Our pathway activator screen yielded a diverse set of hits that promoted nuclear translocation of IRF3 and/or NFkB, but the majority of these compounds did not cause activation of downstream pathways. (harvard.edu)
Interferon production2
In the absence of EGFR-mediated phosphorylation, STING rapidly transits into autophagosomes, and IRF3 activation, interferon production, and antiviral activity are compromised in cell cultures and mice, while autophagic activity is enhanced. (nih.gov)
2. IRF3-binding lncRNA-ISIR strengthens interferon production in viral infection and autoinflammation. (nih.gov)
Phosphorylation and activation2
A 2015 study shows phosphorylation of innate immune adaptor proteins MAVS, STING and TRIF at a conserved pLxIS motif recruits and specifies IRF3phosphorylation and activation by the Serine/threonine-protein kinase TBK1, thereby activating the production of type-I interferons. (wikipedia.org)
Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3phosphorylation and activation by host IKBKE. (joplink.net)
IRF711
IRF3 has been shown to interact with IRF7. (wikipedia.org)
To explore the role of IRF3/IRF7 during inflammatory responses, we investigated the effects of swine IRF3/IRF7 on TLR4 signaling pathway and inflammatory factors expression in porcine kidney epithelial PK15 cell lines. (oncotarget.com)
We successfully constructed eukaryotic vectors PB-IRF3 and PB-IRF7, transfected these vectors into PK15 cells and observed GFP under a fluorescence microscope. (oncotarget.com)
We found that IRF3/IRF7 was efficiently overexpressed in PK15 cells. (oncotarget.com)
Moreover, we evaluated the effects of IRF3/IRF7 on the TLR4 signaling pathway and inflammatory factors by RT-PCR. (oncotarget.com)
We revealed that IRF3/IRF7 enhanced IFNα production, and decreased IL-6 mRNA expression. (oncotarget.com)
This illustrated that IRF3/IRF7 enhanced IFNα production through TLR4/TBK1 signaling pathway and played an anti-inflammatory role, while IRF3/IRF7 decreased IL-6 expression independent of the TBK1 pathway. (oncotarget.com)
These results demonstrated that IRF3/IRF7 regulated the inflammatory response through the TLR4 signaling pathway. (oncotarget.com)
Overexpression of swine IRF3/IRF7 in PK15 cells induced type I interferons production, and attenuated inflammatory responses through TLR4 signaling pathway. (oncotarget.com)
Interferon regulatory factor 3 (IRF3) and IRF7 are closely related IRF members and the major factors for the induction of interferons, a key component in vertebrate innate immunity. (cdc.gov)
N substitution are also found in amphibian IRF3 but not in amphibian IRF7. (cdc.gov)
TBK12
As a response not only represents the first barrier against infec- result, different cellular kinases, including the IRF3 kinas- tion but also provides the appropriate signals required for es TBK1 and IKK, become activated. (cdc.gov)
Residing on the endoplasmic reticulum, STING binds cGAS-generated cGAMP or bacterial CDNs and undergoes trafficking to the Golgi complex in perinuclear space where it recruits TANK-binding kinase 1 (TBK1) and transcription factor interferon regulatory factor 3 (IRF3). (uni-muenchen.de)
Activation2
As a result of the pathway activation IRF3 gets phosphorylated, dimerizes and translocates into the nucleus resulting in type I interferons (IFNs) production. (uni-muenchen.de)
STING was initially thought to be an adaptor protein with its sole role dependent on pattern recognition receptor (PRR) detection of cytosolic double stranded DNA leading to IRF3 driven canonical pathway activation. (lancs.ac.uk)
Protein2
The ancestral protein of IRF3 and 7 seemed to possess the DPHK motif. (cdc.gov)
RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I inter. (invivogen.com)
MAVS2
Aggregated MAVS have been found to activate IRF3 dimerization. (wikipedia.org)
13. MAVS-dependent IRF3/7 bypass of interferon β-induction restricts the response to measles infection in CD150Tg mouse bone marrow-derived dendritic cells. (nih.gov)
Antiviral3
1. Elimination of GPR146-mediated antiviral function through IRF3/HES1-signalling pathway. (nih.gov)
6. KAT8 selectively inhibits antiviral immunity by acetylating IRF3. (nih.gov)
18. c-Cbl-mediated ubiquitination of IRF3 negatively regulates IFN-β production and cellular antiviral response. (nih.gov)
Signaling2
We screened a library of bioactive small molecules for activators and inhibitors of innate immune signaling through IRF3 and NFkB pathways with the goals of advancing pathway understanding and discovering probes for immunology research. (harvard.edu)
8. Role of Herpes Simplex Virus 1 γ34.5 in the Regulation of IRF3 Signaling. (nih.gov)
Innate immune1
IRF3 plays an important role in the innate immune system's response to viral infection. (wikipedia.org)
Regulates1
Structure-activity relationships (SARs) and subsequent chemical proteomics experiments suggested that MAPKAPK5 (PRAK) is a kinase that regulates IRF3 translocation in human macrophages. (harvard.edu)
Cytoplasmic1
IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. (wikipedia.org)
STING1
Here, we report that phosphorylation of a specific tyrosine residue in STING by the epidermal growth factor receptor (EGFR) is required for directing STING to endosomes, where it interacts with its downstream effector IRF3. (nih.gov)
Immune1
Activated IRF3, the subsequent adaptive cellular and humoral immune together with NF-B and AP-1, accumulate in the nucleus, responses to develop. (cdc.gov)
Transcription1
IRF3 is a member of the interferon regulatory transcription factor (IRF) family. (wikipedia.org)
Gene1
41 disease terms (MeSH) has been reported with IRF3 gene. (cdc.gov)
Tissue1
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Interferon Regulatory Factor 3 (IRF3) in Tissue homogenates, cell lysates and other biological fluids. (signetlabs.com)
Type1
The same study identified IRF3 and the type I IFN response as a potential therapeutic target for post-myocardial infarction cardioprotection. (wikipedia.org)
Found1
N substitution in IRF3 DPHK is again found in cetaceans such as whales and dolphins as well as in marsupials. (cdc.gov)
Domain2
IRF3 has been further characterized and shown to contain several functional domains including a nuclear export signal, a DNA-binding domain, a C-terminal IRF association domain and several regulatory phosphorylation sites. (wikipedia.org)
One motif, GASSL, is highly conserved throughout the evolution of IRF3 and 7 and located in the signal response domain. (cdc.gov)