Intracellular pathogen brucella. Medical search. Frequent questions

Brucellosis is a worldwide zoonosis caused by gram-negative, intracellular Brucella coccobacilli. (cdc.gov)
Brucellosis is a common zoonotic disease caused by intracellular pathogens of the genus Brucella . (frontiersin.org)
Bacteria of the genus Brucella are the causative agents of brucellosis, a worldwide zoonosis that affects a broad range of mammals, including livestock and humans. (mgc.ac.cn)
Interest in brucellosis has been increasing because of the growing phenomena of international tourism and migration, in addition to the potential use of Brucella as a biological weapon. (medscape.com)
Brucella abortus is a Gram-negative, [alpha]-proteobacterium that causes bovine brucellosis, a prevalent zoonotic disease in many areas of the world. (ecu.edu)
Background: Brucella is a facultative intracellular pathogen responsible for zoonotic disease brucellosis. (ntu.ac.uk)
These are novel findings that offer insight into understanding the interplay between Brucella and host target cells, which may aid in future identification of a new target for diagnosis and/or vaccine development and prevention of brucellosis. (ntu.ac.uk)
Brucella abortus is a Gram negative intracellular pathogen that causes the zoonotic disease brucellosis. (ecu.edu)
Brucella melitensis and Brucella ovis are gram-negative pathogens of sheep that cause severe economic losses and, although B. ovis is non-zoonotic, B. melitensis is the main cause of human brucellosis. (biomedcentral.com)
Brucellosis is a worldwide extended disease caused by gram-negative bacteria of the genus Brucella that has a severe impact on animal and human health [ 1 ]. (biomedcentral.com)
Here, at the National Animal Disease Center, we study infections of livestock with species like brucella abortus, which causes brucellosis in cattle and bison, and E.Coli O157:H7 as shed from the intestinal tracks of cattle. (jove.com)
Brucella abortus (B. abortus) is a facultative intracellular pathogen that can survive inside macrophages and trophoblast giant cells, and the causative agent of brucellosis. (elsevierpure.com)
Brucellosis is caused by facultative intracellular pathogens of the genus Brucella that have domestic animals, mainly goats, sheep and cows, as natural reservoirs. (bridgingproject.eu)
Here, we show that Brucella , the causative agent of brucellosis, the most prevalent bacterial zoonosis globally, subverts this immune defense pathway by activating regulated IRE1α-dependent decay (RIDD) of Bloc1s1 mRNA encoding BLOS1, a protein that promotes endosome-lysosome fusion. (nsf.gov)
Brucellosis is a widespread zoonosis of great economic importance caused by facultative intracellular Gram-negative bacteria belonging to the genus Brucella . (biomedcentral.com)
Brucellosis is a widespread and economically important infectious disease of humans and animals caused by members of the genus Brucella . (biomedcentral.com)
When humans come in contact with an infected animal or animal products that are contaminated with Brucella, brucellosis may result. (loinc.org)
The current focus of brucellosis control is on surveillance, with rapid elimination of new Brucella infections. (veteriankey.com)

Brucella infects macrophages and evades clearance mechanisms, thus resulting in chronic parasitism. (frontiersin.org)
RNA sequencing was performed in primary human macrophages (Mφ) and polymorphonuclear neutrophils (PMNs) infected with a clinical strain of Brucella spp. (frontiersin.org)
Transformation of znuA mutant with a shuttle vector pBBR1MCS-4 containing znuA gene restored the growth in zinc chelated medium and intracellular replication in HeLa cells and macrophages to a level comparable to that of wild-type strain. (go.jp)
Once within the bloodstream, the organisms quickly become intracellular pathogens contained within circulating polymorphonuclear cells (PMNs) and macrophages, making use of numerous mechanisms to avoid or suppress bactericidal responses. (medscape.com)
Research carried out with the participation of the University of Navarra has shown how a determinate molecule helps an important pathogen, Brucella abortus, escape destruction within the cells charged with eliminating infectious agents (macrophages). (sciencedaily.com)
Brucella penetrates the macrophages within membranous vesicles that are not fused with lysosomes (structures containing cellular products necessary to destroy bacteria) as occurs in other micro-organisms. (sciencedaily.com)
In contrast to wild type organisms, Brucella T4SS mutants were completely defective in their ability to growth inside of macrophages. (kirbylab.org)
The IRE1α-XBP1 signaling axis supports this glycolytic switch in macrophages when activated by lipopolysaccharide (LPS) stimulation or infection with the intracellular bacterial pathogen Brucella abortus. (biomed.news)
When confronted with bacterial pathogens, macrophages, frontline defenders in the immune system, switch to a glycolysis-driven metabolism to carry out their antibacterial functions. (biomed.news)
The dead host cell then tricks other, still-living macrophages into becoming the new hosts for the pathogens. (gutnews.com)
Macrophages only live about 30 days, however, so intracellular bacteria like Salmonella must periodically find new host cells. (gutnews.com)

