Interacts with microtubules microtubule associated proteins. Medical search. Frequent questions

APC also interacts with the actin cytoskeleton to regulate cell polarity. (ku.edu)
In an mutant trichomes, the organization of the actin cytoskeleton is normal but the distribution of microtubules is aberrant. (nih.gov)
A role of AN in the control of the microtubule cytoskeleton is further supported by the finding that AN genetically and physically interacts with ZWICHEL, a kinesin motor molecule involved in trichome branching. (nih.gov)
Our data suggest that CtBP/BARS-like protein function in plants is directly associated with the microtubule cytoskeleton. (nih.gov)
The molecular events dictating cellular division require the orchestration of numerous signaling and actin cytoskeleton interacting proteins. (upstate.edu)
Tau interacts with actin in the cytoskeleton and neuronal outgrowth, anchors enzymes such as protein kinases and phosphatases, and regulates intracellular vesicle transport. (biovendor.com)
Plant cytoskeleton consists of two major networks of protein polymers, actin microfilaments (AFs) and microtubules (MTs). (cas.cz)
Centrosomes nucleate the formation of the microtubule cytoskeleton in interphase cells and form the poles of the mitotic spindle during cell division. (elifesciences.org)
We focus on the microtubule cytoskeleton - the dynamic network of protein fibers that pulls the chromosomes apart at mitosis, acts as "railroad tracks" for intracellular transport, and organizes the cytoplasm. (nd.edu)
The chemi- appear to interact with the structural elements of the cell, cal analysis was assessed at DATA CHEM Laboratories using plasma- with apparent binding to the cytoskeleton [Porter et al. (cdc.gov)

In this review we will highlight mechanisms that control targeting of PSD-95 at the synapse, and discuss how this molecule influences the retention and clustering of diverse synaptic proteins to regulate synaptic structure and strength. (frontiersin.org)
Here, we show that MAP7, a less-well understood MAP that is localized to branch junctions, provides a key molecular mechanism to regulate microtubule stability during branch formation. (jefferson.edu)
Together, our study identifies a novel molecular mechanism mediated by MAP7 to regulate microtubule stability and strengthen branches at different stages of axonal branch morphogenesis. (jefferson.edu)
Microtubules also regulate paracellular permeability. (plos.org)

APC is implicated in spindle formation by direct interactions with tubulin and microtubule-binding protein EB1. (ku.edu)
Tau, a microtubule-associated protein expressed in mature neurons, interacts with tubulin to promote the assembly and stabilization of microtubules. (megazyme.com)

2021. Reducing the expression of the Numb adaptor protein in neurons increases the searching behavior of Drosophila larvae . (truman.edu)
A new study shows that when the gene Foxp2 is knocked out in mouse striatal neurons (top right panel), the protein dynactin (stained red) and the chain that binds dynactin and dynein (stained green) show abnormal spacing compared to wildtype neurons (top left panel). (medicalxpress.com)

MAP7 Prevents Axonal Branch Retraction by Creating a Stable Microtubule Boundary to Rescue Polymerization. (jefferson.edu)
Development and maintenance of axonal branches rely on microtubule stability, but the underlying molecular mechanisms are not fully understood. (jefferson.edu)
This unique binding property supports a novel mechanism mediated by MAP7 to cooperate with other MAPs and control microtubule stability during axonal branch development. (jefferson.edu)
Tau protein is highly soluble and normally attached to axonal microtubules. (biovendor.com)

Utilizing this APC-M2 pAb, we immunoprecipitated endogenous APC and its binding proteins from colon epithelial cells expressing wild-type APC. (ku.edu)
In contrast, overexpression of AQP5 increased assembly of microtubules, with evidence of increased MT stability, and promoted the formation of long straight microtubules in the apical domain of the epithelial cells. (plos.org)
The nanotube bundles are similar to the potential of nanotubes to induce genetic damage size of microtubules that form the mitotic spindle in normal lung cells, cultured primary and immor- and may be incorporated into the mitotic spindle talized human airway epithelial cells were apparatus. (cdc.gov)

The kinetochore is a complex macromolecular machine that hierarchically assembles from a set of conserved multi-protein complexes ( Figure 1 ). (uni-due.de)
This technique allows the observation of individual kinetochore complexes and microtubule-binding proteins with single-molecule sensitivity to reveal their mode of interaction with dynamic plus-ends. (uni-due.de)
This suggests that the functions of the motor complexes formed by these proteins may be impaired. (medicalxpress.com)
Here we summarize recent advances in our knowledge about the candidate proteins or protein complexes that interact with both AFs and MTs and their role in fundamental cellular and developmental processes. (cas.cz)

