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AeromonasAeromonas hydrophilaAeromonas salmonicidaGram-Negative Bacterial InfectionsAeromonas caviaeDNA, BacterialMicrobial Sensitivity TestsWater MicrobiologySequence Analysis, DNAMolecular Sequence DataAnti-Bacterial AgentsBacterial Typing TechniquesPhylogenyFish DiseasesBacterial ProteinsFurunculosisVirulenceRNA, Ribosomal, 16SPolymerase Chain ReactionElectrophoresis, Gel, Pulsed-FieldFecesDrug Resistance, BacterialDiarrheaHemolysin ProteinsVibrionaceaeDNA FingerprintingSerotypingCytotoxinsGenes, BacterialDrug Resistance, Microbialbeta-LactamasesSpecies SpecificityBase SequenceEnterobacteriaceaePolymorphism, Restriction Fragment LengthCulture MediaGenotypeBacterial InfectionsEnterotoxinsDrug Resistance, Multiple, BacterialBacterial ToxinsVirulence FactorsPhenotypeRandom Amplified Polymorphic DNA TechniqueFishesGenetic VariationCluster AnalysisFood MicrobiologyAquacultureEscherichia coliMolecular EpidemiologyTroutFresh WaterNucleic Acid HybridizationAmino Acid SequenceSalmonidaeDNA GyraseMultilocus Sequence TypingPlasmidsEnvironmental MicrobiologyGastroenteritisPore Forming Cytotoxic ProteinsBacteriaDrainage, SanitaryBacteriological Techniques
PseudomonasBacterial IsolatesResistanceVibrioSusceptibleGenesEscherichiaSusceptibilityAmpicillinHydrophila isolatesCharacterization of AeromonasESBLSalmonicidaPathogensMicroorganismsVirulenceMotileSpecies isolatedClinicalStreptococcusFishesPathogenicResistantColiStrains isolatedVirulentCaviae2020MolecularBiochemical testsStrainBacillusStanierBacteria isolatedIdentificationSobriaGeneticSalmonellaGenomeGenusStoolPathogenicityPredominantAssaysGenomicAgarListeriaPresenceAquaticBurkholderia
Pseudomonas5
  • Six isolates were identified as Pseudomonas sp. (scialert.net)
  • The isolates were characterized and identified as Bacillus licheniformis, Aeromonas hydrophila , Bacillus circulans, Proteus morganii , Pseudomonas cepacia , Bacillus alvei and Pseudomonas chlororaphis . (scialert.net)
  • It was found that as the pH of the growth medium increased from 3-9, the rate of growth of most isolates also increased except for Pseudomonas cepacia, which had optimum growth at neutral pH 7. (scialert.net)
  • Additional bacterial organisms that may be isolated in cases of necrotizing fasciitis include Escherichia coli , Klebsiella , Pseudomonas , Proteus , Vibrio , Bacteroides , Peptostreptococcus , Clostridium , and Aeromonas hydrophila , among others. (emedicinehealth.com)
  • If history reflects exposure to sewage-contaminated water, gram-negative coverage should be instituted for organisms such Pseudomonas and Aeromonas . (medscape.com)
Bacterial Isolates2
  • Comparison of biochemical and molecular methods for the identification of bacterial isolates associated with failed loggerhead sea turtle eggs. (scientificwebjournals.com)
  • Comparison of the API 20E and BBL Crystal E/NF identification systems for differentiating bacterial isolates from apparently healthy reared sea bass (Dicentrarchus labrax). (scientificwebjournals.com)
Resistance18
  • Although, almost all previous studies and efforts to control the dissemination of these genes have been based on isolates from clinical samples, antibiotic resistance genes can also occur in nonpathogenic bacteria, which can then be transferred via lateral gene transfer (6). (scielo.br)
  • Herbal and antibiotic resistance of Aeromonas bacteria isolated from cultured fish in Egypt and Malaysia. (upm.edu.my)
  • Results revealed high resistance of the 6 tested Aeromonas isolates against most of the screened antibiotics except Ciprofloxacin and Gentamicin. (upm.edu.my)
  • Regarding herbal sensitivity, only Origanum vulgare showed an efficacy and inhibition zone against all isolates except A. hydrophila isolated from cultured tilapia in Egypt which exhibited resistance to that herb. (upm.edu.my)
  • Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp. (jidc.org)
  • Data of stool specimens yielding growth of non-O1 and non-O139 V. cholerae isolated at a national referral laboratory from 1999 to 2012 were retrospectively analysed and evaluated for resistance to ampicillin, tetracycline, chloramphenicol, co-trimoxazole and ofloxacin. (who.int)
  • In all the samples, 50 E. coli strains were isolated and then assigned to serotyping, antimicrobial susceptibility test, detection of antimicrobial resistance genes and the Class 1 integrons, and multilocus sequence typing (MLST). (hindawi.com)
  • The antibiotic susceptibility profile of the seven isolates was determined and it was discovered that 65% of the microorganisms isolated were resistance to the antibiotic used while 35% were sensitive. (scialert.net)
  • Therefore, the aim of this study was to evaluate the level of antibiotic resistance in isolates carrying bla VIM, bla KPC, bla NDM, bla IMP, bla SHV, bla TEM, bla CTX-M and class I and II integrons in Aeromonas hydrophila. (clin-lab-publications.com)
  • Conclusions: The results of the study of antibiotic resistance, beta-lactamase, and carbapenemase genes showed high resistance in Aeromonas hydrophila, which raises concerns with regard to controlling infection in medical centers. (clin-lab-publications.com)
  • High levels of resistance were however expressed by all isolates to penicillin, oxacillin and ampicillin. (e-fas.org)
  • Five P. multocida isolates (3.6%), all obtained from NSW in 2019, exhibited dual resistance to macrolides and tetracycline, and a further two Queensland isolates from 2019 (1.4%) exhibited a multidrug-resistant phenotype to ampicillin/penicillin, tetracycline, and tilmicosin. (edu.au)
  • Illumina whole genome sequencing identified the genes msr(E) and mph(E)encoding macrolide resistance, tet(R)-tet(H) or tet(Y) encoding tetracycline resistance, and blaROB-1 encoding ampicillin/penicillin resistance in all isolates exhibiting a corresponding resistant phenotype. (edu.au)
  • To evaluate disease resistance, 15 fish in each treatment were infected in each treatment by Aeromonas hydrophila . (ij-aquaticbiology.com)
  • Phenotypic and genotypic virulence factors and antimicrobial resistance of motile Aeromonas spp. (scientificwebjournals.com)
  • Study of prevalence and antibiotic resistance in Aeromonas species isolated from minced meat and chicken samples in Iran. (scientificwebjournals.com)
  • Also, 12.5% isolates showed complete resistance while another 12.5% were susceptible. (researchwap.com)
  • Furthermore, 62.5% of the isolates were susceptible to the ethanoic extracts of G. officinale, while in 25% antimicrobial activity was intermediate and in 12.5%, there was complete resistance.Moringaolifera ethanoic extract had low antimicrobial effects in 50% of the isolates, intermediate in 25%, 12.5% were completely resistant, while 12.5% were susceptible. (researchwap.com)
Vibrio6
  • RÉSUMÉ Au cours des dix dernières années, l'importance des souches de Vibrio cholerae non-O1 et non-O139 a été mise en avant à l'échelle mondiale. (who.int)
  • This study was conducted to isolate bacterial micro flora from digestive tract of healthy juvenile white shrimp, Litopenaeus vannamei based on antagonistic activity against shrimp pathogen, Vibrio parahaemolyticus . (scialert.net)
  • In addition three Vibrio species including V. parahaemolyticus, Vibrio alginolyticus and Vibrio cholerae were isolated from moribund shrimp and used as pathogen in this study. (scialert.net)
  • isolates were tested for antibacterial activity against three Vibrio species using cross streak assay. (scialert.net)
