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  • 2000
  • Because of the crucial role of the actin cytoskeleton in cytokinesis, many studies have been carried out to examine the distribution and behavior during cell division of actin itself and several actin-binding proteins such as myosin, profilin and cofilin ( Robinson and Spudich, 2000 ). (biologists.org)
  • genes
  • Specifically, these 16HBE14o- cells had increased levels of transcripts from genes associated with repair and inflammatory processes ( e.g. , matrix metalloproteinases, chemokines, and glutathione S-transferase). (biomedcentral.com)
  • There is an increased risk that the tumor cells will mutate other genes. (wikipedia.org)
  • cortical
  • Similarly, in dividing cells, a structure composed of Rho and Citron-K in cleavage furrow excludes cortical actin cytoskeleton, and disruption of F-actin disperses Citron-K throughout the cell cortex. (biologists.org)
  • mechanisms
  • Investigating the cellular and molecular signatures in eukaryotic cells following exposure to nanoparticles will further our understanding on the mechanisms mediating nanoparticle induced effects. (biomedcentral.com)
  • Here we studied the intracellular mechanisms by which 2-ME inhibits SMC growth and whether 2-ME prevents injury-induced neointima formation. (ahajournals.org)
  • In this investigation, we demonstrated the anticancer effect of SZC014, a novel OA derivative and identified the possible mechanisms by which SZC014 induced MCF-7 cell death. (sigmaaldrich.com)
  • susceptible
  • Crucially however, our bifurcation analysis reveals that stable fixed point and stable limit cycle solutions can coexist, and that calcium dependent Cyclin D dynamics extend the oscillatory region to lower Cyclin D synthesis rates, thus rendering cells more susceptible to cycling. (nottingham.ac.uk)
  • During certain phases of mitotic division cells are much more susceptible to damage. (washington.edu)
  • Second stage tumor promoters like mezerein do not have the capacity to initiate tumors, but can create circumstances in which initiated cells are more susceptible to additional mutations or in which initiated cells have growth advantage. (wikipedia.org)
  • vitro
  • However, significant challenges exist in the extrapolation of in vitro cell culture data to in vivo efficacy in animal models and to humans. (springer.com)
  • DNA replication can also be performed in vitro (artificially, outside a cell). (wikipedia.org)
  • budding yeast
  • No coat complexes are known to mediate transport directly from the trans -Golgi network (TGN) to the apical plasma membrane (PM) of polarized cells, which corresponds to secretory vesicles in the model organism Saccharomyces cerevisiae (budding yeast). (frontiersin.org)
  • induce
  • These results strongly suggest that Rho is activated during cell division and works as a switch to induce and maintain the cytokinetic apparatus. (biologists.org)
  • mRNA
  • Kim et al [ 10 ] reported that Ag + induced metal-responsive metallothionein 1b (MT1b) mRNA expression in AgNO 3 treated cells, but not in Ag-np treated cells. (biomedcentral.com)
  • tissues
  • Rapid commercialisation of these nanoparticles was boosted by the fallacy that they are less toxic to cells and tissues than other silver salts. (biomedcentral.com)
  • It is expressed mainly in brain tissues, white blood cells and the kidney. (wikipedia.org)
  • thus
  • Thus, there is a positive-feedback loop between the Lm cell division cycle and a physical interaction with the host cytoskeleton. (umass.edu)
  • Thus, the aim of this study was to determine the transcriptomic response of the human bronchial epithelial cell line (16HBE14o-) following interaction with A. fumigatus conidia. (biomedcentral.com)
  • cDNA
  • This is true whether Pur-alpha is microinjected or expressed after introducing a cloned PURA cDNA into cells. (wikipedia.org)
  • assays
  • Immunofluorescent staining and nystatin protection assays demonstrated that 16HBE14o- cells internalized 30-50% of bound conidia within six hrs of co-incubation. (biomedcentral.com)
  • surface
  • Instead, polymerization of host-derived actin at the bacterial cell surface extends the non-dividing elongation period and compresses the division period. (umass.edu)
  • The transport of Chs3 to the cell surface requires the exomer complex, which acts as a cargo adaptor for Chs3 and other cargos. (frontiersin.org)
  • Visit Fluidigm at Booth 79 and learn how you can deeply profile precious samples across a range of cell surface and intracellular markers, all from a single tube. (fluidigm.com)
  • gene
  • Expression and subcellular distribution of emerin were studied in lymphoblastoid cell lines established from four patients with Emery-Dreifuss muscular dystrophy containing different mutations in the emerin gene. (biologists.org)
  • Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression. (sigmaaldrich.com)
  • mechanism
  • To clarify further the mechanism of the Rho-mediated transfer and concentration of Citron-K in cleavage furrow, we expressed active Val14RhoA in interphase cells expressing GFP-Citron-K. Val14RhoA expression transferred Citron-K to the ventral cortex of interphase cells, where it formed band-like structures in a complex with Rho. (biologists.org)