When a cell divides into two, its microtubules must properly capture each chromosome at specialised multi-protein structures called kinetochores. (qmul.ac.uk)
Therefore, to understand how aneuploidy arises, we should understand how kinetochores are captured by microtubules. (qmul.ac.uk)
Recent high-resolution microscopy work in the Draviam group showed that kinetochores are first captured along microtubule walls and then brought to microtubule-ends. (qmul.ac.uk)
A defining feature of kinetochores is their ability to interact with microtubule plus-ends through multiple rounds of polymerization and depolymerization. (uni-due.de)
Kinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. (anl.gov)

We also present evidence for interactions between APC and IFs, independent of actin filaments and microtubules. (ku.edu)
I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
Besides the more or less indirect cooperation between AFs and MTs, their direct interactions through specific physically interacting proteins has been well described in yeast, nematodes, insect and animal cells. (cas.cz)
Recently, promising candidates for corresponding homologous proteins have been identified in plants, although there is still lack of functional evidence for these interactions. (cas.cz)
To identify proteins interacting with alpha A-crystallin (CRYAA) and to investigate the potential role that these protein interactions play in the function of CRYAA using a human proteome (HuProt) microarray. (molvis.org)
A HuProt microarray composed of 17,225 human full-length proteins with N-terminal glutathione S-transferase (GST) tags was used to identify protein-protein interactions. (molvis.org)
The protein-protein interactions may help CRYAA carry out multifaceted functions. (molvis.org)
To understand the functions of CRYAA and how these are regulated, protein-protein interactions need to be investigated. (molvis.org)
Goodson, H. V. and Jonasson, E. M. "Teaching Protein-Ligand Interactions using a Case Study on Tau in Alzheimer's Disease" 2022 Journal of Chemical Education , 99 (8), pp.3064-3067. (nd.edu)
We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. (anl.gov)

The similarity of carbon nanotube s to microtubules suggests a potential to interact with cellular biomolecules, such as the mitotic spindle, as well as the motor proteins that separate the chromosomes during cell division. (cdc.gov)
Our results are the first to tubes (SWCNT) suggest the potential to interact report disruption of the mitotic spindle by with critical biological structures. (cdc.gov)

One of these molecular motors is the dynein protein complex, a large cluster of proteins that is responsible for shuttling molecules along microtubule scaffolds within cells. (medicalxpress.com)
The dynein complex is made up of several other proteins. (mit.edu)
The most important of these is a protein called dynactin1, which interacts with microtubules, enabling the dynein motor to move along microtubules. (mit.edu)

This analysis has already yielded some important insights: For example, the Dam1 complex, a specialized microtubule-binding component of the budding yeast kinetochore oligomerizes to form a ring around microtubules in vitro ( Figure 2 ). (uni-due.de)
To analyze this process we have reconstituted dynamic microtubules in vitro and visualized the interaction of individual kinetochore components using total internal reflection fluorescence (TIRF) microscopy (Movie 1) . (uni-due.de)
Dynamic Microtubules (red) growing in vitro from a Microtubule-organizing center (MTOC). (uni-due.de)
The C-terminal domain of KAC1 could interact with F-actin in vitro. (scite.ai)

To answer these questions we use a combination of biochemistry, molecular biology, quantitative microscopy, and (in collaboration with applied mathematician Mark Alber) computational models of microtubule dynamics. (nd.edu)
Zanic, M. and Goodson, H. V. "Quantification of Microtubule Stutters: Dynamic Instability Behaviors that are Strongly Associated with Catastrophe" 2022 Molecular Biology of the Cell , 33 (3), ar22. (nd.edu)

This function is mediated by the ability of MAP7 to control microtubule stability, as microtubules are more stable at branch junctions where MAP7 is localized. (jefferson.edu)
Here, we show that MAP7, a unique protein that interacts with both microtubules and the motor protein kinesin-1, plays a key role at branch junctions. (jefferson.edu)
Many of these junctions lie in or near "unknown" or "hypothetical" proteins. (purdue.edu)

This is an important cellular event because when the kinetochore is not tethered to microtubule-ends, the growth and shrinkage of microtubule-ends can not push or pull chromosomes. (qmul.ac.uk)
1-15 months: The student will generate mutants of outer-kinetochore proteins that interact with microtubules. (qmul.ac.uk)
15-30 months: The student will choose mutant phenotypes from year-1 for live-cell analysis for analysing changes in microtubule-mediated pulling of kinetochore and chromosome-microtubule attachment status. (qmul.ac.uk)
Our investigations into kinetochore assembly have led to the identification of a conserved receptor molecule for the microtubule-binding Ndc80 complex. (uni-due.de)
We have recently started to investigate motor proteins involved in kinetochore transport ( Movie 2 ). (uni-due.de)

