bovine mastitis is Staphylococcus aureus. (ntu.edu.tw)

Nocardioides salsibiostraticola is a Gram-positive, aerobic, non-spore-forming and non-motile bacterium from the genus Nocardioides which has been isolated from biofilm from coastal seawater from the Norwegian Sea. (wikipedia.org)
Legionnaires' disease is a potentially fatal pneumonia caused by Legionella pneumophila , an aquatic bacterium often found within the biofilm niche. (frontiersin.org)
An interesting characteristic of this bacterium is its ability to form biofilms that display complex heterogeneity: individual, clonal cells develop diverse phenotypes in response to different environmental conditions within the biofilm. (nature.com)
strain PV-1 is a piezophilic, thermophilic, anaerobic, chemolithoautotrophic bacterium isolated from a deep-sea hydrothermal vent on the East Pacific Rise. (rutgers.edu)
Varunaivibrio sulfuroxidans is a mesophilic, facultative anaerobic, facultative chemolithoautotrophic bacterium isolated from a sulfidic shallow-water marine gas vent located at Tor Caldara, in the Tyrrhenian Sea, Italy. (rutgers.edu)
Sulfurovum riftiae is a mesophilic, anaerobic chemosynthetic bacterium isolated from the tube of the vent polychaete, Riftia pachyptila on the East Pacific Rise. (rutgers.edu)
Cetia pacifica is a thermophilic, anaerobic chemosynthetic bacterium isolated from an active deep-sea hydrothermal vent on the East Pacific Rise. (rutgers.edu)
Galenea microaerophila is a mesophilic, strictly microaerophilic, chemosynthetic bacterium isolated from the sediment of an active shallow-water hydrothermal vent in Paleochori Bay, on the Greek island of Milos. (rutgers.edu)
Parvibaculum hydrocarbonoclasticum is an aerobic, alkane-oxidizing bacterium isolated from hydrothermal fluids collected from a deep-sea vent on the East Pacific Rise. (rutgers.edu)
Phorcysia thermohydrogeniphila is a hyperthermophilic, anaerobic chemolithotrophic bacterium isolated from a tube of an Alvinella pompejana tubeworm collected at the East Pacific Rise at 13N. (rutgers.edu)
Fitness studies measuring growth rates, ability to form biofilm, conjugation frequency and plasmid persistence showed that both plasmids affected the host bacterium but in different ways. (bham.ac.uk)

Chlorination and UV are commonly used for disinfection of this water but may not be effective in preventing biofilm formation within the water distribution network. (scielo.org.za)

The vast majority actually live in highly organized communities called biofilms, which can contain many different species. (harvard.edu)

This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)
3 Bacterial biofilms can cause persistent human infections and can foul the surface of medical devices. (rsc.org)

DNA finger- be especially important for C. parapsilosis , since printing indicated that the outbreak isolates were clonal indwelling devices appear to be the predominant route of and genetically distinct from our database. (cdc.gov)
Six of the outbreak isolates were isolated from either produce or from patients that consumed the contaminated produce, and all were serotype O157:H7, the most common STEC serotype associated with foodborne outbreaks. (usda.gov)

Sequencing of the Shiga toxin genes of the six serotype O157:H7 isolates from the two different outbreaks showed that all of the strains were 100% identical. (usda.gov)
Sixteen of the isolates examined were serotype O157:H7, and all were positive by PCR for hly, eae, wyzO157, and either stx1, stx2 or both toxin genes. (usda.gov)
An E. coli serotype O-:H4 strain isolated from a spinach bag during the spinach-associated outbreak was negative for all tested virulence genes except for stx1. (usda.gov)
Sequencing of the Shiga toxin genes from serotype O157:H7 isolates associated with the two 2006 outbreaks revealed that all contained both a stx2 and a stx2c variant gene but not stx1. (usda.gov)
The two variants exhibit different capability to form biofilms and to express genes involved in polysaccharide biosynthesis, flagellar synthesis, and the AI-2 quorum-sensing system [ 18 ]. (hindawi.com)
The aim of the study was to analyze the biofilm production capacity of coagulase-negative staphylococci (CNS) isolated from ready-to-eat meat products including pork ham, chicken cold cuts, pork sausage, salami and pork luncheon meat, sliced in the store to the consumer's specifications, along with determination of occurrence of the adhesion genes determining the polysaccharide production (icaA and icaD) and collagen binding protein (cna). (edu.pl)
The assessment of biofilm production capacity by staphylococci was made using crystal violet, whereas evaluation of the frequency of occurrence of genes was performed using the PCR. (edu.pl)
and 22.7 percent of the 720 E. coli isolates carried genes for EAEC (including EAST 1) [12]. (i-sis.org.uk)
Over 670 presumptive Listeria isolates collected from an urban and a rural Nova Scotia watershed were identified through sequencing of the 16S rRNA and/or sigB genes. (dal.ca)

