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Chromatography, High Pressure LiquidPressureSpectrometry, Mass, Electrospray IonizationHydrostatic PressureMass SpectrometryChromatography, Thin LayerBlood PressureChromatography, GelChromatography, LiquidAmino Acid SequenceChromatography, AffinityChromatography, Ion ExchangeChromatographyKineticsChromatography, GasMolecular Sequence DataMolecular WeightAtmospheric PressurePeptide FragmentsGas Chromatography-Mass SpectrometryElectrophoresis, Polyacrylamide GelHigh Pressure Neurological SyndromeTrypsinAir IonizationLavandulaAmino AcidsSpectrophotometry, UltravioletSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationArachidonate LipoxygenasesMagnetic Resonance SpectroscopyLipoxygenaseHydrogen-Ion ConcentrationCattleRadioimmunoassayIndicators and ReagentsSpectrometry, Mass, Fast Atom BombardmentHydroxyeicosatetraenoic AcidsSolventsPeptidesSubstrate SpecificityCyanogen BromideBiotransformationRats, Inbred StrainsTime FactorsPeptide MappingCarbohydrate SequenceArachidonic AcidsTandem Mass SpectrometryIsomerismStereoisomerismOligosaccharidesAir PressureSRS-ANeurotensin12-Hydroxy-5,8,10,14-eicosatetraenoic AcidIonic LiquidsMolecular StructureHydrolysisRabbitsBrain ChemistrySwineBinding SitesAffinity LabelsIsoelectric FocusingLiverModels, ChemicalBlood Pressure DeterminationFlame IonizationRecombinant ProteinsMicrosomes, LiverEscherichia coliOxidation-ReductionArachidonic AcidProtein ConformationCell LineTemperatureTritiumBileEndopeptidasesHydroxylationDogsBase SequenceProtein BindingHypertensionMacromolecular SubstancesCarbon RadioisotopesDose-Response Relationship, DrugChemistryPhospholipidsTransducers, PressureLysineCysteineChemical PhenomenaIntracranial PressureIntraocular PressureDNACloning, MolecularKidneyProtein Processing, Post-TranslationalBlood Pressure Monitoring, Ambulatory
Performance liquid chromatographyHPLCSpectrometricDetectionTandem mass spectroIsolated by reversed phaseCapillaryAnalyticalAnalytesSelectiveSpectrometryLipidsReversed-phaseQuantificationMethodJournalAffinityCompoundsRapidColumnRetentionBiomarkersInhibitorsConcentrationsTriacylglycerolsSystemDevelopmentModePrevalenceAnalysisTypeGenerallyPositiveQualityPatientsSimilarBloodExpressionStudy
Performance liquid chromatography12
  • Conifer seed oils from European Larch (Larix decidua), Norway Spruce (Picea abies) and European Silver Fir (Abies alba) have been analyzed by non-aqueous reversed-phase high-performance liquid chromatography (NARP-HPLC) with atmospheric pressure chemical ionisation (APCI)-MS detection. (unboundmedicine.com)
  • The test principle utilizes high performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry (HPLC-APPI-MS/MS) for the quantitative detection of genistein, daidzein, equol, O-desmethylangolensin, enterodiol, and enterolactone. (cdc.gov)
  • In 2003-2004 high performance liquid chromatography electrospry ionization mass spectrometric (LC-ESI-MS/MS) detection (Rybak, M. E. et al. (cdc.gov)
  • 2008) was utilized and in 2005-2006 high performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry (LC-ESI-MS/MS) was utilized. (cdc.gov)
  • Method for determination of acephate, methamidophos, omethoate, dimethoate, ethylenethiourea and propylenethiourea in human urine using high-performance liquid chromatography-atmospheric pressure chemical ionization tandem mass spectrometry. (cdc.gov)
  • The derivatives were analyzed by high-performance liquid chromatography (HPLC) (Kamoda S, Nakano M, Ishikawa R, Suzuki S, Kakehi K. 2005. (edu.au)
  • Rapid and sensitive screening of N-glycans as 9-fluorenylmethyl derivatives by high-performance liquid chromatography: A method which can recover free oligosaccharides after analysis. (edu.au)
  • To characterize the triglycerides in modified soybean oil samples, a technique called high-performance liquid chromatography (HPLC) was used to separate the different triglycerides. (usda.gov)
  • Extracts of plant material or of dyed silk or wool were analyzed by HPLC with photodiode array and mass spectrometric detection using an Agilent 1100 high performance liquid chromatography system consisting of an automatic injector, a gradient pump, a Hewlett-Packard series 1100 photodiode array detector, and an Agilent series 1100 VL on-line atmospheric pressure ionization electrospray ionization mass spectrometer. (mfa.org)
