Isogenic laboratory mouse strains. Medical search. Frequent questions

The interesting activities of a subset of 4-hydoxy-2-pyridones provided the impetus for synthesis of additional chemotypes with this core ( Figure 1 ) and their evaluation for effectiveness against additional pathogenic strains including Ng and N. meningitidis (Nm ). (elifesciences.org)
Pathogenic strains then produce two toxins (TcdA and TcdB), which are considered as the major virulence factors, that lead to the disruption of the actin cytoskeleton of intestinal epithelium cells (6,7), therefore, conferring the CDI symptoms (diarrhoea, epithelial apoptosis and ulceration). (studyres.com)
In an adaptive immunity OPC rechallenge model using a derivate of the pathogenic C. albicans strain SC5314, CD4 + Th17 cells protect from mucosal Candida infection but can be compensated by other IL-17-producing cells in CD4-deficient hosts ( 15 , 16 ). (frontiersin.org)
In addition, Uro-pathogenic strains are usually resistant to serum bactericidal activity [ 8 ]. (biomedcentral.com)

Human retinal-pigment epithelial cells were infected with Pg, and its isogenic mutant strains and genes were analyzed by quantitative polymerase chain reaction (qPCR). (dentistrytoday.com)
Using in vitro biofilm, adherence and invasion assays with immortalized human vaginal epithelial VK2 cells, and a mouse model of GBS vaginal colonization, we tested the contribution of CAMP factor using GBS strain COH1 and its isogenic CAMP-deficient mutant (Δcfb). (bvsalud.org)
To address the question of specificity for the allele encoding the Na V 1.4-R669H variant as a cause of HypoPP and to produce a model system in which to characterize functional defects of the mutant channel and susceptibility to paralysis, we generated knockin mice carrying the ortholog of the gene encoding the Na V 1.4-R669H variant (referred to herein as R669H mice). (jci.org)
A comparison of the proteins secreted by UCN34 wild type and its isogenic ΔessC mutant revealed six T7SSb effector proteins, including the expected WXG effector EsxA and three LXG-containing proteins. (pasteur.fr)

Additional alginate loci were located within the P. aeruginosa PAO1 genome utilizing isogenic mucoid mutants coupled with PAO1-derived cosmid complementation. (marshall.edu)
C57BL/6J is the most widely used inbred strain and the first to have its genome sequenced. (ncard.org.au)

Adaptive responses of evolved Saccharomyces cerevisiae strains tolerant to acidic pH, acetic acid, and supraoptimal temperature. (uaem.mx)

Congenic BALB/c CL4 strain expressing C57Bl/6 Thy 1.1 allele. (ncard.org.au)
JAX stock No# 002216 or C57BL/6J isogenic Rag1 knock-out strain is also available as Stock No. 034159 . (ncard.org.au)
In this study we have increased the density of microsatellite markers 2- to 5-fold in each of several major RI sets that share C57BL/6 as a parental strain (AXB, BXA, BXD, BXH, and CXB). (nervenet.org)

Isogenic organisms have identical, or near identical genotypes. (wikipedia.org)
One of the key strengths of using inbred strains as a model is that strains are readily available for whatever study one is performing and that there are resources such as the Jackson Laboratory, and FlyBase, where one can look up strains with specific phenotypes or genotypes from among inbred lines, recombinant lines, and coisogenic strains. (wikipedia.org)
Genotypes of more than 100 RI strains were extensively error checked and regenotyped. (nervenet.org)
A third advantage of RI strains is that genotypes generated by different groups using a variety of methods can be pooled to generate high-density linkage maps. (nervenet.org)

We developed coisogenic bacterial artificial chromosome (BAC) transgenic mice harboring either the wild-type (G0), G1 or G2 forms of human APOL1. (omicsdi.org)

For the analysis of the linkage of quantitative traits, recombinant lines are useful because of their isogenic nature, because the genetic similarity of individuals allows for the replication of a quantitative trait locus analysis. (wikipedia.org)
Note to the Reader Please cite this work as: Williams RW, Gu J, Qi S, Lu L (2001) The genetic structure of recombinant inbred mice: High-resolution consensus maps for complex trait analysis. (nervenet.org)
Recombinant inbred (RI) strains of mice are an important resource used to map and analyze complex traits. (nervenet.org)
Recombinant inbred (RI) strains have been used to map a wide range of Mendelian loci and quantitative traits (Taylor, 1989). (nervenet.org)