Targeting intracellular pathogenic bacteria with unnatural proline-rich peptides: coupling antibacterial activity with macrophage penetration. (harvard.edu)
Brucella is a model of an intracellular parasite, a category that includes other important bacteria, such as those of tuberculosis or legionelosis. (sciencedaily.com)
One of my favorite figures are images of tdTomato-labeled Brucella detected by confocal microscopy replicating in the middle of a liver granulomas visualized by counterstaining with H&E. In contrast to wild type organisms, virB4 mutant bacteria are found as single cells in sinusoids presumably within Kuffpfer cells. (kirbylab.org)
Red objects are individual Brucella neotomae bacteria. (kirbylab.org)
We found that there was a higher degree of bacterial colonization in the placenta than in other organs, that there were many bacteria in trophoblast giant (TG) cells in the placenta and that an intracellular replication-defective mutant did not induce abortion. (biomedcentral.com)
A commercial biotyping system (Taxa Profile™, Merlin Diagnostika) testing the metabolization of various substrates by bacteria was used to determine if a set of phenotypic features will allow the identification of members of the genus Brucella and their differentiation into species and biovars. (biomedcentral.com)
Based on specific and stable reactions a 96-well " Brucella identification and typing" plate (Micronaut™) was designed and re-tested in 113 Brucella isolates and a couple of closely related bacteria. (biomedcentral.com)
Salmonella is just one type of intracellular bacteria that can cause disease in people. (gutnews.com)
In the coming decades, antibiotic resistance is expected to increase significantly, meaning that the antibiotics available may no longer be effective against intracellular bacteria. (gutnews.com)
Chloramphenicol is a broad-spectrum antibiotic, inhibiting gram-positive and gram-negative organisms, aerobic and anaerobic bacteria, and many intracellular organisms. (veteriankey.com)
Most Gram-negative bacteria are considered to be harmful to their hosts, many of them (such as Brucella melitensis , Salmonella enterica and Escherichia coli ) are pathogenic to both animals and humans. (biomedcentral.com)
Non-point source pollution including excess nutrients, organic particles, fecal coliform bacteria, and additional pathogens is considered high risk at many animal operations, especially equine facilities. (umass.edu)

It is caused by various species of the bacterial genus Brucella , which mainly infect domestic animals, especially goats, sheep, and cows, and use them as natural reservoirs. (frontiersin.org)
A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. (harvard.edu)
The genus Brucella consists of six classic species, designated on the basis of host preference, antigenic and biochemical characteristics as Brucella melitensis (goats and sheep), Brucella abortus (cattle), Brucella suis (pigs), Brucella canis (dogs), Brucella ovis (sheep) and Brucella neotomae (wood rats). (mgc.ac.cn)
The comprehensive testing of metabolic activity allows cluster analysis within the genus Brucella . (biomedcentral.com)
The aim of our study was to develop a miniaturised semi-automated system for the reliable identification of members of the genus Brucella and the differentiation of its species based on comprehensive metabolic activity testing. (biomedcentral.com)
Brucella abortus is a species of the genus Brucella. (loinc.org)

Facultative intracellular pathogens are able to live and reproduce either inside or outside of host cells. (msdmanuals.com)
are facultative intracellular pathogens that have the ability to survive and multiply in professional and nonprofessional phagocytes, and cause abortion in domestic animals and undulant fever in humans. (go.jp)
are small, gram-negative, facultative intracellular pathogens that cause abortion, and are retained in the placenta and causing infertility in numerous domestic and wild mammals. (biomedcentral.com)

Brucella abortus depends on pyruvate phosphate dikinase and malic enzyme but not on Fbp and GlpX fructose-1,6-bisphosphatases for full virulence in laboratory models. (harvard.edu)
Its virulence depends on survival and replication properites in different cell types in which brucella controls the maturation of its vacuole to avoid innate immune responses and to reach its replicative niche associated with the endoplasmic reticulum. (mgc.ac.cn)
2008. Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes. (mgc.ac.cn)
Written and edited by experts in the field, this collection from Cold Spring Harbor Perspectives in Medicine provides a comprehensive review of the biology of these pathogens, their virulence mechanisms, and the host's response to infection. (cshlpress.com)
The researchers demonstrated that Brucella uses the same mechanism as Salmonella to evade the host immune system, suggesting that this virulence strategy may be shared by other bacterial pathogens that can live within host cells. (gutnews.com)

