The hypothesis of the present study is that apoptosis through an intrinsic mitochondrial pathway may mediate high fat diet (HFD)-induced changes in the metabolism of Pelteobagrus fulvidraco. (figshare.com)
To this end, we cloned the full-length cDNA sequences of Cycs, Apaf1, Casp9, Casp3a, and Casp3b involved in the mitochondria apoptotic pathway, and explored their mRNA tissue expressions and transcriptional responses to HFD. (figshare.com)
The 'intrinsic' pathway is activated mainly by non-receptor stimuli, such as DNA damage, ER stress, metabolic stress, UV radiation or growth-factor deprivation. (kegg.jp)
The central event in the 'intrinsic' pathway is the mitochondrial outer membrane permeabilization (MOMP), which leads to the release of cytochrome c. (kegg.jp)
Punicic acid, an important fatty acid in pomegranate seeds, was further found to induce intrinsic apoptosis via a caspase-dependent pathway. (nih.gov)
Here we demonstrate that, during brain maturation, the potential of the intrinsic apoptotic pathway is progressively reduced and that such repression is associated with downregulation of apoptotic protease-activating factor-1 (Apaf-1) and caspase-3 gene expression. (jneurosci.org)
These results suggest that differential expression of Apaf-1 and caspase-3 genes may underlie regulation of apoptotic susceptibility during brain development, as well as after acute injury to mature brain, through the intrinsic pathway of caspase activation. (jneurosci.org)
Eldadah and Faden, 2000 ), we hypothesized that suppression of apoptotic capability during maturation of mammalian brain results from repression of genes involved in the caspase-3 activation pathway, and that injury-induced neuronal apoptosis in the mature brain results from reactivation of these genes. (jneurosci.org)
In the present study, we evaluated the role of the intrinsic pathway in neuronal apoptosis at different stages of rat brain development and during maturation of primary cortical neurons in vivo as well as in response to brain injury. (jneurosci.org)
Eiger is produced in apoptotic cells and, through activation of the c-Jun N-terminal kinase (JNK) pathway, is able to propagate the initial apoptotic stimulus. (elifesciences.org)
Caspase-8/Bid and mitochondrial signaling may play a major role in TiO2 nanoparticle -induced apoptosis involving the intrinsic mitochondrial pathway. (cdc.gov)
Furthermore, C3G regulated the intrinsic apoptotic pathway-associated proteins, such as proteins belonging to the Bcl-2 family, cytochrome c and caspase-3. (spandidos-publications.com)
The intrinsic pathway is engaged by a variety of intracellular signals and leads to the activation of Caspase-9. (invivogen.com)
They are further implicated to play a major role during the intrinsic apoptotic pathway. (db-engine.de)
It was hypothesised that manipulation of this clearance pathway, which is normally employed to prevent autoimmunity, may alleviate immuno-suppression of apoptotic tumour cells and promote the expansion of a tumour antigen-specific T cell response. (soton.ac.uk)
Loading BMDCs with apoptotic cells via a PS-independent pathway or through Fc? (soton.ac.uk)
BCL2 proteins are described as 'crucial regulators of apoptosis' (PMID:15868100)and the intrinsic apoptosis pathway is initiated by the release of mitochondrial cytochrome c into the cytoplasm, which results in the activation of caspases (15102863) - help in generating a defintion for intrinsic apoptosis? (geneontology.org)
The intrinsic apoptotic pathway is characterized by permeabilisation of the mitochondria and release of cytochrome c into the cytoplasm. (geneontology.org)
The intrinsic apoptotic pathway has been chosen for a Reference Genome project as the curation work will complement and inform a planned apoptosis content meeting scheduled for June the 1st, assisted by domain experts from the Apo-Sys Consortium. (geneontology.org)
In addition, as the intrinsic apoptotic pathway seems to have evolved at the same time as multicellular organisms, whereas the extrinsic pathway is a more recent evolutionary development in veterbrates, it is felt the intrinsic mechanism was more suited to a multi-organism curation project. (geneontology.org)
