Epithelial cellsCytokinesProteinCytokineSecretionInducesTumor necroGene polymorphismsNeutrophilsCXCL8PromoterReceptor BetaSerumChemotacticExpressionELISAAnalogues was observedStimulatesPathwayProliferationProinflammatoryReceptorsHumansConcentrationsConcentrationMacrophageMacrophagesInflammationRole in the pathogenesisCXCR1ChemotaxisPeptideHumanCXCR2ModulationColiBiochemicalBaselineRegulationSignificantlyCellsPotentAssayProduction
Epithelial cells8
- Interleukin 8 (IL-8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. (wikipedia.org)
- IL-8 was found in neutrophils, epithelial cells, and macrophages. (nih.gov)
- Modulation of Helicobacter pylori induced interleukin-8 synthesis in gastric epithelial cells mediated by cag PAI encoded VirD4 homologue. (bmj.com)
- Our results support the concept of a possible link between microRNA expression and IL-8 induction in bronchial epithelial cells infected with P. aeruginosa. (univr.it)
- Incubation of tracheal aspirates with 16HBE14o- human bronchial epithelial cells increased IL-8 protein in both cell lysates and supernatants, as well as transcription from the IL-8 promoter. (utmb.edu)
- Phospholipase A(2) activation by poultry particulate matter is mediated through extracellular signal-regulated kinase in lung epithelial cells: regulation of interleukin-8 release. (cdc.gov)
- Hence, it is hypothesized in this article that poultry PM induces the release of interleukin-8 (IL-8) by lung epithelial cells that is regulated upstream by the concerted action of cytosolic phospholipase A2 (cPLA2) and extracellular signal-regulated kinase (ERK). (cdc.gov)
- For the first time, this study demonstrated that the poultry PM-induced IL-8 secretion by human lung epithelial cells was regulated by cPLA2 activation through ERK-mediated serine phosphorylation, suggesting a mechanism of airway inflammation among poultry farm workers. (cdc.gov)
Cytokines11
- As both interleukin-1-alpha and interleukin-8 provoke proliferation of keratinocytes, it is not unreasonable to propose that these two cytokines play a profound role in the enhancement of keratinocyte proliferation as a result of He-Ne laser irraditiation. (eurekamag.com)
- Concentration of two cytokines, interleukin-6 and interleukin-8, have been found in two different studies to be potential biomarkers for incidence of dry AMD. (preventblindness.org)
- Amniotic fluid interleukin 6 and interleukin 8 are superior predictors of fetal lung injury compared with maternal or fetal plasma cytokines or placental histopathology in a nonhuman primate model. (bvsalud.org)
- Amniotic fluid interleukin 8 , a proinflammatory cytokine , was previously correlated with the development of neonatal bronchopulmonary dysplasia , but whether amniotic fluid cytokines or placental pathology more accurately predicts neonatal lung pathology and morbidity is unknown. (bvsalud.org)
- Amniotic fluid and maternal and fetal plasma samples were collected after inoculation, and proinflammatory cytokines ( tumor necrosis factor alpha , interleukin beta, interleukin 6 , interleukin 8 ) were measured by a multiplex assay. (bvsalud.org)
- Amniotic fluid interleukin 6 and interleukin 8 levels were superior predictors of fetal lung injury than placental histopathology or maternal plasma cytokines . (bvsalud.org)
- We sought to determine the cytokines responsible for IL-8 expression in this context. (utmb.edu)
- Has been involved, along with HHV-8 viral proteins pro-inflammatory and / or other angiogenic, in HHV-8-related Kaposi's sarcoma, primary effusion lymphoma (PEL), and multicentric Castleman's disease (MCD), in addition to an MCD -related disorders involving elevation of systemic pro-inflammatory cytokines, including human vIL-6 and IL-6 (HIL-6). (ilgenetics.org)
- Additionally, some cytokines found to play an important role in human inflammation such as IL-8 7 , are not expressed by mice. (nature.com)
