Intracellular clara cell. Medical search. Frequent questions

To study the possible effect of E2 on oxidative stress response, RCC cells were stained with 2,7-dichlorofluorescein diacetate and analyzed by flow cytometry. (biomedcentral.com)
On the other hand, the level of reactive oxidative species were elevated in ACHN cells after E2 treatment. (biomedcentral.com)
Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise autophagy. (biomedcentral.com)
However, effects of TFEB-mediated autophagy on the oxidative stress of bovine mammary epithelial cells remain unknown. (biomedcentral.com)
Therefore, the main aim of the study was to investigate the role of TFEB-mediated autophagy in bovine mammary epithelial cells experiencing oxidative stress. (biomedcentral.com)
Because apoptosis of bovine mammary epithelial cells is modulated by cellular redox state [ 11 ], developing potential strategies for maintaining redox balance is particularly important in terms of preventing oxidative damage. (biomedcentral.com)
Thus, TFEB-mediated autophagy in bovine mammary epithelial cells might play a positive role in response to oxidative stress. (biomedcentral.com)
Moreover, momordicine I decreased the oxidative phosphorylation capacity of glioma cells and inhibited tumor sphere formation in temozolomide (TMZ)-resistant GBM cells. (excli.de)
Dr. Franco was the first to demonstrate that tyrosine nitration, an oxidative modification to proteins that occurs in pathological and inflammatory conditions, regulates key processes of tumor cell metabolism. (health.mil)
Besides, Caco-2 and HepG2 cells allowed the study of intracellular oxidative stress assessed in both apical and basolateral domains. (bvsalud.org)

During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
Moreover, cancerous cells tend to cluster together when they are detached from the extracellular matrix. (elifesciences.org)
Moreover, PVRL4 triggers a form of signaling between the cells called integrin β4 signaling that allows them to survive without being anchored to the extracellular matrix. (elifesciences.org)
IsoPlexis has also developed the only technology able to phenotype each immune cell by its extracellular function. (paralab-bio.pt)
We have shown previously that extracellular psoriasin binds to the receptor for advanced glycation end products (RAGE), promoting endothelial cell proliferation (8). (medicaljournals.se)
Previous studies of the role of psoriasin in keratinocyte differentiation have focused on the effects of extracellular psoriasin, but as the psoriasin in psoriasis is keratinocyte-derived, it is imperative to consider the effects of psoriasin as an endogenously produced intracellular mediator. (medicaljournals.se)
Here, we use two animal models and several techniques (two-photon imaging of CBF and neuronal calcium dynamics, intracellular and extracellular recordings, local pharmacology) to analyze the relationship between neuronal activity and local CBF during odor stimulation in the rodent olfactory bulb. (jneurosci.org)
Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
Similarly, reprogramming of somatic cells involves a complex interaction among intracellular and extracellular signals leading to epigenetic remodeling [6]. (unicyte.ch)

In this study, we demonstrated the cellular effects of E2 on DNA repair, ROS production as well as Nrf2 activation, and apoptosis in RCC cell lines. (biomedcentral.com)
Inhibition of autophagy via bafilomycin A1 aggravated H 2 O 2 -induced reactive oxygen species (ROS) accumulation and apoptosis in MAC-T cells. (biomedcentral.com)
Momordicine I also induced apoptosis, intracellular reactive oxygen species (ROS) production, and senescence in glioma cells. (excli.de)

These data demonstrate that African green monkey-like natural killer cell differentiation profiles can be rescued in rhesus macaques to promote viral clearance in tissues. (nature.com)
define NK cell differentiation states based on their education via NKG2a and expression of CD16 (FcγRIII), an activating Fc receptor that mediates antibody-dependent cell-mediated cytotoxicity (ADCC) 8 , and demonstrate that nonpathogenic SIVagm infection in African green monkeys (AGMs) imprints the maturation of NK cells inducing terminally differentiated NKG2a low CD16 + NK cells, which express high levels of interleukin (IL)−21R. (nature.com)
In psoriasis, the differentiation process of the epidermal cells is disturbed. (medicaljournals.se)
In a spectrum of conditions of dysregulated keratinocyte differentiation, we have previously demonstrated strong psoriasin expression in differentiated squamous cell carcinoma and a lack of expression in undifferentiated basalioma (9). (medicaljournals.se)
The aim of this study was therefore to investigate the effect of keratinocyte-derived, intracellular psoriasin on keratinocyte differentiation. (medicaljournals.se)
Gene targets of the altered miRNAs in all the ASD subgroups were enriched in pathways of neuronal development and synaptic plasticity, along with cell proliferation/differentiation. (frontiersin.org)
Several other environmental factors including oxy- gen concentration and mechanical, metabolic, and bio- chemical conditions have been shown relevant in cell differentiation and have been reviewed extensively (Fig. 1) [3]. (unicyte.ch)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

