Intracellular filamentous actin. Medical search. Frequent questions

In addition, 1-deoxysphinganine altered cytoskeleton dynamics, resulting in intracellular accumulation of filamentous actin and activation of the RhoGTPase Rac1. (uzh.ch)
Abl tyrosine kinases likely play important roles as downstream receptors in regulating actin cytoskeleton remodeling and intracellular trafficking. (asn-online.org)
In human microVSM, increasing intracellular cAMP by direct activation of adenylyl cyclase by forskolin (10 μM) or selectively activating Epac-Rap signaling by the cAMP analog 8-pCPT-2′-O-Me-cAMP (100 μM) activated RhoA, increased α 2C -AR expression, and reorganized the actin cytoskeleton, increasing F-actin. (johnshopkins.edu)
F-Actin stains are commonly used to characterize the stucture and functionality of the cytoskeleton. (aatbio.com)
Because the actin cytoskeleton is a very dynamic and labile structure in living cells it typically requires either cold methanol or paraformaldehyde fixation prior to labeling actin. (aatbio.com)
Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. (tebubio.com)
2014). Our data indicate that Tat activates a RhoA/ROCK pathway leading to remodeling of the actin cytoskeleton resulting in reduced NMDA-evoked responses. (cancer8.info)
Proteins also have structural or mechanical functions, such as actin and myosin in muscle and the proteins in the cytoskeleton, which form a system of scaffolding that maintains cell shape. (transectscience.org)
Pereyra, E. Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton. (uba.ar)
Moreno, S. 'Mucor rouxii ultrastructure: Cyclic AMP and actin cytoskeleton' (2006) Protoplasma. (uba.ar)
Actin cytoskeleton inside the outermost cytoplasmic layer is interrupted following CNC injury Fluorescently labeled phalloidin toxin binds to and labels filamentous-actin inside the cell cytoskeleton.14 As Cajal bands are largely comprised of a network of filamentous actin, we assessed morphological alterations in microstructure along the length of teased nerve fibers by staining with phalloidin-FITC (Figure 6, left). (scdinhibitor.com)
Studies in 2D cell monolayers have shown that several species of bacteria usurp the host actin cytoskeleton to propel themselves into neighbouring cells either via lateral cell membranes or via filopodia extensions. (biologists.com)
Induction of the inflammatory or heat-shock responses caused subtle and distinct rearrangement of the actin cytoskeleton. (usuhs.edu)
Sequential stresses that mimic pathophysiologic 'two-hit' stimuli induce a characteristic fractal rearrangement of the actin cytoskeleton. (usuhs.edu)

Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in fixed cells and tissues, as well as, for visualizing individual actin filaments in vitro . (aatbio.com)
Interactions between F-actin and regulatory proteins such as actin-binding proteins rapidly assemble and disassemble actin filaments organizing them into actin bundles and cytoskeletal networks. (aatbio.com)
Once bound to F-actin, phalloidin shifts the equilibrium of monomers and filaments toward the filaments position, and inhibits ATP-hydrolysis. (aatbio.com)
The interaction stabilizes actin filaments by preventing subunit dissociation, and it promotes actin polymerization by lowering the critical concentration. (aatbio.com)
Unlike some antibodies against actin, phalloidin's capacity to bind more tightly to actin filaments over monomers decreases non-specific staining and background noise, producing better contrast between stained and unstained areas. (aatbio.com)
Due to its notable staining abilities, an array of phalloidin derivatives has been developed to study and visualize actin filaments and actin-related structures. (aatbio.com)
The computer-aided homology modelling revealed that Rg1 preferentially positioned in the actin binding cleft of myosin IIA and might block the binding of myosin IIA to actin filaments. (ijbs.com)
Interestingly, staging the infection cycle in worms by pulse-chase experiments revealed the presence of short and long intracellular bacterial filaments at 16- and 24-hours post-infection (hpi) whereas by 38 and 48 hpi most worms contained only the coccobacilli form. (biologists.com)
Actin filaments are instrumental in driving processes such as migration, cytokinesis and endocytosis and provide cells with mechanical support. (biologists.com)
Besides, simultaneous VAEM tracking of cytoskeletal dynamics provided more insights into coordinated responses of actin filaments and microtubules. (biomedcentral.com)