- The fibroblasts then produce hyaluronan and the cytokines interleukin (IL)-1 beta, IL-6, IL-8, IL-16, tumor necrosis factor (TNF) alpha, and CD4 ligand. (medscape.com)
- Previously, we have shown in a mouse model that exposure to hog dust extract (HDE) collected from a CAFO results in the activation of protein kinase C (PKC), elevated lavage fluid cytokines/chemokines including interleukin-6 (IL-6), and the development of significant lung pathology. (cdc.gov)
Protein11
- In humans, the interleukin-8 protein is encoded by the CXCL8 gene. (wikipedia.org)
- Das reife IL - 8 entfaltet seine Wirkung nach der Bindung an zwei G-Protein-gekoppelte Rezeptoren, welche auf der Zelloberfläche lokalisiert sind. (hoelzel-biotech.com)
- Interleukin-8 is a chemokine, a type of protein that aids the body's immune response to infections. (walkinlab.com)
- IL-8 protein abundance was high in tracheal aspirates from ventilated premature infants (mean, 5806 ± 4923 pg/mL). (utmb.edu)
- contributions interaction of vIL-6 protein to HHV-8 lytic biology. (ilgenetics.org)
- The receptor usage of the CXC chemokine granulocyte chemotactic protein-2 (GCP-2) was compared with that of interleukin-8 (IL-8) and epithelial-cell-derived neutrophil attractant-78 (ENA-78). (tau.ac.il)
- Here, we report that protein kinase Cδ (PKCδ) mediates LPA-induced NF-κB transcription and interleukin-8 (IL-8) secretion in HBEpCs. (johnshopkins.edu)
- Treatment of HBEpCs with LPA increased both IL-8 gene and protein expression, which was coupled to G i and G 12/13 proteins. (johnshopkins.edu)
- Intratracheal administration of LPA in mice resulted in elevated levels of macrophage inflammatory protein-2, a murine homolog of IL-8, and an influx of neutrophils in the bronchoalveolar lavage fluid. (johnshopkins.edu)
- Our results provide the first evidence for a direct interaction between a parasite-derived chemotactic factor and the host's chemotactic network, via a novel G protein-coupled receptor which interacts with the IL-8 receptor pathway. (ed.ac.uk)
- In human monocyte derived macrophages hypoxia rapidly upregulated IL-8 protein (within 2 hours) and mRNA expression (within 30 mins). (ox.ac.uk)
Cytokine6
- Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. (hoelzel-biotech.com)
- A report in the February 28, 2008 online British Journal of Ophthalmology revealed that at least some cases of age-related macular degeneration may stem from genetically driven production of another cytokine, interleukin-8. (preventblindness.org)
- The -251AA genotype of the interleukin-8 promoter gene has previously been found to promote release of interleukin-8, an inflammatory cytokine. (preventblindness.org)
- Human herpesvirus 8 (HHV-8) virus interleukin-6 (vIL-6) is a cytokine that is secreted and most poorly translated into the endoplasmic reticulum (ER). (ilgenetics.org)
- Both the pattern of cytokine expression and transcription factor activation by hypoxia was different to that seen with endotoxin.ConclusionsRapidly raised intrapulmonary IL-8 levels are associated with ARDS progression in patients with major trauma. (ox.ac.uk)
- Additionally, alcohol inhibits the lipopolysaccharide (LPS)-induced inflammatory cytokine, interleukin-6 (IL-6), via the p38 ERK1/2 MAPK pathway [ 9 ]. (cdc.gov)
Secretion5
- The effects of interleukin 1 (IL-1) on glucose-induced insulin secretion from isolated rat islets of Langerhans have been examined. (diabetesjournals.org)
- We've demonstrated that LPA (lysophosphatidic acidity)-induced IL (interleukin)-8 secretion was partly mediated via transactivation of EGFR [EGF (epidermal development aspect) receptor] in HBEpCs (individual bronchial epithelial primary cells). (brain-tumor-cancer-information.com)
- C5a, a potent peptide chemoattractant, stimulates interleukin-8 (IL-8) secretion from peripheral blood mononuclear cells (PBMC). (elsevierpure.com)
- IL-8 secretion was accompanied by accumulation of IL-8 mRNA in the cytosol and by nuclear expression of a κB DNA binding activity within 30 minutes. (elsevierpure.com)
