Filter...

AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceHealth Care

Select a category...

Electrophoresis, Polyacrylamide GelGelsMolecular WeightElectrophoresisElectrophoresis, Gel, Two-DimensionalElectrophoresis, CapillaryElectrophoresis, Agar GelElectrophoresis, DiscElectrophoresis, Gel, Pulsed-FieldIsoelectric FocusingChromatography, GelAmino AcidsKineticsIsoelectric PointSodium Dodecyl SulfateMolecular Sequence DataChromatography, AffinityImmunodiffusionChromatography, Ion ExchangeMacromolecular SubstancesHydrogen-Ion ConcentrationAmino Acid SequenceImmunoelectrophoresisElectrophoresis, MicrochipAcrylic ResinsChromatographyChromatography, DEAE-CelluloseBlood Protein ElectrophoresisBase SequenceElectrophoresis, Starch GelEscherichia coliCentrifugation, Density GradientBacterial ProteinsSubstrate SpecificityChemistryGlycoproteinsChemical PhenomenaCattleRabbitsCarbohydratesElectrophoresis, Cellulose AcetateChemical PrecipitationSpecies SpecificityTrypsinPeptidesProteinsDensitometryPeptide FragmentsAcrylatesAcrylamidesImmunochemistryDNA, BacterialDNAProtein BiosynthesisLiverImmunoelectrophoresis, Two-DimensionalTemperatureDenaturing Gradient Gel ElectrophoresisCell LineViral ProteinsHot TemperatureUltracentrifugationMicroscopy, ElectronPolymerase Chain ReactionImmune SeraMembrane ProteinsCell MembraneCell FractionationCell-Free SystemIsoenzymesHydroxyapatitesImmunosorbent TechniquesChromatography, High Pressure LiquidMethodsTritiumProtein BindingCloning, MolecularAutoradiographyProteomicsPeptide HydrolasesPeptide BiosynthesisRosaniline DyesNative Polyacrylamide Gel ElectrophoresisBlood ProteinsSolubilityCross ReactionsNucleic Acid DenaturationMutationAmmonium SulfateSwineChickensSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationProteomeDetergentsMercaptoethanolImmunologic TechniquesAffinity LabelsSpectrophotometryRNA, ViralSepharose

Dimensional Polyacrylamide Gel ElectrophorFocusingSodiumProteins can be separatedImmobilized pH gradientsPointChromatographyAntigensElectrophoreticGradientDimensionPAGESeparationReactionBufferMobilityPatternsAnalysisInvestigateBasisDrug-sensitiveStepChargePointsFormsMassHighlyMainResultsSystemTableSolution

Dimensional Polyacrylamide Gel Electrophor5

• Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. (hindawi.com)
• Two-dimensional polyacrylamide gel electrophoresis of the two molecular weight classes of enzyme has shown that each is composed of two isoelectric forms, and that the relative distributions of the isoelectric forms within a molecular weight class are maintained during overproduction. (researchwithrutgers.com)
• An analytical reference map of normal human reflex tears was created using two- dimensional polyacrylamide gel electrophoresis (2-D PAGE) with pH 3.5-10 immobilized pH gradients (IPGs). (edu.au)
• Excessive decision two-dimensional electrophoresis of proteins A way has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. (stat-2.com)
• Using two-dimensional polyacrylamide gel electrophoresis and immunoblot, we found that there are two major PHI-1 spots under resting conditions: a minor spot with an acidic isoelectric point (pI) and a major spot with a more alkaline pI. (uky.edu)

Focusing9

• Proteins can, thus, be separated by net charge in a polyacrylamide gel using either preparative gel electrophoresis, which uses a constant pH to separate proteins or isoelectric focusing, which uses a pH gradient to separate proteins. (wikipedia.org)
• Isoelectric focusing is also the first step in 2-D gel polyacrylamide gel electrophoresis. (wikipedia.org)
• Isoelectric Focusing 11. (nphindia.com)
• The enzyme was pure as judged by polyacrylamide gel electrophoresis with and without sodium dodecyl sulphate as well as by isoelectric focusing. (rhea-db.org)
• Polyacrylamide gel electrophoresis studies and isoelectric focusing indicate that this membrane bound binding site is a protein with a molecular weight of 70-85 kDa and an isoelectric point of ~7. (nova.edu)
• We obtained about 1000 protein spots that were highly reproducible across an isoelectric focusing range 4-7. (unimol.it)
• A further peptidase (aminopeptidase M') was resolved by isoelectric focusing.in polyacrylamide gels. (keele.ac.uk)
• The predicted amino acid sequence yields a protein mass of 30,200 Da, but on polyacrylamide gel electrophoresis or size exclusion chromatography it has an apparent molecular mass of 32-38 kDa and by isoelectric focusing a PI of about 3.5 - 4.0 depending on species and technique. (pancreapedia.org)
• Others have shown that human and bovine DNase 1 could be separated into 4 - 8 bands on isoelectric focusing (37). (pancreapedia.org)

