These specific ethanol tolerance related traits/proteins/genes can be used by researchers for developing more ethanol tolerant biocatalysts for biomass to biofuel conversion. (usda.gov)
We have performed transcriptional and proteomic studies of yeasts present in the pulmonary granulomas of PCM aiming to identify relevant genes and proteins that act under stressing conditions. (bvsalud.org)
Furthermore, transcriptomics and proteomics analyzes indicated a low fungal activity within the granuloma, as demonstrated by the decreased expression of genes and proteins related to energy metabolism and cell cycle. (bvsalud.org)
Interestingly, while genes which commonly code for cellusomes, protein complexes which breakdown crystalline cellulose, are present the organism is unable to grow solely on cellulose substrates (7). (thecoffeeparlor.com)
Recombinant1
The core expertise of the researchers and students covers genomics/metagenomics, next generation sequencing, bioinformatics, MS-based proteomics, recombinant protein expression, and bioconversion of marine biomasses (industrial side streams). (uit.no)
Compounds1
In the project MicroMBT we explored the potential of microbial biofilm communities to convert marine protein hydrolysates into high value compounds, and in BMFishFeed we currently aim to use microbial consortium to bring industrial side stream materials back into circulation, as feed additives for aquaculture. (uit.no)
Strain3
A novel strain of Lactobacillus buchneri NRRL B-30929 was isolated as a contaminant of a fuel ethanol production facility. (usda.gov)
With the above mentioned unique characteristics, plus the genome sequence data, this strain can be used by researchers for developing new biocatalysts that convert biomass derived sugars into ethanol and other value-added products. (usda.gov)
A second wild-type isolate (strain LS3 (PAR-4)) with characteristics similar to CBS 8244 T was selected from wood hydrolysates in Siberia, and additional strains were later isolated from chopped maize silage or humus-rich soil. (biomedcentral.com)
Convert1
Thus, robust and improved biocatalysts are needed to convert lignocellulosic biomass hydrolysates into fuels and valuable chemicals. (usda.gov)
Yeast2
Protein components of different pathways for carbon and nitrogen source utilization were identified, which so far has remained unexplored in yeast, offering clues for further biotechnological developments. (biomedcentral.com)
Primig, M.: Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development. (tu-dresden.de)
Production3
Conventional Saccharomyces for starch to ethanol fermentation are no longer applicable for lignocellulose-based ethanol production since a mixture of sugars instead of glucose alone is present in biomass hydrolysates. (usda.gov)
Xylitol production using hemicellulosic hydrolysates. (tu-dresden.de)
From the end of January to mid-February our elective modules Optimization Processes in Protein Production and Natural Product Biosynthesis take place at our institute. (uni-duesseldorf.de)
High1
The identification of ethanol stress related proteins can facilitate understanding of the molecular mechanisms as how the microbe works to tolerate high ethanol concentrations. (usda.gov)
Process2
George, and S.M. Williams and Wilkins, Baltimore, MD. Currently, there is a resurgence of interest in Clostridium acetobutylicum, the biocatalyst of the historical Weizmann process, to produce n-butanol for use both as a bulk chemical and as a renewable alternative transportation fuel. (thecoffeeparlor.com)
Xyl10B is a candidate biocatalyst in the bleaching process of pulp and the paper industries because of its inactivity on carboxymethyl cellulose. (springeropen.com)
Environmental1
We have established a large collection of environmental samples from the Arctic (sediments, biota & sea water) as well as culture collection of more than a thousand bacterial isolates. (uit.no)
Cells1
To solve this dilemma, cells must use their own proteins as sources of amino acids in response to the nutritional downshift. (go.jp)
Enzymes6
Different specific and non-specific enzymes are used to obtain hydrolysates (trypsin, pepsin, pancreatin and alcalase). (intechopen.com)
2012 ). When using enzymes for digesting food proteins, an enzyme specific peptide composition, the so-called peptide fingerprint is formed. (springeropen.com)
For food protein hydrolysates, the substrate and enzymes are mixed in huge, tempered tanks, and require continuous stirring, as well as a constant temperature and pH level for several hours. (springeropen.com)
To reduce energy and enzyme costs and produce continuously defined hydrolysates, the enzymes need to be stabilized. (springeropen.com)
However, enzymes isolated from nature must be adapted to industrial requirements, such as the ability to tolerate high temperatures. (bvsalud.org)
