MalateDehydrogenaseEnzymeSuccinate and glyoxylateEnzymesMethylisocitrateMycobacteriumPhosphoenolpyruvateBiosynthesisMetaboliteCleavageProteinsMoleculeAminoMechanismProcessMalate synthaseInhibitionCitrate lyasePhosphorylmutaseGlyoxylate bypassMycobacteriumMetabolismMycobacteriaTuberculosisDeletionCandidaSuccinateSynthetaseYeastCatalyzes
Malate5
- However, these results have been underestimated because genes for the two key enzymes of the cycle, isocitrate lyase (ICL) and malate synthase (MS), are not annotated in Leishmania genomes. (degruyter.com)
- Isocitrate lyase and malate synthase are specific enzymes of the glyoxylate cycle, used here as glyoxysomal markers. (bioone.org)
- Oxalacetate is the main OAH substrate, but the enzyme also acts as a lyase of (2R,3S)-dimethyl malate with approximately 1000-fold lower efficacy. (rcsb.org)
- the enzymes isocitrate lyase and malate synthase divert it away into biosynthetic processes in the bacterium. (epfl.ch)
- The glyoxylate shunt consists of 2 enzymes, isocitrate lyase (AceA) and malate synthase (AceB). (upb.ro)
Dehydrogenase3
- Activity of purified NAD-specific isocitrate dehydrogenase at modulator and substrate concentrations approximating conditions in mitochondria. (wikipathways.org)
- Three mutants possessing transposons in genes of an acyl-CoA ligase, an acyl-CoA dehydrogenase and an isocitrate lyase display a deficient ability in growing in azelaic acid. (iucc.ac.il)
- Isocitrate dehydrogenase mutations are rare in pheochromocytomas and paragangliomas. (cdc.gov)
Enzyme6
- Isocitrate lyase (EC 4.1.3.1) is an enzyme that catalyzes the conversion of isocitrate to succinate and glyoxylate. (wikipedia.org)
- Isocitrate Lyase from Aspergillus Nidulans: Crystallization and X-ray Analysis of a Glyoxylate Cycle Enzyme. (rcsb.org)
- Comparison between the OAH structure and that of the closely related enzyme, 2,3-dimethylmalate lyase, suggests potential determinants of substrate preference. (rcsb.org)
- Isocitrate lyase (ICL), a signature enzyme of the glyoxylate cycle, is required for metabolism of non-fermentable carbon compounds like acetate or ethanol, and virulence in bacteria and fungi. (slu.se)
- Lyases are a type of enzyme that catalyzes the breakdown of various chemical bonds without the use of water. (pediaa.com)
- Hydrolases are a type of enzyme that use water to break chemical bonds, while lyases are a type of enzyme that catalyzes the breakdown of various chemical bonds without the use of water. (pediaa.com)
Succinate and glyoxylate1
- Isocitrate lyase catalyses the first committed step of the carbon-conserving glyoxylate bypass, the Mg(2+)-dependent reversible cleavage of isocitrate into succinate and glyoxylate. (rcsb.org)
Enzymes2
- There are many different types of enzymes, and hydrolases and lyases are two of them. (pediaa.com)
- Hydrolases and lyases are two types of enzymes. (pediaa.com)
Methylisocitrate1
- Isocitrate lyase InterPro: IPR006254 Methylisocitrate lyase InterPro: IPR012695 Carboxyvinyl-carboxyphosphonate phosphorylmutase InterPro: IPR012697 Beeching JR (1989). (wikipedia.org)
Mycobacterium1
- The results showed that the mycobacterium decides where to direct isocitrate by using a mechanism that is not like a simple on-off switch. (epfl.ch)
Phosphoenolpyruvate1
- Oxalacetate acetylhydrolase (OAH), a member of the phosphoenolpyruvate mutase/isocitrate lyase superfamily, catalyzes the hydrolysis of oxalacetate to oxalic acid and acetate. (rcsb.org)
Biosynthesis2
Metabolite1
- Itaconate, a mammalian antimicrobial metabolite has shown excellent efficiency against isocitrate lyase that causes Tuberculosis. (biosolveit.de)
Cleavage2
- The main difference between hydrolases and lyases is that hydrolases catalyze the hydrolysis of chemical bonds using water molecules, whereas lyases catalyze the cleavage of chemical bonds without the use of water molecules. (pediaa.com)
- Hydrolases catalyze the hydrolysis of chemical bonds using water molecules, whereas lyases catalyze the cleavage of chemical bonds without the use of water molecules. (pediaa.com)
Proteins1
- Isocitrate lyase family is a family of evolutionarily related proteins. (wikipedia.org)
Molecule1
- The molecule in question is called isocitrate, which, once produced, can go in two directions: continue the energy production cycle or be taken away to synthesize other parts of the bacterium. (epfl.ch)
Amino1
- They are the lyases that remove carboxyl groups from amino acids and other organic acids. (pediaa.com)
Mechanism1
