Islet mitogen activated protein kinase 8. Medical search. Frequent questions

In recent years, the MAPK family has been found to be an important signal-regulating enzyme between cell surface receptors and determinants of gene expression ( 8 ). (spandidos-publications.com)
Insulin signaling affects multiple downstream pathways including mitogen-activated protein kinase (MAPK), which controls growth, sterol regulatory element-binding protein 1 (SREBP-1), which stimulates the synthesis of lipid and cholesterol as well as the family of Forkhead (FOXO) transcription factors regulating metabolism and autophagy [ 9 ] [ 10 ] . (encyclopedia.pub)
Calycosin promotes angiogenesis involving estrogen receptor and mitogen-activated protein kinase (MAPK) signaling pathway in zebrafish and HUVEC. (uchicago.edu)
EPCs were cultured and treated with chemerin together with the specific p38 mitogen-activated protein kinase (MAPK) inhibitor SB 203580 in a time- and dose-dependent manner. (biomedcentral.com)

The protein encoded by this gene is closely related to MAPK8IP1/IB1/JIP-1, a scaffold protein that is involved in the c-Jun amino-terminal kinase signaling pathway. (wikipedia.org)
This gene encodes a scaffold protein that is thought to be involved in the regulation of the c-Jun amino-terminal kinase signaling pathway. (nih.gov)

Immunologic and confocal microscopic evidence also suggested a transient but significant stimulation of tyrosine phosphorylation of Cav-1 in β-cells briefly (for 15 min) exposed to IL-1β that was markedly attenuated by three structurally distinct inhibitors of protein tyrosine phosphorylation. (diabetesjournals.org)
Free circulating insulin activates insulin receptors on the surface of target cells eliciting a signaling cascade initiated by the activation of insulin receptor substrates (IRS 1-4) followed by phosphorylation of phosphoinositide 3-kinase (PI3K), which manages metabolic response including PDK1 and Akt stimulation by phosphorylation. (encyclopedia.pub)
Furthermore, midazolam suppressed phosphorylation of p38 mitogen-activated protein kinase and also inhibited lipopolysaccharide-induced superoxide production in macrophages. (asahq.org)
Davis P.J., Shih A., Lin H.Y., Martino L.J. & Davis F.B., Thyroxine promotes association of mitogen-activated protein kinase and nuclear thyroid hormone receptor (TR) and causes serine phosphorylation of TR. (biologie-journal.org)

Two signaling pathways arising from glucose metabolism converge to activate NFAT-mediated insulin gene transcription. (diabetesjournals.org)
Akt pathways have effects on apoptosis, protein synthesis, metabolism and cell cycle. (springer.com)
AMPK pathways inhibit biosynthetic pathways with energy consumption, such as protein, fatty acid and glycogen synthesis. (springer.com)
NF-κB pathways can be mainly activated by two pathways. (springer.com)
Members of the family of nuclear receptors, the three peroxisome proliferator-activated receptors (PPARs), PPARα, PPARβ/δ, and PPARγ take part in the modulation of these pathways. (encyclopedia.pub)
Two powerful tumour suppressor pathways, controlled by the p53 and retinoblastoma (pRB) proteins, are important for establishing and maintaining the senescence growth arrest. (nature.com)

Leptin and adiponectin can augment the oxidation of fatty acid in liver by activating the nuclear receptor super-family of transcription factors, namely peroxisome proliferator-activated receptor (PPAR)-α. (wjgnet.com)
Comparison of vascular relaxation, lipolysis and glucose uptake by peroxisome proliferator-activated receptor-gamma activation in +db/+m and +db/+db mice. (uchicago.edu)

Summary Human being islet-engrafted immunodeficient mice treated with alogliptin display improved human being insulin secretion and beta cell proliferation compared to control mice engrafted with the same donor islets. (healthweblognews.info)

Zinc has "mimetic" activity where it is involved in a range of functions including insulin receptor signal transduction, insulin storage, secretion and tissues/organelle distribution, and inhibition of protein tyrosine phosphatases [ 5 - 8 ]. (hindawi.com)
With the emergence of pan-cancer tyrosine receptor kinase inhibitors, proper diagnosis of these tumors can help determine appropriate treatments and improve their outcomes. (bvsalud.org)