Brucella is a Gram-negative bacterium which is pathogenic of human and animals [ 11 ]. (hindawi.com)
2005. Whole-genome analyses of speciation events in pathogenic Brucellae . (mgc.ac.cn)
2011. Interactions of the human pathogenic Brucella species with their hosts. (mgc.ac.cn)
The main pathogenic species of Brucella are Brucella melitensis, Brucella suis and Brucella abortus. (loinc.org)

B Lymphocytes provide an infection niche for intracellular bacterium Brucella abortus. (harvard.edu)
Brucella melitensis is a gram-negative coccobacillus bacterium from the Brucellaceae family. (loinc.org)
The facultative, intracellular bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. (biomedcentral.com)

Brucella organisms, which are small aerobic intracellular coccobacilli, localize in the reproductive organs of host animals, causing abortions and sterility. (medscape.com)
Fascinatingly co-infection with Legionella pneumophila , another T4SS-dependent pathogen, was able to rescue intracellular growth of the T4SS-mutant Brucella and also stimulated growth of wild type Brucella organisms! (kirbylab.org)

When a neutrophil arrives to clean up the dead macrophage with the Salmonella inside, it engulfs both the dead macrophage and the pathogen in a process called efferocytosis . (gutnews.com)

Brucellae are aerobic gram-negative coccobacilli that possess a unique ability to invade both phagocytic and nonphagocytic cells and to survive in the intracellular environment by finding ways to avoid the immune system. (medscape.com)
Brucella are gram-negative coccobacilli that commonly infect cows (Brucella abortus), pigs (Brucella suis), goats (Brucella melitensis), and dogs (Brucella canis), along with wild animals such as deer, elk, and moose. (loinc.org)
B. suis are gram-negative, facultative intracellular coccobacilli and can specifically produce in phagocytic cells. (loinc.org)
Its cells are nonmotile coccobacilli and are animal parasites and pathogens. (loinc.org)

The precise epidemiology, pathogenesis, and zoonotic potential of Brucella in amphibians remains largely unknown. (cdc.gov)

2002. The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. (mgc.ac.cn)
2005. Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis . (mgc.ac.cn)
Brucella suis infections in pigs causes chronic inflammatory lesions in the reproductive organs or orchitis and may affect joints and/or other organs. (loinc.org)
Brucella suis are differentiated into five strains. (loinc.org)

Species and biovar classification of brucellae is historically based on natural host preference and phenotypic traits, i.e. (biomedcentral.com)

Both Brucella isolates grew on MacConkey, Thayer-Martin, and blood agar at both 37°C and ambient conditions. (cdc.gov)
Due to their typical metabolic profiles a differentiation of Brucella isolates to the species level could be achieved. (biomedcentral.com)
Clinical isolates of the gastric pathogen Helicobacter pylori display a high level of genetic macro- and microheterogeneity, featuring a panmictic, rather than clonal structure. (biomedcentral.com)

Brucella abortus is a facultative intracellular gram-negative bacterial pathogen that causes abortion in pregnant cattle and undulant fever in humans. (ac.ir)
The uptake of abortion-inducing pathogens by trophoblast giant (TG) cells is a key event in infectious abortion. (biomedcentral.com)
The infectious abortion model using pregnant mice is a powerful tool for investigating the mechanisms of pathogen infection. (biomedcentral.com)

He then validated a type IV secretion system-dependent Brucella model using the wood rat pathogen, Brucella neotomae . (kirbylab.org)

This discovery not only means new useful ideas for other researchers, but also the enhanced knowledge of a very important pathogen. (sciencedaily.com)
Helicobacter pylori ( H. pylori) is an important pathogen of paediatric gastroenterology [1, 2]. (webmedcentral.com)

Obligate intracellular pathogens are able to grow, reproduce, and cause disease only within the cells of the host. (msdmanuals.com)
Obligate intracellular pathogens (e.g. (amboss.com)

Historically, our research has focused on important tick-transmitted pathogens in the genera Anaplasma, Babesia, Borrelia, Ehrlichia and Rickettsia. (ncsu.edu)

Two of these ( B. ceti , B. pinnipedialis ) are also considered atypical Brucella species similar to B. microti and B. inopinata ( 1 ). (cdc.gov)
Brucella ceti (cetaceans) and Brucella pinnipedialis (seals). (msdvetmanual.com)
Brucella ceti has also been documented in a traumatic nonhealing wound in a sea otter. (msdvetmanual.com)

Aerobic facultative intracellular pathogen. (mgc.ac.cn)