  • The most common form of liquid chromatography in use today is high-performance liquid chromatography (HPLC), which pumps the sample mixture through the column at high pressure. (europeanpharmaceuticalreview.com)
  • A targeted ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was established and validated for the simultaneous determination of 34 amino acids in tissue samples from colorectal cancer (CRC) patients. (sagepub.com)
  • A sensitive and accurate high performance liquid chromatography-mass spectrometric (HPLC-MS) method for the intracellular determination of 14 antiretroviral drugs in peripheral blood mononuclear cells (PBMCs) for HIV+ patients was validated. (unito.it)
HPLC11
  • Various methods such as polarography, piezoelectric crystals, high-pressure liquid chromatography (HPLC) with flame ionization (FID), electrochemical (EC), or ultraviolet (UV) detector, linear sweep voltammetry (LSV), GC with thermal energy analyzer (TEA), FID, or ECD, and thin-layer chromatography (TLC) have been investigated for detecting propylene glycol dinitrate in environmental samples. (cdc.gov)
  • Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). (pseudomonas.com)
  • In order to isolated the active compounds quickly, the strategy of HPLC-ESI-Q-TOF-MS/MS combined with high-speed counter-current chromatography (HSCCC) was implemented. (researchsquare.com)
  • Therefore, the present work is focus on isolating target sesquiterpenes rapidly via the strategy of high-pressure liquid chromatography coupled with electrospray ionization-quadrupole time-of-flight mass spectrometry (HPLC-ESI-Q-TOF-MS/MS) combined with high-speed counter-current chromatography (HSCCC). (researchsquare.com)
  • 1) in the first part of the study, the analytical methods examined were isocratic high pressure liquid chromatography (HPLC) with ultraviolet (UV) detection, capillary gas chromatography (GC) with temperature programming and flame ionisation detection and gas chromatography-mass spectrometry (GC-MS). Twelve NSAIDs in common use in the UK were selected as test compounds for the evaluation of the separating ability, selectivity and sensitivity of detection of the three methods. (gla.ac.uk)
  • Its hydrolytic specificity was established by analyzing the Bacillus subtilis peptidoglycan digestion products by coupling reverse phase-high-pressure liquid chromatography (RP-HPLC) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis, which displayed an N-acetylglucosaminidase activity. (pasteur.fr)
  • Canola oil triacylglycerols from genetically modified canola lines were conclusively identified by reversed-phase HPLC coupled with atmospheric pressure chemical ionization mass spectrometric (APCI-MS) detection. (usda.gov)
  • The LC/APCI-MS identification of canola oil triacylglycerols allowed their quantitation by reversed-phase HPLC coupled with a commercial flame ionization detector (FID). (usda.gov)
  • HPLC is the form of liquid chromatography that is generally used in the pharmaceutical industry, as it can provide the precise results that are required. (europeanpharmaceuticalreview.com)
  • Although expected at first to be used as a complimentary method to gas chromatography, the pharmaceutical industry now almost exclusively uses HPLC as a chromatographic technique. (europeanpharmaceuticalreview.com)
  • 3 HPLC is especially suitable for compounds that are not easily volatilised, thermally unstable and have high molecular weights. (europeanpharmaceuticalreview.com)
Spectrometric3
  • The aim of this study was the evaluation of the first commercially available in-vitro diagnostic (IVD)- mass spectrometric immunosuppressant assay from Chromsystems (MassTox Immunosuppressants ONEMINUTE Test) and the comparison to a routinely used online solid phase extraction liquid chromatography-tandem mass spectrometric assay method for the measurement of cyclosporine A, everolimus, sirolimus, and tacrolimus in patient whole blood samples. (nih.gov)