Insulin implants were eliminated upon transplant of 2000 human being islet equivalents (IEQs). (healthweblognews.info)

Glucose and GLP-1 also synergistically activated NFAT (nuclear factor of activated T-cells)-mediated transcription from a minimal promoter construct containing tandem NFAT consensus sequences. (diabetesjournals.org)
We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)

Differential roles of JIP scaffold proteins in the modulation of amyloid precursor protein metabolism. (nih.gov)

This protein is expressed in brain and pancreatic cells. (wikipedia.org)

The response to GLP-1 was mimicked by forskolin and largely inhibited by the protein kinase A (PKA) inhibitors, H89 and myristoylated PKI(14-22) amide, indicating partial mediation via a cAMP/PKA pathway. (diabetesjournals.org)

Her research group (funded by the BBSRC, The Wellcome Trust and the British Heart Foundation) is investigating the molecular mechanisms important for regulating pro- and anti-inflammatory gene expression and downstream cellular functions in response to a range of external stimuli, including ligands for G protein-coupled receptors and growth factor receptors, and exogenous lipoproteins. (rvc.ac.uk)
The current studies provide novel insights into the pharmacology of GPR40 and indicate that G protein-coupled receptors which respond to fatty acids, and potentially to other lipid ligands, can be occupied by endogenous agonists before assay and that this may mask the pharmacology of the receptor and may be mistaken for high levels of constitutive activity. (aspetjournals.org)

3 Activated macrophages contribute to inflammatory responses and can produce and release several cytokines into the general circulation to exert systemic effects that occur during sepsis. (asahq.org)
6 Cyclooxygenase 2 (COX-2) or inducible nitric oxide synthase (iNOS) is induced in macrophages by proinflammatory stimuli, including mitogens, cytokines, and bacterial lipopolysaccharide. (asahq.org)
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. (rndsystems.com)
TLRs are involved in the pathogenesis of inflammation because they induce inflammatory cytokines and other endogenous molecules to defend against pathogenic microbial infection [ 8 , 9 ]. (researchsquare.com)
Regardless of the need for Th2 cells and their connected cytokines in the pathogenesis of sensitive respiratory disease, research of antigens regarded as causes of T-cell reactions have up to now been mostly limited by those recognized to bind IgE antibodies (7, 8) and induce IgE-mediated instant hypersensitivity reactions (9). (enmd-2076.com)

Human being islet transplantation Human being islets were from the Integrated Islet Distribution System under protocols authorized by the Institutional Review Table of the University or college of Massachusetts Medical School. (healthweblognews.info)

3 contains a cell surface receptor site for thyroid hormone that is linked to activation of mitogen-activated protein kinase and induction of angiogenesis. (biologie-journal.org)
Chemerin is an adipocytokine involved in the recruitment of immune cells [ 11 ] and serves as a bridge between various immune cells from the initial stage of inflammatory diseases through the development process [ 12 ], and its receptor CMKLR1 (also named ChemR23 in humans) is a specific functional member of the G protein receptor family. (biomedcentral.com)
adhesion G protein-coupled receptor E5. (gsea-msigdb.org)

It has been shown to interact with, and regulate the activity of MAPK8/JNK1, and MAP2K7/MKK7 kinases. (wikipedia.org)
Moreover, Akt activates mTOR, which facilitates anabolic processes, while mTORC2 feeds back to regulate Akt [ 8 ] . (encyclopedia.pub)
MiRNAs are small noncoding, double-stranded RNA molecules that can regulate protein expression at the post-transcriptional level by base pairing to the 3' untranslated region (UTR) of their target genes based on complete or incomplete sequence complementarity [ 4 , 5 ]. (biomedcentral.com)

