Interspecific somatic cell. Medical search. Frequent questions

In the first sense of the term, a hybrid is the result of interbreeding between two animals or plants of different taxa , whether between individuals belonging to different species within the same genus (interspecific hybrids or crosses), different subspecies within the same species (intraspecific hybrids), or different genera (intergeneric hybrids). (newworldencyclopedia.org)
Interspecific plant hybrids sometimes can reproduce as a result of polyploidy , such as triticale, a polyploid hybrid of wheat and rye. (newworldencyclopedia.org)
These include crosses between organisms such as interspecific hybrids or crosses between difference races. (newworldencyclopedia.org)
Interspecific hybrids result from a cross between two species, normally from within the same genus . (newworldencyclopedia.org)
Mules, hinnies, and other normally sterile interspecific hybrids cannot produce viable gametes because the extra chromosome cannot make a homologous pair at meiosis , meiosis is disrupted, and viable sperm and eggs are not formed. (newworldencyclopedia.org)
Somatic cell hybrids and interspecific backcross analysis showed that Smage3 is autosomal and that Smage1 and Smage2 are located between the Dmd and the Ar loci on the mouse X chromosome. (unamur.be)
Effects of different levels of vitamins (thiamine, pyridoxine and nicotinic acid) and macro-elements (MS, NN and PG) on development of globular, heart- and torpedo-stage embryos and plantlets with green cotyledons and on shoot production from them were studied in grapevine interspecific hybrids: cvs. (kubsau.ru)
In vitro protocol optimization for development of interspecific hybrids of oil palm (Elaeis oleifera (H.B.K) Cortés x Elaeis guineensis Jacq. (arccjournals.com)
Pollen mother cells of 112-chromosome (2n + n) Saccharum officinarum X S. spontaneum hybrids usually showed 56II or 55II + 2I. (eurekamag.com)
Pollen mother cells of 136-chromosome (2n + n) S. officinarum X S. spontaneum hybrids showed a range of 66II + 4I to 61II + 14I. (eurekamag.com)
Upon being pollinated by F1 (2n + n) hybrids, S. officinarum transmitted 80 chromosomes, its somatic chromosome number, to its progenies. (eurekamag.com)

For SCNT, the chromosomes of the unfertilized canine oocytes were removed by micromanipulation, and a single donor cell was transferred into each enucleated oocyte. (nature.com)
Most of the unread genome just belonged to centromeric regions, these are special sections of chromosomes that are necessary for cell division, and they usually consist of tandem repeating DNA sequence units. (vechnayamolodost.ru)
Image analysis workflows to reveal the spatial organization of cell nuclei and chromosomes. (ipk-gatersleben.de)

Some oncogenic viruses that code for fusogens and are capable of hybridizing cells are: Epstein-Barr virus HPV Hepatitis B and Hepatitis C Human T-cell lymphotropic virus type 1 (HTLV-1) The virus used in laboratories for controlled cell hybridization is inactivated Sendai virus. (wikipedia.org)
Using polyethylene glycol is the method for inducing cell hybridization that requires the fewest steps. (wikipedia.org)
Interspecific hybridization of Alstroemeria for the development of new, ornamental plants. (cornell.edu)
During interspecific hybridization, often the embryo dies quite early so that no mature seeds can be obtained. (cbsetuts.com)
Somatic hybridization. (chanakyamandal.org)
Role of interspecific and intergeneric hybridization. (chanakyamandal.org)
This marker may be useful for monitoring interspecific hybridization and introgression in the southern pines. (researchgate.net)

Previously, intra- and interspecific canine embryos have been constructed by canine SCNT into canine and bovine oocytes, respectively, but this did not result in viable offspring 9 . (nature.com)
The use of somatic cell nuclear transfer to obtain interspecific cloned embryos from brown brocket deer karyoplast and bovine cytoplast: Embryo development and nuclear gene expression. (nupecce.com.br)
Steward (1963), Helperin, and Wetherell (1964) successfully developed somatic embryos from young anther cells of Datura. (cbsetuts.com)
Interspecific transplantation of polar plasm between Drosophila embryos. (uchicago.edu)
BC is a technique in which deletion of a key gene for the development of a specific lineage creates a vacant niche (organogenesis-disabled phenotype) that can be complemented by the progeny of wild type pluripotent stem cells injected into embryos at the blastocyst stage of development. (biomedcentral.com)

