• In the present study we aimed to determine the functional properties and the myosin heavy chain (MHC) isoform composition of single chemically skinned fibres from the vocal muscle of four adult men (age: 55-67 years). (unipd.it)
  • The myosin heavy chain (MyHC) isoforms are the best molecular markers of functional heterogeneity of muscle fibers. (diva-portal.org)
  • Zusätzlich bieten wir Ihnen Myosin Regulatory Light Chain 2, Smooth Muscle Isoform Kits (31) und Myosin Regulatory Light Chain 2, Smooth Muscle Isoform Proteine (15) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • We compared thermally induced denaturation and aggregation of two isoforms of the isolated myosin head (myosin subfragment 1, S1) containing different "essential" (or "alkali") light chains, A1 or A2. (mdpi.com)
  • In an attempt to understand the possible role of Na /H exchangers (NHEs) in uterine function, the mRNAs of different NHE isoforms as well as their subcellular localization (apical versus basolateral) and functional activity were investigated in mouse endometrial epithelial cells using reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and intracellular pH (pH i ) measurement techniques. (bioone.org)
  • Here we present a comprehensive study of the subcellular localization and trafficking of RET isoforms. (biomedsearch.com)
  • Differences in isoform-specific read densities indicated that a majority of AS and of alternative cleavage and polyadenylation (APA) events vary between tissues, while variation between individuals was ∼2- to 3-fold less common. (psu.edu)
  • A large number of isoforms are caused by single-nucleotide polymorphisms or SNPs, small genetic differences between alleles of the same gene. (wikipedia.org)
  • A protein isoform is a version of a protein with only small differences to another isoform of the same protein. (wikidoc.org)
  • To date, however, no systematic study has been undertaken to purify and characterize these isoforms with a view to establishing the biochemical basis for these differences. (springer.com)
  • To this end, we have utilized a novel method exploiting the differences in charge-based protein separation between isoelectrofocusing and ion exchange chromatography for the purification to homogeneity of the isoforms of human pituitary FSH. (springer.com)
  • These isoform-specific peptides will be invaluable tools to evaluate functional differences between localized RI and RII PKA and are RIalpha-specific disruptors. (sigmaaldrich.com)
  • Three different isoforms (named ALFLv1, ALFLv2 and ALFLv3) are observed, based on three nucleotide differences that produce three changes in amino acid sequence (Val11Ala, Val12Ala, Arg83Lys). (thefreedictionary.com)
  • In the present article, we review recent data defining the differences between the RAS isoforms and their most commonly mutated codons and discuss the underlying mechanisms. (portlandpress.com)
  • PFC overexpression of the IEG Homer1 isoform Homer1a reversed the genotypic differences in behavioral adaptation to repeated stress, whereas overexpression of the constitutively expressed Homer1 isoform Homer1c reversed the genotypic differences in sensorimotor and cognitive processing, as well as cocaine behavioral sensitivity. (jneurosci.org)
  • However, with proper electroosmotic flow (EOF) control, isoforms with small p I differences can also be analyzed using capillary zone electrophoresis (CZE). (wiley.com)
  • These findings support the conclusion that there are regiospecific and species-specific differences in the activities of P450 isoforms and that the inducibility of rat pulmonary P450s is dependent on the isoform and lung region. (aspetjournals.org)
  • Protein isoforms were detected using three data analysis strategies that evaluate differences between stable isotope labeling with amino acids in cell culture isotope ratios for specific groups of peptides within the total set of peptides assigned to a protein. (mcponline.org)
  • The content and composition of different vitamin E isoforms was analyzed in normal human skin. (thefreedictionary.com)