40%), which enhances tumorigenesis in mouse models of CRC. (mendeley.com)
The study 'Cytotoxic necrotizing factor 1 hinders colon tumorigenesis induced by colibactin-producing Escherichia coli in ApcMin/+ mice' provides evidence that CNF1 decreases the carcinogenic effects of CoPEC in ApcMin/+ mice by decreasing CoPEC-induced cellular senescence and inflammation. (mendeley.com)

Our study was designed with an objective to interrogate the role of Pg and its fimbriae-mediated infection of human retinal-pigment epithelial cells and retro-orbitally injected mice retina, thus revealing possible molecular links between CP and AMD," said Hyun Hong, a predoctoral dental student. (dentistrytoday.com)
Our recent study reported that C3 opsonisation markedly increases the ability of E. coli strain J96 to internalise into human proximal tubular epithelial cells via CD46, a complement regulatory protein expressed on host cell membrane. (biomedcentral.com)
Studies from our group have shown that mice deficient in C3 are resistant to ascending infection and complement can alter bacterial uptake by mouse proximal tubular epithelial cells (PTECs), a primary target of E. coli during the acute phase of pyelonephritis [ 12 ]. (biomedcentral.com)

Thus, this study demonstrates that the transition metal zinc possesses antimicrobial properties against a wide range of GBS strains, with isolation source, capsular serotype, and sequence type contributing to susceptibility or resistance to zinc stress. (bvsalud.org)
E. coli strain J96 (serotype O4: K6) was provided by Dr. R. Welch, (University of Wisconsin, Madison, USA). (biomedcentral.com)

Additionally, there was no difference in vaginal burdens or persistence between COH1 and Δcfb strains in a murine colonization model. (bvsalud.org)
RESEARCH DESIGN AND METHODS -We established an insulin promoter-driven murine PD-L1 transgenic NOD mouse model to directly evaluate the protective effect of an organ-specific PD-L1 transgene against autoimmune diabetes. (diabetesjournals.org)

Tissues and bone marrow (BM)-derived monocytes were isolated from wild-type mice (WT) and from BAC/APOL1 transgenic (APOL1-G0, APOL1-G1, and APOL1-G2) mice, which carry a bacterial artificial chromosome that contains the human APOL1 genomic region. (omicsdi.org)

Inbred strains have been extensively used in research. (wikipedia.org)

Cell populations derived from these transgenic mice can be distinguished from syngeneic host and other mice with the alternate allele via flow cytometry. (ncard.org.au)

Most laboratory animals have the same set of organs - heart, lungs, liver, and so on which work in the same way as they do in human. (ommegaonline.org)
The majority of these were mice section or the collection of organs, tissues or cells. (drugstorepdfsearch.com)

A strain is inbred when it has undergone at least 20 generations of brother x sister or offspring x parent mating, at which point at least 98.6% of the loci in an individual of the strain will be homozygous, and each individual can be treated effectively as clones. (wikipedia.org)
homozygous nude mice lack T cells and suffer from a lack of cell-mediated immunity. (ncard.org.au)
Mice homozygous for the Rag1 tm1Mom mutation produce no mature T cells or B cells. (ncard.org.au)
Mice homozygous for the severe combined immune deficiency spontaneous mutation Prkdcscid, commonly referred to as scid, are characterized by an absence of functional T cells and B cells, lymphopenia, hypogammaglobulinemia, and a normal hematopoietic microenvironmentsevere combined immune deficient phenotype on Non obese diabetic genetic background. (ncard.org.au)

Certain plants including the genetic model organism Arabidopsis thaliana naturally self-pollinate, which makes it quite easy to create inbred strains in the laboratory (other plants, including important genetic models such as maize require transfer of pollen from one flower to another). (wikipedia.org)
Such strains are useful in the analysis of variance within an inbred strain or between inbred strains because any differences would be due to the single genetic change, or to a difference in environmental conditions between two individuals of the same strain. (wikipedia.org)
SGG UCN34 carrying the most prevalent T7SSb genetic arrangement was chosen as the reference strain. (pasteur.fr)

which is true of inbred strains, since they normally have at least 98.6% similarity by generation 20. (wikipedia.org)