  • An API 4000 [tandem mass spectrometer (AB SCIEX)] combined with a CTC Pal autosampler (CTC Analytics AG) and a Shimadzu ultra-fast liquid chromatography (UFLC) system were applied for the direct liquid chromatography-tandem mass spectrometric assay analysis using electrospray ionization in positive ion mode. (nih.gov)
  • c) GC-MS : Gas chromatography with mass spectrometric detection was carried out using similar GC conditions to those described above. (gla.ac.uk)
Detection6
  • The primary method of analysis for propylene glycol dinitrate and its metabolites in blood and urine is gas chromatography with electron capture detection (Litchfield 1968). (cdc.gov)
  • The high incidence of these replacements and their detection also in other cancer cell lines imply that the conserved tandem repeat domain of MUC1 is polymorphic with respect to the peptide sequence. (nih.gov)
  • In order to improve the limits of detection (LODs), large volume injection (LVI) using programmable temperature vaporizer (PTV) coupled to gas chromatography-mass spectrometry (GC-MS) was also optimized. (who.int)
  • We use a new method of introduction, called Atmospheric Pressure Chemical Ionization, to spray the triglycerides into the mass spectrometer for detection. (usda.gov)
  • However, some components may have weak UV chromophores if UV detection is being used or be completely retained on the liquid chromatography column. (europeanpharmaceuticalreview.com)
  • Chromatographic separations were performed using a gradient program with a mixture of water (0.05% formic acid), as mobile phase A and acetonitrile (0.05% formic acid), as mobile phase B. Analytes quantification was performed by electro-spray ionisation-single quadrupole mass spectrometry using the selected ion recording (SIR) detection mode. (unito.it)
Tandem mass spectro1
  • A selective, sensitive, high pressure liquid chromatography-positive electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of aripiprazole in human K(2)EDTA plasma using zolpidem tartrate as an internal standard. (unboundmedicine.com)
Isolated by reversed phase1
Capillary1
  • It comprises three steps, i.e., release of glycans, derivatization with Fmoc, and capillary electrophoresis-electrospray ionization mass spectrometry (CE-ESI MS) analysis. (edu.au)
Analytical4
  • It is well known that most protein therapeutics such as monoclonal antibody pharmaceuticals and other biopharmaceuticals including cancer biomarkers are glycoproteins, and thus the development of high-throughput and sensitive analytical methods for glycans is essential in terms of their determination and quality control. (edu.au)
  • Liquid chromatography is an analytical technique utilised widely in the pharmaceutical industry. (europeanpharmaceuticalreview.com)
  • To guarantee that goods pass certain standards, researchers, manufacturers and developers use various technical equipment and analytical techniques , including liquid chromatography, during the development process. (europeanpharmaceuticalreview.com)
  • Liquid chromatography is an analytical technique that is used to separate a certain sample into its individual components. (europeanpharmaceuticalreview.com)
Analytes2
  • Separated analytes were determined in LC-MS single ion monitoring mode using an atmospheric pressure ionization-electrospray ionization (ESI) interface. (erowid.org)
  • Higher analytes concentrations in hydrolyzed samples showed the presence of conjugated compounds in urine. (erowid.org)
Selective1
  • We thus endeavor to understand how the pathogen adapts to the host microenvironment, what selective pressures are acting on the pathogen in each microenvironment, what bacterial factors are responsible for the host damage, and how the immune system is evaded. (cdc.gov)
Spectrometry3
Lipids3
  • It has been proposed that GDGT membrane lipids are an adaptation to the high temperatures present in the environments that are home to extremophile archaea Archaeal GDGTs were first detected in pelagic waters. (wikipedia.org)
  • Originally, it was believed that GDGT membrane lipids were an adaption to life at high temperatures and acidities. (wikipedia.org)
