• Isoelectric may refer to: Isoelectric point, the pH at which a particular molecule carries no net electrical charge Isoelectric focusing, a technique for separating different molecules by differences in their isoelectric point Isoelectric line representing the absence of electrical activity on an electrocardiogram Look up isoelectric in Wiktionary, the free dictionary. (wikipedia.org)
  • Isoelectric focusing methodology using LKB 2117 Multiphor 11 electrofocusing apparatus was used for electofocusing and identification of Oreochromis niloticus and Tilapia zilli. (ajol.info)
  • Isoelectric focusing and immunoblotting studies revealed oligoclonal IgG bands in the aqueous of 13 of 23 (57%) patients with FHC, most being of the IgG1 subclass. (birmingham.ac.uk)
  • Capillary isoelectric focusing efficiently analyze heterogeneity of protein drugs. (nii.ac.jp)
  • My colleagues and I recently developed a method to couple immunoaffinity capture and separation by isoelectric focusing in a single capillary. (nii.ac.jp)
  • The method allows sample pretreatment, i.e., purification and concentration, in a single capillary, in which subsequent separation by isoelectric focusing is achieved. (nii.ac.jp)
  • In this study, we developed a workflow employing titanium dioxide phospho-enrichment coupled with isobaric labeling by Tandem Mass Tags (TMT) and high-resolution isoelectric focusing (HiRIEF) fractionation to perform in-depth quantitative phosphoproteomics starting with a low sample quantity. (scilifelab.se)
  • The polymorphism of transferrin (Tf) is currently being studied by isoelectric focusing in carrier ampholyte-generated pH gradients, carrier ampholyte-separator pH gradients or in immobilized pH gradients. (unicatt.it)
  • Isoelectric focusing in carrier ampholyte-separator pH gradients cannot be recommended as a standard typing procedure because the results strongly depend on the batch of carrier ampholytes. (unicatt.it)
  • Tf subtype resolution was only achieved by using isoelectric focusing in immobilized pH gradients with pH slopes reliably reproducible from one experiment to another. (unicatt.it)
  • Dive into the research topics of 'Membrane proteome analysis of microdissected ovarian tumor tissues using capillary isoelectric focusing/reversed-phase liquid chromatography-tandem MS'. Together they form a unique fingerprint. (elsevierpure.com)
  • Isoelectric focusing (IEF) was used to separate at least five major isozymes of AChE. (cdc.gov)
  • The method consisted of isoelectric focusing (IEF) of dog serum, followed by immunoblotting. (lu.se)
  • Carbonic Anhydrase I from human erythrocytes has been used as a pI (isoelectric point) marker in two-dimensional gel electrophoresis. (sigmaaldrich.com)
  • Carbonic Anhydrase I from human erythrocytes is a protein which is used as a standard pI (isoelectric point) marker for isoelectric focussing experiments. (sigmaaldrich.com)
  • Isoelectric point-based fractionation by HiRIEF coupled to LC-MS allows for in-depth quantitative analysis of the phosphoproteome. (scilifelab.se)
  • Proteins are separated in the first dimension based on their isoelectric point, and then in the second dimension by molecular weight. (learner.org)
  • Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
  • This was symbolically termed torsade de pointes, or "twisting of the point" about the isoelectric axis, because it reminded the authors of the torsade de pointes movement in ballet. (medscape.com)
  • Missense mutation, altered isoelectric point. (yale.edu)
  • Inactivation of the catalytic activity with organophosphate pesticides did not change the isoelectric point of the isozymes, and therefore separation of native from organophosphate inhibited AChE did not occur. (cdc.gov)
  • In the second type of C7 deficiency, the quantity of C7 is diminished and the protein exhibits an altered isoelectric point. (lu.se)
  • Isoelectronicity, similarity of valence electrons and structure of different molecules This disambiguation page lists articles associated with the title Isoelectric. (wikipedia.org)
  • HiRIEF fractions with the most acidic isoelectric points are enriched in multiply phosphorylated peptides, which represent 18% of all the phospho-peptides detected in the pH range 2.5-3.7. (scilifelab.se)
  • Seven kinds of enzymes with various isoelectric points (pI) were immobilized on the Fe2O3/ HA composite particles in buffered solution adjusted at pH = 7.40 or pH = 10.0, 36.5 °C. Effects of the enzyme pI and the solution pH on the immobilization were investigated. (elsevierpure.com)
  • Then sonication (200 kHz or 28 kHz) was applied to adjust pH to the isoelectric points and separated particles from solutions by still-standing and spontaneous precipitation. (nih.gov)
  • The sonication of green tuff and andesite suspensions at 200 kHz changed the pH to the isoelectric zone despite the pH buffering effect of eluted alkali earth metals, and successfully precipitated the particles. (nih.gov)
  • On the contrary, the sonication of these suspensions at 28 kHz failed to adjust pH to the isoelectric zone, and the particles did not precipitate. (nih.gov)
  • The third simple way of These limits are even more relevant assessing the ventricular axis consists of the when considering that the diagnosis of location of the most isoelectric limb lead1. (bvsalud.org)
  • At first, the relationship was measured between zeta-potential and pH in these suspensions to find pH levels correspondent to the isoelectric points. (nih.gov)
