KinaseLactateMetabolismReceptorsPeptidesPhosphorylationEnzymeCell wall proteinsCreatineElectrophoretic analysisMiceElectrophoresisAlkalinePolysaccharidesMicrotubule-associaBindingModulatePathwayCellularPhospholipasePeptideDistinctSynthesisInitiatesTissuePatternsCytoplasmicLiverMuscleDiagnoseDamagesTotalHumanConcentrationDeterminationInvestigatorsCellsTest measures
Kinase17
- abstract = 'Conventional (c) protein kinase C (PKC) activity has been shown to increase with skeletal muscle contraction, and numerous studies using primarily pharmacological inhibitors have implicated cPKCs in contraction-stimulated glucose uptake. (ku.dk)
- AMP-activated protein kinase (AMPK) and eukaryotic elongation factor 2 phosphorylation was unaffected by the blockers. (ku.dk)
- Sequential activation of protein kinase C isoforms by organic dust is mediated by tumor necrosis factor. (cdc.gov)
- This response involves the activation of two protein kinase C (PKC) isoforms: PKCa and PKCe. (cdc.gov)
- Genetic analysis of the role of protein kinase Ctheta in platelet function and thrombus formation. (cam.ac.uk)
- BACKGROUND: PKCtheta is a novel protein kinase C isozyme, predominately expressed in T cells and platelets. (cam.ac.uk)
- Scholars@Duke publication: Protein kinase C beta II specifically binds to and is activated by F-actin. (duke.edu)
- The two most closely related isoenzymes of protein kinase C (PKC), PKC betaI and betaII, are distinct but highly homologous isoenzymes derived via alternative splicing of the same gene product. (duke.edu)
- At 2 and/or 6 days, we evaluated creatine kinase (CK and CK-MB), cardiac troponin I (cTnI), C-reaction protein (CRP), serum tumor necrosis factor (TNF- α ), serum creatinine (Cr), serum urea, glucose, glutamic pyruvic transaminase (ALT), and glutamic oxaloacetic transaminase (AST). (hindawi.com)
- The expression of protein kinase C (PKC) isoenzymes and the effects of PKC activation on myocardial phospholipase A2 (PLA2) activity, platelet-activating factor (PAF) generation and eicosanoid release were studied in spontaneously beating cultured rat cardiomyocytes. (unige.ch)
- The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)
- Creatine Kinase-MB Isoenzyme Protein, clude purifed Creatine Kinase (CK) is a dimeric enzyme comp. (kitairu.net)
- Studies using targeted gene deletion in mice have revealed distinct roles for individual isozymes of the protein kinase C (PKC) family of enzymes in regulating sensitivity to various drugs of abuse. (elsevierpure.com)
- Olive, MF & Newton, PM 2010, ' Protein kinase C isozymes as regulators of sensitivity to and self-administration of drugs of abuse-studies with genetically modified mice ', Behavioural pharmacology , vol. 21, no. 5-6, pp. 493-499. (elsevierpure.com)
- Phospholipase D activity in human platelets is inhibited by protein kinase A, involving inhibition of phospholipase D1 translocation. (uib.no)
- Recruitment of coactivator glucocorticoid receptor interacting protein 1 to an estrogen receptor transcription complex is regulated by the 3',5'-cyclic adenosine 5'-monophosphate-dependent protein kinase. (uib.no)
- A casein kinase I isoenzyme with specificity for proteins involved the regulation of the CIRCADIAN RHYTHM. (bvsalud.org)
Lactate5
- This test measures lactate dehydrogenase (LDH) isoenzymes in a sample of your blood. (medlineplus.gov)
- lactate dehydrogenase isoenzymes were separated by agarose gel electrophoresis and quantified. (avma.org)
- The percentage contributions of lactate dehydrogenase 1 and 2 isoenzymes to the total amount of all 5 isoenzymes at 12 and 24 months of age were significantly higher than those at 2 months of age. (avma.org)
- In addition, a significant decrease in the activities of myocardial injury markers i.e., creatine phosphokinase-MB (CK-MB isoenzyme) and lactate dehydrogenase (LDH) was also observed in the heart of ISP control group as compared to sham control. (scialert.net)
- Other biological markers for germ cell tumors include lactate dehydrogenase isoenzymes and placental alkaline phosphatase, although these are less specific. (medscape.com)
Metabolism1
- Protein phosphatases reverse the covalent modifications of numerous cellular proteins imposed by the activation of protein kinases and, therefore, play key role in cell signaling metabolism, growth and differentiation. (bvsalud.org)
Receptors1
- It is suggested that G-protein-coupled receptors might modulate cytoskeletal dynamics, intracellular traffic, and cellular architecture. (jneurosci.org)
Peptides1
- Therefore, for each protein, heavy labeled reference peptides (selected from the shotgun LC-MS/MS experiment) were synthetized. (harvard.edu)
Phosphorylation2
Enzyme2
- An enzyme is a protein that speeds up certain chemical reactions in your body. (medlineplus.gov)
- There are five forms of the LDH enzyme which are called LDH isoenzymes. (medlineplus.gov)
Cell wall proteins2