Strain IGS type symbiotic efficiency Relating symbiotic functioning (measured here as specific nodule nitrogenase activity) to the IGS types found inside root nodules revealed significant differences in the N2-fixing efficiency of these IGS types (Figure 2). (trpvantagonists.com)

GBS possesses a broadly conserved small molecule, CAMP factor, that is co-hemolytic in the presence of Staphylococcus aureus sphingomyelinase C. While this co-hemolytic reaction is commonly used by clinical laboratories to identify GBS, the contribution of CAMP factor to GBS vaginal persistence is unknown. (bvsalud.org)
Common laboratory inbred mouse strains such as BALB/c and C57Bl6/J form the backbone of much of our pre-clinical work. (ncard.org.au)

CD8 TCR transgenic mouse whose CD8 T cells predominantly recognise the MHC class I (H-2K d ) restricted CL4 epitope of PR8 influenza HA protein. (ncard.org.au)
CD4 TCR transgenic mouse whose CD4 T cells predominantly recognise the MHC class II (I‑A d )‑restricted TCR specific for the PR8 influenza HA peptide 126-138 (HNTngvtaacshe) ( PMID: 7889402 ). (ncard.org.au)
Recessive, gain-of-function toxicity in an APOL1 BAC transgenic mouse model mirrors human APOL1 kidney disease. (omicsdi.org)

In a mouse model, AMD-related effects on mouse retinae were induced by Pg injected compared to a control group. (dentistrytoday.com)
Oral administration of PTC-672 reduces Ng infection in a mouse model and may have therapeutic potential for treatment of Ng that is resistant to current drugs. (elifesciences.org)
BALB/c based mouse model in which the administration of diphtheria toxin (DT) leads to specific depletion of regulatory T cells (Treg) due to expression of DT receptor-enhanced GFP under the control of the Foxp3 promoter. (ncard.org.au)
Asbestos induced mesothelioma mouse model in which expression of the Simian Virus 40 large T antigen (SV40 TAg) is controlled by mesothelin tissue‑specific promoter. (ncard.org.au)
I had decided to switch from rat to mouse as a model organism because of the advantage of using isogenic inbred strains for dissecting a multigene family, and the possibility of generating transgenic mice. (tatulo.pl)
Here, we report that the model organism SGG strain UCN34 secretes six T7SSb effectors. (pasteur.fr)

As a result, genomic structure, gene and protein expression and pathogenicity vary from strain to strain, potentially leading to differential roles in the CF lung. (marshall.edu)

NOD/SCID mice that received lymphocytes from transgenic mice became diabetic at a slower rate than mice receiving control lymphocytes. (diabetesjournals.org)

RESULTS: Invasive strains, such as those isolated from early onset disease manifestations of GBS infection were significantly less susceptible to zinc toxicity than colonizing strains isolated from rectovaginal swabs of pregnant patients. (bvsalud.org)
The 2003 EC proposal for the In the USA, laboratory animal use is federally reg- Registration, Evaluation and Authorisation of ulated by the Animal Welfare Act 1966 (amended in Chemicals (REACH), similarly aims to assess the 1985), which excludes laboratory-bred mice and toxicity of chemicals produced or imported in high rats, as well as non-mammals, from consideration quantities (15-20). (drugstorepdfsearch.com)
Neither heterozygous (G1/G0 or G2/G0) risk variant mice nor hemizygous (G1/-, G2/-) mice had significant kidney injury in response to IFN-γ, although the heterozygous mice had a greater proteinuric response than the hemizygous mice, suggesting that the lack of significant disease in humans heterozygous for G1 or G2 is not due to G0 rescue of G1 or G2 toxicity. (omicsdi.org)

BALB/c nude mice: A spontaneous mutation in Foxn1 nu induces abnormal hair growth (nude phenotype). (ncard.org.au)
The disease phenotype was greater in G2/G2 mice. (omicsdi.org)

BALB/cJ : a commonly used inbred mouse stain. (ncard.org.au)

A hybrid between two inbred strains can be used to cancel out deleterious recessive genes resulting in an increase in the mentioned areas. (wikipedia.org)
Zhihua Zou, another fellow, then made and analyzed mice that coexpressed the tracer with different OR genes. (tatulo.pl)

