• This study examined nine detergents upon nAChR solubilization and purification, to assess receptor lipid composition using GC (Gas Chromatography)-FID (Flame Ionization) and/or GC-MSD (Mass Selective Detectors), stability and aggregation state using A-SEC (Analytical Size-Exclusion Chromatography) and EM (Electron Microscopy), and planar lipid bilayers to measure ion channel function. (upr.edu)
  • The Acid-sensing Ion Channel (ASIC) detects changes in pH levels in the organism and transmits the pain signal to the brain. (scienmag.com)
  • Purpose: The purpose of the current study is to assess changes in the expression of Acid-Sensing Ion Channel (ASIC)1a and ASIC2 in retinal ganglion cells (RGCs) after retinal ischemia and reperfusion (I/R) injury and to test if inhibition of ASIC1a provides RGC neuroprotection. (unthsc.edu)
  • Current flow through the channel formed in planar phospholipid bilayer membranes by the PA 65 fragment of anthrax toxin is blocked, in a voltage-dependent manner, by tetraalkylammonium ions (at micromolar concentrations), which bind to a blocking site within the channel lumen. (elsevier.com)
  • Blaustein, RO & Finkelstein, A 1990, ' Diffusion limitation in the block by symmetric tetraalkylammonium ions of anthrax toxin channels in planar phospholipid bilayer membranes ', Journal of General Physiology , vol. 96, no. 5, pp. 943-957. (elsevier.com)
  • We have presented evidence that diffusion plays a significant role in the kinetics of blocking by tetrabutylammonium ion (Bu 4 N + ) from the cis (toxin-containing) side of the membrane (Blaustein, R. O., E. J. A. Lea, and A. Finkelstein. (elsevier.com)
  • Anapoe-C12E9 (Polyoxyethylene-(9)-dodecyl ether) and BigCHAP (N,N′-bis-(3-d- Gluconamidopropyl) cholamide) retained residual amounts of native lipid, maintaining moderate stability and ion channel function when compared to lipid-analog detergents. (upr.edu)
  • The research team identified through animal experiments that there is a different cell membrane merging mechanism between subunits of the Acid-sensing Ion Channel. (scienmag.com)
  • The outcome of this study is meaningful since it identified a new cell membrane merging mechanism of the Acid-sensing Ion Channel, and furthermore is significant that it proposed a research direction for a new understanding of the activity control mechanism of various ion channels among subunits including ASIC2, ASIC2b and many other subunits. (scienmag.com)
  • Professor Byung-Chang Suh from DGIST's Department of Brain and Cognitive Sciences said, 'Understanding of the cell membrane merging and activity control mechanism of the Acid-sensing Ion Channel plays an important role in identifying the pain signal transmission system. (scienmag.com)
  • Professor Byung-Chang Suh's research team from the Department of Brain and Cognitive Sciences succeeded in identifying a new operational mechanism principle of the 'Acid-sensing Ion Channel,' which recognizes internal pain in an organism. (scienmag.com)
  • The findings are expected to have a significant impact on further studies focusing on the development of therapeutic agents that control pain by providing a more precise understanding of the operational mechanism of the 'Acid-sensing Ion Channel' that plays a pivotal role in transmitting pain signals. (scienmag.com)
  • That our study investigated a new control mechanism of the Acid-sensing Ion Channel has important implications. (scienmag.com)
  • Acid-Sensing Ion Channel 2a (ASIC2a) Promotes Surface Trafficking of ASIC2b via Heteromeric Assembly', Scientific Reports 2016. (scienmag.com)
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