  • The most important losses have been at the larval and juvenile stages, and vibriosis has long been the most important bacterial disease in cod, with Listonella ( Vibrio) anguillarum dominant among pathogenic isolates. (int-res.com)
  • Notably, some blood culture isolates were seen in outborn neonates in the first week of life but not in inborns: Salmonella, Aeromonas, and Vibrio spp. (ox.ac.uk)
Susceptible5
  • Eighty-one percent of isolates were susceptible to penicillin and/or gentamicin and 84% to ampicillin and/or gentamicin. (ox.ac.uk)
  • All isolates tested were susceptible to at-least ten (10) of the fourteen antibiotics evaluated. (e-fas.org)
  • The exception was the tilmicosin-resistant, tulathromycin/gamithromycin-susceptible phenotype identified in two Queensland isolates, the genetic basis of which could not be determined. (edu.au)
  • The majority of the isolates were resistant to amoxacillin and ampicillin but susceptible to ceftriaxone. (bvsalud.org)
  • These agents are active against Staphylococcus aureus (including methicillin-susceptible and methicillin-resistant S aureus [MSSA, MRSA] isolates), Streptococcus pyogenes , Streptococcus agalactiae , and Streptococcus anginosus group (includes Streptococcus anginosus , Streptococcus intermedius , and Streptococcus constellatus ), among others. (medscape.com)
Genes11
  • Presence and phenotypic expression of blaTEM, blaSHV, and blaCTX-M ESBL-encoding genes were investigated in environmental water isolates of Aeromonas hydrophila and Aeromonas jandaei. (scielo.br)
  • Presence of blaSHV and blaCTX-M genes was not observed, and blaTEM gene was verified in 91% of the isolates. (scielo.br)
  • Presence of bla SHV and bla CTX-M genes was not observed, and bla TEM gene was verified in 91% of the isolates. (scielo.br)
  • The aim of this study was to investigate the presence and phenotypic expression of bla TEM , bla SHV, and bla CTX-M ESBL- encoding genes in 87 environmental water isolates of Aeromonas hydrophila (n=41) and Aeromonas jandaei (n=46). (scielo.br)
  • Prevalence of enterotoxin genes in Aeromonas spp. (ox.ac.uk)
  • We studied the distribution of the alt, ast, and act enterotoxin genes in 115 of 125 aeromonads isolated from 1, 735 children with diarrhea, in all 27 aeromonads isolated from 830 control children (P = 7 x 10(-4) for comparison of rates of isolation of aeromonads from cases versus those from controls), and in 120 randomly selected aeromonads from different components of surface water in Bangladesh. (ox.ac.uk)
  • Importantly, the number of isolates positive for both the alt and ast genes was significantly higher for diarrheal children than for control children and the environment. (ox.ac.uk)
  • Analysis of clinical data from 11 of these patients suggested that isolates positive for both the alt and ast genes were associated with watery diarrhea but that isolates positive only for the alt gene were associated with loose stools. (ox.ac.uk)
  • Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli . (vetmedmosul.com)
  • Array analysis revealed 18 isolates with ESBL genes and one isolate with solely non-ESBL beta-lactamase genes. (afpm.org.my)
  • Detection of aerolysin, hemolysin genes and antimicrobial susceptibility of Aeromonas hydrophila isolated from retail foods and human stool samples. (scientificwebjournals.com)
Escherichia2
  • In this study, the prevalence, phenotypes, and clonal relationships of Escherichia coli ( E. coli ) strains isolated from minks were investigated. (hindawi.com)
  • The isolates were identified as Klebsiella pneumoniae (n=9), Escherichia coli (n=6), Enterobacter cloacae complex (n=5) and Enterobacter spp. (afpm.org.my)
Susceptibility1
  • Here, the susceptibility of Mannheimia haemolytica and Pasteurella multicoda isolates obtained from BRD clinical cases (deep lung swabs at post-mortem) among feedlots in four Australian states (2014-2019) was determined for 19 antimicrobial agents. (edu.au)