We found that an interneuron type that was previously associated with the mouse hippocampus-the 'ivy cell', which has neurogliaform characteristics-has become abundant across the neocortex of humans, macaques and marmosets but not mice or ferrets. (nature.com)
When the use of laboratory animal is obligatory, the most appropriate specie, breed, and strain with the closest homology to humans must be chosen. (ommegaonline.org)
Unlike humans mice do not host C. albicans in their mycobiome. (frontiersin.org)

In our preliminary studies of the SYNJ1 +/- mice, we found striatal dopamine depletion accompanied by increased levels of brain mTOR and DAT, suggesting a profoundly altered dopaminergic system. (rutgers.edu)

We assessed 30 strains of GBS from diverse isolation sources, capsular serotypes, and sequence types for susceptibility or resistance to zinc intoxication. (bvsalud.org)
Based on reported resistance of Ng strains, the CDC currently prescribes a two antibiotic protocol using ceftriaxone (a β-lactam) and azithromycin (a macrolide). (elifesciences.org)
Kurenbach B, et al demonstrate that bacteria may acquire antibiotic resistance in the environment at rates substantially faster than predicted from laboratory conditions. (gefree.org.nz)

We also found a notable subcortical innovation: an abundant striatal interneuron type in primates that had no molecularly homologous counterpart in mice or ferrets. (nature.com)
Fig. 4: A primate striatal interneuron type not observed in mouse or ferret. (nature.com)

Addtionally, alginate alone was enough to modify the ability to colonize a susceptible mouse lung, resulting in varied mortality and virulence. (marshall.edu)
Parasite virulence differs in stability in culture, with some strains attenuating more rapidly than others. (ap24534inhibitor.com)

One type of inbred strain that either has been altered, or naturally mutated so that it is different at a single locus. (wikipedia.org)
Additionally, capsular type III (cpsIII) strains and the ST-17 and ST-19 strains exhibited the greatest resilience to zinc stress, whereas ST-1 and ST-12 strains as well as those possessing capsular type Ib (cpsIb) were more sensitive to zinc intoxication. (bvsalud.org)

Inbred strains of animals are frequently used in laboratories for experiments where for the reproducibility of conclusions all the test animals should be as similar as possible. (wikipedia.org)
CONCLUSIONS -Our results demonstrate the protective potential of transgenic PD-L1 in autoimmune diabetes and illustrate its role in downregulating diabetogenic T-cells in NOD mice. (diabetesjournals.org)

According to Miller's 1958 laboratory notebooks, a few minutes after the experiment was initiated on March 24, 1958, a yellowing of the solution was observed, possibly from the formation of sulfur-bearing organic compounds or the polymerization of hydrogen cyanide (HCN). (pafr-signal.com)

First synapses are formed within the prenatal neocortices of both mice and human in two specialized zones, the marginal zone and subplate. (rutgers.edu)
Here we use single-nucleus RNA sequencing to profile RNA expression in 188,776 individual interneurons across homologous brain regions from three primates (human, macaque and marmoset), a rodent (mouse) and a weasel (ferret). (nature.com)

Biochemical, biophysical, and biological data support the efficacy of these molecules both in vitro and in mouse models. (elifesciences.org)

Tissue extracts of Mouse Testis (Lane 6) and Mouse Kidney (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
Kidney, spleen, and bone marrow-derived macrophages from APOL1-G1 and -G2 mice showed increased cholesterol accumulation and decreased ABCA1 and ABCG1 mRNA levels. (omicsdi.org)

RESULTS -The severity of insulitis in these transgenic mice is significantly decreased, disease onset is delayed, and the incidence of diabetes is markedly decreased compared with littermate controls. (diabetesjournals.org)

Immunodeficient mouse strains are invaluable when assessing the role of immune cell subsets in anti‑tumour immunity. (ncard.org.au)

Raw patient data used in the study were obtained from previous studies of laboratory. (mendeley.com)
Studies show that molecular profiling of NK603 corn and its isogenic control are not substantially equivalent. (gefree.org.nz)
We provide two-locus correlograms that can be used to detect and correct for non-syntenic association and to reduce error rates in QTL mapping studies that exploit RI strains. (nervenet.org)
Studies using additional mice (multicopy G2 and a non-isogenic G0 mouse) supported the notion that disease is largely a function of the level of risk variant APOL1 expression. (omicsdi.org)
Studies applying heparanase over-expressing and knock-out mice emphasize its ALK inhibitor role in tissue morphogenesis and as a master regulator of other ECM degrading enzymes. (pafr-signal.com)