In the present study, we examined potential contributory roles of membrane-associated, cholesterol-enriched lipid rafts/caveolae and their constituent proteins (e.g., caveolin-1 [Cav-1]) as potential sites for IL-1β−induced nitric oxide (NO) release in the isolated β-cell. (diabetesjournals.org)
Novel variant pathway in the synthesis of protein- and lipid-1inked, complex-type oligosaccharides. (or.jp)
Compared with the control group and the inhibitor group, ApoE−/− mice treated with chemerin protein had more serious arterial stenosis, higher lipid contents in plaques and decreased collagen. (biomedcentral.com)

The opposite effects of LS(atheroprotective) and OS(atherogenic) are likely to be determined by differential expression of genes and proteins. (e-dmj.org)
The protein level of key genes was detected using Western blot. (bvsalud.org)

Specificities of GM2 activator protein and GM2A protein. (or.jp)
Activator of protein kinase A (cyclic AMP agonist). (biolog.de)

VEGF is purified from bovine pituitary follicular cells in 1959 and is a vascular endothelial cell-specific mitogen that promotes the growth of vascular endothelial cells. (spandidos-publications.com)

Glucose metabolism activates this pathway by means of increasing [Ca 2+ ] i via L-type voltage-dependent calcium channels (VDCCs) by affecting the electrical activity of the cell. (diabetesjournals.org)
In addition, there is evidence that fatty acids interfere with very early stages of Glucose Transporter Type 4 (GLUT4) and hexokinase II activity [8]. (heraldopenaccess.us)

The results demonstrated that Vitexin pretreatment significantly reduced neuronal apoptosis, and inhibited caspase‑3 activity, apoptosis regulator BAX protein expression and malondialdehyde levels in sevoflurane‑induced newborn rats. (spandidos-publications.com)
Furthermore, it was revealed that treatment with vitexin induced hypoxia inducible factor 1α subunit (HIF‑1α) and vascular endothelial growth factor (VEGF) protein expression, and suppressed phosphorylated‑p38 MAP kinase (p38) protein expression in sevoflurane‑induced newborn rat. (spandidos-publications.com)
These results suggest that midazolam has an antiinflammatory action by inhibiting inducible nitric oxide synthase and cyclooxygenase-2 expression, possibly through suppression of NF-kappaB and p38 mitogen-activated protein kinase activation. (asahq.org)
A three-dimensional culture system ( 2 ) is more reliable than a two-dimensional culture system regarding cell growth, cell metabolism, gene expression, extracellular matrix proteins, and cell morphology ( 3 ). (brieflands.com)
Gremlich S., Bonny C., Waeber G. & Thorens B ., Fatty acids decrease IDX-1 expression in rat pancreatic islets and reduce GLUT2, glucokinase, insulin, and somatostatine levels. (biologie-journal.org)
miR-218-5p expression significantly correlated with IL-6, IL-8, TNF-α, and IFN-γ expression. (researchsquare.com)
We found that loss of HPK1 protein expression correlated significantly with the progression of pancreatic intraepithelial neoplasias ( P = 0.001) and development of invasive PDA. (aacrjournals.org)
Treatment of Panc-1 cells with a proteasome inhibitor, MG132, increased the HPK1 protein levels in a dose-dependent manner, suggesting that alteration in proteasome activity contributes to the loss of HPK1 protein expression in pancreatic cancer. (aacrjournals.org)
After MG132 withdrawal, wild-type HPK1 protein expression was markedly decreased within 24 hours, but kinase-dead HPK1 mutant protein expression was sustained for up to 96 hours. (aacrjournals.org)
Furthermore, restoring wild-type HPK1 protein in PDA cells led to the increase in p21 and p27 protein expression and cell cycle arrest. (aacrjournals.org)
Exposing endothelial cells to OS increased BMP4 protein expression while LS decreased it. (e-dmj.org)
Neuronal injury in the hippocampal dentate gyrus and ribosomal protein L6 (RPL6) expression in the brain tissues were detected using H&E staining, Nissl staining, and Western blot. (bvsalud.org)
In cells lacking expression of Ca 2+ -mobilizing G proteins, coexpression of human GPR40 and Gα q allowed medium- and long-chain fatty acids to elevate intracellular [Ca 2+ ]. (aspetjournals.org)