- TNF-α-activated endothelial cells promote VSMC proliferation and secretion of ICAM-1 and IL-8 by elevating IL-6 release. (geneticsmr.com)
Induces3
- In target cells, IL-8 induces a series of physiological responses required for migration and phagocytosis, such as increases in intracellular Ca2+, exocytosis (e.g. histamine release), and the respiratory burst. (wikipedia.org)
- Because interleukin-1-alpha induces keratinocyte migration, this finding may partially explain the stimulatory effects on the motility of keratinocytes. (eurekamag.com)
- Outcomes LPA induces COX-2 appearance and PGE2 discharge We have showed previous that LPA treatment induces IL-8 [15-18] and IL-13R= 3). (brain-tumor-cancer-information.com)
Tumor necro1
- Differences in interleukin-8 and tumor necrosis factor-alpha in induced sputum from patients with chronic obstructive pulmonary disease or asthma. (hoelzel-biotech.com)
Gene polymorphisms2
- Interleukin-8 (IL-8) gene polymorphisms have been considered as susceptibility factors in periodontal disease. (nih.gov)
- Interferon gamma and interleukin 8 gene polymorphisms in patients with" by Enji Ahmed Mahmoud, Noha Adel Azab et al. (bue.edu.eg)
Neutrophils4
- The epithelial IL-8 expression did not correlate with the numbers of neutrophils, macrophages, CD45RO+, CD8+, or mast cells. (nih.gov)
- ATC/TTC haplotype cells significantly increased transmigration of neutrophils confirming the functional role for this IL-8 haplotype. (nih.gov)
- Exposing neutrophils in vitro to a proinflammatory micromilieu with N-formyl-Met-Leu-Phe, LPS, or IL-8 resulted in a diminished response regarding the increase in cellular size and pH. (uni-ulm.de)
- Last, the LPS-mediated impairment of the IL-8-induced response in neutrophils was confirmed in a translational, animal-free human whole blood model. (uni-ulm.de)
CXCL81
- Interleukin - 8 (IL - 8), auch bekannt unter dem Namen CXCL8, gehört zu der Familie der proinflammatorischen CXC Chemokine. (hoelzel-biotech.com)
Promoter6
- IL-8 is also known to be a potent promoter of angiogenesis. (wikipedia.org)
- NF-κB signal is essential for flagellin-dependent activation of the IL-8 promoter by L. pneumophila . (biomedcentral.com)
- E) Overexpression of dominant negative mutants inhibits L. pneumophila -induced activation of the IL-8 promoter. (biomedcentral.com)
- Mutation of the IL-8 promoter NF-κB site abolished aspirate-induced IL-8 transcription. (utmb.edu)
- Endotoxin concentrations in the tracheal aspirates were negligible and incapable of inducing IL-8 promoter activity. (utmb.edu)
- The correlation between C5a- induced κB binding activity and IL-8 gene expression was examined in the RAW264.7 macrophage cells using reporter genes directed by the κB sequence from IκBα and IL-8 promoter regions. (elsevierpure.com)
Receptor Beta7
- Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Interleukin 8 Receptor Beta (IL8Rb) in serum, plasma, tissue homogenates, cell lysates and other biological fluids. (scalegen.com)
- Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Interleukin 8 Receptor Beta (IL8Rb) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (scalegen.com)
- Description: A sandwich ELISA kit for detection of Interleukin 8 Receptor Beta from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (scalegen.com)
- Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 Receptor Beta (IL8Rb) were tested 20 times on one plate, respectively. (biomatik.com)
- Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 Receptor Beta (IL8Rb) were tested on 3 different plates, 8 replicates in each plate. (biomatik.com)
- This assay has high sensitivity and excellent specificity for detection of Interleukin 8 Receptor Beta (IL8Rb). (biomatik.com)
- No significant cross-reactivity or interference between Interleukin 8 Receptor Beta (IL8Rb) and analogues was observed. (biomatik.com)
Serum4
- This ELISA kit applies to the in vitro quantitative determination of Rabbit IL-8 concentrations in Serum, plasma and other biological fluids. (caslab.com)