  • It has been proposed that the distinctive cyclohexyl group of crenarchaeol is an adaption to pelagic life, as it produces a "kink" in one of crenarchaeol's hydrocarbon chains that prevents the membrane lipids from packing tightly, as would be favorable under high temperatures but unfavorable under temperate ones. (wikipedia.org)
Reversed-phase1
  • Chromatography was performed on a C(18) reversed-phase column using a linear gradient of 10mM ammonium bicarbonate, pH 7.3 and acetonitrile as a mobile phase. (erowid.org)
Quantification1
  • Evaluation of a suspected hemoglobinopathy should include electrophoresis of a hemolysate to detect abnormal hemoglobins and quantification of HbA 2 and HbF with column chromatography. (medscape.com)
Method1
  • Here we have developed a high-throughput and highly sensitive method. (edu.au)
Journal2
  • Journal of Chromatography. (unboundmedicine.com)
  • AU - Lísa,Miroslav, AU - Holcapek,Michal, AU - Rezanka,Tomás, AU - Kabátová,Nadezda, Y1 - 2007/02/06/ PY - 2006/09/04/received PY - 2007/01/23/revised PY - 2007/01/25/accepted PY - 2007/2/20/pubmed PY - 2007/5/1/medline PY - 2007/2/20/entrez SP - 67 EP - 77 JF - Journal of chromatography. (unboundmedicine.com)
Affinity3
  • It is a high affinity scavenger for free hemoglobin (Hb) that is occasionally released from erythrocytes during hemolysis. (biomedcentral.com)
  • marmosets: rodents had higher levels with greater affinity than marmosets. (who.int)
  • a component with a higher affinity for the stationary phase will migrate down the column slower than a component which has more affinity for the mobile phase. (europeanpharmaceuticalreview.com)
Compounds2
  • Three sesquiterpene lactone compounds, 2 α ,5 α -dihydroxy-11 α H-eudesma-4(15)-en-12,8 β -olide ( 1 ), telekin ( 2 ), oxoeudesm-11(13)-eno-12,8 α -lactone ( 3 ) were obtained. (researchsquare.com)
  • All marker compounds showed higher fractions under dry conditions when formed during daytime and showed almost no RH effect when formed during night. (copernicus.org)
Rapid1
  • Surprisingly, the uptake of all three of the DNJ analogues was extremely rapid and was not dependent upon the length of the N-alk(en)yl moiety. (ox.ac.uk)
Column1
Retention2
  • TGs containing Delta5-UPIFAs have a higher retention in comparison with common TGs found in plant oils with single methylene interrupted Delta6(9)-FAs and also significantly changed relative abundances of fragment ions in APCI mass spectra. (unboundmedicine.com)
  • The present study further characterizes the properties of N-alk(en)ylated DNJs, and demonstrates that increasing the length of the side chain is a simple way of improving imino sugar retention and therefore inhibitory efficacy for CGT in cultured cells. (ox.ac.uk)
Biomarkers1
Inhibitors1
  • Accordingly, the present invention provides novel JAK inhibitors which are both potent JAK inhibitors, and in particular TYK2/JAK1 inhibitors, but also display high clearance in human hepatocytes, thus affording significantly improved systemic clearance, and thereby reducing the risk of adverse side effects upon topical administration while retaining efficacy in the skin. (justia.com)
Concentrations2
  • Most GDGTs degrade at between 240 and 300 °C and so are not found in rocks that have undergone heating to temperatures higher than 300 °C. GDGTs undergo degradation when exposed to oxygen but the relative concentrations of sediment GDGTs tends to remain the same even during degradation, meaning that degradation does not interfere with proxies like TEX86 that are based on the ratios of different GDGTs. (wikipedia.org)
  • Thus, MEHP and perhaps 2-EHA and other di-carboxylic acid concentrations in the body were higher in mice or rats than marmosets when the same dose of DEHP was administered (Ito et al. (who.int)
Triacylglycerols2
  • Spectral identification of the triacylglycerols was based on the diacylglycerol fragments and on the protonated molecular ion [M+H]**+, except trisaturates which gave no [M+H]**+. Triacylglycerols were identified and quantitated in normal, high stearic acid and high lauric acid canola varieties. (usda.gov)
  • The oils of the modified canola varieties, compared to typical canola oil, contained increased content of triacylglycerols known to be more oxidatively stable like stearoyloleoyllinoleoyl, distearoyllinoleoyl, stearoyldioleoyl and distearoyloleoyl glycerols in high stearic acid canola oil and dilauroyllinoleoyl, dilauroyloleoyl and lauroyldioleoyl glycerols in high lauric acid canola oil. (usda.gov)