We have solved the crystal structure of the interface between Ndc80 and the histone-fold protein Cnn1 and we are further investigating how the cell employs different Ndc80 receptors to promote chromosome segregation. (uni-due.de)
3.0: hematopoietic cell-specific Lyn substrate 1 (HCLS1), Kelch domain-containing 6 (KLHDC6), sarcoglycan delta (SGCD), KIAA1706 protein (KIAA1706), RNA guanylyltransferase and 5′-phosphatase (RNGTT), chromosome 10 open reading frame 57 (C10orf57), chromosome 9 open reading frame 52 (C9orf52), and plasminogen activator, urokinase receptor (PLAUR). (molvis.org)
In humans, the CRYAA gene encodes a 173 amino acid residue protein by single copy genes located on chromosome 21. (molvis.org)

During development, maturation of nascent branches depends on stabilization of newly assembled or transported microtubules, which are thought to be regulated by microtubule-associated proteins (MAPs). (jefferson.edu)

Fernandes, G. F. and Goodson, H. V. "Overexpression of the Microtubule-Binding Protein CLIP-170 Induces a Plus TIP Network Superstructure Consistent with a Biomolecular Condensate" 2021 PLOS One , 16 (12), e0260401. (nd.edu)

KAC motor domains are similar to those of the kinesin-14 subfamily (such as Ncd and Kar3) but do not have detectable microtubule-binding activity. (scite.ai)

Moreover, MAP7 binds to the acetylated and stable region of individual microtubules and avoids the dynamic plus end, thereby creating a boundary that prevents microtubule depolymerization and rescues microtubule polymerization. (jefferson.edu)
This unique binding property, which is not observed for other MAPs, can prevent branch retraction caused by laser-induced severing or nocodazole-induced microtubule depolymerization. (jefferson.edu)
MAP7 also binds to the acetylated region of microtubules to prevent depolymerization and rescue polymerization. (jefferson.edu)

In Arabidopsis thaliana, phototropins mediate chloroplast movement by regulating short actin filaments on chloroplasts (cp-actin filaments), and the chloroplast outer envelope protein CHUP1 is necessary for cp-actin filament accumulation. (scite.ai)

Negative stain electron microscopy of Dam1 complex decorating microtubules. (uni-due.de)
Single Cik1-Kar3 Kinesin-14 motor proteins (red) moving along taxol-stabilized microtubules (blue), visualized by TIRF microscopy. (uni-due.de)

These experiments support the hypothesis that the mechanism for the establishment of cleavage furrows is the same for all types of cells and that the centrosomes and their linearly connected microtubules may play an important role for the assembly of the cleavage furrow. (upstate.edu)

Here we show that the AN gene encodes a C-terminal binding proteins/brefeldin A ADP-ribosylated substrates (CtBP/BARS) related protein. (nih.gov)

Here, we report that AQP5 promotes microtubule assembly and helps maintain the assembled microtubule steady state levels with slower turnover dynamics in cells . (plos.org)
These findings indicate that AQP5-mediated regulation of microtubule dynamics modulates airway epithelial barrier properties and epithelial function. (plos.org)

We hope not only to provide a mechanistic understanding of this process but also to explore more generally the type of features that allow translocation of molecules along microtubules. (uni-due.de)
Note unidirectional, processive runs of individual motor molecules along the microtubules. (uni-due.de)
Those malfunctions arise because Foxp2 mutations prevent the proper assembly of motor proteins, which move molecules within cells, the researchers found. (medicalxpress.com)

MAP7 stabilizes microtubules in nascent branches and prevents branch retraction during branch maturation or after laser-induced injury. (jefferson.edu)
Tau stabilizes the microtubules and makes them rigid. (biovendor.com)

This mechanism could also impact microtubule regulation in branch regeneration after nerve injury. (jefferson.edu)
A proteomic analysis of KO oocytes revealed the down-regulation of proteins associated with microtubules including tubulins, which occurred independently of transcriptomic changes. (life-science-alliance.org)

however, the long thin tubular-shaped carbon nanotube s have a striking similarity to cellular microtubules. (cdc.gov)

However, because many known MAPs inhibit branch formation, it is not clear which MAP is responsible for regulating microtubule stability during branch development. (jefferson.edu)

In the new study, led by Liu, the researchers investigated one proposed mechanism, which is that Foxp2 affects motor proteins. (medicalxpress.com)

I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
I published the first report of plant ER reticulon protein interactors by Co-IP and FRET-FLIM. (brookes.ac.uk)

Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS), we identified 42 proteins in complex with APC, including β-catenin and intermediate filament (IF) proteins lamin B1 and keratin 81. (ku.edu)
Although inhibitory postsynaptic sites lack PSDs, they also consist of complex protein matrices. (frontiersin.org)