- Description: A sandwich ELISA kit for detection of Interleukin 18 from Guinea pig in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (ibiomagazine.org)
- Serum interleukin-8 (IL-8) levels and tumor neutrophil infiltration are associated with worse prognosis in advanced cancers. (cun.es)
- Here, using a large-scale retrospective analysis, we show that elevated baseline serum IL-8 levels are associated with poor outcome in patients (n = 1,344) with advanced cancers treated with nivolumab and/or ipilimumab, everolimus or docetaxel in phase 3 clinical trials, revealing the importance of assessing serum IL-8 levels in identifying unfavorable tumor immunobiology and as an independent biomarker in patients receiving immune-checkpoint inhibitors. (cun.es)
Chemotactic2
- IL-8, also known as neutrophil chemotactic factor, has two primary functions. (wikipedia.org)
- cellular shape, and chemotactic activity elicited by IL-8. (uni-ulm.de)
Expression15
- DMSO was found to directly inhibit IL-8 expression at the level of transcription. (jci.org)
- The results revealed (i) a significant increase in interleukin-1-alpha and interleukin-8 production and their respective mRNA expression in He-Ne laser treated groups as compared with nonirradiated controls, and (ii) under 1.5 joules/cm-2 irradiation, this stimulating effect of He-Ne laser treatment is concentration-dependent. (eurekamag.com)
- Nach der Expression wird IL - 8 jedoch auf entweder 77 Aminosäuren (in Nichtimmunzellen) oder 72 Aminosäuren (in Monozyten und Makrophagen) gekürzt. (hoelzel-biotech.com)
- Die Expression von IL - 8 wird durch vielzählige Faktoren beeinflusst. (hoelzel-biotech.com)
- In this study we analyzed the microRNA profile of cystic fibrosis (CF) bronchial epithelial IB3-1 cells infected with Pseudomonas aeruginosa by microarray and quantitative RT-PCR, demonstrating that microRNA 93 (miR-93), which is highly expressed in basal conditions, decreases during infection in parallel with increased expression of the IL-8 gene. (univr.it)
- Tracheal aspirate IL-8 concentration and airway epithelial cell IL-8 expression are each increased in premature infants undergoing mechanical ventilation. (utmb.edu)
- We conclude that airway fluid from mechanically ventilated premature infants contains soluble factors capable of inducing airway epithelial cell IL-8 expression via a NF-κB-dependent pathway, and that IL-1β plays a specific role in this process. (utmb.edu)
- however, its delayed expression (maximal at 4 hours) suggested a less important role in the rapid production of IL-8. (elsevierpure.com)
- Pertussis toxin, which ADP-ribosylates the G(i) proteins known to couple to the C5a receptor, produced minimal inhibition of C5a- induced IL-8 expression and had little effect on C5a-induced calcium mobilization in RAW264.7 cells. (elsevierpure.com)
- These results suggest that NF-κB activation is required for C5a-induced IL-8 gene expression and that this response is mediated primarily through a pertussis toxin-insensitive pathway. (elsevierpure.com)
- Endometrial biopsies were collected from 6 RM and 9 SM during oestrus and 24 h after infusion of frozen/thawed spermatozoa and the mRNA expression of TLR4 and IL-8 was quantified by rt-PCR. (aber.ac.uk)
- Expression of the pro-inflammatory chemokineIL-8 was measured to confirm the status of an inflamed uterus. (aber.ac.uk)
- IL-8 and other proinflammatory mediator expression was measured by ELISA, northern blotting or multi-probe RNase protection assay.ResultsIn patients with major trauma, IL-8 levels were significantly higher in patients that progressed to ARDS compared to those that did not (n = 56, P = 0.0001). (ox.ac.uk)
- Acute hypoxia also increased rabbit alveolar macrophage IL-8 expression. (ox.ac.uk)
- Hypoxia induced HIF-1 expression, but cobaltous ions and desferrioxamine did not mimic hypoxic IL-8 induction. (ox.ac.uk)
ELISA4
