Antigen Kiel 67, also known as Ki-67 or MKI67 (marker of proliferation Kiel 67), is a protein that in humans is encoded by the MKI67 gene (antigen identified by monoclonal antibody Ki-67). (wikipedia.org)
The Ki-67 protein was originally defined by the prototype monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the Hodgkin lymphoma cell line L428. (wikipedia.org)
The following product was used in this experiment: Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 615, eBioscience™ from Thermo Fisher Scientific, catalog # 366-5698-82, RRID AB_2925428. (thermofisher.com)
Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody, Brilliant Ultra Violet 615. (thermofisher.com)
Description: The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein. (thermofisher.com)
The FKELF theme, which binds the initial Ki-67 monoclonal antibody, can be indicated by an asterisk. (angiogenesis-blog.com)
The receiver operating characteristic (ROC) curve was used to estimate the accuracy of each ADC parameter in predicting the Ki-67 level, and finally a multivariate logistic regression model was constructed based on the results of ROC analysis. (biomedcentral.com)
The AUROC of Ki-67 LI level assessed by the multivariate logistic regression model was 0.800 (95%CI 0.721-0.879). (biomedcentral.com)
There was a negative correlation between ADC parameters and Ki-67 LI, and the multivariate logistic regression model combined with peritumoral edema and ADC parameters could improve the prediction ability of Ki-67 LI. (biomedcentral.com)
Tumor cell proliferation by Ki-67 had significant prognostic impact by multivariate analysis. (biomedcentral.com)
Proliferation9
Antigen KI-67 is a nuclear protein that is associated with cellular proliferation and ribosomal RNA transcription. (wikipedia.org)
Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis, but does not significantly affect cell proliferation in vivo: Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. (wikipedia.org)
Ki-67 and MIB-1 monoclonal antibodies are directed against different epitopes of the same proliferation-related antigen. (wikipedia.org)
Ki-67 labeling index (LI) is an important indicator of tumor cell proliferation in glioma, which can only be obtained by postoperative biopsy at present. (biomedcentral.com)
Ki-67 is a non-nuclear protein expressed in proliferating cells, which is related to cell proliferation, differentiation, metastasis, and apoptosis. (biomedcentral.com)
The aim of the present study was to evaluate the prognostic impact of mitotic count, Ki-67 expression and novel proliferation markers phosphohistone H3 (PHH3), minichromosome maintenance protein 4 (MCM4) and mitosin, and to compare the results with histopathological variables. (biomedcentral.com)
The proliferation marker Ki-67 is expressed in all phases of the cell cycle [ 12 ], and elevated Ki-67 in tumor cells was associated with the most aggressive melanomas in our previous study [ 13 ]. (biomedcentral.com)
The same research reported that overexpression of the fragment from the Ki-67 N-terminus improved mobile proliferation [33]. (angiogenesis-blog.com)
Tumor6
The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
There is tumor cell positivity in 70% of the cells: Ki-67 labelling index = 70% Counting positive versus negative nuclei with Ki-67 labeling, in this case in a neuroendocrine tumor of the small intestine. (wikipedia.org)
and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. (thermofisher.com)
Increased mitotic count and elevated Ki-67 expression were strongly associated with increased tumor thickness, presence of ulceration and tumor necrosis. (biomedcentral.com)
High expression of PHH3 and MCM4 was correlated with high mitotic count, elevated Ki-67 expression and tumor ulceration, and increased PHH3 frequencies were associated with tumor thickness and presence of tumor necrosis. (biomedcentral.com)
Mitosis4
Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
Although Ki-67 positivity is a marker of proliferative cells, it is uncertain how many of the cells expressing Ki-67 will actually undergo mitosis. (biomedcentral.com)
The Ki-67 do it again area consists of residues phosphorylated by CDK1 during mitosis [3 also,52,53]. (angiogenesis-blog.com)
Shown to interact2
Ki-67 (protein) has been shown to interact with CBX3. (wikipedia.org)
Ki-67 has been shown to interact with the DNA-bound protein chromobox protein homolog 3 (CBX3) (heterochromatin). (thermofisher.com)
Nucleus1
Does NuMa have a scaffold function in the interphase nucleus. (mpg.de)
Mitotic3
Furthermore, high mitotic count and elevated Ki-67 expression were also associated with Clark's level of invasion and presence of vascular invasion. (biomedcentral.com)
Univariate analyses showed a worse outcome in cases with elevated Ki-67 expression and high mitotic count, whereas PHH3, MCM4 and mitosin were not significant. (biomedcentral.com)
Ki-67 was a stronger and more robust prognostic indicator than mitotic count in this series of nodular melanoma. (biomedcentral.com)
Growth fraction of a given2
Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
Ki-67 is used as a marker for determining the growth fraction of a given population of cells. (thermofisher.com)
Predict1
This study aimed to explore the correlation between Ki-67 LI and apparent diffusion coefficient (ADC) parameters and to predict the level of Ki-67 LI noninvasively before surgery by multiple MRI characteristics. (biomedcentral.com)
Dividing cells show strong Ki-67 staining in cell nuclei while all cells contain large amounts of tubulin, the major component of microtubules. (wikipedia.org)
Evidence of proliferating endothelial cells was determined by the presence of Ki-67 antigen-positive cells, bromodeoxyuridine-positive cells, or [ 3 H] thymidine-positive cells. (biomedcentral.com)
Human3