In SIVagm infection, systemic IL-15 was associated with NK cell proliferation in lymph node (LN), while systemic IFNα correlated with NK cell cytotoxicity in LN 17 . (nature.com)
Using human and mouse cell culture models of NF2 in combination with intracellular cell signaling arrays and metabolic profiling, she proposes to determine the metabolic phenotype of NF2-associated schwannoma cells, then identify the signaling pathways that are regulated by tyrosine nitration and how they promote schwannoma cell proliferation and survival. (health.mil)
A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
The Notch pathway plays a key role in different types of cancer and drives several biological processes relevant in MM, including cell localization within the bone marrow, proliferation, survival and pharmacological resistance. (oncotarget.com)

These differentially expressed immune-related genes were deemed to have a role in the process of Plasmodium infection in the host via dendritic/T regulatory cells and the TGF-β/Smad 2/3 signaling pathway. (biomedcentral.com)
Furthermore, we also demonstrate that glutamatergic synaptic transmission and rises in dendritic intracellular calcium ([Ca 2+ ] i ) are required to trigger blood flow responses. (jneurosci.org)

Intracellular psoriasin has been found to interact with various signalling proteins, including the nuclear factor (NF)-kB regulator Jab1, while secreted psoriasin functions as an antimicrobial peptide and has the ability to induce chemotaxis of neutrophils and T cells (6). (medicaljournals.se)

The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)

Elevation of intracellular Clara cell 10-kDa protein in central acini of rats exposed for 20 months. (cdc.gov)
The regional restructuring of the central acinus in rats following long term exposure to ozone (10028156) was examined, and factors, including Clara cell 10 kilodalton protein (CC10), that may make some target cell populations more resistant to injury, were identified. (cdc.gov)
The SP-B gene is on human chromosome 2, and its primary translation product is 40 kd, which is clipped to become an 8-kd protein in the type II cells before entering lamellar bodies to be cosecreted with phospholipids. (medscape.com)
Effects of EGCG on mitogen-activated protein kinase (MAPK) phosphorylation were determined by cell-based enzyme-linked immunosorbent assay (ELISA) and western blotting. (molvis.org)
H 2 O 2 challenge of the bovine mammary epithelial cell MAC-T increased protein abundance of LC3-II, increased number of autophagosomes and autolysosomes while decreased protein abundance of p62. (biomedcentral.com)
Upon nutrient deprivation, autophagy is often up-regulated in response to metabolic stress and excessive organelle damage to facilitate aggregated protein removal and provide energy for cells [ 13 ]. (biomedcentral.com)
Dulhunty and her colleagues were studying how the ryanodine receptor, a type of protein receptor, functions in muscle cells. (the-scientist.com)
Three years after the initial discovery, they found that another protein structurally related to glutathione transferases, a chloride intracellular ion channel, CLIC-2, could also dampen the activity of the ryanodine receptor in the heart. (the-scientist.com)
They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)
IsoPlexis, a complete Single-Cell proteomics automated solution, is dedicated to monitor several protein pathways with results available on the same day thanks to the world's most precise, award-winning detection system. (paralab-bio.pt)
The Single-Cell Intracellular Proteome reveals individual protein function and also provides context to entire protein signalling pathways, uncovering how the network operates as a whole. (paralab-bio.pt)
Flavopiridol treatment notably upregulated the protein expression of LC3, Beclin-1 and Atg7 in infected corneas as well as in RAW 264.7 cells. (researchsquare.com)
This was for two reasons: (1) mice have a thin olfactory nerve layer compared to rats and this will favor drug access to the glomerular layer, and (2) transgenic G-CaMP2 mice express the fluorescent calcium sensor protein G-CaMP2 in mitral cells. (jneurosci.org)