Figure 2(c) illustrates a culture of Indian Muntjac cells that were labeled with phalloidin conjugated to Alexa Fluor 488 for the intracellular actin cytoskeletal network. (microscopyu.com)
Note the absence of signals from the orange (Alexa Fluor 555) and far-red fluorophores, but the presence of intense green fluorescence from several of the more prominent actin cytoskeletal elements. (microscopyu.com)
In addition, the cells were simultaneously probed for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 488 conjugated to phalloidin. (fsu.edu)
Studies recommended that mechanosensitive channels could possibly be tethered to cytoskeletal and external anchors via intracellular and extracellular linkers. (lckinhibitor.com)
Disruption of cytoskeletal elements (microfilaments or microtubules), or cell-matrix adhesions nNOS manufacturer inhibits or eliminates the mechanical force-induced enhance of intracellular calcium in endothelial cells (5). (lckinhibitor.com)
Therefore, mechanical forces transduced for the ion channel by way of cell adhesions along with the cytoskeletal network can have an effect on ion conductivity and activate intracellular signaling in an amplitudedependent style. (lckinhibitor.com)
As a consequence, these characteristics have made phalloidin derivatives useful stains in visualizing F-actin and cytoskeletal networks. (aatbio.com)
a) Actin disruption is the cytoskeletal component most frequently investigated by studies. (pepas.org)
cortactin a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. (affbiotech.cn)
These findings put some insights into the unique neuroprotective properties of Rg1 associated with the regulation of myosin IIA-actin cytoskeletal structure under oxidative stress and provide experimental evidence for Rg1 in CNS diseases. (ijbs.com)
Furthermore, the coupling of signal perception with cytoskeletal structures and intracellular second messengers also necessarily involves transduction across the PM. Therefore, the functional dissection of underlying cellular events in the vicinity of the PM, utilizing state-of-the-art techniques, is a priority. (biomedcentral.com)

Actin is a highly conserved family of proteins that form microfilaments. (aatbio.com)
BK001, the actin binding protein spin-down assay kit provides G- or F-actin (rabbit skeletal muscle origin) plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. (tebubio.com)
The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. (scriptiebank.be)
Comparative genome analysis suggests genes that play a role in the construction or functioning of large cable bacteria cells: the genomes of Ca . Electrothrix gigas encode a novel actin-like protein as well as a species-specific gene cluster encoding four putative pilin proteins and a putative type II secretion platform protein, which are not present in other cable bacteria. (uibk.ac.at)
This actin-like protein (denoted big bacteria protein, Bbp) may have a function analogous to other actin proteins in cell structure or intracellular transport. (uibk.ac.at)
In analogy to other actin proteins, Bbp could fulfill a structural role in the cell or potentially facilitate intracellular transport. (uibk.ac.at)
Proteins of the Sla2p/Hip1R family bind to actin and clathrin at endocytic sites in yeast and mammals. (rupress.org)
These vesicles contain the mannose 6-phosphate receptor involved in targeting proteins to the lysosome, and the actin nucleating Arp2/3 complex. (rupress.org)
Combining immunopurification and subsequent analytical mass spectrometry, hundreds of proteins, including synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in intracellular and extracellular signaling and a large variety of adhesion molecules, were identified. (mdpi.com)

Intriguingly, LRP6 loss caused cell morphological aberration, F-actin dynamics disruption, YAP nucleoplasmic relocation, and subsequent YAP inactivation. (biomedcentral.com)
Functionally, tumor cells incubated with LGRFYAASG-pen showed disruption of filamentous actin, focal adhesions and caveolae-mediated membrane trafficking, resulting in impaired cell adhesion and migration in vitro. (unm.edu)
Of the studies surveyed in this review, the majority focus on actin and MTs, with few studies considering disruption to IFs and 6-Carboxyfluorescein no studies considering disruption to septin (Physique 1a). (pepas.org)
Silencing of Hip1R expression by RNAi resulted in disruption of Golgi organization and accumulation of F-actin structures associated with CCVs on the TGN. (rupress.org)

Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. (wikipedia.org)
Moreover, this work uncovers a new peptide motif that binds to and inhibits intracellular annexin A2 as a candidate therapeutic lead for potential translation into clinical applications. (unm.edu)

The α 2C -ARs mobilized from the perinuclear region to intracellular filamentous structures and to the plasma membrane. (johnshopkins.edu)
These higher-order structures provide the mechanical and structural support essential for a multitude of cellular processes including intracellular transport, cytokinesis, cell motility, polarity and cell shape, gene regulation and signal transduction. (aatbio.com)
Because actin is essential in so many biological processes, tools (actin-specific antibody and phalloidin derived stains) for the visualization of these actin structures are essential in research. (aatbio.com)
In fixed cells, actin structures can be visualized by actin antibodies, fluorescent phalloidin, or electron microscopy. (aatbio.com)
Live imaging identifies dynamic and transient F-actin-based structures, such as filopodia, contractile ring and cell cortex, and more persistent F-actin-based structures, such as cell junctions. (biologists.com)