  • human
  • Depletion of hRif1 by RNA interference in human cancer cells impaired cell growth but had no detectable effect on telomere length, although hRif1 overexpression in S. cerevisiae interfered with telomere length control, in a manner specifically dependent on the presence of yeast Rif1p. (rupress.org)
  • Total RNA was then isolated and the transcriptome of 16HBE14o- cells was evaluated using Agilent Whole Human Genome microarrays. (biomedcentral.com)
  • We demonstrate a methodology using FACs for analyzing the transcriptome of infected and uninfected cells from the same cell population that will provide a framework for future characterization of the specific interactions between pathogens such as A. fumigatus with human cells derived from individuals with or without underlying disease susceptibility. (biomedcentral.com)
  • Enable deep profiling of human T cell populations including expression of key checkpoint molecules. (fluidigm.com)
  • fibroblasts
  • TGF β was shown to transiently confer on normal fibroblasts phenotypic properties of transformed cells, as demonstrated by their acquired ability to grow in soft agar in an anchorage-independent manner [ 2 ]. (hindawi.com)
  • Mezerein has been shown to have two effects in chick embryo fibroblasts (CEF cells) that are associated with cancer. (wikipedia.org)
  • function
  • This data suggests that for emerin to function normally it must be correctly localized, retained at the nuclear membrane and phosphorylated by cell cycle-mediated events. (biologists.org)
  • CYTO ® 2017 features a number of talks and posters featuring mass cytometry as a key component in the discovery of new cell types and fresh insights into cellular function. (fluidigm.com)
  • It has a role in the proper function, maintenance, and formation of cilia which are found in all types of cells in the body. (wikipedia.org)
  • Amajor function of centrosomes in animal cells is to nucleate microtubules. (rupress.org)
  • actin
  • Host Actin Polymerization Tunes the Cell Division Cycle of an Intracellular Pathogen. (umass.edu)
  • By decreasing the relative proportion of dividing Lm, actin polymerization biases the population toward cells with the highest propensity to form actin tails. (umass.edu)
  • Disruption of F-actin abolished the band and dispersed the Citron-K-Rho-containing patches throughout the cell cortex. (biologists.org)
  • Rho is then activated, binds to Citron-K and translocates it to cell cortex, where the complex is then concentrated in the cleavage furrow by the action of actin cytoskeleton beneath the equator of dividing cells. (biologists.org)
  • bacteria
  • While they are well described for model organisms growing in broth culture, very little is known about the cell division cycle of bacteria replicating in more complex environments. (umass.edu)
  • For example, Pur-alpha is essential for brain and blood cell development in mammals, but bacteria have no brain and no blood. (wikipedia.org)
  • replication
  • The cell possesses the distinctive property of division, which makes replication of DNA essential. (wikipedia.org)
  • In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. (wikipedia.org)
  • cancer cells
  • Cancer cells in particular showed a concentration dependant increase in phosphorylated p53 accompanied by the cleavage of caspase 3 and PARP. (biomedcentral.com)
  • These data show that SZC014 is an effectively selective anticancer agent against breast cancer cells, highlighting the potential use of this derivative as a breast cancer therapeutic agent. (sigmaaldrich.com)
  • Furthermore, high doses of mezerein have been used to terminally differentiate cancer cells, preventing their growth. (wikipedia.org)
  • cleavage
  • After segregation of chromosomes to the opposite poles in anaphase, a cleavage furrow is formed around the equator of a dividing cell, which deepens in telophase finally to separate two daughter cells. (biologists.org)
  • In classical experiments using fertilized eggs of sea urchin or newt eggs, a ring composed of actomyosin is observed beneath the cleavage furrow and it is suggested that the constriction of this ring leads to the cleavage of the cell. (biologists.org)
  • phase
  • It has been hypothesised that cytoplasmic calcium accelerates entry into S phase of the cell cycle and/or acts to recruit otherwise quiescent cells onto the cell cycle. (nottingham.ac.uk)
  • 2-Methoxyestradiol (2-ME), an endogenous metabolite of estradiol with no affinity for estrogen receptors, is a potent anticarcinogenic agent (in phase II clinical trials) and mediates the inhibitory effects of estradiol on smooth muscle cell (SMC) growth. (ahajournals.org)
  • signatures
  • Using multiparameter mass cytometry, modular Maxpar® panel kits and a catalog of over 600 metal-labeled antibodies, you can reveal cell signatures in higher dimension to reach your next breakthrough. (fluidigm.com)
  • growth
  • Growth and division are two of the most fundamental capabilities of a bacterial cell. (umass.edu)