These results further enhance current understanding of the interaction between Plasmodium and host cells. (biomedcentral.com)
2012 ). In vitro , flavopiridol suppresses the interaction between leukocytes and endothelial cells in the model of murine hepatitis, which is a crucial step in the initiation of inflammation (Schmerwitz et al. (researchsquare.com)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Application of 10 μmol/l L-783,281 for 40 s to isolated β-cells in the presence of 3 mmol/l glucose increased [Ca 2+ ] i to 178 ± 10% of basal levels ( n = 18) as measured by fluo-4 fluorescence. (diabetesjournals.org)
As a key substrate for generating biological energy in all of the body's cells, glucose is vital for sustaining life. (drrathresearch.org)
In all aspects, optimum intracellular transport of glucose is essential for a healthy metabolism and for maintaining healthy blood glucose levels. (drrathresearch.org)
The ensuing nanoprobes coated with simple sugar units (galactose or glucose) allowed assessing information about their intracellular localization (vesicular structures), with impressive sensitivity towards complex environments and proved the ability to overcome biological auto-fluorescence and high penetration in tissues. (ua.pt)
Labeling of Trypanosoma brucei cell cultures with 50% uniformly 13C-labeled glucose demonstrated incorporation of glucose-derived carbon into 187 of 588 putatively identified metabolites in diverse pathways including carbohydrate, nucleotide, lipid, and amino acid metabolism. (acs.org)

Although it would be useful to understand how neurons regulate intracellular cytosolic free Zn 2+ concentrations ([Zn 2+ ] i ), at present, the tools available for measuring dynamic changes in [Zn 2+ ] i are limited. (jneurosci.org)

Therefore, the aim of this study was to understand whether E2 might induce different cellular effects on RCC cells. (biomedcentral.com)
Membrane vesicles, actively released by cells, represent a mechanism of intercellular communication that is conserved evolutionarily and involves the transfer of molecules able to induce epigenetic changes in recipient cells. (unicyte.ch)

She demonstrated the role of Ca2+ ions as intracellular signals and on actin polymerization. (wikipedia.org)

Cellular assays combined with metabolomics highlight the dual face of phenolics: From high permeability to morphological cell damage. (bvsalud.org)
In vitro experiments showed that chenodeoxycholic acids could also disrupt cellular membranes in the alveoli, increasing cationic permeability and intracellular Ca 2+ concentration, leading to overloading of Ca 2+ in cells, and causing injury to type II pneumonocyte [ 13 ]. (hindawi.com)

L-783,281, an antidiabetic fungal metabolite that has previously been shown to activate insulin signaling in CHO cells, was tested for its effect on intracellular Ca 2+ ([Ca 2+ ] i ) and insulin secretion in single mouse pancreatic β-cells. (diabetesjournals.org)
L-783,281 also resulted in insulin secretion at single β-cells. (diabetesjournals.org)
Virtually no secretion was evoked by L-783,281 in IRS-1 -/- β-cells. (diabetesjournals.org)
Later studies have demonstrated secretion of psoriasin from epithelial cells (5). (medicaljournals.se)
Active Notch signaling in MM cells induces the secretion of the key osteoclastogenic factor, RANKL, which can be boosted in the presence of stromal cells. (oncotarget.com)

The bovine mammary epithelial cell undergoes serious metabolic challenges and excessive ROS production during the transition period. (biomedcentral.com)
IHC-P: Human lung and HEK-293T (human embryonic kidney epithelial cell) transfected with a SCGB1A1 expression vector containing a his tag tissues. (abcam.com)

immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

Trends in Cell Biology. (wikipedia.org)
Dr. Franco aims to apply her expertise in redox biology and cell metabolism to the field of NF type 2 (NF2). (health.mil)
She was introduced to the field by Dr. Cristina Fernandez-Valle, an expert in NF2 and Schwann cell biology and a close collaborator and mentor. (health.mil)
Completed his PhD in Cell Biology and Virology in 2012 by Imperial College London Faculty of Medicine, his MSc in Genetics and Molecular biology in 2008 by Universidade de Lisboa Faculdade de Ciências and his BSc in Microbiology and Genetics in 2006 by Universidade de Lisboa Faculdade de Ciências. (cienciavitae.pt)
Rather than having to wait sometimes days to get the results of a biochemistry experiment, with electrophysiology "you see what is happening in an individual cell in the moment," says the muscle biology researcher and now emeritus professor at Australian National University in Canberra. (the-scientist.com)
The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)