Pilin expression is associated with auto-agglutination in liquid suspension or broth cultures, and appears to be necessary but not sufficient for expression of the agar-pitting phenotype and for the formation of biofilms. (microbiologyresearch.org)

This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)
Bacterial cells can colonize surfaces and form biofilms that consist of microbial cells embedded in extracellular polymeric substances (EPS). (rsc.org)

AB then compared adhesion ability, biofilm production, and where E is the number of bands common to both strains, a secretion of SAP and phospholipase B of the outbreak iso- is the number of bands unique to strain A, and b is the lates and our clinical strains. (cdc.gov)
However, when the serotype O-:H4 isolate and the serotype O157:H7 isolate that originated from the same spinach bag were cultured together in biofilm studies the serotype O157:H7 strain was able to form greater amounts of biofilm than the serotype O-:H4 isolate. (usda.gov)
The three strains were able to grow in the synthetic medium and remove phosphorus in percentages, between 6 and 43%, which varied between strains and also among each strain according to the biofilm growth phase. (torvergata.it)
Unlike the STECs commonly implicated in foodborne outbreaks, the current outbreak strain can form biofilms that help it cling to human intestines . (cdc.gov)

We determined the ability of Candida parapsilosis isolates from single fungaemia episodes to form biofilms and we analysed biofilm subgroups for antifungal susceptibility and pathogenic potential. (unicatt.it)
Antifungal susceptibility of preformed biofilms was assessed, and virulence was studied using the Galleria mellonella model. (unicatt.it)
These biofilms exhibited decreased susceptibility to antibiotics and were adherent to epithelial surfaces. (nih.gov)
NTHi formed adherent biofilms on the apical surface airway epithelia with decreased susceptibility to antibiotics, and respiratory cells exhibited inflammatory and host defense responses-evidence of a dynamic host-pathogen interaction. (nih.gov)
In addition, the susceptibility of C. perfringens to a range of veterinary or human antimicrobial drugs in conventional or biofilm modes were tested using the broth microdilution method. (lincoln.ac.uk)
In summary, the results in this thesis show that C. perfringens grown in biofilm mode independent of the source of isolation could reduce the effective susceptibility of antimicrobials. (lincoln.ac.uk)
Biofilm production by Trichosporon facilitates colonization of indwelling devices, permitting both adherence to prosthetic material and reduction of the fungus's exposure and susceptibility to antifungal drugs. (medscape.com)
in different drinking- water sources in Basra governorate as well as the susceptibility of isolates to several antibiotics. (who.int)

These data suggest that the studied compounds, especially dendrimers, could be considered as novel molecules in the design of research projects aimed at the development of efficacious anti-biofilm disinfection treatments of water systems in order to minimize legionellosis outbreaks. (frontiersin.org)
Researches on the virulence factors of H. influenzae, particularly the mechanisms underlying biofilm formation, and the development of anti-biofilm strategies hold significant clinical value. (bvsalud.org)
Understanding its virulence factors, especially the formation mechanism of biofilm, and formulating effective anti-biofilm strategies may help to reduce the clinical impact. (bvsalud.org)