[ 2 ] Other ALPS-associated genetic defects in the apoptotic pathway have since been identified. (medscape.com)
An NRAS mutation has been identified that represents the first identified case of ALPS resulting from a defect in the intrinsic, or mitochondrial, apoptotic pathway. (medscape.com)
Anti-apoptotic6
It is the balance between the pro-apoptotic and anti-apoptotic signals that eventually determines whether cells will undergo apoptosis, survive or proliferate. (kegg.jp)
TNF family of ligands activates anti-apoptotic or cell-survival signals as well as apoptotic signals. (kegg.jp)
While it is well understood that doxorubicin activates p53 and induces cell-cycle arrest, we observed that cell-cycle arrest disabled inactivation of anti-apoptotic Bcl-2-family members by vincristine. (uni-muenchen.de)
p53 can act pro- and anti-apoptotic, depending on the individual patient tumor cell and on drug and signal interactions. (uni-muenchen.de)
SRSF1 is upregulated in a variety of cancers, promoting and inhibiting pro-proliferative versus anti-apoptotic mRNA splice variants, respectively. (forschung-sachsen-anhalt.de)
PMID:14996496, table 1 provides links to papers that describe subcellular location of many pro- and anti-apoptotic proteins. (geneontology.org)
Pathways5
In particular, we will discuss the modifications in intrinsic and extrinsic apoptotic pathways caused by proteins encoded by HPV early genes. (mdpi.com)
Chang CY, Shen CY, Kang CK, Sher YP, Sheu WH, Chang CC, Lee TH (2014) Tau protects HK-2 cells from oxidized LDL-induced cytotoxicity via the ROS-mediated mitochondrial and p53-related apoptotic pathways. (springer.com)
Furthermore, immunoblotting studies revealed the activation of intrinsic and maybe extrinsic apoptosis pathways by OEO/thymol. (irost.org)
Two primary apoptosis pathways exist, the intrinsic and extrinsic pathways. (invivogen.com)
The caspases can be activated through either the intrinsic (mitochondrial mediated) or extrinsic (death receptor mediated) apoptotic pathways. (geneontology.org)
Extrinsic apoptosis2
We investigated the importance of p53 in the intrinsic and extrinsic apoptosis signaling, via a variety of molecular biological approaches (Wachter et al. (uni-muenchen.de)
Pannexin-1 (Panx1) channels mediate the efflux of ATP and AMP from cancer cells in response to induction of extrinsic apoptosis by death receptors or intrinsic apoptosis by chemotherapeutic agents. (aspetjournals.org)
Low ATP concentrations in hypoxic tumour cells disable the apoptotic cascade and induce cell death by necrosis. (bmj.com)
Failure of the immune system to differentiate between apoptotic tumour cells and cells rendered apoptotic as part of homeostasis prevents a successful response being mounted against tumours. (soton.ac.uk)
MRNA2
Total RNA was isolated on P16, and the relative abundance of mRNA of the crystallin structural genes, redox components, and apoptotic cascade were ascertained with real-time PCR with reference to the internal control β-actin . (molvis.org)
C-PC treatment possibly prevented cataractogenesis triggered by sodium selenite, by regulating the lens crystallin, redox genes, and apoptotic cascade mRNA expression and thus maintains lens transparency. (molvis.org)
Mitochondria1
Further studies demonstrated that TiO2 nanoparticle s induced significant changes in mitochondrial membrane permeability, suggesting the involvement of mitochondria in the apoptotic process. (cdc.gov)
Proteins3
Taken at face value, senile cataract is intimately associated with the increased risk of oxidative stress, which levies irrevocable changes in the lenticular proteins that impel the lens epithelial cells to the apoptotic phase. (molvis.org)
2013). Apoptosis is induced intrinsically by the release of pro-apoptotic proteins from the inter-membrane space of the mitochondrion (eg. (uni-muenchen.de)
Here, two recombinant dominant-negative MFG-E8 proteins were generated: one which is shown to inhibit PS-dependent apoptotic cell uptake by macrophages by over 40% (DN-MFG-E8), and a second which re-directed apoptotic cells through Fc? (soton.ac.uk)
Mechanisms7
