• Increasingly, complimentary cell culture techniques, which recapitulate the developmental microenvironment, are employed to coax cells to adopt new identities by indirectly regulating transcription factor activity via intracellular signalling pathways. (silverchair.com)
  • Nuclear staining was also present, as was staining of intracellular and extracellular vesicles with ISL1 antibody. (bvsalud.org)
  • The identification of key master regulator transcription factors (which distinguish one body part from another) during embryonic development has been central in developing transdifferentiation protocols. (silverchair.com)
  • CONCLUSIONS: These preliminary results suggest that ISL1 could distinguish a readily available source of putative stem cells in human breast milk. (bvsalud.org)
  • A better understanding of the levers governing transcription factor activity benefits our ability to generate therapeutic cell types at will. (silverchair.com)
  • Both transcription factor-based reprogramming and directed differentiation approaches ultimately exploit transcription factors to influence cellular identity. (silverchair.com)
  • We examine how transdifferentiation protocols are evolving to ever more faithfully recapitulate normal developmental biology using increasingly sophisticated biomimetic techniques and ectopic transcription factor expression. (silverchair.com)
  • OBJECTIVE: ISL1 is a pioneer transcription factor that plays important roles in cell lineage specification and differentiation, by programming the epigenome and recruiting additional regulatory factors. (bvsalud.org)
  • Successful differentiation is guided in large part by epigenetic reprogramming and regulation of critical gene expression patterns. (biomedcentral.com)
  • Here, we explore the evolution of reprogramming and directed differentiation approaches within the context of hepatocyte to β-cell transdifferentiation focussing on how the introduction of new techniques has improved our ability to generate β-cells. (silverchair.com)
  • Here, we discuss how transcription factors, and their unique position as the gatekeepers of cellular identity, are exploited in cell reprogramming protocols by exploring work focusing on one reprogramming paradigm - the transdifferentiation of hepatocytes to pancreatic beta cells (β-cell). (silverchair.com)
  • Cell morphology was examined in the breast milk with the automatic ThinPrep® processor (Hologic® Inc.) in commercial Cytological ThinPrep® solution (Hologic® Inc.), followed by standard immunohistochemical staining of ISL1. (bvsalud.org)
  • RESULTS: ISL1 had a granular diffuse cytoplasmic localization, with varying intensity of staining in both single and grouped cells. (bvsalud.org)
  • LIM-homeodomain component responses LHX2, LHX3, LHX4, LHX9 and ISL1 encode previously then reduced established in a host polymerase SLC17 cytokine of ROBO1, ROBO2, ROBO3 and SLIT2( Wilson et al. (erik-mill.de)
  • One well-established example of transdifferentiation is the conversion of hepatocytes to pancreatic β-cells. (silverchair.com)
  • Positive cells showed a membranous and/or cytoplasmic immunostaining, no reactivity was observed in the nuclear compartment. (bvsalud.org)
  • CD44 expression was always restricted to IVD precursor cells, whereas cartilage precursors were devoid of labelling. (bvsalud.org)
  • Here, we will review the evidence from animal models supporting the role of all major types of liver epithelial cells: hepatocytes, cholangiocytes, and their common progenitor as liver cancer cell-of-origin. (biomedcentral.com)
  • 35. Hepatocyte nuclear factor 4 alpha ligand binding and F domains mediate interaction and transcriptional synergy with the pancreatic islet LIM HD transcription factor Isl1. (nih.gov)
  • The purpose of this review is to summarize and evaluate the evidence supporting the role of hepatocytes, cholangiocytes and progenitor cells as CoO in liver cancer and to highlight possible mechanisms responsible for changing cell identity, a process that may confound prudent determination of CoO in liver cancer (summarized in Fig. 1 and Table 1 ). (biomedcentral.com)
  • The potency of TGFβ is partly based on its ability to perform two parallel molecular functions, i.e. to induce the expression of growth factors, cytokines and chemokines, which sequentially and in a complementary manner help to establish and maintain the EMT, and to mediate signaling crosstalk with other developmental signaling pathways, thus promoting changes in cell differentiation. (mdpi.com)
  • 25. RASSF1A promoter methylation and expression analysis in normal and neoplastic kidney indicates a role in early tumorigenesis. (nih.gov)