- Similarly, the mechanism that controls how isocitrate is used responds to negative feedback, and so it dampens 'noise' to maintain optimal levels. (epfl.ch)
Process1
- Lyases cleave bonds and form new ones through the process of elimination. (pediaa.com)
Malate synthase3
- Enzyme assays validated the increase in isocitrate lyase and malate synthase activities. (nih.gov)
- 2010) did not find any simultaneous positive matches for the glyoxylate cycle key enzymes: isocitrate lyase and malate synthase (with the exception of previous mentioned species Hfx. (casr-signal.com)
- volcanii isocitrate lyase and malate synthase showed that GSI-IX nmr these enzymes are present also in Haladaptatus paucihalophilus strain DX253. (casr-signal.com)
Inhibition4
- 4. Salicylanilide derivatives block Mycobacterium tuberculosis through inhibition of isocitrate lyase and methionine aminopeptidase. (nih.gov)
- 3. Wei J, Leit S, Kuai J, Therrien E, Rafi S, Harwood Jr HJ, DeLaBarre B, Tong L. (2019) An allosteric mechanism for potent inhibition of human ATP-citrate lyase. (guidetopharmacology.org)
- It also substantiated the inhibition of isocitrate lyases by 3-nitropropionate. (nih.gov)
- In addition, competitive inhibition of isocitrate lyases along with a reduction in the inactivation of isocitrate dehydrogenases appears to be a feasible strategy for targeting persistent mycobacteria. (nih.gov)
Citrate lyase2
- ATP-citrate lyase (ACLY) converts cytosolic citrate into acetyl-CoA which is then utilised for lipogenesis. (guidetopharmacology.org)
- 2007) 2-hydroxy-N-arylbenzenesulfonamides as ATP-citrate lyase inhibitors. (guidetopharmacology.org)
Phosphorylmutase1
- Isocitrate lyase InterPro: IPR006254 Methylisocitrate lyase InterPro: IPR012695 Carboxyvinyl-carboxyphosphonate phosphorylmutase InterPro: IPR012697 Beeching JR (1989). (wikipedia.org)
Glyoxylate bypass1
- Overexpression of isocitrate lyase-glyoxylate bypass influence on metabolism in Aspergillus niger. (kimosys.org)
Mycobacterium2
- Previous studies on pathogenic microorganisms, i.e., the bacterium Mycobacterium tuberculosis and the yeast Candida albicans, have revealed an essential role for isocitrate lyase in pathogenicity. (leibniz-hki.de)
- 3. Salicylanilide pyrazinoates inhibit in vitro multidrug-resistant Mycobacterium tuberculosis strains, atypical mycobacteria and isocitrate lyase. (nih.gov)
Metabolism3
- Aspergillus fumigatus does not require fatty acid metabolism via isocitrate lyase for development of invasive aspergillosis. (leibniz-hki.de)
- Schöbel F, Ibrahim-Granet O, Avé P, Latgé JP, Brakhage AA, Brock M (2007) Aspergillus fumigatus does not require fatty acid metabolism via isocitrate lyase for development of invasive aspergillosis. (leibniz-hki.de)
- Elevated sphingosine-1-phosphate lyase leads to increased metabolism and reduced survival in adrenocortical carcinoma. (cdc.gov)
Mycobacteria1
- Substituted N-phenylitaconamides as inhibitors of mycobacteria and mycobacterial isocitrate lyase. (nih.gov)
Tuberculosis3
- M. tuberculosis isocitrate lyase ( Mt ICL), which is absent in host cells, is a key rate-limiting enzyme of the glyoxylic acid cycle and is essential for the survival of dormant M. tuberculosis . (nih.gov)
- Another project will target isocitrate lyase, which is known to be important for M. tuberculosis survival. (bioedonline.org)
- The M. tuberculosis model reproduced the observation that deletion of one ofthe two isocitrate lyase genes has little effect on bacterial growth in macrophages, but deletion of both genes leads to the elimination of the bacilli from the lungs. (nih.gov)
Deletion2
- In this study, we generated an isocitrate lyase deletion strain to test whether this strain shows attenuation in virulence. (leibniz-hki.de)
- In a murine model of invasive aspergillosis, the wild-type strain, an isocitrate lyase deletion strain, and a complemented mutant strain were similarly effective in killing mice. (leibniz-hki.de)
Candida1
- Therefore, an antifungal drug inhibiting fungal isocitrate lyases, postulated to act against Candida infections, is assumed to be ineffective against A. fumigatus. (leibniz-hki.de)
Succinate2
- Isocitrate lyase (EC 4.1.3.1) is an enzyme that catalyzes the conversion of isocitrate to succinate and glyoxylate. (wikipedia.org)
- It catalyzes the conversion of isocitrate to succinate and glyoxylate. (bvsalud.org)
Synthetase1
- 16211) para-aminobenzoate synthetase/4-amino-4-deoxychorismate lyase pabBC BBZA01000002 CDS ARMA_0015 192. (go.jp)
Yeast1
- Yeast Isocitrate Dehydrog. (nih.gov)