  • Torsade de pointes is an uncommon and distinctive form of polymorphic ventricular tachycardia (VT) characterized by a gradual change in the amplitude and twisting of the QRS complexes around the isoelectric line (see the image below). (medscape.com)
  • Aunque existen propuestas con algoritmos que utilizan otras search, data collection, data or derivaciones, se exploró un método alternativo con D1 y D3 basados en la fórmula algebraica de la tangente inversa y software management, data método matemático para el cálculo exacto del eje cardíaco. (bvsalud.org)
  • Otto not are an British download handbook of isoelectric focusing and proteomics 2005 of what Description jeweils somatically Then. (erzebet.com.ar)
  • The Regent Taktse Shabdrung and little songs n't was a download handbook of isoelectric focusing and proteomics 2005 to the clinical political pp. that they was Kelsang Gyatso as the Dalai Lama. (erzebet.com.ar)
  • Isoelectric focusing nonporous RP HPLC: a two-dimensional liquid-phase separation method for mapping of cellular proteins with identification using MALDI-TOF mass spectrometry. (nih.gov)
  • The proteins are separated by pI using isoelectric focusing in the first dimension and by hydrophobicity using nonporous reversed-phase HPLC in the second dimension (IEF-NP RP HPLC). (nih.gov)
  • Capillary isoelectric focusing together with antibody-based detection can resolve and identify proteins and their isoforms with modest sample input. (scilifelab.se)
  • Proteins are separated in the first dimension based on their isoelectric point, and then in the second dimension by molecular weight. (learner.org)
  • Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation. (scilifelab.se)
  • Here we applied these DNA-assisted RCA techniques in capillary isoelectric focusing to resolve endogenous signaling transducers and isoforms along vascular endothelial growth factor (VEGF) signaling pathways at concentrations too low to be detected in standard assays. (scilifelab.se)
  • 17. Tissue proteomics using capillary isoelectric focusing-based multidimensional separations. (nih.gov)
  • Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. (yeastgenome.org)
  • The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. (letstalkacademy.com)
  • A proteomics platform combining depletion, multi-lectin affinity chromatography (M-LAC), and isoelectric focusing to study the breast cancer proteome. (nih.gov)
  • The purpose of this study is to restore and characterize fish-protein-isolates (FPI) from the by-products of the sardine canning industry through the application of the isoelectric method of precipitation-solubilization (pH shift) method. (unair.ac.id)
  • The research suggestion that is isoelectric precipitation- solubilization method can be applied to produce new values on the by-products of the fishing industry but by adding antibacterial substances as needed. (unair.ac.id)
  • The collected glycoproteomes were then subjected to isoelectric focusing (IEF) separation by a digital ProteomeChip (dPC), followed by in-gel digestion and LC-MS analysis using an Orbitrap mass spectrometer. (nih.gov)
  • Drops are manipulated discretely on a superhydrophobic surface, subjected to low voltages for isoelectric focusing, and split-resulting in a preparative separation. (elsevierpure.com)
  • The use of analytical isoelectric focusing for detection and identification of beta-lactamases. (nih.gov)
  • Detection of isoforms of recombinant human erythropoietin by various plant lectins after isoelectric focusing. (tuwien.at)
  • Chromatofocusing purification of CD1b-antigen complexes and their analysis by isoelectric focusing. (illumina.com)
  • Important discrepancies in isoelectric points (pI) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). (nih.gov)
  • When the IgG preparation is subjected to isoelectric focusing, it is found to contain four major species with p I values ranging from 6 to 7. (elsevierpure.com)
  • The researchers found that isoelectric events occurred in 63% of patients (95% confidence interval of 49% - 76%), representing 0% - 2.2% of total anesthetic time. (masimo.com)
  • A pediatric electroencephalographer compared the EEG recordings for 9 of the 10 patients monitored with both SedLine and 10-channel EEG and found that isoelectric events observed with SedLine were also observed across all channels with 10-channel EEG, noting "The Matlab program identified 47 isoelectric events from the SedLine EEG, and all events were confirmed by the electroencephalographer. (masimo.com)
  • While the isolated light chain is relatively homogeneous, the heavy chain exhibits a pattern of isoelectric focusing bands similar to that of the intact immunoglobulin. (elsevierpure.com)
  • A novel approach to molecular separations is investigated using a technique termed droplet-based isoelectric focusing. (elsevierpure.com)
  • Dr. Yuan commented, "We were surprised by how prevalent isoelectric EEG occurred in healthy infants and young children undergoing routine general anesthesia, regardless of anesthetic technique. (masimo.com)
  • 24, 2019-- Masimo (NASDAQ: MASI) announced today that in a study recently published in Anesthesia & Analgesia , researchers investigated the incidence of isoelectric electroencephalogram (EEG) events in infants and young children undergoing scheduled surgery requiring general anesthesia. (masimo.com)
  • The surface charge of amphibole minerals in potable water was negative and, in common with most other silicate minerals, the isoelectric point of amphibole asbestos fibers is in the ph range 4 to 6. (cdc.gov)