To avoid elimination by this mechanism, human papillomaviruses (HPV) have developed several mechanisms that enable the cells they infect to elude both extrinsic and intrinsic apoptosis. (mdpi.com)
Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
In conclusion, punicic acid, the main constituent of pomegranate seed (70-80%), exhibited potent growth inhibitory activities in androgen-dependent LNCaP cells, which appear to be mediated by both antiandrogenic and pro-apoptotic mechanisms. (nih.gov)
However, the molecular mechanisms underlying such age-dependent differences in apoptotic response to neuronal injury have not been identified. (jneurosci.org)
This review discusses the recent findings regarding sarcopenia, the intrinsic, and extrinsic mechanisms involved in the onset and progression of this disease and the treatment approaches that have been developed based on testosterone deficiency and their implications. (frontiersin.org)
ALPS, as this disorder was subsequently named, has been shown to represent a failure of apoptotic mechanisms. (medscape.com)
Environmental factors that can detect specific IgE antibodies against most low-molecular- affect the initiation of occupational asthma include the intrinsic weight agents has resulted in a search for alternative or characteristics of causative agents as well as the influence of the complementary physiopathologic mechanisms leading to airway level and route of exposure at the workplace. (cdc.gov)
Caspases2
Kadeyala PK, Sannadi S, Gottipolu RR (2013) Alterations in apoptotic caspases and antioxidant enzymes in arsenic exposed rat brain regions: reversal effect of essential metals and a chelating agent. (springer.com)
Apoptotic signaling is initiated either through extrinsic or intrinsic stimulation, resulting in the activation of caspases [ 12 ]. (biomedcentral.com)
Maturation1
receptors (Fc?Rs) reduced their ability to induce CD8+ T cell proliferation in vivo, suggesting the blockade of a mechanism which is intrinsic for DC maturation or migration. (soton.ac.uk)
Caspase1
Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. (irost.org)
Tumor2
Tumor-intrinsic sensitivity to the pro-apoptotic effects of IFN-γ is a major determinant of CD4+ CAR T-cell antitumor activity. (pasteur.fr)
We propose that the sensitivity of tumor cells to the pro-apoptotic activity of IFN-γ is a major determinant of CD4+ CAR T-cell efficacy and may be considered to guide the use of CD4+ T cells during immunotherapy. (pasteur.fr)
Cells22
However, the ability of HPV-infected cells to resist apoptotic signals renders such therapies ineffective. (mdpi.com)
These findings suggest that inter- the apoptotic destruction of lymphocytes and dendritic ventions aimed at reducing the extent of immune cell apop- tosis could improve outcomes for a variety of severe cells could have a particularly adverse effect on disease human infections, including those caused by emerging outcome. (cdc.gov)
How do C-type lectins tailor adaptive immunity following phagocytosis of apoptotic cells? (nature.com)
In this study, we investigated the growth inhibitory, antiandrogenic, and pro-apoptotic effects of 13 pure compounds found in the pomegranate in androgen-dependent LNCaP human prostate cancer cells. (nih.gov)
Apoptotic cells can produce signals to instruct cells in their local environment, including ones that stimulate engulfment and proliferation. (elifesciences.org)
We identified a novel mode of communication by which apoptotic cells induce additional apoptosis in the same tissue. (elifesciences.org)
We also show that during coordinated cell death of hair follicle cells in mice, TNF-α is expressed in apoptotic cells and is required for normal cell death. (elifesciences.org)
Although this process can occur in individual cells, apoptotic signals may also dictate the 'communal death' of many cells simultaneously. (elifesciences.org)
Apoptosis has been thought to resemble a form of silent suicide by cells, but more recent work suggests that apoptotic cells can also transmit signals. (elifesciences.org)
To explore whether apoptotic cells could communicate with other cells, Pérez-Garijo et al. (elifesciences.org)