Together these cellular alterations may contribute to the reduced viability of RCC cells following E2 treatment. (biomedcentral.com)
Pourmand's lab had established the underlying method of sampling material from single cells while maintaining cell viability in 2014 and the more recent study represents a way to reliably do so at scale. (genomeweb.com)
We tested four major extracts of M. charantia and found that momordicine I reduced glioma cell viability without serious cytotoxic effects on astrocytes. (excli.de)
A Cell Counting Kit-8 assay was used to test cell viability. (researchsquare.com)

For example, ex vivo IL-21 treatment expanded CD16 + NK cells 9 , antagonized the IL-15-dependent expansion of resting NK cells 10 , and reverses hypo-responsiveness via the STAT1 and PI3K-AKT-FOXO1 pathways 11 . (nature.com)

Regarding cellular damage, NS and BC extracts, when acting on the basolateral cellular side, caused epithelial leakage and morphological shape cell damage on Caco-2 cells associated with ROS production . (bvsalud.org)

Journal of Muscle Research and Cell Motility. (wikipedia.org)
There, he has been mostly devoted to the study of Ca2+ and redox modulation/signalling impact on cancer cell motility. (cienciavitae.pt)

Inhibitors,Modulators,Libraries Here, the computationally predicted functional purpose for Stat3 in primitive, but not definitive, erythroid cell maturation is validated in vitro. (hdac-inhibitors.com)
Natural killer (NK) cells are "licensed" with functional competence following education with self-major histocompatibility complex (MHC) class I molecules 1 . (nature.com)
Functional PMN chemotaxis assays showed a significant increase of PMN migration ( p = 0.010) and intracellular ROS production significantly increased throughout time ( p = 0.028). (biomedcentral.com)
These systems detect the rare subsets of highly functional superpowered cells by measuring the true cellular function defined by the full range of cytokines, chemokines, and other proteins . (paralab-bio.pt)
All two systems leverage the same uniquely innovative applications in functional immune profiling, intracellular signalling omics, and high-plex automated immunoassays, accelerating therapeutics development. (paralab-bio.pt)
Information exchange between cells coordinates devel- opment and functional interplay in complex organisms. (unicyte.ch)

This increase is caused by several factors, including increased concentration and cellular abundance of CC10 in granules and an increased mass of nonciliated secretory cells. (cdc.gov)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Immunohistochemical analyses of brain tissue specimens of R. typhi DNA-positive and -negative specimens showed perivascular B-cell accumulation. (cdc.gov)
In the proof-of-concept study, Pourmand's group aspirated 22 cells within a breast cancer tissue slice and selectively captured the mRNA using Nvigen's proprietary antibody-coated magnetic nanoparticles. (genomeweb.com)
Epithelial tissue is one of the four major types of tissue found in animals, and is the only type of tissue that is able to form and maintain layers of cells that are just one cell thick. (elifesciences.org)
Finally, in vivo histopathological analysis on F. hepatica -parasitized liver tissue sections of sheep showed multifocal infiltration of inflammatory cells within liver parenchyma, and further fluorescence microscopy analyses confirmed NETs formation in vivo. (biomedcentral.com)

These vesicles can transfer signals capable of altering cell function and/or reprogramming targeted cells. (unicyte.ch)
The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)
This process involves the ability of cells to change pheno- type depending upon specific signals. (unicyte.ch)
This concept is based on the observation that vesicles may transfer different types of signals between cells [7, 8]. (unicyte.ch)