Ampicillin1
  • In isolation, 5 different selective media were used: DNase Agar with Toluidine blue and Ampicillin (DNTA), Aeromonas Agar (AA), Rimler Shotts Agar (RSA), mA Agar and Aeromonas Mediums Base (AMB). (tubitak.gov.tr)
Hydrophila isolates2
  • The PCR technique is modern method which regarded as a reliable tool to detect virulent gene of the A. hydrophila isolates. (vetmedmosul.com)
  • The higher percentage of the A. hydrophila isolates found in the raw cow milk was 40%, and the A . hydrophila isolates found in the raw buffalo milk was 26.7%, while, the A. hydrophila isolates did not find in the goat milk. (vetmedmosul.com)
Characterization of Aeromonas3
ESBL4
  • All isolates were screened for ESBL production by a double-disc synergy test using clavulanic acid (amoxicillin- clavulanate disk , 20/10 µg) and cloxacillin (200 µg/ml-containing Mueller-Hinton agar plates) as ESBL and AmpC inhibitors, respectively, and ceftazidime, cefpodoxime and cefotaxime-containing disks as ESBL substrates (9). (scielo.br)
  • The need for ESBL detection is under challenge on the supposition that it is possible to set breakpoints for injectable cephalosporins and aztreonam that accurately discriminate which ESBL-producing isolates can and cannot be reliably treated with these drugs. (asm.org)
  • Among the 100 isolates, 60 isolates produced broad-spectrum beta-lactamase (ESBL) and 50 isolates produced carbapenemase. (clin-lab-publications.com)
  • Samples were collected from fresh culinary herbs imported from Southeast Asia in which ESBL-suspected isolates were obtained by selective culturing. (afpm.org.my)
Salmonicida2
  • Vaccination of cod against pathogens such as L. anguillarum and Aeromonas salmonicida clearly demonstrates that the cod immune system possesses an effective memory and appropriate mechanisms sufficient for protection, at least against some diseases. (int-res.com)
  • Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. (shengsci.com)
Pathogens5
  • isolates number 5, 7, 15 and 30 against three pathogens. (scialert.net)
  • Bacterial pathogens were isolated from some Nigerian packaged fruit juices. (scialert.net)
  • Coaggregation by isolate CH2B appeared to be strongest with foodborne pathogens like Enterococcus, Staphylococcus and Listeria spp. (biomedcentral.com)
  • Therefore, this study is aimed at isolating and characterizing microbial pathogens of post-harvest rot in yam. (researchwap.com)
  • Results obtained, showed the presence of known bacterial pathogens of yam rot such as Streptococcus sp, Staphylococcus aureus, Aeromonas sp, Enterococcus sp and Micrococcus sp. (researchwap.com)
Microorganisms1
Virulence3
Motile3
Species isolated1
  • nov., a novel species isolated from aquatic samples. (dsmz.de)
Clinical3
Streptococcus1
  • 25%), Coagulase negative staphylococci (2/8;25%), Klebsiella pneumoniae (2/8;25%) and Streptococcus pyogenes 2/8(25%) were isolated. (bvsalud.org)
Fishes1
Pathogenic1
  • Thus, the sensitivity of certain commercial antibiotics and common herbs was evaluated against pathogenic A. hydrophila, A. sobria and A. caviae, isolated from Malaysian and Egyptian cultured fish, mainly tilapia. (upm.edu.my)
Resistant3
  • All isolates tested were multidrug resistant. (afpm.org.my)
  • The antimicrobial effect of M. olifera methanoic extracts was low in 12.5% isolates while 87.5% were completely resistant. (researchwap.com)
  • Many multidrug resistant bacterial species were isolated. (bvsalud.org)
Coli3
  • This may be unnecessary for community isolates, which at this time appear to be predominantly CTX-M-producing E. coli . (asm.org)