- The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit IL-8. (caslab.com)
- In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
- Dans la présente étude, les taux de production des interleukines 12 et 13 (IL-12 et IL-13) ont été mesurés à l'aide de la méthode ELISA dans les surnageants de culture de cellules mononucléées de sang périphérique stimulées par des mitogènes chez 30 cas de bêta-thalassémie non splénectomisés présentant une surcharge martiale ainsi que chez 20 individus en bonne santé appariés pour l'âge et le sexe. (who.int)
- We wished to extend this study in a well-defined group of patients with major trauma, and to investigate potential mechanisms for rapid intrapulmonary IL-8 generation.Materials and methodsPatients with major trauma underwent bronchoalveolar lavage (BAL) and IL-8 levels were measured in BAL fluid by ELISA. (ox.ac.uk)
Analogues was observed1
- No significant cross-reactivity or interference between Rabbit IL-8 and analogues was observed. (caslab.com)
Stimulates1
- IL-8 also stimulates phagocytosis once they have arrived. (wikipedia.org)
Pathway3
- The interleukin-8 pathway in cancer. (hoelzel-biotech.com)
- Moreover, pretreatment with interleukin-8 (IL-8) completely abrogated the response to ABF, demonstrating desensitization of a common pathway. (ed.ac.uk)
- This pulmonary exposure to alcohol can damage the bronchial epithelial layer of the lung [ 6 , 7 ], while impairing the lung's ability to repair epithelial wounding via inhibition of the PKA pathway [ 8 ]. (cdc.gov)
Proliferation2
- Die Exkretion von IL - 8 aus Krebszellen, fördert die Proliferation und die Überlebensdauer von Krebszellen durch die Aktivierung von autokrinen Signalwegen. (hoelzel-biotech.com)
- Interleukin 2 (IL-2) promotes the proliferation of T cells. (fujifilm.com)
Proinflammatory1
- Acute hypoxia may represent one of potentially several proinflammatory stimuli responsible for rapid intrapulmonary IL-8 generation in patients at-risk of ARDS. (ox.ac.uk)
Receptors3
- IL-8 can be secreted by any cells with toll-like receptors that are involved in the innate immune response and has been demonstrated to be a signatory chemokine of CR2+ naive T cells, also known as recent thymic emigrants. (wikipedia.org)
- Both monomer and homodimer forms of IL-8 have been reported to be potent inducers of the chemokine receptors CXCR1 and CXCR2. (wikipedia.org)
- However, ABF was unable to fully desensitize the response to IL-8, and binding to CXCR1 or CXCR2 was excluded in experiments using RBL-2H3 cells transfected with the two human IL-8 receptors. (ed.ac.uk)
Humans2
- In humans, the interleukin-8 proteins encoded by the IL8 gene. (gentaur.com)
- This lack of translation from current in vitro and in vivo models into humans has led to challenges in drug discovery 8 . (nature.com)
Concentrations6
- Amniocentesis being an invasive procedure, we wanted to investigate whether elevated matrix metalloproteinase-8 (MMP-8) or interleukin-6 (IL-6) concentrations could be detected from cervical fluid samples. (helsinki.fi)
- Results: In women without PPROM, cervical MMP-8 concentrations correlated with AF MMP-8 concentrations (r(S) = 0.466, p = 0.002), but cervical IL-6 did not correlate with AF IL-6 (r(S) = 0.277, p = 0.076). (helsinki.fi)
- Women with MIAC had higher concentrations of AF MMP-8 and AF IL-6 compared to women without MIAC regardless of membrane status. (helsinki.fi)
- However, only women without PPROM had higher concentrations of cervical MMP-8 in proven MIAC. (helsinki.fi)
- IL-8 augmented the [Ca 2+ ](i) more efficiently in CXCR1-transfectants than in CXCR2-transfectants, whereas for ENA-78, threefold higher concentrations were necessary to obtain a calcium response in CXCR1-transfected cells than in CXCR2-transfectants. (tau.ac.il)
- The half-maximal effective concentrations for migration of CXCR2-transfectants in response to GCP-2 and ENA-78 were similar (0.1 nM), whereas GCP-2 was tenfold more potent than ENA-78 on CXCR1-transfectants, Half-maximal migration of CXCR1-transfected and CXCR2-transfected cells was obtained with IL-8 at concentrations of no more than 0.01 nM. (tau.ac.il)