The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 568 conjugated to phalloidin, and for DNA with 4',6-diamidino-2-phenylindole ( DAPI ). (fsu.edu)

Moreover, disrupted F-actin dynamics inhibited osteogenic differentiation, proliferation, YAP nuclear translocation, and YAP activation in mechanically induced PDLSCs. (biomedcentral.com)
Similar results were obtained in murine wild-type microVSM, coupling Rap1-Rho-actin dynamics to receptor relocalization. (johnshopkins.edu)
To investigate the structural organisation, dynamics and functional importance of F-actin in endothelial cells (ECs) during angiogenesis in vivo , we generated a transgenic zebrafish line expressing Lifeact-EGFP in ECs. (biologists.com)

Western blotting, qRT-PCR, and phalloidin staining were adopted to affirm filamentous actin (F-actin) alteration. (biomedcentral.com)
Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. (aatbio.com)
Correctly designed fluorescent phalloidin only binding to the native quaternary structure of F-actin and therefore have a low background. (aatbio.com)
After fixation in 4% formaldehyde, cells were stained with F-actin probe Phalloidin-iFluor™ 633 Phalloidin (Cat No. 23125 ) and nuclear counterstain Nuclear Blue™ DCS1 (Cat No. 17548 ). (aatbio.com)
Compared to actin antibodies, non-specific binding of phalloidin is negligible, thus providing minimal background and high-contrast during cellular imaging. (aatbio.com)
Phalloidin derivatives are water soluble and when used at nanomolar concentrations can selectively stain F-actin. (aatbio.com)

Intracellular targeting of annexin A2 inhibits tumor cell adhesion, migration, and in vivo grafting. (unm.edu)
Accordingly, the neuroprotective mechanism of Rg1 is related to the activity that inhibits myosin IIA-actin interaction and the caspase-3/ROCK1/MLC signaling pathway. (ijbs.com)

Actins are highly conserved within the eukaryotic kingdom and exist in higher eukaryotes as multigene families. (tebubio.com)

The Rg1 treatment also abolished H 2 O 2 -induced morphological changes, including cell rounding, membrane blebbing, neurite retraction and nuclei condensation, which were generated by myosin IIA-actin interaction. (ijbs.com)

The bidirectional control of AQP2 trafficking from cytoplasm to the apical plasma membrane is regulated by vasopressin/vasopressin receptor (VP/VPR) pathway and filamentous actin (F-actin) polymerization/depolymerization. (asn-online.org)
BK003, The Actin Polymerization Biochem Kit is based on the enhanced fluorescence of pyrene conjugated actin that occurs during polymerization. (tebubio.com)
Drugs that prevent changes to filamentous actin blocked adaptation of NMDAR function following Tat-induced potentiation while stimulating either depolymerization or polymerization of actin attenuated NMDAR function. (cancer8.info)
Other cells were exposed to cytochalasin D, a fungal metabolite, which interferes with polymerization of actin from its globular to its filamentous form, and similarly were analyzed with respect to fractal dimension, viability (neutral red assay), and manner of death (annexin V fluorescence-activated cell scanning analysis). (usuhs.edu)

The integrins therefore serve to hyperlink across the plasma membrane two networks: the extracellular ECM as well as the intracellular actin filamentous technique by means of multiprotein focal adhesion complexes. (lckinhibitor.com)

Note the prominent staining of the intracellular microtubule network that extends throughout the cytoplasm. (microscopyu.com)
Using fluorescent dyes that mark the cytoplasm of intestinal cells and actin markers that define the apical and basolateral cell edges, the authors confirm that Bordetella atropi is indeed an intracellular pathogen. (biologists.com)

Filamentous cable bacteria also display substantial size differences, with filament diameters ranging from 0.4 to 8 µm. (uibk.ac.at)

For functional analysis, we used low concentrations of Latrunculin B that preferentially inhibited F-actin polymerisation in filopodia. (biologists.com)

Actin possesses a unique ability to dynamically polymerize into filamentous actin (F-actin) from its monomeric state. (aatbio.com)
It possesses a high binding affinity for the grooves between F-actin subunits over monomeric G-actin. (aatbio.com)

It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. (tebubio.com)