  • Compared with 340 human E. coli STs reported in China, the ST10 clonal complex, known as the largest human clonal complex, was also found in the 50 mink E. coli isolates. (hindawi.com)
  • Enterobacter E. coli and Aeromonas sp. (ejpmr.com)
Strains isolated1
Virulent2
  • Identification and characterization of differentially expressed channel catfish IgM transcripts after vaccination with antigens of virulent aeromonas hydrophila. (usda.gov)
  • Subashkumar R, Thayumanavan T, Lakshmanaperumalsamy P.RAPD and ERIC-PCR typing of virulent Aeromonas hydrophila isolated from children with acute diarrhoea. (vetmedmosul.com)
Caviae2
  • Pulsed-field gel electrophoresis patterns and similarity dendrogram of genomic DNA from Aeromonas hydrophila and A. caviae isolates from diarrheic stool (S) and groundwater (W). The number refers to the isolate number. (cdc.gov)
  • The MIC ranged from 25-55% for the Egyptian isolates (25% for A. caviae and 55% for A. sobria) and 24-30% for the Malaysian isolates (24% for A. hydrophila, 25% for A. caviae and 30% for A. sobria). (upm.edu.my)
20202
  • B. pseudomallei isolates from both patients and the environmental samples were all genetically similar and were distinct from previous known isolates, indicating bacteria from the environment was the likely source of infection for both patients and has been present in the area since at least 2020. (cdc.gov)
  • Fernández-BravoA,JoséFiguerasM.AnUpdateontheGenus Aeromonas: Taxonomy,Epidemiology,andPathogenicity.microorganismsJ.2020;8(129):2-39.https://doi:10.3390 microorganisms8010129. (vetmedmosul.com)
Molecular2
  • Molecular characterisation, genotyping and survival of Aeromonas hydrophila isolated from milk, dairy products and humans in Egypt. (vetmedmosul.com)
  • In recent years, phenotypic identification kits have been reported to give incorrect results in identifying Aeromonas species, whereas molecular identification is quite reliable. (scientificwebjournals.com)
Biochemical tests2
  • The collected isolates were identified by standard biochemical tests. (clin-lab-publications.com)
  • The isolates were identified by their morphological characteristics, conventional biochemical tests and Analytical Profile Index test kits. (e-fas.org)
Strain2
  • The ability of Elizabethkingia meningoseptica isolate CH2B from freshwater tilapia ( Oreochromis mossambicus ) and E. meningoseptica strain NCTC 10016 T to adhere to abiotic surfaces was investigated using microtiter plate adherence assays following exposure to varying physico-chemical challenges. (biomedcentral.com)
  • In this study, we explored both hypotheses elucidating the pan-genome of Vibrionaceae isolates after a perturbation event in the endangered oasis of Cuatro Ciénegas Basin (CCB), Mexico, and looking for signals of adaptation to the environments in their genomes.Results We obtained 42 genomes of Vibrionaceae distributed in six lineages, two of them did not showed any close reference strain in databases. (researchsquare.com)
Bacillus2
  • It was also noted that as concentration of benzoic acid increased from 250-1000 mg/l Bacillus licheniformis decreased from 1.330-0.167 nm, Aeromonas hydrophila decreased from 1.208-0.164 nm Bacillus circulans decreased from 1.158-0.299 nm, Proteus morganii decreased from 1.377-0.141 nm etc. (scialert.net)
  • Background: Aeromonas hydrophila is an opportunistic gram-negative bacillus that causes diseases such as gastroenteritis, muscle infections, soft tissue, sepsis, and skin diseases in humans. (clin-lab-publications.com)
Stanier2
  • To download a certificate of analysis for Aeromonas hydrophila (Chester) Stanier ( 43414 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of Aeromonas hydrophila (Chester) Stanier ( 43414 ) is not currently available online. (atcc.org)
Bacteria isolated1