Thus, the proteins that transport zinc likely facilitate cell signaling processes that contribute to glycemic control in peripheral tissues by modulating cytosolic zinc concentrations. (hindawi.com)
The central feature of COPD is inflammation affecting lung parenchyma and peripheral airways which activates inflammatory cell infiltration, including alveolar macrophages and neutrophils, and other cells. (researchsquare.com)

We recently reported that the activation of H-Ras represents one of the signaling steps underlying the interleukin-1β (IL-1β)−mediated metabolic dysfunction of the islet β-cell. (diabetesjournals.org)

C-jun-amino-terminal kinase-interacting protein 2 is a protein or the name of the gene that encodes it. (wikipedia.org)
The gene is also known as Islet-Brain-2 (IB2). (wikipedia.org)
NFAT binds to three distinct NFAT elements within the rat I insulin promoter and activates insulin gene transcription. (diabetesjournals.org)
One pathway results from the direct effect of increased [Ca 2+ ] i , which activates calcineurin, and in turn, it upregulates insulin gene transcription via NFAT. (diabetesjournals.org)

Objective@#To investigate the regulatory relationship of Protein Phosphatase 2 Regulatory Subunit B"Alpha ( PPP2R3A) and hexokinase 1 ( HK1) in glycolysis of hepatocellular carcinoma (HCC). (bvsalud.org)

Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)
Allergic respiratory illnesses are connected with high degrees of IgE antibodies to particular allergenic protein and elevated degrees of eosinophils that infiltrate the prospective tissue (5). (enmd-2076.com)

The actions of the proteins that control the uptake, storage, and distribution of zinc, the zinc transporters, are under intense investigation due to their emerging role in type 2 diabetes. (hindawi.com)
After diabetes was confirmed, mice were given insulin implants (LinShin Canada, Inc, Toronto, ON, Canada) until human being islets were available for transplant. (healthweblognews.info)
The 30 mg/kg/day time dosage is definitely mid-range between doses (15 and 45 mg/kg) that have previously been shown to be effective in repairing beta cell mass and islet function in two different mouse models of diabetes.19,20 Daily treatments were continued until graft removal at 32C39 days post-transplant. (healthweblognews.info)

Hematopoietic progenitor kinase 1 (HPK1) regulates stress responses, proliferation, and apoptosis in hematopoietic cells. (aacrjournals.org)

More than 90% of extracellular miRNAs are vesicle-free, but form a complex with proteins such as Agonaute2 (AGO2) ( 22 , 23 ). (frontiersin.org)

These results demonstrate a mixed unbiased transcriptomic, proteomic, and immunomic strategy identifies a significantly broadened repertoire of proteins antigens targeted by T cells involved with allergy pathogenesis. (enmd-2076.com)

Like the endogenous HPK1, both wild-type HPK1 and its kinase-dead mutant, HPK1-M46, overexpressed in Panc-1 cells, were also targeted by proteasome-mediated degradation. (aacrjournals.org)

tolerance tests were significantly improved by alogliptin treatment for mice transplanted with islets from two of the three human being islet donors. (healthweblognews.info)
Islet-engrafted mice treated with alogliptin also experienced significantly higher plasma levels of human being insulin and C-peptide compared to vehicle settings. (healthweblognews.info)

Percent human being beta cell proliferation in islet grafts from vehicle control (n=6 total, 2 per islet donor) and alogliptin-treated (n=6 total) mice. (healthweblognews.info)
Open in a separate window Figure 5 Insulin+BrdU+ beta cells in human being islet grafts from alogliptin-treated mice. (healthweblognews.info)
Notes: Representative micrographs of human being islets from a single donor (Donor 1) engrafted in (A) vehicle control or (B) alogliptin-treated mice are demonstrated. (healthweblognews.info)
The percentage of insulin+BrdU+ cells in human being islet grafts from alogliptin-treated mice was approximately 10-fold more than from vehicle control mice, consistent with a significant increase in human being beta cell proliferation. (healthweblognews.info)
Immunodeficient mice transplanted with human being islets provide a useful model to interrogate potential therapies to improve human being islet function and survival in vivo. (healthweblognews.info)
The goal of this study was to make use of STZ-induced diabetic NSG mice transplanted with human being pancreatic islets to determine the ability of alogliptin to enhance human being beta cell function and proliferation. (healthweblognews.info)