Sodium3

• their molecular masses were determined to be 52.5 and 54 kDa, respectively, by observation of the mobility of the protein bands in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (wikigenes.org)
• Solubilisation of brush border membrane proteins by sodium dodecyl sulphate, Triton X-100 and papain followed by polyacrylamide gel electrophoresis revealed seven different peptide hydrolases. (keele.ac.uk)
• Salivary protein profiles of 43 patients and 39 healthy control subjects were compared on three different gel electrophoresis systems: sodium dodecylsulfate-polyacrylamide gel electrophoresis, anionic polyacrylamide gel electrophoresis, and immobilized pH gradient gel electrophoresis (isoelectric point, 3.5-5.0). (elsevierpure.com)

Proteins can be separated1

• Protein Electrophoresis is a method in which a mixture of proteins can be separated and analyzed. (wikibooks.org)

Immobilized pH gradients1

• Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. (sandia.gov)

Point9

• Proteins are separated in the first dimension based on their isoelectric point, and then in the second dimension by molecular weight. (learner.org)
• The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. (wikipedia.org)
• The standard nomenclature to represent the isoelectric point is pH(I). However, pI is also used. (wikipedia.org)
• In the two examples (on the right) the isoelectric point is shown by the green vertical line. (wikipedia.org)
• In fact the concentration of (AMP)H2+3 is negligible at the isoelectric point in this case. (wikipedia.org)
• this enables the movement of a protein molecule towards the isoelectric point at which the molecule has no net charge. (sigmaaldrich.com)
• The isoelectric point was 8.4. (rhea-db.org)
• Two-dimensional gel electrophoresis showed that one spot with a molecular mass 20 kDa and an isoelectric point of 6.0, became significantly smaller after immortalization of human cells. (elsevierpure.com)
• The isoelectric point of this enzyme was 3.6 and it was stable at temperatures below 45 °C over a wide range of pH (4-12). (ncsu.edu)

Chromatography2

• The enzyme was shown to have a molecular weight of about 450,000 by gel chromatography. (rhea-db.org)
• 1994). The molecular weight (MW) of MTGase was shown to be about 38 kDa in both SDS-polyacrylamide gel electrophoresis and gel-permeation chromatography. (zedira.com)

Antigens1

• Simplification of two-dimensional gel patterns of HLA class II antigens. (ox.ac.uk)

Electrophoretic3

• p I = p K a 1 + p K a 2 2 {\displaystyle \mathrm {pI} ={\frac {\mathrm {p} K_{\mathrm {a1} }+\mathrm {p} K_{\mathrm {a2} }}{2}}} The pH of an electrophoretic gel is determined by the buffer used for that gel. (wikipedia.org)
• After electrophoretic run immerse the gel in fixing solution for 40 min. (sigmaaldrich.com)
• gradient gel electrophoretic methods enabled a distinction to be made between coniferyl alcohol oxidase (cao) of lignifying cell walls and a pi approximately 9 pine 'laccase' recently implicated in lignification (science 1993 260, 672). (liverpool.ac.uk)

Gradient2

• On the basis of its mobility on native gradient gel, the glucosyltransferase was estimated to have a molecular mass of 440 kDa. (wikigenes.org)
• Among the three gel electrophoresis systems examined, the immobilized pH gradient system appeared to be the most reliable for Sjögren's syndrome. (elsevierpure.com)

Dimension1

• The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. (sandia.gov)

PAGE5

• The most common method is two-dimensional polyacrylamide gel electhrophoresis (2D-PAGE). (hindawi.com)
• Coomassie Blue staining: Staining of protein gels with Coomassie Brilliant Blue R-250 is a common procedure to visualize proteins resolved by SDS-PAGE. (sigmaaldrich.com)
• We offer a highly sensitive protein detection silver stain kit suitable for SDS-PAGE gels. (sigmaaldrich.com)
• Using twodimensional polyacrylamide gel electrophoresis (2D-PAGE) we compared the protein patterns of S. junceum roots grown in a greenhouse in either upright or tilted pots. (unimol.it)
• Thirteen fungal isolates included in this study expressed multiple xylanase isoforms as observed by xylan zymograms of polyacrylamide gel electrophoresis (PAGE) and isoelectrofocussing (IEF) fractionated proteins. (ncsu.edu)

Separation3

• The charge distribution of the molecules is critical in the separation of all electrophoresis. (wikibooks.org)
• The separation of the protein molecules in the gel affects the protein activity. (wikibooks.org)
• The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices. (sandia.gov)