LY294002 (25 μmol/l) significantly reduced the effect of the insulin mimetic on β-cell exocytosis. (diabetesjournals.org)
Hundreds of genes were sequenced from each cell at each aspiration, and the genes sequenced showed high correlations from one sampling to the next, indicating that the small amount of mRNA withdrawn from each cell did significantly affect gene coverage. (genomeweb.com)
Choline kinase and phospholipase C were significantly overexpressed, and lysophospholipase 1, phospholipase A2, and phospholipase D were significantly underexpressed, in breast cancer cells compared with HMECs. (aacrjournals.org)

Libraries had been loaded on ALK3 Purity & Documentation Illumina cBot for cluster generation on the flow cell, and sequenced in 50 bp single-end mode at six-fold multiplex around the Illumina HiSeq2000 (Illumina). (hivinhibitor.com)

Subsequently, the effect of specific lncRNAs on the modulation of immune-related signaling pathways in malaria was determined by fluorescence-activated cell sorting, western blot and enzyme-linked immunosorbent assay. (biomedcentral.com)
Other above represented cell adhe sion pathways include things like integrins, adherens junctions, nectins, and tight junctions. (hdac-inhibitors.com)
Metabolic fluxes between membrane and water-soluble pool of choline-containing metabolites were assessed by exposing cells to [1,2- 13 C]choline for long and short periods of time to distinguish between catabolic and anabolic pathways in choline metabolism. (aacrjournals.org)

In this study, we show that the common antiparasitic drug, atovaquone, inhibits arbovirus replication through intracellular nucleotide depletion and can impair ZIKV infection in an ex vivo human placental explant model. (nyu.edu)

We employed the HEK expression system and a biosensor allowing us to simultaneously detect the changes in intracellular diacylglycerol (DAG) and Ca 2+ concentrations. (mdpi.com)
We analyzed the gene expression profiles of individual monocyte-derived brain macrophages responding to TBI using single-cell RNA sequencing. (biomedcentral.com)
However, the expression of a given polarization marker was no more likely than at random to predict simultaneous expression or repression of markers of another polarization program within the same cell, suggesting a lack of exclusivity in macrophage polarization states in vivo in TBI. (biomedcentral.com)
Single-cell gene expression profiling demonstrated that monocytic macrophages in TBI are not comprised of distinctly polarized subsets but are uniquely and broadly activated. (biomedcentral.com)
The expression levels of E2 receptors (ER1 and ER2) were determined in different RCC cell lines. (biomedcentral.com)
Variable expression of ER1 and ER2 were found in the RCC cell lines studied (786-O, A498, and ACHN), in which ACHN and A498 showed highest and lowest ER expression, respectively. (biomedcentral.com)
We further explored whether the antiglioma effect of momordicine I may be related to cell cycle modulation and DLGPA5 expression. (excli.de)
In human CD4 + T cells, overexpression of NFAT1 and NFAT3 enhanced and suppressed IL-2 expression, respectively. (aai.org)
NFAT3 downregulation in Jurkat cells using RNA interference technology augmented IL-2 expression, whereas a knockdown of NFAT1, NFAT2, and NFAT4 suppressed it. (aai.org)
Downregulation of NFAT3 expression in T cells is mediated by lower chromatin accessibility and enhancer activity in its promoter in comparison with aortic smooth muscle cells expressing endogenous NFAT3. (aai.org)
Introduction of TBX5 into CD4 + T cells enhanced the expression of NFAT3 and suppressed that of IL-2. (aai.org)
ICC/IF: HEK-293T cells transfected with a human Uteroglobin expression vector containing a myc-his tag. (abcam.com)
In RAW 264.7 cells, flavopiridol treatment inhibited IL-1β, IL-6 and TNF-a expression but promoted IL-10 expression. (researchsquare.com)
Here we present evidences that MM can efficiently drive osteoclastogenesis by contemporaneously activating Notch signaling on tumor cells and osteoclasts through the aberrant expression of Notch ligands belonging to the Jagged family. (oncotarget.com)
Finally, MM cells through Jagged ligands expression can also activate Notch signaling in pre-osteoclast by direct contact. (oncotarget.com)
We also found, in separate studies, that microRNA (miRNA) expression in PBMo and mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) differed in the IL-1ß/IL-10-based ASD subgroups. (frontiersin.org)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

lung Clara cells and nasal olfactory cells (Cruzan et al . (cdc.gov)
Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)