Practically, cycle regulation, chromosome stability and epigenetic F9 cells allow for the efficient metabolic labelling of the modification, in both mouse and human oocytes SILAC reference in vitro, overcoming the difficulty of directly labelling oocytes in vivo. (gotomydoctor.com)
Result] 1)The meiosis of pollen mother cells of C. cathayensis was divided into two stages, the chromosome number of daughter cells was reduced by half during the first division and not changed again during the second division. (linyekexue.net)
3)The chromosome behavior of most pollen mother cells in C. cathayensis was normal and paired as 16 bivalents at metaphaseⅠ. (linyekexue.net)
This study was conducted to obtain information about somatic chromosome numbers, karyotypes, and genome size of these five species. (ashs.org)

A molecular program that causes the interspecific barrier might be independent of genome-wide evolution. (frontiersin.org)
Genome variability in the somatic plant cells. (org.ua)
[1] Kunakh VA. Genome variability in plant somatic cells. (org.ua)
Most heritable information in eukaryotic cells is encoded in the nuclear genome, with inheritance patterns following classic Mendelian segregation. (nature.com)

Specific in vitro breeding techniques include somaclonal and gametoclonal variation, embryo culture, somatic embryogenesis, mutation breeding, meristem culture for the production of pathogen free plants, micropropagation, and in vitro fertilization. (cornell.edu)
Analysis of cell movements and fate mapping during early embryogenesis in Drosophila melanogaster. (uchicago.edu)

BC is the only current method for making fully functional, three-dimensional organs from pluripotent cells and generating human organs in large mammalian hosts may be able to address the critical worldwide problem of organ shortages for transplantation [ 16 ]. (biomedcentral.com)

Smage1/2 transcripts were detected in several tumor and embryonal cell lines but not in normal mouse tissues with the exception of testis. (unamur.be)
The technique of maintaining and growing cells, tissues, or organs, especially on artificial medium in suitable containers under controlled environmental conditions is known as tissue culture. (cbsetuts.com)
The explant may be of single isolated cells or tissues or any plant organ. (pdfcoffee.com)
The degree of totipotency is maximum in undifferentiated cell tissues. (pdfcoffee.com)
Sophisticated lab facilities are needed Plants cells, tissues and organs grown in artificial medium Optimum environmental conditions are provided Aseptic condition is maintained in tissue culture laboratory Plants are multiplied by asexual method/vegetative methods Progenies obtained are true to type because of asexual reproduction 5. (pdfcoffee.com)
Using GO analysis, these alterations do not relate in any simple way to the classic signature of ageing known from somatic tissues. (gotomydoctor.com)
Furthermore, we conclude that the classic features of ageing may not be transposed from somatic tissues to oocytes in a one-to-onefashion. (gotomydoctor.com)
Their team focuses on using human induced pluripotent stem cells to grow human tissues inside pigs. (the-scientist.com)
Human pluripotent stem cells harbor the potential to provide an inexhaustible supply of donor cells or tissues or organs for transplantation," Wu wrote in an email. (the-scientist.com)
This can range from the relatively pedestrian, such as a person who received a bone marrow transplant, to creatures that seem more at home in science fiction, such as animals containing cells or tissues belonging to other species. (the-scientist.com)
[4] Kunakh VA. Cytogenetic behaviour of tissue culture of Haplopappus Culture of isolated organs, tissues and cells of plants M.: Nauka, 1970:155-8. (org.ua)