3,8 Therefore, it is important to develop biomaterials that can control biofilm growth thereby reduce infections. (rsc.org)
Furthermore, it showed that alternative therapeutic strategies have the potential to control biofilm related contamination and infections in animals and people. (lincoln.ac.uk)

These colony variants show great differences in biofilm formation and motility. (hindawi.com)
A pittii and A. baumannii strains were similar in motility, but A pittii strains had significantly more biofilm production ( P value = 0.018). (biomedcentral.com)
Furthermore Acinetobacter strains were sequenced typed and preliminary characterized for motility and biofilm formation/maintenance. (biomedcentral.com)

We characterized genetic and phenotypic characteris- yeast, other species are found with increasing frequency, tics of isolates from a C. parapsilosis outbreak that including C. parapsilosis (4). (cdc.gov)
In this study, we compare seven STEC isolates associated with those two outbreaks to a diverse but limited collection of ten food, environmental, and animal STEC isolates for genetic and/or phenotypic differences in virulence, stress tolerance, and biofilm formation. (usda.gov)

Although phospholipase B expression has cent of patients who have had Candida isolated from their been well studied in C. albicans (18), the relationship intravenous catheters have underlying fungemia (2), and between C. parapsilosis virulence and phospholipase phe- the case-fatality rate for catheter-related candidemia notype is unclear. (cdc.gov)
The oral Candida strains were isolated from the HIV patients and included species of C. albicans , C. glabrata , C. tropicalis , C. parapsilosis , C. krusei , C. norvegensis , and C. dubliniensis . (biomedcentral.com)
However, all Candida species produced a similar biofilm on acrylic resin, material used in dental prostheses. (biomedcentral.com)
We found that on silicone pads as well as in the Galleria model, biofilm formation and virulence depends on the Candida species. (biomedcentral.com)
Importantly, for C. albicans the pathogenicity of oral Candida isolates was similar to systemic Candida isolates, suggesting that Candida isolates have similar biofilm-forming ability and virulence regardless of the infection site from which it was isolated. (biomedcentral.com)
Among Candida species, C. albicans is the most commonly isolated and responsible for the majority of superficial and systemic infections. (biomedcentral.com)
A major virulence factor of Candida is its ability to adapt to a variety of different habitats and the consequent formation of surface-attached microbial communities known as biofilms [ 5 ]. (biomedcentral.com)
[ 11 ] with Candida albicans being the predominant species isolated in addition to many other candidal species. (medscape.com)

G. mellonella killing rates of HBF isolates were significantly greater than MBF (or LBF) isolates (50% vs. 20%, 2 days from infection). (unicatt.it)
Most significantly, human EAEC causes diarrhoea and death in animals and animal EAEC may form biofilms on human cells. (i-sis.org.uk)
PDT involving a combination of LED and Radachlorin significantly eliminated the biofilm of multidrug-resistant A. baumannii . (ophrp.org)
L. fleischmannii and L. innocua formed significantly less biofilm. (dal.ca)

In man-made water systems microbial biofilms increase the resistance of legionella to disinfection, posing a significant threat to public health. (frontiersin.org)

We compared oral and systemic isolates for the capacity to form biofilm in an in vitro biofilm model and pathogenicity in the Galleria mellonella infection model. (biomedcentral.com)
This study investigated ability of NTHi to form biofilms and its impact on airway epithelia using in vivo and in vitro analyses. (nih.gov)
To further investigate the pathogenesis of NTHi in respiratory infections, we developed a novel in vitro coculture model of NTHi biofilm formation on polarized human airway epithelial cells grown at the air-liquid interface. (nih.gov)
Among 81 CNS included in the current study, 84% showed ability to form biofilm in the experimental in vitro conditions. (edu.pl)
Another objective of this study was to determine the in vitro activity of silver and gold nanoparticles against C. perfringens established biofilm. (lincoln.ac.uk)

Disinfection methods currently used in water systems have been shown to be ineffective against legionella over the long-term, allowing recolonization by the biofilm-protected microorganisms. (frontiersin.org)
Were selected 134 colonies of psychrotrophic microorganisms isolated for sliced ham and 71 of them showed proteolytic activity, 58 lipase activity, 12 showed production of exopolysaccharide. (ufrgs.br)