Jonasson, E. M. and Goodson, H. V. "The CLIP-170 N-Terminal Domain Binds Directly to both F-Actin and Microtubules in a Mutually Exclusive Manner" 2022 Journal of Biological Chemistry , 298 (5), 101820. (nd.edu)

Functional annotation clustering showed that they belong to cell cycle, organelle or nuclear lumen, protein transport, and DNA binding and repair clusters. (molvis.org)
By performing cell biological and biochemical experiments using human cells, Moyer and Holland show that PLK4 interacts with a protein called STIL that is found in the central part of the centriole. (elifesciences.org)
How do other parts of the cell (organelles, chromosomes, the cell cortex) interact with microtubules? (nd.edu)
Shah, M. A. and Giannakakou, P. "CLIP-170S is a Microtubule Plus TIP Variant that Confers Resistance to Taxanes by Impairing Drug-Target Engagement" 2021 Developmental Cell , 56 (23), pp.3264. (nd.edu)

Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)
The identified proteins were subjected to subsequent bioinformatics analysis using the DAVID database. (molvis.org)

CRYAA interacted with these proteins to maintain their solubility and decrease the accumulation of denatured target proteins. (molvis.org)

I took up a position at Oxford Brookes University in 2012 investigating the role of reticulon proteins in ER tubulation and viral trafficking in order to develop my international reputation in ER research and advanced imaging. (brookes.ac.uk)

The cleavage furrow is the site of actin, actin-interacting proteins, septins and microtubule assembly into a contractile ring, and is regulated by numerous signaling proteins, such as Rho. (upstate.edu)
We propose that procentriole assembly requires PLK4 to phosphorylate STIL in two different regions: phosphorylation of residues in the STAN motif allow STIL to bind SAS6 and initiate cartwheel assembly, while phosphorylation of S428 promotes the binding of STIL to CPAP, linking the cartwheel to microtubules of the centriole wall. (elifesciences.org)

We have performed microinjection studies and monitored the activity of numerous live fluorescently labeled probes to address protein incorporation during cytokinesis. (upstate.edu)

While establishing the history of protein families is an important goal in itself, our primary interest has been in using the history of a protein family to help understand how its members work now. (nd.edu)

The foamy stromal cells between the capillaries stain positive for glial fibrillary acid protein and neuron-specific enolase. (medscape.com)
When cells are about to divide, they make a new set of centrioles by assembling a variety of proteins. (elifesciences.org)
The modification of STIL at a specific location by PLK4 was needed to link it to another protein in the outer wall of the centriole, and was also necessary for the cells to build new centrioles. (elifesciences.org)
Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a protein essential for the maintenance of DNA methylation in somatic cells. (life-science-alliance.org)

Here, we report that two kinesin-like proteins, KAC1 and KAC2, are essential for chloroplasts to move and anchor to the plasma membrane. (scite.ai)

Consistently, nascent branches depleted of MAP7 have decreased stable microtubules but increased dynamic microtubules. (jefferson.edu)

Tau is a microtubule-associated protein found predominantly in neuronal axons of the vertebrate brain. (biovendor.com)

The presence of cp-actin filaments depends on an actin-binding protein, chloroplast unusual positioning1 (CHUP1), localized on the chloroplast envelope. (scite.ai)

The HuProt microarray results showed that the signals of 343 proteins were higher in the recombinant CRYAA group than in the control group. (molvis.org)

The growing plus-ends of microtubules are decorated with EB1 (green). (uni-due.de)
Topics of particular interest include microtubule plus-end tracking proteins (+TIPs), a network of diverse proteins that dynamically track growing microtubule plus ends, as well as the disease-associated proteins Tau (Alzheimer's) and stathmin (cancer). (nd.edu)

Ochi T et al PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair Science 2015 347 (6218): 185-188. (qmul.ac.uk)

One-hundred and twenty-seven of 17,225 human full-length proteins were identified that interact with CRYAA. (molvis.org)

Anatomy48

Organisms29

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes51

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science5

Health Care2

Cytoskeleton10

Regulate4

Tubulin2

Neurons2

Axonal4

Epithelial cells3

Complexes4

Kinetochores5

Interactions11

Mitotic2

Dynein3

Vitro4

Molecular2

Junctions3

Kinetochore5

Chromosome3

Stabilization1

Overexpression1

Detectable1

Depolymerization3

Arabidopsis1

Microscopy2

Centrosomes1

Gene encodes1

Dynamics2

Molecules3

Stabilizes2

Regulation2

Cellular1

Stability1

Motor1

Plant2

Complex2

20221

Cell4

Bioinformatics2

Accumulation1

Role1

Assembly2

Probes1

Important1

Cells4

Move1

Dynamic1

Found1

Depends1

Control1

Plus-ends2

Promote1

Human1