  • The transforming growth factor-beta (TGF β ) was discovered more than two decades ago and was isolated as a secreted factor from sarcoma virus-infected cells [ 1 - 3 ]. (hindawi.com)
  • This leads to growth advantage for initiated cells. (wikipedia.org)
  • found
  • Using a D-alanine reporter strategy, we found that intracellular Listeria monocytogenes (Lm) spend a smaller proportion of their cell cycle dividing compared to Lm growing in broth culture. (umass.edu)
  • binding protein
  • Background Cardiac stress may trigger production of the 40\kDa peptide fragment produced from the amino terminus from the cardiac myosin\binding protein C. function, cell viability, hypertrophy, or possibility of success. (biomasswars.com)
  • membrane
  • Their main structural uses are as part of biological membranes both internal and external, such as the cell membrane, or as a source of energy. (wikipedia.org)
  • Viroporins are capable of assembling into oligomeric ion channels or pores in the host cell's membrane, rendering it more permeable and thus facilitating the exit of virions from the cell. (wikipedia.org)
  • The most well-studied and well-established function of viroporins is the permeabilization of the cell membrane to ions and small solutes. (wikipedia.org)
  • Loss of membrane polarization can promote viral yields through a variety of mechanisms that operate throughout the viral life cycle. (wikipedia.org)
  • In non-enveloped viruses, the membrane permeability changes may be sufficient to induce cell lysis, thereby permitting the new virions to exit the cell. (wikipedia.org)
  • V-ATPase 116 kDa isoform a2 is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. (wikipedia.org)
  • The flagellum exits the cell anterior of nucleus and is connected to the cell body for part of its length by an undulating membrane. (wikipedia.org)
  • Another function of Hsc70 is as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. (wikipedia.org)
  • During a normal embryologic processes, or during cell injury (such as ischemia-reperfusion injury during heart attacks and strokes) or during developments and processes in cancer, an apoptotic cell undergoes structural changes including cell shrinkage, plasma membrane blebbing, nuclear condensation, and fragmentation of the DNA and nucleus. (wikipedia.org)
  • Industrially important impacts are on the maintenance of mariculture, membrane systems (e.g., membrane bioreactors and reverse osmosis spiral wound membranes) and cooling water cycles of large industrial equipment and power stations. (wikipedia.org)
  • vesicles
  • showed that HAP1 is localized in mitotic spindle of dividing striatal cells, and associated endosomes, microtubules and vesicles in the basal forebrain and striatial neurons - where HAP1B is preferentially expressed. (wikipedia.org)
  • They also make up the internal structure of cilia and flagella.They provide platforms for intracellular transport and are involved in a variety of cellular processes, including the movement of secretory vesicles, organelles, and intracellular macromolecular assemblies (see entries for dynein and kinesin). (wikipedia.org)
  • Enzymes
  • 2000 ). Because GadA and GadB are intracellular enzymes, and most MSG are out of the cells, the recombinant E. coli cells have to be specially treated by sonication, ethyl acetate, toluene, and thermal activation to increase the permeability (Zhao et al. (springeropen.com)
  • Enzymes also allow the regulation of metabolic pathways in response to changes in the cell's environment or to signals from other cells. (wikipedia.org)
  • reactions
  • Glycine-induced activation of strychnine-sensitive glycine receptors may also result in hyper- or depolarising reactions of the prospective cells with regards to the intracellular Cl? (biomasswars.com)
  • As these molecules are vital for life, metabolic reactions either focus on making these molecules during the construction of cells and tissues, or by breaking them down and using them as a source of energy, by their digestion. (wikipedia.org)
  • immune
  • Concanavalin A (ConA) has the ability to stimulate the activation of T lymphocytes, mostly CD4 + T-cells, leading to immune hepatitis in mice [ 3 , 4 ]. (hindawi.com)
  • Tumors of mice treated with PBT showed reductions in myeloid cells that suppress immune attack on tumor cells and significant increases in tumor-specific cytotoxic T cells. (wikipedia.org)
  • Rituximab is a chimeric monoclonal antibody against the protein CD20, which is primarily found on the surface of immune system B cells. (wikipedia.org)
  • Cardiac arrest Cytokine release syndrome Tumor lysis syndrome, causing acute renal failure Infections Hepatitis B reactivation Other viral infections Progressive multifocal leukoencephalopathy (PML) Immune toxicity, with depletion of B cells in 70% to 80% of lymphoma patients Pulmonary toxicity Bowel obstruction and perforation Two patients with systemic lupus erythematosus died of progressive multifocal leukoencephalopathy (PML) after being treated with rituximab. (wikipedia.org)
  • Citric
  • For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. (wikipedia.org)
  • They also code for citrate synthase and two subunits of 2-oxoacid:ferredoxin oxidoreductase, which plays the same role as alpha-ketoglutarate dehydrogenase in the TCA (tricarboxylic/Krebs/citric acid) cycle. (wikipedia.org)