Cell metabolic activity was measured using the alamarBlue assay. (molvis.org)
Excellent and concentration from the total RNA was measured by an Agilent 2100 Bioanalyzer RNA Nano assay (Agilent Technologies, Santa Clara, CA, USA). (hivinhibitor.com)

Cortical bone-derived stem cells (CBSCs) are reportedly essential for osteogenic lineages, bone maintenance and repair. (biomedcentral.com)
The experiments, performed in mice, showed that a drug that's commonly used in Alzheimer's treatments can stimulate natural repair processes in stem cells from the pulp, known as odontoblasts . (labiotech.eu)

Our results indicate that the cytotoxic effect of momordicine I on glioma cells suggests its potential therapeutic application to GBM treatment. (excli.de)
We validate the use of sugars as an all-in-one vector (Raman reporter, conferring high stability, biocompatibility and affinity to glioma cells) as imaging agents using an impressive technique. (ua.pt)

In this study, differences in choline membrane metabolism were characterized in breast cancer cells and normal human mammary epithelial cells (HMECs) labeled with [1,2- 13 C]choline, using 1 H and 13 C magnetic resonance spectroscopy. (aacrjournals.org)
The aberrant choline phospholipid metabolism of breast cancer cells and its strong correlation with malignant progression has been observed in several studies. (aacrjournals.org)

Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)

Upon activation, a light-activated biotin tag is used to bind the poly-A tails of mRNA, capturing mRNA within live cells and intact tissues in a relatively noninvasive manner. (genomeweb.com)
Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)

We used the ratioable fluorescent dye mag-fura-5 to measure intracellular free Zn 2+ ([Zn 2+ ] i ) in cultured neocortical neurons exposed to neurotoxic concentrations of Zn 2+ in concert with depolarization or glutamate receptor activation and identified four routes of Zn 2+ entry. (jneurosci.org)

Al though it has been shown that EPO induces tyrosine phosphorylation of Stat3 in addition to a likely purpose for this gene continues to be inferred in fetal definitive erythropoi esis through pathway examination, activation of Stat3 is uncommon in hematopoietic cell lines. (hdac-inhibitors.com)

There was no effect of EGCG on cell metabolic activity at any of the doses tested (0.3-30 µM). (molvis.org)
It is critically important to understand the molecular causes underlying these metabolic differences, because this may identify novel targets for attack in cancer cells. (aacrjournals.org)
0.001) of breast cancer cells compared with HMECs, indicating a higher metabolic flux from membrane phosphatidylcholine to choline and phosphocholine in breast cancer cells. (aacrjournals.org)

The utility of longitudinal data in single-cell spatial transcriptomics is well known and numerous methods attempt to address it. (genomeweb.com)

Transcriptional integration of upstream signaling Examination of M6 unveiled an enrichment for TFs together with other nuclear proteins involved in cell cycle regulation, chromatin modifications, and epigenetic regulation. (hdac-inhibitors.com)

Here we report, using the pathogenic model of antiretroviral therapy-treated, SIV-infected rhesus macaques that sequential interleukin-21 and interferon alpha therapy generate terminally differentiated blood natural killer cells (NKG2a/c low CD16 + ) with potent human leukocyte antigen-E-restricted activity in response to SIV envelope peptides. (nature.com)
The frequency and activity of terminally differentiated NKG2a/c low CD16 + natural killer cells correlates with a reduction of replication-competent SIV in lymph node during antiretroviral therapy and time to viral rebound following analytical treatment interruption. (nature.com)

Ryanodine is an ion channel, embedded in an internal membrane within the muscle cell, that surrounds a pocket of calcium ions. (the-scientist.com)

In β-cells from IRS-1 -/- mice, 10 μmol/l L-783,281 had no significant effect on [Ca 2+ ] i ( n = 5). (diabetesjournals.org)

Transcriptome in vivo analysis (TIVA), for example, enters cell membranes via a cell-penetrating peptide. (genomeweb.com)