BM-derived macrophages from APOL1-G1 and -G2 mice showed significantly reduced cholesterol efflux compared to WT or APOL1-G0 macrophages. (omicsdi.org)

P. aeruginosa small colony variants (SCVs) which prefer the biofilm mode of growth, thrive in harsh environmental conditions and are found in the chronically infected CF lung, were generated in the laboratory for further study. (marshall.edu)
APOL1 renal risk variants promote cholesterol accumulation in tissues and cultured macrophages from APOL1 transgenic mice. (omicsdi.org)

Raw data from mice and cells culture used in the study. (mendeley.com)
We investigated the protective potential of PD-L1 on autoimmune diabetes by transgenically overexpressing PD-L1 in pancreatic β-cells in nonobese diabetic (NOD) mice. (diabetesjournals.org)
Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)

Expression of interferon gamma (IFN-γ) via plasmid tail vein injection results in upregulation of APOL1 protein levels together with robust induction of heavy proteinuria and glomerulosclerosis in G1/G1 and G2/G2 but not G0/G0 mice. (omicsdi.org)

Several Nobel Prizes have been awarded for work that probably could not have been done without inbred strains. (wikipedia.org)
Ongoing work in Arjunan's laboratory could distinguish the specific causal role of Pg in AMD's pathogenesis. (dentistrytoday.com)

Moreover, lymphocytes collected from recipients transferred by lymphocytes from transgenic mice revealed less proliferative potential than lymphocytes obtained from control recipients. (diabetesjournals.org)
A total of 120 fish (75.31±0.24 g) were fed on a basal diet for 2 weeks to acclimatize the laboratory condition, then they were fed with diet containing B6 in 2 treatments of 5 and 10 mg B6 per kg of food and a control treatment (with commercial diet) for 3 weeks. (aejournal.ir)
Potential control of the infective stage of Taenia pisiformis using Bacillus thuringiensis GP526 strain. (uaem.mx)

Inbred strains (also called inbred lines, or rarely for animals linear animals) are individuals of a particular species which are nearly identical to each other in genotype due to long inbreeding. (wikipedia.org)
Some inbred strains have been bred for over 150 generations, leaving individuals in the population to be isogenic in nature. (wikipedia.org)
Genomes of individuals strains typically incorporate 45-55 recombinations between parental chromosomes. (nervenet.org)

Synthetic lethality was confirmed in both gastric and mammary organoid models of CDH1 loss, derived from CD44-Cre/Cdh1fl/fl/tdTomato mice. (ozgene.com)

To determine whether this deficiency is associated with wider vulnerabilities in vesicle trafficking, we screened isogenic MCF10A cell lines with known inhibitors of autophagy, endocytosis, and sphingolipid metabolism. (ozgene.com)

To test this hypothesis, we will use powerful combination of mouse genetics, polysome fractionation and CryoEM analysis. (rutgers.edu)

They were catalogued and identified by consulting Miller's original laboratory notebooks, which are kept in the Mandeville Special Nivolumab purchase Collections in the Geisel Library at the University of California, San Diego (Stanley L. Miller collection, Laboratory Notebook 2, page 114, Serial number 655, MSS642, Box 25, Mandeville Collections, Geisel Library). (pafr-signal.com)

Today, laboratories all over the world apply her techniques and insights to the sensory systems of all species, from the simplest to the most complex. (tatulo.pl)

Recently, RI strains have also been used to map QTLs that that are responsible for biometric variation in the architecture of the mouse CNS (Belknap et al. (nervenet.org)

One of the more specific uses of Drosophila inbred strains is the use of Gal4/UAS lines in research. (wikipedia.org)
These mice form the basis of the MexTAg research program . (ncard.org.au)

Homologous interneuron types-which were readily identified by their RNA-expression patterns-varied in abundance and RNA expression among ferrets, mice and primates, but varied less among primates. (nature.com)
Transgene expression, insulitis, and diabetic incidence were characterized in these transgenic NOD mice. (diabetesjournals.org)

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