Microorganisms are a valuable source of enzymes for both industrial and medical uses because of their rapid growth rate and simplicity of manipulation, especially with the advent of recombinant DNA technology and protein engineering [ 1 ]. (springeropen.com)
Substrate2
In an activated form, they hydrolyze the protein substrate at specific sites and the released peptides can be absorbed. (springeropen.com)
The toxic nature of the lignocellulosic hydrolysate severely impedes its efficient substrate utilization and bioethanol fermentation. (springeropen.com)
Whey protein1
The most studied substrates used to produce bioactive peptides are milk proteins in the form of co-products from dairy industries: caseins, cheese whey, buttermilk, whey protein concentrates and isolates or even pure single proteins that can be obtained at a reasonable price on an industrial scale (e.g. β-lactoglobulin, β-Lg). (intechopen.com)
Peptide3
Separation technologies, which discriminate small differences in charge, size and hydrophobicity, can be employed to fractionate protein hydrolysates and obtain peptide fractions with higher functionality or higher nutritional value in a more purified form. (intechopen.com)
The hydrolysates composition underlies process-related fluctuations and therefore the obtained peptide fingerprint and bioactive properties may vary. (springeropen.com)
To overcome this obstacle and enable the production of specific hydrolysates with selected peptides, a ceramic capillary system was developed and characterized for the continuous production of a consistent peptide composition. (springeropen.com)
Microbial1
Research thrust 1 is to understand the principles of modular design in biological systems and develop the transformative MODCELL (Modular Cell) technology to engineer modular chassis cells for rapid development of novel microbial biocatalysts for industrial biocatalysis. (utk.edu)
Hydrolysis5
Nowadays the most common way to obtain bioactive peptides is by enzymatic hydrolysis of protein solutions. (intechopen.com)
Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. (springeropen.com)
In industrial processes, protein hydrolysis is often carried out in batch processes and is mostly used in fermentation and enzyme based processes, like brewing, cheese manufacturing, meat tenderization or baking (Godfrey and Reichelt 1982 ). (springeropen.com)
Hydrolysis is conducted by an enzymatic or acidic breakdown of proteins (Tsugita and Scheffler 1982 ). (springeropen.com)
Although Z. mobilis demonstrates advantages in ethanol fermentation, the inhibitors derived from biomass deconstruction and hydrolysis still have detrimental effects on Z. mobilis , especially when high biomass solid loading is used during pretreatment and enzymatic hydrolysis steps to generate high concentration of sugars besides the potential additive or synergistic inhibitions of various inhibitors in the hydrolysate (Franden et al. (springeropen.com)
Composition1
The catalytic activity of some of them is quite specific and the composition of the hydrolysate is predictable when substrates are quite pure [ 1 ]. (intechopen.com)
Zymomonas mobilis is a natural ethanologenic bacterium with many desirable industrial characteristics, but it can also be severely affected by lignocellulosic hydrolysate inhibitors. (springeropen.com)
To make the flavin-containing monooxygenase (FMO) Methylophaga aminisulfidivorans trimethylamine monooxygenase (mFMO) more suitable for industrial application, we engineered it using the Protein Repair One-Stop Shop (PROSS) algorithm. (bvsalud.org)
Moreover, unlike the native enzyme, the best thermostable variant efficiently oxidized TMA in a salmon protein hydrolysate at industrial temperatures. (bvsalud.org)
Form1
Keratin is the major structural fibrous protein belonging to the large family of structural proteins to form hair, wool, feathers, nails, and horns of many kinds of animals and has a high concentration of cysteine, 7-20% of the total amino acid residues, that form inter- and intramolecular disulfide bonds. (intechopen.com)
Analysis2
In chromatographic analysis the hydrolysate is separated and the peptides show a highly specific and reproducible pattern. (springeropen.com)
Keratins are everywhere, from being the major components of household dust to common contaminants of laboratory protein analysis. (intechopen.com)
Results1
Results showed that the isolated strain belonged to the genus Halobacillus , and it was designated as Halobacillus sp. (springeropen.com)
Important1
Food proteins are important macronutrients providing the human body with essential amino acids. (springeropen.com)
Reduce2
Bacterial trimethylamine monooxygenases can oxidize TMA into the odorless trimethylamine N-oxide (TMAO) and have been shown to reduce TMA levels in a salmon protein hydrolysate. (bvsalud.org)
Finally, mFMO_20 significantly outperformed native mFMO in its ability to reduce TMA levels in a salmon protein hydrolysate at industrially relevant temperatures. (bvsalud.org)