  • A total of 288 fish samples from 40 fish farms (ponds, cages, and tanks) and 8 wild water sites were aseptically collected and bacteria isolated from the head kidney, liver, brain and spleen. (e-fas.org)
Identification2
  • The identification of Aeromonas species was performed as previously described (1). (scielo.br)
  • Identification of the isolates was performed according to Bergey's Manual of Determinative Bacteriology (1984) and 9 isolates were identified as A. hydrophila. (tubitak.gov.tr)
Sobria1
  • Aeromonas hydrophila (43.8%), Aeromonas sobria (20.8%), Edwardsiella tarda (8.3%), Flavobacterium spp. (e-fas.org)
Genetic1
  • Random-amplified polymorphic DNA (RAPD) typing identified a high degree of genetic homogeneity among the M. haemolytica isolates, whereas P. multocida isolates were more heterogeneous. (edu.au)
Salmonella1
  • In this study, a Salmonella Typhimurium lytic bacteriophage, Φ st1, which was isolated from chicken faecal material, was evaluated as a candidate for biocontrol of Salmonella in chickens. (afpm.org.my)
Genome3
  • Whole genome sequencing and annotation of seven strains of aeromonas veronii isolated from channel catfish. (usda.gov)
  • The isolates from all patients were submitted to CDC for whole genome sequencing which indicated genomic sequences were clonal to one another and were potentially of South Asian origin. (cdc.gov)
  • Whole-Genome Sequence of Aeromonas spp. (tamu.edu)
Genus1
  • Members of the genus Aeromonas have been associated with a wide range of illnesses in humans, including gastrointestinal disorders and systemic infections in both immunocompromised and healthy hosts (4). (scielo.br)
Stool2
Pathogenicity1
  • Alberto C,Javier Y, Alejandro T,Maria SA,Carmen H, Jose A,German N.A Major secreted elastase is essential for pathogenicity of Aeromonas hydrophila .Infect Immuol.2000;68(6):3233-3241. (vetmedmosul.com)
Predominant1
  • MLST analysis showed that 32 sequence types (STs) were identified, while ST46 was the predominant genotype among all isolates. (hindawi.com)
Assays1
  • isolates, while the role of cell-free supernatants, from diverse bacteria, in inducing enhanced adherence was investigated using microtitre plate assays. (biomedcentral.com)
Genomic1
  • Genomic analysis of the four patient isolates grouped closely with strains from South Asia. (cdc.gov)
Agar2
  • A suspension of freshly cultured isolates was prepared and spread over the Muller's Hinton agar plates to study their sensitivity to both antibiotics and herbs. (upm.edu.my)
  • Isolates were tested for antimicrobial activities by measuring zones of inhibition on Mueller-Hinton agar. (researchwap.com)
Listeria2
Presence4
  • Several studies have shown the presence of Aeromonas spp. (scielo.br)
  • and isolates positive only for the presence of the act gene were not found in any of the three sources. (ox.ac.uk)
  • A total of 43 samples (4 sea water, 5 water, 4 river water, 10 oyster and 10 fish samples) from Izmir were examined for the presence of Aeromonas hydrophila. (tubitak.gov.tr)
  • Partial inhibition of coaggregation was observed when isolate CH2B was treated with heat or protease exposure, suggesting the presence of heat-sensitive adhesins, although sugar treatment resulted in increased coaggregation and may be associated with a lactose-associated lectin or capsule-mediated attachment. (biomedcentral.com)
Aquatic1
  • Aeromonas species obtained from different farmed aquatic species in India and Taiwan show high phenotypic relatedness despite species diversity, BMC Research Notes, 14, 313. (scientificwebjournals.com)
Burkholderia1
  • MALDI-TOF, 16s, VITEK-2) may misidentify B. pseudomallei as another bacterium, such as occurred with the case in TX, where the isolate was initially misidentified as Burkholderia thailandensis by MALDI-TOF. (cdc.gov)