Octanoic acid effectively clears biofilms (95% eradication at 1X minimum bactericidal concentration, MBC) and infrequently induces S. aureus small colony variants (SCVs) that may cause recurrent mastitis. (ntu.edu.tw)

The systemic strains were isolated from patients with invasive candidiasis and included species of C. albicans , C. glabrata , C. tropicalis, C. parapsilosis, C. lusitaniae , and C. kefyr . (biomedcentral.com)
The quantity of biofilm formed and the virulence in G. mellonella were different for each of the species studied. (biomedcentral.com)
On silicone pads, C. albicans and C. dubliniensis produced more biofilm (1.12 to 6.61 mg) than the other species (0.25 to 3.66 mg). (biomedcentral.com)
While Macrophen and Double Macrophen were the most active substances among polyamino-phenolic ligands, dendrimers were overall twofold more effective than all other compounds with a reduction up to 85 and 73% of legionella and multi-species biofilms, respectively. (frontiersin.org)
Some encapsulated Klebsiella species form glistening mucoid colonies of viscid consistency. (scirp.org)
A. pittii was the most isolated species highlighting the need for proper species identification. (biomedcentral.com)
The isolated strains had limited acute mortality in children, but the occurrence of more multi-drug resistant strains in the future is a distinct possibility, justifying continued research and accurate species identification. (biomedcentral.com)
However, certain Acinetobacter species are frequently isolated increasingly from healthcare facilities and are concomitantly the source of many nosocomial infections [ 1 ]. (biomedcentral.com)

The contribution of the mucoid phenotype to pathogenesis has been demonstrated in several models: in general, the mucoid isolates are more resistant to phagocytosis, less sensitive to serum killing and more virulent in animal studies. (scirp.org)
Seventeen percent of isolates were multi-drug resistant. (biomedcentral.com)
The mature biofilms were more resistant to H 2 O 2 but were all eliminated at 0.2% concentrations. (scielo.org.za)
Listeria monocytogenes is a foodborne pathogen known to colonize surfaces, form antimicrobial-resistant biofilms, and resist environmental stresses such as desiccation, helping it persist in food processing environments. (dal.ca)

Bacterial ability to form biofilms was verified using a crystal violet colorimetric assay and testing cell viability by real-time quantitative PCR and Plate Count assay. (frontiersin.org)
Klebsiella pneumoniae mucoid phenotype and its ability to form biofilm may be important in producing corneal ulceration. (medscape.com)
Their ability to exist as biofilm structures has been alluded to but not extensively investigated. (biomedcentral.com)
The ability of E. meningoseptica isolates to adhere to abiotic surfaces and form biofilm structures may result from the hydrophilic cell surface and multiple adhesins located around the cell. (biomedcentral.com)
Moreover, neofiscalin A, when present at sub-MICs, hampered the ability of both MRSA and VRE strains to form a biofilm. (nih.gov)

Further work has shown the importance of this phage in the P. aeruginosa biofilm lifecycle and virulence. (southampton.ac.uk)
3,6 Biofilm formation by P. aeruginosa is responsible for antimicrobial tolerance and causes major problems for treatment of infections. (rsc.org)
4 found that P. aeruginosa PA14 tended to maximum their contact area with the surface, forming a spontaneous cell alignment between periodic nano-pillars with a post pitch of 2.2, 0.9 and 0.7 μm. (rsc.org)

Bacillus subtilis is a soil-dwelling organism that can be isolated from a wide variety of environments. (nature.com)
The Deep-Sea Microbiology Lab culture collection includes over 400 isolates obtained from deep-sea and shallow-water geothermal environments. (rutgers.edu)

The acid tolerance and heat inactivation profiles of all 17 strains were comparable except for a serotype O157:H7 calf isolate that showed reduced acid tolerance. (usda.gov)

The biofilm formation and pathogenicity in Galleria was similar between oral and systemic isolates. (biomedcentral.com)