Some other pathogens that live inside cells include Brucella abortus , Listeria monocytogenes , Chlamydia trachomatis , Coxiella burnetii and Mycobacterium tuberculosis . (gutnews.com)

Using Nod-deficient mice, several in vivo studies have demonstrated a role for Nod1 and Nod2 in host defense against pathogens such as Helicobacter pylori, Listeria monocytogenes, Staphylococcus aureus, and Legionella pneumophila [ 7 - 10 ]. (hindawi.com)

A Genome Wide Screen Defines Macrophage Genes Modulating Intracellular Control of Mycobacterium abscessus Following Antibiotic Treatment. (asm.org)

With infection by wild type B. abortus, a large amount of RANTES production was observed in pregnant IFN-γ knockout mice, and induction of RANTES was also observed in normal pregnant mice infected with the wild type, but not in those infected with the intracellular replication-defective mutant. (elsevierpure.com)

Previous reports of Brucella in amphibians have also included asymptomatic infections, suggesting that Brucella may be a commensal microorganism or opportunistic pathogen ( 9 ). (cdc.gov)
[ 7 ] Surprisingly, infection with Brucella species accounts for as many as 10% of laboratory-acquired infections, 24% of laboratory-acquired bacterial infections, and 11% of occupational-exposure deaths in the United States. (medscape.com)
Yoon-Suk created a versatile bioreporter toolkit to enable analysis of the fate of individual pathogens in polymicrobial infections. (kirbylab.org)
As a facultative intracellular food-borne pathogen, it is responsible for both severe central nervous system and fetal infections in humans and in a large variety of animals [ 5 ]. (biomedcentral.com)

The innate immune system plays a crucial role in host defense against invading pathogens and relies on pattern recognition receptors (PRRs), which detect conserved microbial- or danger-associated molecular patterns (MAMPs or DAMPs). (hindawi.com)
The innate immune response against B. abortus infection begins with the recognition of molecular structures related to this pathogen by receptors such as Toll-like receptors (TLRs) [ 15 ]. (hindawi.com)
The phagocytosis and destruction of pathogens in lysosomes constitute central elements of innate immune defense. (nsf.gov)
Due to the long-term exposure to pathogens, the mammalian innate immune system has evolved a faithful mechanism to rapidly sense and respond to Pathogen-Associated Molecular Patterns (PAMPs) based on their pattern recognition receptors (PRRs) [ 1 ]. (biomedcentral.com)

Brucella abortus and Listeria monocytogenes were used as the bacterial antigen in this study. (biomedcentral.com)

We observed a downregulation in the expression of genes involved in host response, such as TNF signaling, IL-1β production, and phagosome formation in Mφ, and phosphatidylinositol signaling and TNF signaling in PMNs, being in line with the ability of the pathogen to survive within phagocytes. (frontiersin.org)
Transmission may be horizontal and vertical, and as Brucella species have been isolated from lungworm and can survive in fish, ingestion may also be an important mode of transmission. (msdvetmanual.com)

Brucella abortus and Pregnancy in Mice: Impact of Chronic Infection on Fertility and the Role of Regulatory T Cells in Tissue Colonization. (harvard.edu)

From a comparative medical perspective, Bartonella are critically important emerging pathogens in both human and veterinary medicine. (ncsu.edu)

The first example of this was Rhodobacter sphaeroides in 1989, but additional discoveries quickly followed with Brucella melitensis in 1993, Burkholderia cepacia complex in 1994, Rhizobium meliloti in 1995, Bacillus thuringiensis in 1996, and now about 10% of bacterial species are known to have large replicons that are separate from the main chromosome. (wikipedia.org)

For example, Leal-Klevezas and colleagues stated that detectable amounts of antibodies are not recorded in the first 12-16 days after artificial inoculation of goats with Brucella abortus [ 19 ]. (biomedcentral.com)

2002. The genome sequence of the facultative intracellular pathogen Brucella melitensis . (mgc.ac.cn)
The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. (ecu.edu)

Examples of these pathogens include Chlamydiae, Chlamydophila species, and rickettsiae. (msdmanuals.com)

Using commercially available rapid bacterial identification systems such as the API 20 NE ® (BioMerieux, Nürtingen, Germany) which include a restricted number of biochemical tests Brucella spp. (biomedcentral.com)