Once migrated to the bone marrow, MM cells unbalance bone formation and resorption via the recruitment and maturation of osteoclast precursors. (oncotarget.com)

However, if absorbed, the substance will tend to distribute predominantly into the intracellular compartment. (europa.eu)

The analysis of signature polarization genes by single-cell RNA sequencing revealed the presence of diverse activation states, including M(IL4), M(IL10), and M(LPS, IFNγ). (biomedcentral.com)

It is concluded that L-783,281 evokes [Ca 2+ ] i increases and exocytosis in β-cells via an IRS-1/PI3-K-dependent pathway and that the [Ca 2+ ] i increase involves release of Ca 2+ from intracellular stores. (diabetesjournals.org)

Given previous findings on a role of IL-21 and IFNα in regulating NK cell function, and that NKG2a low CD16 + NK cells are generated while expressing high levels of IL-21R in nonpathogenic SIV infection, we sought to determine whether immunotherapy with IL-21 and IFNα rescues AGM-like profiles of NK cell maturation and activity in SIV-infected rhesus macaques (RMs). (nature.com)

Ultrasound has been used as a modality to enhance the transfection of cells, purportedly via entry of genetic material through defects in the cell membrane produced by bubble cavitation events. (aus.edu)
Cells can communicate via physical interactions, in- cluding membrane bridge formation, such as tunneling nanotubes and cytonemes, and/or through the release of soluble factors [1-3]. (unicyte.ch)

This mechanism preserves the single-cell layer pattern throughout the body and prevents epithelial cells from growing in inappropriate locations. (elifesciences.org)

Over the past decade, one common feature consistently revealed by magnetic resonance (MR) spectroscopic studies is the elevation of phosphocholine (PC) and total choline-containing metabolites [tCho, glycerophosphocholine (GPC)+PC+free choline (Cho)] in cancer cells and solid tumors. (aacrjournals.org)

The kainate-induced increase was highly sensitive to Gd 3+ or Na + removal in most neurons but insensitive in a minority subpopulation ("cobalt-positive cells"), suggesting that a fourth route of neuronal Zn 2+ entry is through the Ca 2+ -permeable channels gated by certain subtypes of AMPA or kainate receptors. (jneurosci.org)

TBX5 deficiency-mediated downregulation of NFAT3 is crucial for the high cytokine-producing activity of T cells. (aai.org)

It's answering critical challenges by functionally defining each T cell, monocyte, and NK cell . (paralab-bio.pt)

Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)
used genetic techniques to silence PVRL4 in cells from breast tumors, they found that it reduced the formation of clusters by the cancer cells and also reduced their ability to grow in the absence of attachment. (elifesciences.org)

TBI macrophage activation in vivo is deeply complex, with individual cells concurrently adopting both inflammatory and reparative features with a lack of exclusivity. (biomedcentral.com)
furthermore, in vivo IFNα-induced NK cell cytotoxicity correlates with reductions in HIV-DNA during antiretroviral therapy (ART) 16 . (nature.com)

Anatomy25

Organisms10

Diseases15

Chemicals and Drugs52

Analytical, Diagnostic and Therapeutic Techniques and Equipment19

Phenomena and Processes20

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Health Care1

Geographicals2

Oxidative10

Extracellular14

Apoptosis3

Differentiation8

Proliferation4

Dendritic2

Signalling proteins1

Immunoglobulin1

Protein14

Interaction3

Glucose5

Cytosolic1

Induce2

Actin1

Permeability2

Secretion5

Epithelial cell2

Reactive1

Biology6

Viability4

Reverses1

Leakage1

Motility2

Functional6

Cellular2

Tissue4

Signals4

Significantly3

Cluster1

Pathways3

Inhibits1

Expression18

Lung2

Assay2

Stem cells2

Glioma cells2

Metabolism2

Phenotype2

Tissues3

Concentrations1

Gene1

Metabolic3

Transcriptomics1

Epigenetic1

Terminally differentiated2

Calcium1

Mice1

Membranes1

Bone marrow1

Substance1

Genes1

Involves1

Maturation1

Membrane2

Preserves1

Metabolites1

Neuronal1

Cytokine1

Monocyte1

Tumors2

Vivo2