Recently, biofilm-forming potential was cited as a reason results suggest that biofilm production plays a role in C. that patients with C. parapsilosis -infected catheters should parapsilosis outbreaks. (cdc.gov)
We evaluated bronchoalveolar lavage fluid from young patients with CF for evidence of NTHi biofilms. (nih.gov)
In bronchoalveolar lavage fluid samples from young, asymptomatic patients with CF, we found morphologic evidence suggestive of NTHi biofilm formation. (nih.gov)
In addition, 10 clinical NTHi isolates from patients with CF formed biofilms on plastic surfaces. (nih.gov)
The M. abscessus isolates recovered from the water were found to be identical to eight isolates from tissue samples from seven of the patients, suggesting that water was the source of the infections. (cdc.gov)
strains were isolated in 70% of the patients. (bvsalud.org)

C. parapsilosis is increasingly responsible for determine genetic variability among isolates from a C. hospital outbreaks, and the hands of healthcare workers parapsilosis outbreak and from our clinical database. (cdc.gov)
Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)

1-5 Dental units have unique characteristics that make them prone to biofilm formation. (cdc.gov)

This organism produces a diverse array of virulence factors, including toxins, adhesins, colonization and biofilm factors. (biomedcentral.com)
This article provides a summary of the epidemic trends, typing methods, virulence factors, biofilm formation mechanisms, and prevention strategies of H. influenzae. (bvsalud.org)

For many decades, biologists followed the standard technique of isolating and purifying an organism to study it. (harvard.edu)
The isolates were identified using standard tests for this organism. (scirp.org)
Example of serious community associated infections due to K. pneumoniae is Friedlander's pneumonia, a severe form of lobar pneumonia with high mortality, and in some countries the organism is the leading cause of pneumonia. (scirp.org)

Biofilm expres- sion by the outbreak clone was greater than that of spo- infection (8,11). (cdc.gov)
Objectives: Biofilm formation (BF) by fungal isolates may dramatically complicate infection. (unicatt.it)
These effects may accelerate the transition from biofilm to chronic infection. (ntu.edu.tw)
Studies of human genetics and HUS have focused mostly on a rare form of the disease that runs in families and is not associated with STEC infection. (cdc.gov)

Outer-membrane protein variation is seen in association with phase variation, but lipopolysaccharide expression is preserved in piliated as well as extensively passaged non-piliated isolates. (microbiologyresearch.org)

Biofilms are notorious for their resistance to environmental stresses, including antimicrobial compounds. (southampton.ac.uk)
Treatment of biofilms with nitric oxide also reduced their resistance to antimicrobial compounds. (southampton.ac.uk)
In addition, there were very few differences among the outbreak strains and the other STEC isolates in their resistance to heat, acid stress, and biofilm formation. (usda.gov)
Among them, 58 isolates showed resistance to ˃100 mg/L of all the metal ions. (nitrkl.ac.in)

Adhesion (15) and biofilm formation (14) may assay was used to quantitate phospholipase. (cdc.gov)
Both isolates displayed a hydrophilic cell surface following the bacterial adherence to xylene assay. (biomedcentral.com)

Twenty-four invasive Acinetobacter isolates were collected from 2009-2013. (biomedcentral.com)
Specifically, A. baumannii , A. pittii , and A. nosocomialis of the Acinetobacter calcoaceticus-baumannii ( Acb ) complex have become the most medically relevant members of the genus as they are most frequently isolated from health care facilities as well as human tissues. (biomedcentral.com)

Clinically obtained C. albicans isolates disks were quantified by dry weight. (cdc.gov)

all HBF and MBF isolates had elevated voriconazole sessile minimum inhibitory concentrations. (unicatt.it)

0.05) in biofilm mode and under different heavy metal concentrations. (nitrkl.ac.in)

We evaluated biofilm efficiency in removing phosphorus, by simulating biofilm-based tertiary wastewater treatment and coupled this process with biodiesel production from the developed biomass. (torvergata.it)