Concentration4
- At a concentration of 1% (vol/vol), DMSO blocked IL-8 release by approximately 90% in the presence of 1 microgram/ml LPS at a 24-h time point, but did not affect cell viability or reduce the production of tumor necrosis factor (TNF), interleukin 6, or interleukin-1 beta (IL-1 beta). (jci.org)
- The OD value is proportional to the concentration of Rabbit IL-8. (caslab.com)
- You can calculate the concentration of Rabbit IL-8 in the samples by comparing the OD of the samples to the standard curve. (caslab.com)
- Conclusion: In women without PPROM, cervical MMP-8 concentration reflects the magnitude of AF MMP-8, thus potentially guiding the selection of patients benefitting from amniocentesis. (helsinki.fi)
Macrophage1
- We have previously demonstrated significantly raised interleukin-8 (IL-8) levels in the lungs of at-risk patients that progress to ARDS, and identified the alveolar macrophage as an important source of this chemokine. (ox.ac.uk)
Macrophages5
- Usually, it is the macrophages that see an antigen first, and thus are the first cells to release IL-8 to recruit other cells. (wikipedia.org)
- While neutrophil granulocytes are the primary target cells of IL-8, there are a relatively wide range of cells (endothelial cells, macrophages, mast cells, and keratinocytes) that respond to this chemokine. (wikipedia.org)
- The regulation of interleukin-8 by hypoxia in human macrophages--a potential role in the pathogenesis of the acute respiratory distress syndrome (ARDS). (ox.ac.uk)
- Acute hypoxia, a clinically relevant stimulus, rapidly and selectively upregulates IL-8 in macrophages associated with a novel pattern of transcription factor activation. (ox.ac.uk)
- The poultry PM-induced IL-8 release by the bone marrow-derived macrophages of cPLA2 knockout mice was significantly lower. (cdc.gov)
Inflammation4
- These results provide evidence that reactive oxygen metabolites play an important role in the regulation of IL-8 production and suggest that reduction of IL-8 release may contribute to the beneficial effects of antioxidants in experimental models of inflammation and ischemia/reperfusion injury. (jci.org)
- Inflammation is linked to IL-8 in a variety of illnesses. (walkinlab.com)
- A sufficient response of neutrophil granulocytes stimulated by interleukin (IL)-8 is vital during systemic inflammation, for example, in sepsis or severe trauma. (uni-ulm.de)
- Overall, we provide novel mechanistic insights for the interaction of IL-8 with neutrophil granulocytes and report in detail about its alteration during systemic inflammation. (uni-ulm.de)
Role in the pathogenesis1
- IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. (wikipedia.org)
CXCR13
- GCP-2 desensitized the calcium increase induced by IL-8 in both CXCR1-transfected and CXCR2-transfected cells, but ENA-78 only affected the IL-8-induced calcium response in CXCR2-transfectants. (tau.ac.il)
- In contrast, only IL-8 and GCP-2 but not ENA-78, competed for 122 5I-IL-8 binding to CXCR1. (tau.ac.il)
- From these data, it can be concluded that, in addition to IL-8, GCP-2, but not ENA-78, efficiently binds to both CXCR1 and CXCR2. (tau.ac.il)
Chemotaxis1
- IL - 8 aktiviert Neutrophile, welche ihrerseits die Chemotaxis, Exozytose und den oxidativen Burst induzieren. (hoelzel-biotech.com)
Peptide1
- IL-8 is initially produced as a precursor peptide of 99 amino acids which then undergoes cleavage to create several active IL-8 isoforms. (wikipedia.org)
Human4
- Oxygen radical scavengers selectively inhibit interleukin 8 production in human whole blood. (jci.org)
- scavenger dimethyl sulfoxide (DMSO) was found to dose-dependently inhibit interleukin 8 (IL-8) production in LPS-stimulated human whole blood. (jci.org)