Reaction2

• Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide ( N,N '-methylenebisacrylamide) that results in highly cross-linked gel matrix. (sigmaaldrich.com)
• We discovered that these proteins formed gels by the reaction with TGase in highly concentrated solutions, e.g. soybean 11S globulin 10%, 7S globulin 10%, and αS1-casein 5% (Fig.1). (zedira.com)

Buffer1

• If the pH of the buffer is below the pI of the protein being run, the protein will migrate to the negative pole of the gel (positive charge is attracted to the negative pole). (wikipedia.org)

Mobility1

• Electrophoresis is based on the mobility of ions in an electric field. (wikibooks.org)

Patterns1

• Two-dimensional gel electrophoresis of material immunoprecipitated from radiolabeled cells by anti-HLA class II monoclonal antibodies has been a useful technique but the patterns observed have varied considerably between laboratories. (ox.ac.uk)

Analysis1

• Micropreparatively loaded gels were transferred to polyvinylidene difluoride (PVDF) and analysed by a combination of N-terminal sequence tagging and amino acid compositional analysis. (edu.au)

Investigate2

• Adherence of P. gingivalis strains to serum-coated surfaces was studied with confocal microscopy and 2D-gel electrophoresis of bacterial supernatants used to investigate extracellular proteins. (biomedcentral.com)
• The purpose of this study was to investigate the potential use of salivary electrophoresis for the diagnosis of Sjögren's syndrome. (elsevierpure.com)

Basis1

• Xylanases identified on the basis of their isoelectric points (pI) were functionally diverse and exhibited differential catalytic activities against various xylan types (birch wood xylan, larch wood xylan, oat spelt xylan, rye arabino xylan and wheat arabino xylan) as well as debranched arabinan. (ncsu.edu)

Drug-sensitive1

• Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)

Step1

• After the staining step, wash the gel several times with distilled water to remove excess stain. (sigmaaldrich.com)

Charge1

• Gel electrophoresis is performed to analyze the molecular weights and the charge of the protein and is mostly used in electrophoresis of the protein. (wikibooks.org)

Points1

• A number of algorithms for estimating isoelectric points of peptides and proteins have been developed. (wikipedia.org)

Forms3

• The crosslinked of acrylamide and N,N'-methylene-bis-acrylamide forms the polyacrylamide by polymerization. (wikibooks.org)
• In vitro translation experiments have demonstrated that the synthesis of these isoelectric forms and their relative distributions are mediated by mRNA. (researchwithrutgers.com)
• Both sets, within a subline, encode the same molecular weight class of dihydrofolate reductase, while each set directs the synthesis of only one of the isoelectric forms within that class. (researchwithrutgers.com)

Mass1

• Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins were identified by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry. (who.int)

Highly1

• It is highly sensitive and is suitable for long-term storage of the gels. (sigmaaldrich.com)

Main1

• The polyacrylamide gel electrophoresis of purified laccase showed a main polypeptide with a molecular weight of about 60 kDa. (ncsu.edu)

Results1

• Our results suggest that salivary electrophoresis is a potentially useful test for the diagnosis of Sjögren's syndrome. (elsevierpure.com)

System1

• Activity recovered in the aminopeptidase M peak in the above gel system could be resolved into two distinct peptidases in addition to aminopeptidase M, by SDS-gel electro-phoresis. (keele.ac.uk)

Table1

• Place on a rocking table and stain the gel for 2-4 hours. (sigmaaldrich.com)

Solution14

• In an electric field, electrophoresis is a passage of charged molecules in solution. (wikibooks.org)
• Prepare resolving gel solution using the following volumes (for 10 mL) depending on the percentage of gel required. (sigmaaldrich.com)
• Pour the gel solution in the plates assembled with spacers. (sigmaaldrich.com)
• Add the 5% stacking gel solution until it overflows. (sigmaaldrich.com)
• After the electrophoresis, place the gel in a plastic tray containing gel fix solution. (sigmaaldrich.com)
• Remove the gel fix solution and add Coomassie solution. (sigmaaldrich.com)
• Add destain solution to the gel. (sigmaaldrich.com)
• After destaining, the gels may be stored in gel storage solution and photographed as required. (sigmaaldrich.com)
• Remove the fixing solution and wash the gel for 10 min with 30% ethanol solution followed by wash with ultrapure water for 10 min. (sigmaaldrich.com)
• Decant the water and incubate the gel in sensitizer solution for 10 min. (sigmaaldrich.com)
• Remove the sensitizer solution and wash the gel twice with water, each wash lasting for 10 min. (sigmaaldrich.com)
• Decant silver solution and wash the gel in water for 1.5 min. (sigmaaldrich.com)
• Discard the water and immerse the gel in developer solution for 3 to 7 min. (sigmaaldrich.com)
• Remove the developer/stop solution and wash the gel in ultrapure water for 15 min. (sigmaaldrich.com)