  • The goals of this study were to explore whether this differing association of vitamin E on asthma risk is due to an interaction of vitamin E isoforms. (bmj.com)
  • To address this question, in a population-based asthma incidence study we assessed the interaction between the plasma concentrations of vitamin E isoforms α-tocopherol and γ-tocopherol on asthma risk. (bmj.com)
  • These human and animal studies provide evidence for the competing effects of the vitamin E isoforms, in physiological concentrations, on asthma and allergic airway disease. (bmj.com)
  • 1997) this pattern was explained as the result of regional duplication ('in tandem') occurring at the sMDH-A*. While these sMDH-A* loci encode nondivergent thermostable isoforms, the sMDH-B* encodes a thermolabile one. (scielo.br)
  • The donor site for the short isoform (P4.2S) agrees better with the consensus than the donor site for the long isoform (P4.2L) does. (bloodjournal.org)
  • A concluding chapter looks at the possible future clinical role of the TGF-beta isoform in human studies. (thefreedictionary.com)
  • Separation of isoforms of the glycoprotein hormones from human pituitary extracts. (springer.com)
  • Stanton P.G., Robertson D.M., Burgon P.G., White B., Hearn M.T.W. (1992) Purification and Biological Activities of Isoforms of Human FSH. (springer.com)
  • Pegaptanib sodium (Macugen), which is currently approved for the treatment of macular degeneration, works by specifically blocking one of the six human isoforms of vascular endothelial growth factor (VEGF). (thefreedictionary.com)
  • Human, rat and mouse cAMP phosphodiesterase isoforms (denoted PDE4D7s), as well as the DNA (RNA) encoding such polypeptides, are disclosed. (freepatentsonline.com)
  • Recombinant Human NRG1 Isoform SMD. (novusbio.com)
  • Scientists led by Radosveta Koldamova at the University of Pittsburgh, Pennsylvania, reported that a depletion of the ATP-binding cassette transporter A1 (ABCA1)-which shuttles lipids to ApoE-fans the flames of cognitive problems and Aβ deposition in transgenic mice carrying the human ApoE4 gene, but not in animals with the ApoE3 isoform. (alzforum.org)
  • To add to its complexity, several isoforms of human p53 have now been reported. (bloodjournal.org)
  • Ten isoforms of human p53 have been reported that are generated by the use of alternative translation initiation sites, splicing, or alternative promoters. (bloodjournal.org)
  • Bovine thyroglobulin-conjugated synthetic peptide corresponding to amino acids 275-291 (VQIINKKLDLSNVQSKC) of human Tau (4-repeat isoform RD4). (fishersci.com)
  • Despite the lack of stable snRNA orthologous groups during evolution, this developmental switching of snRNA isoforms also occurs in distantly related vertebrate species, such as Xenopus , mouse, and human. (g3journal.org)
  • All 3 isoforms of the protein could be localized in human ventricular myocytes with fluorescence immunohistochemistry. (ahajournals.org)
  • Conclusions -All 3 isoforms of the SR Ca 2+ -release channel were determined in human ventricular cardiomyocytes. (ahajournals.org)
  • Isoforms of transferrin and α 1 -antitrypsin have been implicated in several human diseases. (wiley.com)
  • We used gene targeting to create isoform-specific Fbw7-null mutations in human cells and found that the nucleoplasmic Fbw7α isoform accounts for almost all Fbw7 activity toward cyclin E, c-Myc, and sterol regulatory element binding protein 1. (rupress.org)
  • To clarify these issues, we used a gene-targeting approach using adeno-associated virus (AAV) vectors to create a series of homozygous isoform-specific Fbw7-null mutations in human colon carcinoma cells. (rupress.org)
  • Taken together, our data indicate that murine mast cells express novel, fully functional IL-15Rα isoforms, which can explain the selective regulatory effects of IL-15 on these cells. (jimmunol.org)
  • The possibly duplicated A2 isoform showed higher substrate affinity than the A1. (scielo.br)
  • Importantly, IL-15 binds with high affinity to, and promotes the survival of, murine BA/F3 cells stably transfected with the IL-15Rα isoforms. (jimmunol.org)