Immunofluorescence microscopy has shown that the area of host cell in proximity to the bacterium contains polymerized actin, α-actinin, talin, and ezrin ( 2 ). (cdc.gov)

Inhibition of intracellular 1-deoxysphinganine trafficking and ceramide synthesis improved the viability of the cells, indicating that the intracellular metabolites of 1-deoxysphinganine contribute to its cytotoxicity. (uzh.ch)

Intracellular pathogens are also detected via nucleotide-binding oligomerization domain-like receptors (NLRs), a class of PRRs that reside in the cytosol ( 28 ). (frontiersin.org)

The images presented in Figure 2 demonstrate the performance of this filter combination with a variety of blue-absorbing fluorescence probes targeted at different intracellular locations. (microscopyu.com)

VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. (wikipedia.org)

Due to its intracellular abundance in eukaryotic cells, well within micromolar concentrations, actin participates in the most protein-protein interactions than any other known protein. (aatbio.com)

These findings indicate that Tat activates a RhoA/ROCK signaling pathway resulting in actin remodeling GW4064 and subsequent reduction of NMDAR function. (cancer8.info)

These studies establish roles for Hip1R and actin in CCV budding from the TGN for lysosome biogenesis. (rupress.org)

Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches. (affbiotech.cn)

VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. (wikipedia.org)

By affinity purification and mass spectrometry, intracellular annexin A2 was identified as the corresponding binding protein. (unm.edu)
Actin binding occurs when there is an affinity for any site of actin. (tebubio.com)

In diverse species, actin assembly facilitates clathrin-coated vesicle (CCV) formation during endocytosis. (rupress.org)

The authors discover a bacterial pathogen in the nematode Oscheius tipulae that adopts a filamentous form to spread across host intestinal cells. (biologists.com)

We identified that LRP6 in PDLSCs acted as the mechanosensor regulating mechanical stress-inducible osteogenic commitment via the F-actin/YAP cascade. (biomedcentral.com)

Intracellular signaling by the second messenger cyclic AMP (cAMP) activates the Ras-related small GTPase Rap1 through the guanine exchange factor Epac. (johnshopkins.edu)

Activation of RhoA/ROCK signaling increases the formation of filamentous actin. (cancer8.info)

The different actin isoforms can show distinct cellular and sub-cellular expression and localization. (tebubio.com)

Together, our findings demonstrate an unrecognized functional link between intracellular annexin A2 and tumor cell adhesion, migration and in vivo grafting. (unm.edu)

Antibodies recognize both monomer and polymer (filamentous or F-actin) actin and hence tend to have a high background compared to probes that bind only F-actin. (aatbio.com)

The novel actin-like protein was also found in a number of other giant bacteria, suggesting there could be a genetic basis for large cell size. (uibk.ac.at)

We hypothesized that Hip1R might also coordinate actin assembly with clathrin budding at the trans-Golgi network (TGN). (rupress.org)

However, mechanistic details are lacking, and recent studies have implicated ALMS1 in several processes including endosomal trafficking, actin organisation, maintenance of centrosome cohesion and transcription. (springer.com)

During angiogenesis, actin-rich filopodia protrusions have been proposed to drive endothelial tip cell functions by translating guidance cues into directional migration and mediating new contacts during anastomosis. (biologists.com)

Tubulin, Actin, and DNA Distribution in 3T3 Cells - A culture of 3T3 cells was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to the cyanine dye, Cy3. (fsu.edu)

Indeed, our insights into force-instigated intracellular signaling cascades that regulate osteogenic activities in PDLSCs have gained momentum [ 9 ]. (biomedcentral.com)

This kit allows you to quantify glutamine present in culture medium or intracellular glutamine by WST reduction reaction. (tebubio.com)

Anatomy31

Organisms9

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes31

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Cytoskeleton14

Filaments11

Cytoskeletal11

Proteins9

Disruption4

Binds2

Structures5

Alexa Fluor1

Dynamics3

Phalloidin6

Inhibits2

Highly conserved1

Myosin1

Polymerization4

Focal1

Cytoplasm2

Filament1

Polymerisation1

Monomeric2

Cytokinesis1

Talin1

Inhibition1

Receptors1

Fluorescence1

Extracellular1

Eukaryotic1

Pathway1

Roles1

Arp21

Motility1

Affinity2

Facilitates1

Bacterial1

Mechanical1

Cyclic1

Activation1

Cellular1

Vivo1

Probes1

Protein1

Golgi1

Processes1

Migration1

Cells1

Insights1

Reduction1