Recent technologies, for example nuclear transfer and genetic induced reprogramming, have been employed to induce somatic cells into a pluripotent state similar to embryonic status. (fapesp.br)
Research interests are in the areas of new plant development and breeding, plant environment interactions, plant cell and tissue culture, in vitro plant breeding, plant propagation, genetic modifications for plant improvement, and plant growth and development of ornamental plants. (cornell.edu)
Data on the dynamics of the cell population genetic structure as well as the role and peculiarities of the selection effects in the course of the cell adaptation to in vitro conditions have been reviewed. (org.ua)
The physiological adaptation to the conditions of maintenance in vitro is observed on the early steps of the culturing, later on there occur the processes of the genetic adaptation manifested as a change in tlie cell population genetic structure. (org.ua)

The models use isolated primary support cells cultured onto a biological scaffold and within a microfluidic system to create a niche and support the desired differentiation of epithelia, germ and somatic cells from patient-derived induced pluripotent stem cells. (biomedcentral.com)
Hence, global gene expression and epigenetics of cells submitted to in vitro induction of pluripotency and subsequent differentiation into PGCCLs. (fapesp.br)
UNIT-1 Laboratory requirements for tissue culture-culture media and their constituents-cell culture types and applications of cell culture-cell and organ differentiation-somoclonal variation tissue culture technology in India-protoplast cultureregeneration. (pdfcoffee.com)
About karyotypic differentiation of callus cells in long-term culture. (org.ua)
Organogenesis is a complex developmental process requiring hierarchical cell and tissue differentiation, coordinated in time and space in response to changes in local and distant signaling cues. (biomedcentral.com)

Animals with human cells could provide donor organs or help us understand neuropsychiatric disorders. (the-scientist.com)
Other scientists, including Jun Wu, a stem cell biologist at the University of Texas Southwestern Medical Center, are also studying chimeras with the ultimate goal of one day being able to grow enough human organs to meet the enormous need for transplants, potentially saving hundreds of thousands of lives. (the-scientist.com)
since the new organs would be made from their own cells, they wouldn't have to worry about organ rejection. (the-scientist.com)
Some researchers are attempting to use stem cells to bioengineer human organs in the lab in vitro , rather than inside another species (5). (the-scientist.com)

[8] Gubar EK, Kunakh VA. Karyotype variability of cultured Crepis cells (Crepis capillaris L. Wallr. (org.ua)
Meiosis of Pollen Mother Cell and Karyotype of Carya cathayensis [J]. Scientia Silvae Sinicae, 2017, 53(11): 77-84. (linyekexue.net)

A particular organ (Reproductive or somatic) like root or shoot meristems or leaf primordia or floral buds is isolated and cultured. (cbsetuts.com)
Reproduction (2014) 148 55-72 (Thus, ageing of oocytes may The 'maternal age effect' in reproduction, characterized be viewed as a life-long maintenance of cellular by a negative relationship between maternal age and homeostasis in the same cell, unlike ageing of the male reproductive success, is a poorly understood phenom- germline. (gotomydoctor.com)

The first instance of intentionally generated hybrid cells was described in 1960 by Barski, Sorieul, and Cornefert in their paper "Production of cells of a 'hybrid' nature in cultures in vitro of 2 cellular strains in combination," originally published in French. (wikipedia.org)
[7] Kunakh VA. Polyploidy in cell culture in vitro and its possible causes. (org.ua)

Here we describe the cloning of two Afghan hounds by nuclear transfer from adult skin cells into oocytes that had matured in vivo . (nature.com)
Successful somatic-cell nuclear transfer (SCNT) depends on the quality, availability and maturation of the animal's unfertilized oocytes. (nature.com)
Figure 1: Dog cloned by somatic-cell nuclear transfer. (nature.com)
Finally, we discuss how both nuclear and cytoplasmic genomes have evolved to co-inhabit the same host cell via one of the longest symbiotic processes, and all the opportunities for intergenomic conflict that arise due to divergence in inheritance patterns. (nature.com)

Barley stripe mosaic virus-mediated somatic and heritable gene editing in barley (Hordeum vulgare L.). Front. (ipk-gatersleben.de)