  • By coupling the CZE isoform separation with standard affinity capture assays, it may be possible to develop a cost-effective analytical platform for clinical diagnostics. (wiley.com)
  • CONCLUSIONS: The severity of this patient's phenotype may be due to overexpression of the exon 31-spliced laminin alpha2 isoform. (biomedsearch.com)
  • Because Homer1 gene deletion eliminates both IEG and CC-Homer products, we infused adeno-associated viral vectors (AAVs) carrying either Homer1a or Homer1c into the PFC to address the relative role for these different Homer1 isoforms in the "schizophrenia-like" behavioral and neurochemical phenotype of Homer1 KO mice. (jneurosci.org)
  • The distribution of pulmonary cytochrome P450 (P450 or CYP) isoforms has been investigated primarily in immunohistochemical studies, which are neither quantitative nor reflective of the functions of these enzymes. (aspetjournals.org)
  • Furthermore, the short isoform behaved as a putative tumor suppressor based on cell proliferation and cell colony formation assays. (thefreedictionary.com)
  • Objective- The purpose of this study was to test the function of the calcium/calmodulin-dependent protein kinase II δ 2 isoform (CaMKIIδ 2 ) in regulating vascular smooth muscle (VSM) cell proliferation and migration in response to vascular injury. (ahajournals.org)
  • We hypothesized that \(\beta\)-cell proliferation and glucose homeostasis were controlled by \(\Delta\)40p53, a p53 isoform lacking the transactivation domain of the full-length protein that modulates total p53 activity and regulates organ size and life span in mice. (harvard.edu)
  • The network responses associated with each isoform and individual oncogenic mutations remain to be fully characterized. (portlandpress.com)
  • Importantly, all three RAS isoforms share complete sequence identity at these sites and the mutations are assumed to have equivalent effects on the activity of each isoform [ 4 ]. (portlandpress.com)
  • Jinesh GG, Laing NM, Kamat AM (2016) Smac mimetic with TNF-alpha targets Pim-1 isoforms and reactive oxygen species production to abrogate transformation from blebbishields. (springer.com)
  • 1 2 The original 17 kDa isoform of IL1Ra is secreted as variably glycosylated species of 22-25 kDa from monocytes, macrophages, neutrophils, hepatocytes, microglial cells, and other cells. (bmj.com)
  • To evaluate the effects of each localized isoform, we designed peptides that specifically bind to either RI or RII. (sigmaaldrich.com)
  • Oxidative ability towards aromatic compounds varied substantially among the isoforms and each of them displayed preference toward some substrates. (mdpi.com)
  • In the generation of ICAM-1-deficient mice, two isoform-deficient ICAM-1 mutants were inadvertently produced as a result of alternative splicing. (jimmunol.org)
  • These mice, along with true ICAM-1-deficient mice and newly generated ICAM-1-transgenic mice, have provided the opportunity to begin examining the role of ICAM-1 isoforms (singly or in combination) in various disease settings. (jimmunol.org)
  • In this review, we highlight the sharply contrasting disease phenotypes using ICAM-1 isoform mutant mice. (jimmunol.org)
  • Second, to understand the mechanisms of any interaction of these isoforms, we conducted experimental supplementation of α-tocopherol and γ-tocopherol isoforms in mice on the outcome of allergic airway inflammation. (bmj.com)
  • The systematic analysis of lupus-prone congenic mouse strains suggests a role for two isoforms of the Ly108 receptor in the pathogenesis of the disease. (rupress.org)
  • The p110α isoform of PI3K (encoded by Pik3ca ) is an essential effector of oncogenic KRAS in murine lung tumors, but it is unknown whether p110α contributes to leukemia. (jci.org)
  • This differential trafficking of RET isoforms produces a more rapid and longer duration of signaling through the extracellular-signal regulated kinase/mitogen-activated protein kinase pathway downstream of RET51 relative to RET9. (biomedsearch.com)
  • Laminin alpha2 immunofluorescence showed that this isoform was not evenly distributed at the muscle fiber basal lamina, but preferentially localized in discrete areas. (biomedsearch.com)