- This Special Issue, "Plant Cell Wall Proteins and Development", will cover a selection of recent research topics in the field of cell wall biology focused on cell wall proteins and their roles during development. (mdpi.com)
- They mainly comprise polymers like polysaccharides and lignin in lignified secondary walls and a minute amount of cell wall proteins (CWPs). (mdpi.com)
Creatine1
- The creatine phosphokinase (CPK) isoenzymes test measures the different forms of CPK in the blood. (adam.com)
Electrophoretic analysis1
- Twenty-one species belonging to 13 genera of Acanthaceae obtained from Saudi Arabia were studied using electrophoretic analysis for protein patterns and two isoenzymes polymorphism in addition the phytochemical screening by TLC and HPLC. (scholarsresearchlibrary.com)
Mice2
- To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
- These mice express a catalytically inactive fusion protein of the NH 2 -terminal part of HDAC2 and β-galactosidase, which fails to integrate into corepressor complexes with mSin3B. (aacrjournals.org)
Electrophoresis1
- 7 ]. Seed protein and isozyme electrophoresis have been the most widely employed molecular genetic markers during the last quarter century [ 8 , 9 ]. (scholarsresearchlibrary.com)
Alkaline1
- The purpose of this study was to investigate the activities of the total acid phosphatase (TAP), tartrate-resistant acid phosphatase (TRAP), low molecular weight protein tyrosine phosphatase (LMW-PTP) and alkaline phosphatase (ALP) enzymes, as well as the possible correlation in the serum and in unstimulated whole saliva of children. (bvsalud.org)
Polysaccharides1
- Cell wall proteomics, as well as numerous genetic or biochemical studies, have revealed the high diversity of CWPs, among which proteins acting on polysaccharides, proteases, oxido-reductases, lipid-related proteins and structural proteins. (mdpi.com)
Microtubule-associa1
- Protein tau is a major microtubule-associated protein in the vertebrate nervous system. (europa.eu)
Binding3
- The interaction of tubulin with these polypeptides involves a GTP transfer from the exchangeable GTP-binding site (E site) of tubulin to Gα, which activates the G-protein (transactivation) ( Roychowdhury and Rasenick, 1994 ). (jneurosci.org)
- Complex patterns of posttranslational modifications of chromatin including acetylation of histones establish combinatorial signals that are apparently interpreted by chromatin-binding proteins and the gene expression machinery to control transcriptional activity of individual genes. (aacrjournals.org)
- Transglutamina-se(TGase) catalyses the crosslink of proteins by forming ε-(γ-glutamyl) lysine isopeptide bonds and requires the binding of Ca2+ for its activity. (biovendor.com)
Modulate1
- Histone deacetylases (HDAC) reverse the acetylation of histone and nonhistone proteins and thereby modulate chromatin structure and function of nonhistone proteins. (aacrjournals.org)
Pathway3
- Second, the cellular concentrations of 41 proteins covering all components of the glycolysis pathway were determined across all samples by SRM and SID [see comments section]. (harvard.edu)
- Specifically, [they] selected the enzymes and iso-enzymes of the glycolytic pathway (including proteins with hypothetical function), tricarboxylic acid cycle enzymes and a few other proteins (Supplementary Table 1). (harvard.edu)
- Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway. (uib.no)
Cellular3
- Translocation of PKC alpha, delta and zeta was accompanied by simultaneous increases in cellular lysophosphatidylcholine (lyso-PC), PAF, 15(S)-hydroxy-5,8,-11,13-eicosatetraenoic acid (15-HETE), prostaglandin E2 (PGE2) and prostacyclin (PGI2) generation, suggesting that one or more of these isoenzymes directly or indirectly activates a PLA2 in these cells. (unige.ch)
- With this information, the protein concentrations determined by SRM and/or SID could be transformed to protein copies/cell and a quantitative model was built to translate MS intensities of all quantified proteins to cellular abundance estimates using the Intensity Based Absolute Quantification (iBAQ) approach [ref 4]. (harvard.edu)
- The protein composition of saliva also reflects cellular signal processing that results from day-to-day environmental influences, as well as from acute or chronic stress 9 . (bvsalud.org)
Phospholipase2
- The microtubule protein tubulin regulates adenylyl cyclase and phospholipase Cβ 1 (PLCβ 1 ) signaling via transactivation of the G-protein subunits Gαs, Gαi1, and Gαq. (jneurosci.org)
- Phospholipase D in human platelets: presence of isoenzymes and participation of autocrine stimulation during thrombin activation. (uib.no)
Peptide1
- In the Alzheimer's disease brain, the elevated tTGase activity is manifested by polymerization of a number of proteins, including Aβ peptide, β-amyloid precursor protein and the tau protein, with formation of neurofibrillary tangles. (biovendor.com)