As scientific understanding of stem cells, gene editing, and organism development improved, Garry felt that her career path was clear. (the-scientist.com)
Molecular genetics of the Drosophila melanogaster ovo locus, a gene required for sex determination of germline cells. (uchicago.edu)
Given the extreme toxicity of some of the toxins of these TA systems, we were curious how the cell silences toxins, if the antitoxin is inactivated or when toxins are obtained without antitoxins via horizontal gene transfer. (preprints.org)

The autonomous function of germ plasm in a somatic region of the Drosophila egg. (uchicago.edu)
Induction of germ cells at the anterior pole of the egg. (uchicago.edu)

On the other hand, a chimera is defined as an organism in which cells from two or more different organisms have contributed. (frontiersin.org)
Today, Garry and her husband Dan, a transplant cardiologist, are pioneers in the field of interspecies chimera research, the study of organisms containing cells from two different species. (the-scientist.com)

Polyethylene glycol functions by changing the direction and configuration of lipid molecules in the cell membrane, which increases their permeability and allows two membranes to fuse. (wikipedia.org)
Salt stress tolerance of potato plants were determined with visual damage scale, cell membrane injury analysis and malondialdehyde (MDA) content, the indicator of lipid peroxidation. (9lib.net)
The data indicated that the low cell membrane injury and MDA content made cvs. (9lib.net)

The vast majority of genes in eukaryotes are located within chromosomal structures in the nucleus of the cell. (nature.com)
Hearing depends on the mechano-sensory hair cells (HCs) and their innervating neurons, the spiral ganglion neurons (SGNs), which are responsible for transmitting auditory information from the HCs in the organ of Corti to the cochlear nucleus in the brainstem. (biomedcentral.com)
Using exogenous stem cells to replace lost inner ear neurons is a potential strategy if stem cell-derived neurons can form central and peripheral connections, form synapses on hair cells and cochlear nucleus neurons, and re-establish functional and tonotopic circuits [ 6 ]. (biomedcentral.com)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
We think that the developmental cues that exist in the pig will help to guide the human cells inside the porcine embryo. (the-scientist.com)
This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing. (biomedcentral.com)

Cell division cycle of cultured neural precursor cells from Drosophila. (uchicago.edu)

However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
INTRODUCTION TO TISSUE CULTURE Definition: Culture of plant cell, tissue and organ on artificial medium under aseptic condition is called tissue culture. (pdfcoffee.com)
Researchers began to dream of a future in which a patients' own cells, perhaps from the blood or the skin, could be converted into these induced pluripotent stem cells and grown into whatever organ the patient needed. (the-scientist.com)

Somatic cell parasitism and the evolution of somatic tissue compatibility. (ekt.gr)
It has been concluded that cell adaptation to the conditions of long-term maintenance in the passaged culture is the process of formation of the novel biological system as a result of the effect of the major driving factors of evolution, viz. (org.ua)

Donor fibroblasts were obtained from an ear-skin biopsy of a male Afghan hound and cultured for two to five passages (in which fully grown cells are transferred to a new culture dish). (nature.com)

These fusions can happen spontaneously as in the case of tumor hybrid cells, or may be induced by a variety of laboratory techniques. (wikipedia.org)
Tumor hybrid cells: fusion of tumor cells with normal bodily cells. (wikipedia.org)

To directly assess the functional effects of these mutations, site-directed mutant receptors were transiently expressed in CHO-K1 cells and cAMP accumulation stimulated by recombinant eelLH (rec-eelLH) was measured by homogeneous time-resolved fluorescence (HTRF) assays. (preprints.org)

Niche-associated activation of rac promotes the asymmetric division of Drosophila female germline stem cells. (uchicago.edu)
The receptor tyrosine phosphatase Lar regulates adhesion between Drosophila male germline stem cells and the niche. (uchicago.edu)

Coral recruitment: the importance of interspecific differences in juvenile growth and mortality. (ekt.gr)

Several studies have reported that induced pluripotente stem cells (iPSCs), similarly to embryonic stem cells (ES cells), are able to differentiate and give rise to primordial germinal stem cells (PGCs) and are even able to develop into gametes (oocytes and spermatozoa). (fapesp.br)