  • Junctions
  • These complexes, which help regulate cell growth in addition to creating and maintaining epithelial layers, are known as adherens junctions and they typically include at least cadherin, β-catenin, and α-catenin. (wikipedia.org)
  • For instance, when an epithelial layer is complete and the adherens junctions indicate that the cell is surrounded, β-catenin may play a role in telling the cell to stop proliferating, as there is no room for more cells in the area. (wikipedia.org)
  • α-catenin participates in the formation and stabilization of adherens junctions by binding to β-catenin-cadherin complexes in the cell. (wikipedia.org)
  • Rituximab
  • Treatment of Children with Persistent and Chronic Idiopathic Thrombocytopenic Purpura: 4 Infusions of Rituximab and Three 4-Day Cycles of Dexamethasone. (nih.gov)
  • Rituximab destroys both normal and malignant B cells that have CD20 on their surfaces and is therefore used to treat diseases which are characterized by having too many B cells, overactive B cells, or dysfunctional B cells. (wikipedia.org)
  • malignant
  • She discovered that overexpression of ODC and c-Raf activation is sufficient to convert a normal primary keratinocyte into a malignant, invasive tumor cell. (wikipedia.org)
  • processes
  • The role of HAP1 in HD pathogenesis may involve aberration of cell cycle processes, as high immunostaining of HAP1 during the cell cycle has been observed. (wikipedia.org)
  • Tubulin and microtubule-mediated processes, like cell locomotion, were seen by early microscopists, like Leeuwenhoek (1677). (wikipedia.org)
  • Centrosome
  • Microtubules are nucleated and organized by microtubule organizing centers (MTOCs), such as the centrosome found in the center of many animal cells or the basal bodies found in cilia and flagella, or the spindle pole bodies found in most fungi. (wikipedia.org)
  • Recombinant
  • To solve this problem, recombinant E. coli overexpressing GadB was employed as a whole cell biocatalyst in buffer solution containing MSG. (springeropen.com)
  • cancers
  • It has been associated with an extensive number of cancers, neurodegenerative diseases, cell senescence, and aging. (wikipedia.org)
  • Found
  • In agreement with this hypothesis, we have found that palmitate inhibits adiponectin production by adipocytes in a cell-autonomous fashion. (pubmedcentralcanada.ca)
  • This indicates that Sulfolobus has a TCA cycle system similar to that found in mitochondria of eukaryotes. (wikipedia.org)
  • Most of these morphologies can be found as a life cycle stage in all trypanosomatid genera however certain morphologies are particularly common in a particular genus. (wikipedia.org)
  • survival
  • A proper balance between Bcl-2 and Bax determines cell survival and cell death. (hindawi.com)
  • 15-d-Δ12,14-PGJ2, Δ12-PGJ2, and PGJ2 share a common mono-unsaturated cyclopentenone structure as well as a set of similar biological activities including the ability to suppress inflammation responses and the growth as well as survival of cells, particularly those of cancerous or neurological origin. (wikipedia.org)
  • system
  • The central nervous system consists of two ganglia above the mouth, one on either side, connected to a nerve cord running back along its length to motor neurons and sensory cells in each segment. (wikipedia.org)
  • evidence
  • Gilmour provided the first evidence that elevated epidermal levels of polyamines alone can positively affect the recruitment of bulge stem cells in the skin. (wikipedia.org)
  • different
  • Interestingly, palmitate exerts its effect on fat cells at different levels. (pubmedcentralcanada.ca)
  • Different species go through a range of different morphologies at different stages of the life cycle, most have at least two different morphologies. (wikipedia.org)
  • indicates
  • mastig", meaning whip (referring to the flagellum), and a prefix which indicates the location of the flagellum on the cell. (wikipedia.org)
  • sufficient
  • The body therefore naturally produces anti-oxidants that neutralise "sweep up" these free radicals to prevent cells from becoming damaged but often not in sufficient quantities. (totalhealth.co.uk)
  • disease
  • Background Human being fetal midbrain-derived neural progenitor cells (NPCs) might deliver a cells source for medication testing and regenerative cell therapy to take care of Parkinsons disease. (biomasswars.com)
  • release
  • In kinetic partitioning, Hsp70s repetitively bind and release substrates in cycles that maintain low concentrations of free substrate. (wikipedia.org)
  • Species
  • This chapter presents biology and enzootic cycles of B. burgdorferi and related Borrelia species. (asmscience.org)
  • 2000 Genus Zelonia Shaw, Camargo et Teixeira 2016 Some trypanosomatids only occupy a single host, while many others are heteroxenous: they live in more than one host species over their life cycle. (wikipedia.org)
  • activity
  • It is possible that the non-pathogenic activity of HAP1 is intracellular trafficking and that this is perturbed following its association with mHtt. (wikipedia.org)