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  • membrane
  • Consistent with these findings, reduced expression of filamin-A, IQGAP1 or RacGAP1 triggered unconstrained membrane protrusion and disrupted directional cell migration on fibrillar extracellular matrices. (biologists.org)
  • siRNA
  • Gene silencing of IQGAP1 by transfection of small interfering RNA (siRNA) with oligofectamine maintained a higher endothelial electrical resistance in HUVECs as compared to transfection of a scrambled siRNA. (cdc.gov)
  • Interaction
  • This interaction is disrupted by the expression of the GTPase CDC42, which is known to bind the C-terminal region of IQGAP1. (biologists.org)
  • Here, we use a combination of biochemical and cell biological approaches to show that the endocytosis of E-cadherin is regulated by the activity state of E-cadherin through the activation of the Rac/Cdc42-IQGAP1 system induced by E-cadherin trans interaction. (rupress.org)
  • capillary
  • METHODOLOGY/PRINCIPAL FINDINGS: Ischemia was induced by left femoral artery ligation, which resulted in increased IQGAP1 expression in Mac3(+) macrophages and CD31(+) capillary-like ECs in ischemic legs. (uic.edu)
  • Mice lacking IQGAP1 exhibited a significant reduction in the post-ischemic neovascularization as evaluated by laser Doppler blood flow, capillary density and α-actin positive arterioles. (uic.edu)
  • angiogenesis
  • IQGAP1 expression is increased in a mouse hindlimb ischemia model of angiogenesis. (ahajournals.org)
  • CONCLUSIONS/SIGNIFICANCE: IQGAP1 plays a key role in post-ischemic neovascularization by regulating, not only, ECs-mediated angiogenesis but also macrophage infiltration as well as ROS production. (uic.edu)
  • Thus, IQGAP1 is a potential therapeutic target for inflammation- and angiogenesis-dependent ischemic cardiovascular diseases. (uic.edu)
  • Furthermore
  • Furthermore, IQGAP1(-/-) mice showed a decrease in macrophage infiltration and ROS production in ischemic muscles, leading to impaired muscle regeneration and increased necrosis and fibrosis. (uic.edu)
  • migration
  • In vitro, IQGAP1(-/-) BM-derived macrophages showed inhibition of migration and adhesion capacity, which may explain the defective macrophage recruitment into the ischemic tissue in IQGAP1(-/-) mice. (uic.edu)
  • adherens junction
  • The objectives of the present study were to determine if IQGAP1 associates with components of the endothelial adherens junction and if it affects endothelial barrier function. (cdc.gov)
  • novel
  • This led to the discovery of a novel multi-vesicular compartment that is surrounded by an outer layer of IQGAP1-associated actin filaments. (rice.edu)
  • Moreover
  • Moreover, thioglycollate-induced peritoneal macrophage recruitment and ROS production were inhibited in IQGAP1(-/-) mice. (uic.edu)
  • Moreover, expression of Wnt target genes caused by depletion of endogenous IQGAP1 could be rescued by expression of wild-type IQGAP1, but not IQGAP1 deleting DVL binding region. (paperity.org)
  • Moreover, the accumulation of IQGAP1 at the antigen contact site depends on F-actin reorganization that is controlled by Rap1 and cofilin-1. (biologists.org)
  • assay
  • LS-F15972 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IQGAP1. (lsbio.com)
  • accumulation
  • In Xenopus embryos, depletion of IQGAP1 reduced Wnt-induced nuclear accumulation of DVL, and expression of Wnt target genes during early embryogenesis. (paperity.org)
  • processes
  • This dissertation details the use of multiple imaging modalities to characterize localized, highly-dynamic IQGAP1-related processes in epithelial MCF-10A cells. (rice.edu)
  • mice
  • When subjected to pressure overload, IQGAP1-null mice initially develop a compensatory hypertrophy indistinguishable from that of wild-type (WT) mice. (biomedsearch.com)
  • expression
  • Endogenous expression of Wnt target genes was reduced by depletion of IQGAP1 during early embryogenesis, but notably not by depletion of other IQGAP family genes. (paperity.org)
  • cells
  • In human umbilical vein endothelial cells (HUVECs), soluble IQGAP1 associated with VE-cadherin and the catenins, b, y, and a, but not N-cadherin. (cdc.gov)
  • BM transplantation revealed that IQGAP1 expressed in both BM-derived cells and tissue resident cells, such as ECs, is required for post-ischemic neovascularization. (uic.edu)