We describe the isolation of atypical Brucella inopinata -like species and unique clinicopathologic findings in 2 adult marine toads ( Rhinella marina ), including oophoritis in 1 toad. (cdc.gov)
We report atypical Brucella infection in 2 marine toads. (cdc.gov)
The biotyping system developed for the identification of Brucella and differentiation of its species and biovars may replace or at least complement time-consuming tube testing especially in case of atypical strains. (biomedcentral.com)
A biotyping assay useful for Brucella identification and species differentiation must consequently be able to identify the rising number of upcoming new species as well as single atypical strains which do not fit within the pre-existing scheme [ 10 , 11 ]. (biomedcentral.com)

The immune response against Brucella infection involves many molecules and cells to trigger a Th1 immune response and activation of CD8+ T cells [ 12 - 14 ]. (hindawi.com)
May 13, 2021 B cells are the immune cells responsible for creating antibodies, and most produce antibodies in response to a pathogen or a vaccine. (sciencedaily.com)
Lymph nodes are useful samples for the assessment of host immune responses but can also provide a source of pathogen RNA for study, such as the case of intra-cellular pathogens present in immune cells inside lymph nodes. (jove.com)
IMPORTANCE The immune system must be able to tailor its response to different types of pathogens in order to eliminate them and protect the host. (biomed.news)
Our work establishes BLOS1 as a novel immune defense factor whose activity is hijacked by diverse pathogens. (nsf.gov)

Two new brucella species, provisionally called B. pinnipediae and B. cetaceae , have been isolated from marine hosts within the past few years. (mgc.ac.cn)

Brucella: Molecular Microbiology and Genomics Caister Academic Press. (unamur.be)
Little is known about the molecular basis of Brucella adherence to host cells. (ntu.ac.uk)
Brucella a bortu s ornithine lipids are dispensable outer membrane components devoid of a marked pathogen-associated molecular pattern. (bio-protocol.org)
However, the precise molecular mechanisms of the vertical transmission of these pathogens are still unclear. (biomedcentral.com)

The effects of 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB-126), which is the most toxic congener of coplanar polychlorinated biphenyls (Co-PCBs), on intracellular accumulation and transepithelial transport of vinblastine were examined in porcine kidney cells, LLC-PK1, and its transformant cells expressing human P-glycoprotein (LLC-MDR1). (go.jp)
Conclusions: Our results highlight the possible role of Bp26 protein in the adhesion process of Brucella to host cells through ECM components. (ntu.ac.uk)
B. melitensis cells have a surface smooth (S)-type lipopolysaccharide (LPS) made of a lipid A-core oligosaccharide linked to an N-formylperosamine O-polysaccharide (O-PS) that carries the epitopes relevant in S Brucella serodiagnostic tests [ 3 ]. (biomedcentral.com)
However, little is known about phagocytic functions of TG cells against the pathogens. (biomedcentral.com)

Brucella abortus virulent strain S2308 was obtained from our laboratory collection [ 22 ]. (hindawi.com)
The strain S2308 was grown in Brucella Broth liquid medium (BB) (DIFCO) at 37°C under constant agitation. (hindawi.com)
Brucella species and biovars revealed characteristic metabolic profiles and each strain showed an individual pattern. (biomedcentral.com)

These findings represent a novel emerging disease in toads and a possible zoonotic pathogen. (cdc.gov)
A healthy person lives in harmony with the microbial flora that helps protect its host from invasion by pathogens, usually defined as microorganisms that have the capacity to cause disease. (msdmanuals.com)
Pinnipeds have less Brucella -associated pathological changes by comparison, although B pinnipedialis has been associated with various organs and disease processes. (msdvetmanual.com)

Since the 1990s, previously unknown strains of Brucella have been found in both captive and free-ranging pinnipeds and cetaceans from many countries. (msdvetmanual.com)

The ability of Brucella protein Bp26 to bind to extracellular matrix (ECM) proteins was determined by enzyme-linked immunosorbent assay (ELISA) and biolayer interferometry (BLI). (ntu.ac.uk)

Anatomy3

Organisms18

Diseases4

Chemicals and Drugs7

Phenomena and Processes4

Information Science1

Brucellosis18

Macrophages11

Bacteria12

Genus6

Facultative intracellular pathogens3

Virulence5

Pathogenic4

Bacterium3

Organisms2

Salmonella1

Coccobacilli4

Pathogenesis1

Suis4

Biovar1

Isolates3

Abortion3

Type IV secretion1

Important pathogen2

Obligate2

Rickettsia1

Ceti3

Aerobic1

Chlamydia1

Helicobacter1

Mycobacterium1

Replication-defective mutant1

Infections4

Innate4

Listeria1

Survive2

Mice1

Bartonella1

Burkholderia1

Goats1

Genome sequence2

Chlamydophila1

Biochemical1

Atypical4

Immune5

Hosts1

Molecular4

Cells4

Strain3

Disease3

Strains1

Extracellular1