- Carrier fish have been implicated in fish-to-fish transmission of S. iniae ( 8 ), and these carriers may be responsible for human infection because fish with overt signs of disease are unmarketable. (cdc.gov)
- previous analysis of vIL-6 activity.IMPORTANCE Human herpesvirus 8 (HHV-8) -encoded viral interleukin-6 (vIL-6) was the first viral IL-6 homologues to be identified. (ilgenetics.org)
CXCR21
- Radiolabeled IL-8 could efficiently be displaced from CXCR2 by IL-8, GCP-2 and ENA-78. (tau.ac.il)
Modulation1
- Up-modulation of IL-8 after P. aeruginosa infection was counteracted in IB3-1, CuFi-1, and NuLi-1 cells by pre-miR-93 transfection. (univr.it)
Coli1
- E. coli interleukin-8 for cell culture or antibody production. (gentaur.com)
Biochemical1
- Through a chain of biochemical reactions, IL-8 is secreted and is an important mediator of the immune reaction in the innate immune system response. (wikipedia.org)
Baseline1
- Cognition was measured at baseline and follow-up over 8 years. (lu.se)
Regulation1
- The possible involvement of miR-93 in IL-8 gene regulation was validated using three luciferase vectors, including one carrying the complete 3'-UTR region of the IL-8 mRNA and one carrying the same region with a mutated miR-93 site. (univr.it)
Significantly2
- When stimulated with Poly I:C, ATC/TTC haplotype, cells significantly up-regulated the IL-8 at both transcriptional and translational levels. (nih.gov)
- Janice C. Law, MD, and her colleagues at the Vanderbilt Eye Institute reported to the November 2008 annual AAO meeting that interleukin 6 (IL-6), was found to be significantly higher in a group of 57 AMD patients when compared to an age-matched control group. (preventblindness.org)
Cells7
- Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. (wikipedia.org)
- Two sgRNAs vectors targeting the IL-8 gene and the naked homologous repair DNA carrying different haplotypes were used to successfully generate HEK293T cells carrying the AT genotype at the first SNP - rs4073 (alias -251), TT genotype at the second SNP - rs2227307 (alias +396), TC or CC genotypes at the third SNP - rs2227306 (alias +781) at the IL-8 locus. (nih.gov)
- In addition, IL-8 was up-regulated in uninfected cells treated with antagomiR-93. (univr.it)
- Nuclear extracts from Jurkat cells infected with Corby or flaA mutant were mixed with IL-8 NF-κB probe (MOI, 100:1). (biomedcentral.com)
- Experimental and clinical evidence indicates that vIL-6 is important for the onset and / or progression of the pathology associated with HHV-8 B-cells, and endothelial, including AIDS-related Kaposi's sarcoma and multicentric Castleman's disease. (ilgenetics.org)
- Experiments were conducted to understand the mechanisms for C5a-induced IL-8 production, which was 14-fold greater than that in unstimulated cells by 2 hours. (elsevierpure.com)
- The poultry PM-induced cPLA2 serine phosphorylation and IL-8 release were attenuated by AACOCF3, PD98059, and by transfection with dominant-negative ERK-1/2 DNA in cells. (cdc.gov)
Potent1
- These results demonstrate for the first time that LPA is a potent stimulator of IL-8 production in HBEpCs, which involves PKCδ/NF-κB signaling pathways. (johnshopkins.edu)
Assay1
- Intercellular adhesion molecule 1 (ICAM-1), interleukin (IL)-8, and IL-6 levels in the supernatant of VSMCs were measured with enzyme-linked immunosorbent assay 0 and 24 h after grouping. (geneticsmr.com)
Production3
- Furthermore, this effect was not LPS-specific, in that IL-8 production was reduced by DMSO to a similar extent upon stimulation of blood with phytohemagglutinin, aggregated immune complexes, TNF, or IL-1 beta. (jci.org)
- Finally, incubation of tracheal aspirates with a neutralizing antibody against IL-1β reduced epithelial cell IL-8 production, whereas neutralizing antibodies against IL-1α and TNF-α had no effect. (utmb.edu)
- Furthermore, LPA-activated PKCδ and the LPA-mediated activation of NF-κB and IL-8 production were attenuated by overexpression of dominant-negative PKCδ and rottlerin. (johnshopkins.edu)