Cell division and cell cycle: Mitosis and meiosis, their regulation, steps in cell cycle, and control of cell cycle. (pathfinderacademy.in)
Method] 1)Male inflorescence of C. cathayensis was selected as material, DAPI fluorescent staining was used to study the meiosis of pollen mother cell of C. cathayensis . (linyekexue.net)
Conclusion] 1) Although there is the phenomenon of apomixis in C. cathayensis , it still follows the law of meiosis of pollen mother cells in most angiosperms. (linyekexue.net)
2) C. cathyensis pollen mother cell meiosis synchronization degree is very high, which is unfavorable to prolong its pollination period. (linyekexue.net)

In 2007, a team at Kyoto University created pluripotent stem cells from adult human somatic cells (4). (the-scientist.com)

Unlike the male germline, the bulk of The synthesis of the aforementioned molecular oocytes do not have a gonial stem cell population. (gotomydoctor.com)

Tissue culture is based on the concept of totipotency i.e. each cell has the ability to develop in to whole organism. (pdfcoffee.com)
[5] Sidorenko PG, Kunakh VA. Character of caryotype variability in cell population of tissue culture of Haplopappus gracilis with long term passaging. (org.ua)

We address these potential limitations by adopting the technique of blastocyst complementation (BC) to generate inner ear neurons from induced pluripotent stem cells (iPSCs). (biomedcentral.com)

In callus culture, the explant undergoes cell division and forms an unorganized and undifferentiated mass of cells called Callus. (cbsetuts.com)

Cancer Cell International. (wikipedia.org)

Cell structure, function and cell cycle. (helpstudentpoint.com)

Certain oncogenic viruses encode for fusogens, which are proteins that encourage two cell membranes to fuse. (wikipedia.org)
When the viral genes are expressed by the host cell, the membranes of two cells may fuse. (wikipedia.org)
After the cells are aligned, a pulse voltage around 10V can be applied, causing the membranes of these cells to fuse. (wikipedia.org)

The plant cell adaptation has been shown to be the multistage event. (org.ua)

By 1952, the evidence of various forms of cytoplasmically inherited elements (CIEs) had grown, leading Joshua Lederberg to synthesise the inheritance of cellular organelles and symbionts into one framework in his treatise "Cell genetics and hereditary symbiosis" (Lederberg 1952 ). (nature.com)

3) The abnormal mother cell of C. cathyensis mainly appears in the two stages of metaphase Ⅰ and metaphase Ⅱ. (linyekexue.net)

Cell signaling: Hormones and their receptors, cell surface receptor, signaling through G-protein coupled receptors, signal transduction pathways, second messengers, regulation of signaling pathways, bacterial and plant two-component signaling systems, bacterial chemotaxis and quorum sensing. (pathfinderacademy.in)

By integrating these chromatin "signals to promoters" data and transcriptomics data (analysis of RNA transcripts of brain cells from many individuals), we have shown the possibility of discovering new genes that have not yet been annotated. (vechnayamolodost.ru)
Analysis of somatic metaphases resulted in formulas of 20m + 2sm for L. camara 'Lola' and 'Denholm White', 12m + 12sm for L. canescens , 44m for L. depressa var. (ashs.org)

Modern Cell Theory contains 4 statements, in addition to the original Cell Theory: The cell contains hereditary information (DNA) which is passed on from cell to cell during cell division. (pdfcoffee.com)

Its activity is also high in animal and human melanoma [19, 20], the cells of which are probably all derived from the neural crest cell-system. (science-connections.com)

Transplantation of Fetal Hematopoietic Stem Cells in Utero: The Creation of Hematopoietic Chimeras. (ekt.gr)

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. (biomedcentral.com)

However, advances in stem cell research in the 2000s revolutionized the field, opening up new possibilities and new applications for multispecies organism research. (the-scientist.com)

Innate and adaptive immune system: Cells and molecules involved in innate and adaptive immunity, antigens, antigenicity and immunogenicity. (pathfinderacademy.in)

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