The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. (thermofisher.com)
This do it again region exists in all noticed isoforms of human being Ki-67 and, since it can be contained within an individual exon, it really is there completely [33] always. (angiogenesis-blog.com)
Cell2
Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. (wikipedia.org)
In a small study of melanocytic lesions, PHH3 was a useful supplement in differentiating malignant melanoma from benign nevi, although it did not show any advantage compared to Ki-67 [ 20 ]. (biomedcentral.com)
Positive1
In breast cancer Ki67 identifies a high proliferative subset of patients with ER-positive breast cancer who derive greater benefit from adjuvant chemotherapy. (wikipedia.org)
Index1
MIB-1 is used in clinical applications to determine the Ki-67 labelling index. (wikipedia.org)
High3
High Ki-67 expression in an invasive breast cancer, with cancer nuclei being stained (brown). (wikipedia.org)
The differences in MRI characteristics were compared between the low and high Ki-67 LI groups. (biomedcentral.com)
The Ki-67 LI of low-grade gliomas (LGGs) was different from that of high-grade gliomas (HGGs), and the cut-off point of Ki-67 LI for distinguishing LGGs from HGGs was 9.5%, with an area under the ROC curve (AUROC) of 0.962 (95%CI 0.933-0.990). (biomedcentral.com)
Green2
Immunofluorescent antibody staining against neurofilament (green) and Ki-67 (red) in a mouse embryo 12.5 days after fertilization. (wikipedia.org)
The PP1-binding site (green) can be accompanied from the crystal framework from the Ki-67 (green):PP1(gray) holoenzyme complicated (PDB:5J28) [35]. (angiogenesis-blog.com)
Expression1
The advanced pathological grade was associated with strong TGFB1, VEGFA, and Ki67 protein expression and absent Tp53 staining. (hindawi.com)
Sections3
Ki-67 and MIB-1 may be used on fixed sections. (wikipedia.org)
One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
Automated grading of astrocytomas based on histomorphometric analysis ofKi-67 and Feulgen stained paraffin sections. (ntnu.edu.tw)
4. Immunohistochemical analysis of proliferating cell nuclear antigen, p53 protein and nm23 protein, and nuclear DNA content in transitional cell carcinoma of the bladder. (nih.gov)
8. Correlation of morphometry, nucleolar organizer regions, proliferating cell nuclear antigen and Ki67 antigen expression with grading and staging in urinary bladder carcinomas. (nih.gov)
9. Comparative assessment of proliferating cell nuclear antigen immunostaining and of nucleolar organizer region staining in transitional cell carcinomas of the urinary bladder. (nih.gov)
12. Relationship of genetic instability with immunoreactivities for p53 protein and proliferating cell nuclear antigen in transitional cell carcinoma of the bladder. (nih.gov)
A study in 41 specimens of DNA status, proliferating cell-nuclear antigen immunostaining, and argyrophilic nucleolar-organizer region counts. (nih.gov)
Antibody3
One of its primary advantages over the original Ki-67 antibody (and the reason why it has essentially supplanted the original antibody for clinical use) is that it can be used on formalin-fixed paraffin-embedded sections, after heat-mediated antigen retrieval (see next section below). (wikipedia.org)
Immunofluorescent antibody staining against neurofilament (green) and Ki-67 (red) in a mouse embryo 12.5 days after fertilization. (wikipedia.org)
The SolA15 antibody also recognizes human, non-human primate and canine Ki-67. (thermofisher.com)
Tumor cells2
The fraction of Ki-67-positive tumor cells (the Ki-67 labeling index) is often correlated with the clinical course of cancer. (wikipedia.org)
In studies of tumor cells, the "Ki-67 labeling index" refers to the number of Ki-67 positive cells within the population and this is used to predict outcome of particular cancer types. (thermofisher.com)
Nuclear protein1
Antigen KI-67 is a nuclear protein that is associated with cellular proliferation and ribosomal RNA transcription. (wikipedia.org)
Protein5
The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation, and can be used in immunohistochemistry. (wikipedia.org)
Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting (quiescent) cells (G0). (wikipedia.org)
Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. (wikipedia.org)
In attempts to identify cell-surface receptors for Wnt proteins, we have developed several bioassays for Wnt genes and learned to make cell-free Wnt protein in a complex with the surface antigen of hepatitis B virus. (nih.gov)
Synthesis2
Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis, but does not significantly affect cell proliferation in vivo: Ki-67 mutant mice developed normally and cells lacking Ki-67 proliferated efficiently. (wikipedia.org)
We analyzed the presence of the t(14;18)(q32;q21) as a surrogate marker of the malignant clone in cells of the FL microenvironment using combined fluorescence immunophenotyping and interphasecytogenetics targeting the BCL2 gene locus. (biomedcentral.com)
Progression1
Progression through checkpoints during interphase is regulated by cyclin-dependent kinases (CDKs), which are activated when bound by specific cyclins (see table below). (biolegend.com)
Marker2
Ki-67 is an excellent marker to determine the growth fraction of a given cell population. (wikipedia.org)
Ki-67 is used as a marker for determining the growth fraction of a given population of cells. (thermofisher.com)
Phases1
Interphase - During interphase, which is comprised of G1, S, and G2 phases, the cell prepares to undergo division by replicating its genome and organelles. (biolegend.com)
Count1
15. Correlation of mitotic indices, AgNor count, Ki-67 and Bcl-2 with grade and stage in papillary urothelial bladder cancer. (nih.gov)
Growth1
Cells can divide in response to stimuli such as growth factors and cytokines, or specific antigens. (biolegend.com)