found that TNF is produced by apoptotic cells in hair follicles, and that blocking TNF inhibits the death of other cells in the same cohort: this suggests that a common mechanism could regulate the communal death of cells in flies and mammals. (elifesciences.org)
Apoptosis is a distinct form of programmed cell death in which cells activate an intrinsic suicide program to self-destruct. (elifesciences.org)
Only more recently has it become clear that apoptotic cells are the source of signals that can have profound effects on their neighbors. (elifesciences.org)
In conclusion, evidence indicated that TiO2 nanoparticle s exhibit higher cytotoxicity and apoptotic induction compared to fine particles in JB6 cells. (cdc.gov)
The severity of hypoxia determines whether cells become apoptotic or adapt to hypoxia and survive. (bmj.com)
3- 5 Therefore, during severe hypoxia or anoxia, the cell initiates a cascade of events that leads to apoptotic cell death, thereby preventing the accumulation of cells with hypoxia induced mutations. (bmj.com)
Thus, Notch signalscontrol how cells respond to intrinsic or extrinsic developmental cues that arenecessary to unfold specific developmental programs. (embl-heidelberg.de)
Robust extracellular ATP/AMP accumulation was observed in the FADD-deficient cells during necroptosis, but not during apoptotic activation of Panx1 channels. (aspetjournals.org)
Accumulation of extracellular ATP/AMP was similarly absent in RIP1-deficient Jurkat cells during apoptotic responses to chemotherapeutic agents. (aspetjournals.org)
Apoptotic cells are cleared rapidly by professional phagocytes thus preventing the release of potentially inflammatory or immunogenic material into the surrounding environment. (soton.ac.uk)
It facilitates the uptake of apoptotic cells by acting as a bridging molecule between integrins on the phagocyte and phosphatidylserine (PS) on the apoptotic cell surface. (soton.ac.uk)
Apoptotic cells treated with DN-MFG-E8-Fc protein resulted in upregulation of costimulatory molecules, CD86 and CD70, when BMDCs were deficient for the inhibitory Fc?R, Fc?RIIb. (soton.ac.uk)
Genes1
Real-time PCR analysis showed the crystallin genes ( αA- , βB1- , γD- ) and redox cycle components ( Cat , SOD-1 , Gpx ) were downregulated, the apoptotic components were upregulated, and antiapoptotic Bcl-2 was downregulated in Group II. (molvis.org)
Proliferation1
Notch activity affects theimplementation of differentiation, proliferation, and apoptotic programs,providing a general developmental tool to influence organ formation andmorphogenesis. (embl-heidelberg.de)
In addition to its importance as a biological phenomenon, defective apoptotic processes have been implicated in an extensive variety of diseases. (geneontology.org)
Cell4
In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. (irost.org)
Yet, NOX participates in cellular signaling in a cell-intrinsic and -extrinsic manner, e.g., via the release of ROS into the extracellular space. (frontiersin.org)
As described in the literature, wildtype p53 leaded to an increase in intrinsic cell death, whereas different p53 mutations reduced apoptosis. (uni-muenchen.de)
Apoptotic activation triggered equivalent proteolytic gating of Panx1 channels in all three Jurkat cell lines. (aspetjournals.org)
Cancers1
Work by our group revealed that many KRAS mutant lung cancers exhibit decreased oncogenic dependency and a dampened apoptotic response that contributes to intrinsic resistance to KRAS targeted therapy. (massgeneral.org)
Occur1
Indeed, most of the intrinsic as well as extrinsic (systemic) muscle changes that occur with age are believed to be involved in the development of sarcopenia ( 5 , 6 ). (frontiersin.org)
Shown1
In summary we have shown the heterogeneity of p53 in extrinsic and intrinsic apoptosis signaling. (uni-muenchen.de)
Rate1
The apoptotic rate of mouse hippocampus was determined by TUNEL staining. (springer.com)
Expression1
A similar decline in apoptotic susceptibility associated with downregulation of Apaf-1 expression as a function of developmental age was also found in cultured primary rat cortical neurons. (jneurosci.org)