Distinct1
- In mammals, eight distinct TGase isoenzymes have been identified. (biovendor.com)
Synthesis5
- Vault offers the following treatments as part of their testosterone replacement records Ecdysterone for sale of their inventory and transactions interfering with human monocyte promote protein synthesis is second to none. (zapthink.com)
- This was accompanied by a 2.8-fold increase in COX-2 protein level and a 1.5-fold increase in PGE(2) synthesis. (duke.edu)
- The estrogen receptor antagonist ICI 182,780 fully reversed the stimulation of COX-2 mRNA and protein levels and PGE(2) synthesis by E(2). (duke.edu)
- Interestingly, estrogen receptor beta mRNA and protein were abundant in HUMEC, whereas estrogen receptor alpha mRNA or protein was barely detectable.We conclude that various levels of E(2) can significantly increase COX-2 expression and PGE(2) synthesis in HUMEC via the estrogen receptor. (duke.edu)
- 2008). Abolition of stress-induced protein synthesis sensitizes leukemia cells to anthracycline-induced death. (uib.no)
Initiates1
- In this regard, TMZ may protect cardiomyocytes against excessive oxygen free radical (OFR) production that occurs in ischemia and inflammation and that initiates oxidation of lipids and proteins and damages DNA. (hindawi.com)
Tissue3
- Because each LDH isoenzyme is found in more than one type of tissue, other more specific tests are often used with or instead of an LDH isoenzymes test. (medlineplus.gov)
- CPK isoenzyme testing can help find the exact source of the damaged tissue. (adam.com)
- PGP9.5 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme. (neuromics.com)
Patterns1
- 2003). The comparison of electrophoretic protein patterns has been considered as a method with satisfactory taxonomic resolution, which can be applicable to the level of species, subspecies and biotypes (Caugant and Sandven, 1993). (scialert.net)
Cytoplasmic3
- PGP9.5 is a soluble cytoplasmic protein with a molecular weight of approximately 25,000 kD. (neuromics.com)
- After cell wall disruption, the cell wall and cytoplasmic proteins were submitted to PAGE and SDS-PAGE techniques. (scialert.net)
- In this study, we described the use of SDS-PAGE, along with PAGE techniques, to analyze the similarity of the protein profiles of the cytoplasmic extracts and cell walls of different C. albicans isolates obtained from different host conditions (human against animal isolates and patient against healthy subject isolates) with various virulence degrees. (scialert.net)
Liver1
- DxD primarily metabolized by the liver via CYP3A4 isoenzyme. (unboundmedicine.com)
Muscle1
- PKCalpha was estimated to account for approximately 97% of total cPKC protein expression in skeletal muscle. (ku.dk)
Diagnose3
- But an LDH isoenzymes test alone cannot diagnose what's causing the damage. (medlineplus.gov)
- An LDH isoenzyme test may be used with other tests to help diagnose and monitor many types of acute (sudden) and chronic (long-lasting) conditions. (medlineplus.gov)
- A significant rise or fall in the total CPK or CPK isoenzymes can help your health care provider diagnose certain conditions. (adam.com)
Damages1
- But if disease or injury damages tissues that contain LDH, the cells release LDH isoenzymes into your bloodstream and your LDH levels will rise above normal. (medlineplus.gov)
Total1
- A total number of twenty-three protein bands observed between the species ranged 10 and 250 K Da, nine esterase and six peroxidase polymorphisms were detected. (scholarsresearchlibrary.com)
Human3
- SARS-CoV-2 spike and nucleocapsid proteins fail to activate human dendritic cells or "" T cells. (tcd.ie)
- The aim of the study was to evaluate the protein similarity degree among 15 C. albicans isolates with different virulence obtained from healthy and infected human and animals. (scialert.net)
- Such results suggest a highly protein similarity degree among the most isolates with low and high virulence as well as from human and animal hosts. (scialert.net)
Concentration1
- The concentration range of the 41 proteins covered more than four orders of magnitudes ranging from around 92,000 (Mdh, on acetate medium) to only 2 (YbhA, 3 d into stationary phase) copies per cell. (harvard.edu)
Determination1
- P.2 caption to figure 1:'Workflow of system-wide protein abundance determination: The workflow comprised three steps. (harvard.edu)
Investigators1
- P.2 left column 2nd paragraph:'Second, [investigators] accurately quantified a subset of identified proteins to establish a 'calibration' for the MS [mass spectrometry] intensities determined for all identified proteins. (harvard.edu)
Cells1
- First, cells of the various samples were lysed and proteins extracted and proteolyzed using trypsin. (harvard.edu)
Test measures1
- An LDH isoenzyme test measures the amount of each type of isoenzyme in your blood. (medlineplus.gov)