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  • donor islets
  • To this aim, gene transfer to isolated donor islets ex vivo is one method that has shown promise. (hindawi.com)
  • These genes, indoleamine 2,3-dioxygenase (IDO), manganese superoxide dismutase (MnSOD), and interleukin (IL)-1 receptor antagonist protein (IRAP), were transferred to isolated NOD donor islets ex vivo then transplanted to NOD scid recipients and evaluated in vivo after diabetogenic T-cell challenge. (hindawi.com)
  • In 2000, Dr. James Shapiro and colleagues published a report describing seven consecutive patients who achieved euglycemia following islet transplantation using a steroid-free protocol and large numbers of donor islets, since referred to as the Edmonton protocol. (wikipedia.org)
  • autoimmune
  • Subsequent studies using NOD recipient mice as a model for IDDM showed that recurrent autoimmune disease was seen in foetal islet isografts but rapid rejection of allografts and foetal pig xenografts also occurred. (bl.uk)
  • gene
  • Selective modulation of plasma lipid or glucose levels in ZDF rats has demonstrated that chronic hyperglycemia, rather than hyperlipidemia, is a determining factor for TG accumulation and decreased insulin gene expression in islets ( 4 ). (diabetesjournals.org)
  • 1 day) culture of normal islets in the presence of supraphysiological concentrations of FAs decreases insulin content and insulin gene expression only in the presence of elevated glucose levels, consistent with the notion that chronic effects of FAs on β-cell function are glucose-dependent. (diabetesjournals.org)
  • We also showed that the decrease in insulin gene expression was associated with a glucose-dependent increase in islet TG content ( 12 ), although we have not provided any evidence for a causal relationship. (diabetesjournals.org)
  • Knockout mice for the CSF-1 gene cause islets to be devoid of resident macrophages. (wikipedia.org)
  • glucose
  • In vitro, we have previously shown that deleterious effects of prolonged exposure of isolated islets to FAs were dependent on the presence of elevated glucose concentration. (diabetesjournals.org)
  • High-fat feeding did not affect triglyceride (TG) content or the rate of glucose oxidation in islets. (diabetesjournals.org)
  • Indeed, several other potential mechanisms have been proposed, including changes in glucose oxidation ( 7 ) and, more recently, modulation of expression of uncoupling protein (UCP)-2 in islets ( 13 , 14 ). (diabetesjournals.org)
  • To elucidate the mechanisms whereby high-fat diet impairs β-cell function in GK rats, we ascertained whether accumulation of TG, changes in glucose oxidation, or modulation of UCP-2 levels in islets are involved. (diabetesjournals.org)
  • Once transplanted, the islets begin to produce insulin, actively regulating the level of glucose in the blood. (wikipedia.org)
  • Yet despite continued procedural improvements, only about 10% of islet recipients in the late 1990s achieved euglycemia (normal blood glucose). (wikipedia.org)
  • The goal of islet transplantation is to infuse enough islets to control the blood glucose level removing the need for insulin injections. (wikipedia.org)
  • Recipient
  • But a transplant recipient will need to take immunosuppressive drugs that stop the immune system from rejecting the transplanted islets. (wikipedia.org)