Amino Acids, BasicAmino Acid SequenceMolecular Sequence DataSerineAmino AcidsMutagenesis, Site-DirectedSequence Homology, Amino AcidBase SequenceAmino Acids, DiaminoBinding SitesCloning, MolecularMutationRecombinant ProteinsProtein Structure, TertiaryProtein BindingSubstrate SpecificityKineticsModels, MolecularPeptide FragmentsAmino Acid SubstitutionLysineCell LinePhosphorylationStructure-Activity RelationshipProtein ConformationPeptidesEscherichia coliAmino Acid Transport Systems, BasicArginineProtein PrecursorsProtein Processing, Post-TranslationalSequence AlignmentDNAPlasmidsAmino Acid MotifsCattleRecombinant Fusion ProteinsPhosphoserineSerine EndopeptidasesProtein Structure, SecondaryElectrophoresis, Polyacrylamide GelTyrosineMolecular WeightDNA, ComplementaryPeptide MappingThreonineTransfectionProtein KinasesPhosphoproteinsHeparinEnzyme ActivationConserved SequenceCOS CellsProtein-Serine-Threonine KinasesAlanineBacterial ProteinsCarrier ProteinsTrypsinCysteineSequence Homology, Nucleic AcidProteinsChromatography, High Pressure LiquidDNA PrimersHistidinePoint MutationMutagenesisDNA-Binding ProteinsCells, CulturedHydrogen-Ion ConcentrationMembrane ProteinsCell MembraneCatalysisBiological TransportHydrolysisHeLa CellsBlotting, WesternRestriction MappingRNA, MessengerSaccharomyces cerevisiaeCircular DichroismCricetinaeCyanogen BromidePhosphopeptidesAmino Acid Transport SystemsSequence DeletionSignal TransductionProtein Kinase CTranscription, GeneticEndopeptidasesCell NucleusCrystallography, X-RayRabbitsMacromolecular SubstancesCatalytic DomainMass SpectrometryNuclear Localization SignalsCytoplasmProlineSubtilisinsLeucine