System1
  • The success of the P. pastoris expression system is partly attributed to its ability to grow to high cell densities and as such increasing its volumetric productivity. (biomedcentral.com)
Development1
  • This study aimed to explore the metabolic effects of two carbohydrate sources with different degrees of risk in obesity development when associated with a high fat intake. (mdpi.com)
Mode1
  • e multiple reaction monitoring mode was chosen with an electrospray ionization source operating in the positive ionization mode for data acquisition. (sagepub.com)
Prevalence2
  • Population prevalence was higher in females (1.4%-2.2%) than males. (cdc.gov)
  • In this cross-sectional study in Tehran, Islamic Republic of Iran, a country with a high prevalence of vitamin D deficiency, we determined the prevalence of vitamin D deficiency among 90 type 2 DM patients and 90 healthy subjects. (who.int)
Analysis1
  • Modern Food Microbiology , Seventh Edition builds on the trusted and established sections on food preservation by modified atmosphere, high pressure and pulsed electric feiled processing, food-borne pathogens, food regulations, fresh-cut produce, new food products, and risk assessment and analysis. (dattanibookagency.com)
Type3
  • RÉSUMÉ Une relation inverse a été démontrée entre le déficit en vitamine D et le diabète de type 2. (who.int)
  • Dans le cadre de la présente étude transversale menée à Téhéran (République islamique d'Iran), dans un pays où la prévalence du déficit en vitamine D est élevée, nous avons mesuré cette prévalence chez 90 patients souffrant d'un diabète de type 2 et 90 sujets en bonne santé. (who.int)
  • La prévalence du déficit en vitamine D chez les hommes atteints d'un diabète de type 2 était de 42,7 %, contre 22,2 % chez les hommes en bonne santé. (who.int)
Generally1
  • Environmental concerns and the quest for sustainability create a pressure to generally minimize the use of chemicals and to replace those that are environmentally questionable with alternatives that are low-toxic and based on renewable resources. (lu.se)
Positive1
  • The Quantitative Measurement of Peptidoglycan Components Obtained from Acidic Hydrolysis in Gram-Positive and Gram-Negative Bacteria via Hydrophilic Interaction Liquid Chromatography Coupled with Mass Spectrometr. (tftak.eu)
Quality2
  • In the pharmaceutical industry, all manufactured products need to be of the highest quality to ensure the least risk to patients. (europeanpharmaceuticalreview.com)
  • For therapeutic applications, immunoglobulins must be of very high product quality. (biomedcentral.com)
Patients1
  • 50 nmol/L) et de l'insuffisance en vitamine D (50-75 nmol/L) était respectivement de 59,0 % et de 27,0 % chez les patients diabétiques, contre 47,0 % et 24,0 % chez les sujets en bonne santé. (who.int)
Similar2
  • A high-risk (HR) high-fat diet containing 20% fructose was compared to a control lower-risk (LR) high-fat diet where a similar amount of carbohydrate was provided as a mix of digestible and resistant starch from high amylose maize. (mdpi.com)
  • A similar effect was observed in male rats (flow 150 at CDC Public Health Library & Information Center on July 11, 2012 molecule with a vapor pressure of 0.09 torr at 25 C, a log ml/min) with uptake efficiencies of 57, 49, 37, and 36% being observed. (cdc.gov)
Blood1
  • It is also used for conditions of the heart and blood vessels including poor circulation, excessive blood clotting, high cholesterol , and preventing heart disease . (medicinenet.com)
Expression1
  • The high expression of this pro-inflammatory protein is probably related to the inflammatory response and to the altered neutrophil responses to functional stimuli that characterize SAPHO syndrome suggesting a possible application as a salivary biomarker. (unicatt.it)
Study1
  • We have synthesized a series of DNJ analogues to study the contribution of N-alk(en)yl side chains (C4, C9 or C18) to the behaviour of these analogues in cultured HL60 cells. (ox.ac.uk)