The seventh isolate (serotype O-:H4) was cultured from the same bag of spinach that contained one of the six serotype O157:H7 isolates. (usda.gov)
Biofilm studies showed that only the serotype O-:H4 isolate, but none of the serotype O157:H7 strains, was able to form a biofilm on glass. (usda.gov)
This suggests that strains of E. coli that co-contaminate food products along with serotype O157:H7 strains, may enhance the biofilm forming abilities of the serotype O157:H7 strains and allow for their persistence on food products. (usda.gov)

EAST was found in 17.3 percent of the field strains isolated from internal organs of poultry that died from E. coli infections [13]. (i-sis.org.uk)

Biofilm architecture of isolate CH2B alone as well as in co-culture with Listeria monocytogenes was investigated using flow cells and microscopy. (biomedcentral.com)

Un 40% de esos trabajos han sido publicados en revistas del primer cuartil entre las que destacan New England Journal of Medicine, Lancet, Lancet Infectious Diseases, Clinical Infectious Diseases, Clinical Microbiology Reviews, Antimicrobial Agents and Chemotherapy, Journal Clinical Microbiology, Journal Antimicrobial Chemotherapy, Mayo Clinic Proceedings y Malaria Journal. (unav.edu)

Additionally, exploring the regulatory differences between CA-MRSA and other MRSA strains may aid our understanding of the increase in virulence observed amongst community-associated isolates. (biomedcentral.com)
Biofilm production for 182 MRSA isolates obtained from clinical culture sites (2004 to 2013) was quantified. (uri.edu)

Biofilm formation in anaerobic organisms is under-researched. (lincoln.ac.uk)

This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus . (hindawi.com)
V. alginolyticus ZJ-51, isolated from diseased grouper, has shown colony phase variation between opaque/rugose (Op, opaque variant) and translucent/smooth (Tr, translucent variant). (hindawi.com)
Based on this, we wanted to identify this protein in V. alginolyticus ZJ-51 and study whether it has a role in biofilm formation and stress response in the two colony phase variants. (hindawi.com)
Two V. alginolyticus strains (V590 and V595) isolated from hake fish as well as the type strains of V. alginolyticus LMG 4409 and V. parahaemolyticus LMG 2850 were used. (scielo.org.za)

My research group aims to understand how biofilms develop and disperse and how they respond to environmental stresses including therapeutic compounds. (southampton.ac.uk)

Transition from a slow-growing, dry agar-pitting (DAP) to a faster-growing, smooth non-agar-pitting (SNP) form appears to occur predictably and may reflect competition between two populations growing at different rates. (microbiologyresearch.org)
6 Biofilms occur in dental unit waterlines due to the long, small-diameter tubing and low flow rates used in dentistry and the frequent periods of stagnation. (cdc.gov)

NTHi formed biofilms on the apical surface of cultured airway epithelia. (nih.gov)

Anatomy2

Organisms14

Diseases6

Chemicals and Drugs25

Analytical, Diagnostic and Therapeutic Techniques and Equipment19

Phenomena and Processes26

Disciplines and Occupations4

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture3

Information Science3

Health Care6

Bacteria26

Inhibition11

Strong biofilm6

Pseudomonas8

Antibiotic8

Assays4

Bacterial isolates3

Staphylococcus5

Bacterium11

Preventing biofilm formation1

Communities called biofilms1

Persistent2

Outbreak isolates2

Genes9

Formation of biofilms1

Extracellular2

Strain4

Susceptibility8

Anti-biofilm3

Control biofilm2

Motility3

Phenotypic2

Candida8

Significantly4

Microbial1

Vitro5

Microorganisms2

Aureus1

Species8

Resistant4

Ability5

Aeruginosa3

Environments2

Tolerance1

Pathogenicity1

Patients6

Increasingly2

Characteristics1

Virulence factors2

Organism3

Infection4

Extensively1

Resistance4

Assay2

Acinetobacter2

Clinically1

Inhibitory1

0.051

Biomass1

Serotype3

Strains isolated1

Listeria1

Microbiology1

MRSA2

Anaerobic1

Alginolyticus4

Environmental stresses1

Occur2

Apical1