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  • desmosomal
  • Immunofluorescence analysis, using the antifusion protein antibody, demonstrated pinin at lateral epithelial boundaries, which is consistent with desmosomal localization. (rupress.org)
  • cells
  • Immunoelectron microscopy of whole mount cytoskeletons from various cultured cell lines (rat glioma C6, mouse BALB/c 3T3, and Chinese hamster ovary) and quick-frozen, deep-etched replicas of Triton X-100-extracted rat embryo fibroblast cells revealed that plectin was primarily located at junction sites and branching points of intermediate filaments. (rupress.org)
  • The coexpression of C and D in all epidermal cells and the obligatory heteropolymeric IF assembly of the recombinant proteins argue for keratin orthologs, but the sequences do not directly reveal which protein behaves as a keratin I or II ortholog. (semanticscholar.org)
  • These antibodies reacted with the 46,000 and a 40,000 mol wt protein from Kc cells. (semanticscholar.org)
  • Heat-shock and cadmium chloride increase the vimentin mRNA and protein levels in U-937 human promonocytic cells. (semanticscholar.org)
  • The spatial distribution of polyribosomes in 3T3 cells and the associated assembly of proteins into the skeletal framework. (semanticscholar.org)
  • Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells. (semanticscholar.org)
  • A stable clone established from PC12 cells overexpressing a GFP-Peripherin fusion protein (pEGFP-Peripherin) was constructed for determining the pathway involved in neurodegeneration by biochemical, cell biology, and electronic microscopy approaches. (biomedcentral.com)
  • Given this, it is of interest to define the contribution of IF proteins in the response of cells and tissues to injury. (rupress.org)
  • Intermediate filaments (IFs) are cytoskeletal components found in the cells of vertebrate animal species, and perhaps also in other animals, fungi, plants, and unicellular organisms. (wikipedia.org)
  • Vimentin, the most widely distributed of all IF proteins, can be found in fibroblasts, leukocytes, and blood vessel endothelial cells. (wikipedia.org)
  • Synemin is an intermediate filament (IF) and, like other IFs, primarily functions to integrate mechanical stress and maintain structural integrity in eukaryotic cells. (wikipedia.org)
  • These intermediate filament proteins are expressed mostly in nerve cells where they are implicated in the radial growth of the axon. (wikipedia.org)
  • Nestin is an intermediate filament protein expressed in dividing cells during the early stages of development in the central nervous system (CNS), peripheral nervous system (PNS) and in myogenic and other tissues. (wikipedia.org)
  • Nestin, a protein marker for neural stem cells, is also expressed in follicle stem cells and their immediate, differentiated progeny. (wikipedia.org)
  • Green fluorescent protein (GFP), whose expression is driven by the nestin regulatory element in transgenic mice, serves to mark hair follicle stem cells. (wikipedia.org)
  • Stellate cells express smoothened (Smo) and patched-1 (Ptch1) proteins, which are significant features of the hedgehog receptor system. (wikipedia.org)
  • In order for a LCLC to be subclassified as the rhabdoid phenotype variant, at least 10% of the malignant tumor cells must contain distinctive structures composed of tangled intermediate filaments that displace the cell nucleus outward toward the cell membrane. (wikipedia.org)
  • Microscopic characteristics of rhabdoid cells include: Oval to polygonal cell shape Eosinophilic, hyaline-like perinuclear agglomerations of intermediate filaments Compressed, eccentric nuclei Prominent central macronucleoli (one or two) Abundant eosinophilic cytoplasm Reticular chromatin pattern The differential diagnosis of LCLC-RP includes secondary metastatic lesions, melignant melanoma of the lung with rhabdoid phenotype, mucinous adenocarcinomas (particularly those featuring signet-ring cells), rhabdomyosarcoma, epitheloid angiosarcoma, pleural mesothelioma, and plasmacytoma. (wikipedia.org)
  • At the hemidesmosome, plectin and BPAG1 associate with transmembrane proteins α6β4 integrin, a type of cell adhesion molecule, and BP180/collagen XVII, linking K5/K14 filaments in the basal cells to the basal lamina. (wikipedia.org)
  • Myomesin-1 is expressed in muscle cells and functions to stabilize the three-dimensional conformation of the thick filament. (wikipedia.org)
  • Additional studies have shown that gamma-actin colocalizes with alpha-actinin and GFP-labeled gamma actin localized to Z-discs, whereas GFP-alpha-actin localized to pointed ends of thin filaments, indicating that gamma actin specifically localizes to Z-discs in striated muscle cells. (wikipedia.org)
  • PMID
  • IF proteins have a very strong tendency to dimerize via the formation of an alpha-helical coiled coil (CC) by their rod domains [ PMID: 22869704 ]. (ebi.ac.uk)
  • sequences
  • The N and C-termini of IF proteins are non-alpha-helical regions and show wide variation in their lengths and sequences across IF families. (wikipedia.org)
  • interactions
  • IF central rods contain heptad repeats (repeating seven residue patterns) of hydrophobic resides that allow two different IF proteins to intertwine into a coiled-coil formation via hydrophobic interactions. (wikipedia.org)
  • The coiled-coil dimers undergo stepwise assembly and combine in an antiparallel manner, forming end-to-end interactions with other coiled-coils to form large 10 nm intermediate filaments. (wikipedia.org)