Here, we describe a multiplexed protein biomarker detection method on the COMET instrument, coined sequential ImmunoFluorescence (seqIF). (bvsalud.org)
Captured proteins are visualized using chemiluminescent detection reagents (Step 3) . (rndsystems.com)
After detection, the array data were quantitated to generate a protein profile (histogram). (rndsystems.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Death Associated Protein Kinase 1 (DAPK1) in Tissue homogenates and other biological fluids. (tuberculosisdrugresearch.org)
Description: A sandwich ELISA kit for detection of Death Associated Protein Kinase 1 from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (tuberculosisdrugresearch.org)
Description: A sandwich quantitative ELISA assay kit for detection of Human Death Associated Protein Kinase 1 (DAPK1) in samples from tissue homogenates, cell lysates or other biological fluids. (tuberculosisdrugresearch.org)

arginine and serine rich protein 1 [So. (gsea-msigdb.org)

Drosha, RNase III, and DGCR8, the RNA-binding protein, further process pri-miRNAs into stem-loop structured precursor miRNAs (pre-miRNAs) of ~70 nt ( 29 ). (frontiersin.org)
After pre-miRNAs are transported to the cytoplasm, RNase III Dicer and TRBP (transactivation-response RNA-binding protein) cleave them into double-stranded miRNA duplexes of ~22 nt ( 30 , 31 ). (frontiersin.org)

Again, these proteins could be expressed in bacteria but were aggregated when analyzed by gel filtration. (trilenio.com)

The syndrome of watery diarrhea, hypokalemia , and achlorhydria (ie, WDHA syndrome) is a rare condition characterized by severe, watery diarrhea resulting from the oversecretion of vasoactive intestinal peptide (VIP) from non-beta pancreatic islet cells. (medscape.com)
Using a macrophage cell line, RAW264.7 cells, the effect of midazolam on proinflammatory mediators and activation of mitogen-activated protein kinase was measured by Western blot. (asahq.org)
Similarly, HPK1 protein was not expressed in any of eight PDA cell lines examined but was expressed in immortalized human pancreatic duct epithelial (HPDE) cells. (aacrjournals.org)
in addition, obesity increases the production of IL-1β, which may have cytotoxic effects on beta islet cells [7]. (heraldopenaccess.us)
The finding of proteins that creates Th2 cells but aren't IgE reactive may permit the advancement EI1 of safer immunotherapeutic strategies. (enmd-2076.com)
Although occasionally it's been demonstrated that T cells can only just or preferentially offer help B cells particular for the same proteins (17, 18), in additional systems this limitation was not the situation (19, 20). (enmd-2076.com)
It had been discovered that two protein that can be found on a single particle could function collectively which T cells particular for one proteins could offer help for B cells particular for the next proteins (20). (enmd-2076.com)
This was also observed when human embryonic kidney (HEK) 293 cells were transfected with a GPR40-Gα q fusion protein. (aspetjournals.org)

Other adipokines, such as leptin and adiponectin and, to a lesser extent, resistin and retinol binding protein-4 are also involved. (wjgnet.com)

Anatomy25

Organisms9

Diseases2

Chemicals and Drugs163

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes38

Disciplines and Occupations1

Information Science3

MAPK4

Signaling pathway2

Phosphorylation4

Pathways6

Peroxisome prolifer2

Insulin Secretion1

Tyrosine2

20001

Nuclear2

Scaffold proteins1

Pancreatic cells1

Inhibitors1

Receptors2

Cytokines5

Transplantation1

Receptor3

Regulate3

Lipid3

Genes2

Activator2

Promotes1

Glucose2

Expression13

Peripheral2

Metabolic1

Gene4

Hexokinase1

Antibodies2

Diabetes3

Apoptosis1

Extracellular1

Pathogenesis1

Endogenous1

Significantly2

Mice6

Detection6

Serine1

MiRNAs2

Bacteria1

Cells8

Leptin1