• It is thought that (p)ppGpp may affect the stability of the open complex formed by RNA polymerase on DNA and therefore affect promoter clearance. (wikipedia.org)
  • Noteworthy, was our demonstration that although termination of DNA replication did not require specific DNA sequences, some DNA sequences did promote pausing of DNA replication forks in vivo (and DNA polymerase in vitro), and some sequences, such as thosewithin the termination region for SV40 DNA replication, did promote formation of catenated intertwines during separation of sibling chromosomes. (nih.gov)
  • DNA polymerase has a significant role in synthesizing new DNA by incorporating dNTPs into the growing strand. (geneticeducation.co.in)
  • The DNA polymerase incorporates nucleotides when dsDNA unwinds and becomes single-stranded. (geneticeducation.co.in)
  • The in vivo process of copying or synthesizing the DNA using the DNA polymerase is known as replication. (geneticeducation.co.in)
  • This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
  • First-line and second-line antiviral drugs used to treat HSV infections, acyclovir and related compounds, as well as foscarnet and cidofovir, selectively inhibit herpesvirus DNA polymerase (DNA-pol). (bvsalud.org)
  • The discovery of anti-herpesvirus agents that affect biotargets other than DNA polymerase will expand our possibilities of targeting HSV infections, including those resistant to baseline drugs. (bvsalud.org)
  • RESULTS: We expressed the gene encoding Enterococcus faecium phage IME199 DNA polymerase (IME199 DNAP) in Escherichia coli BL21(DE3), and characterized protein function. (bvsalud.org)
  • In addition, IME199 DNAP has dNTP-dependent 5'-3' polymerase activity and can amplify DNA at 15-35 °C and a pH range of 5.5-9.5. (bvsalud.org)
  • CONCLUSIONS: A novel family B DNA polymerase was successfully overproduced in Escherichia coli BL21(DE3). (bvsalud.org)
  • Based on the characterized properties, IME199 DNAP is expected to be developed as a high-fidelity polymerase for DNA amplification at room temperature. (bvsalud.org)
  • Functionally active DNA sequences are genes having a role in making proteins. (geneticeducation.co.in)
  • Chromosome replication is triggered when a nucleoprotein complex, termed the orisome, assembles, unwinds the duplex DNA, and recruits the proteins required to establish new replication forks. (mdpi.com)
  • Escherichia coli is a host bacterium widely used for the production of recombinant proteins, is the cell factory of choice for in vitro studies of phage protein function. (bvsalud.org)
  • In a recent study posted to the bioRxiv * pre-print server, researchers performed high-throughput evolutionary analyses of genes encoding 334 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral-interacting proteins (VIPs) of modern humans, primates, and bats, followed by comprehensive functional-genetic analyses of seven VIPs of interest. (trendfool.com)
  • We also used Mass-Spec Affinity purification to identify the host proteins interacting with SARS-CoV-2 Orf6 protein and tested a drug that could inhibit a specific Orf6 and host protein interaction. (biomedcentral.com)
  • Proteomics revealed Orf6 interacts with nucleopore proteins (RAE1, XPO1, RANBP2 and nucleoporins). (biomedcentral.com)
  • Our study revealed Orf6 as a highly pathogenic protein from the SARS-CoV-2 genome, identified its key host interacting proteins, and Selinexor as a drug candidate for directly targeting Orf6 host protein interaction that leads to cytotoxicity. (biomedcentral.com)
  • For example, HIV-1 Vpr protein interacts with host proteins to modify host cell energy metabolism, oxidative status and proteasome function. (biomedcentral.com)
  • Modest ppGpp levels inhibit pR and active pE, pI, and paQ promoters in vivo and have effects in vitro that seem to favor lysogeny. (wikipedia.org)
  • Obg protein interacts with several regulators (RsbT, RsbW, RsbX) necessary for the stress activation of sigma B. The (p)ppGpp levels of the host seem to act as a sensor for phage lambda development, primarily affecting transcription. (wikipedia.org)
  • Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)
  • BACKGROUND: Identification and characterization of novel, faithful and processive DNA polymerases is a driving force in the development of DNA amplification methods. (bvsalud.org)
  • We demonstrated that DNA synthesis occurs discontinuously only on one arm of replication forks (the arm where the direction of synthesis is opposite to the direction of fork movement) through the repeated initiation, synthesis and joining of Okazaki fragments (transient nascent DNA chains of 40 to 300 nucleotides). (nih.gov)
  • The process of synthesis of DNA. (geneticeducation.co.in)
  • The amino acid residues Asp30, Glu32, Asp112 and Asp251 are the 3'-5' exonuclease active sites of IME199 DNAP, while residues Asp596 and Tyr639 are essential for DNA synthesis by IME199 DNAP. (bvsalud.org)
  • Y-family DNA polymerases can bypass DNA lesions, which may result from external or internal DNA damaging agents, including some chemotherapy agents. (bvsalud.org)
  • In E. coli amino acid starvation inhibited DNA replication at the initiation stage at oriC, most probably owing to the lack of the DnaA replication initiation protein. (wikipedia.org)
  • His DNA displaces myotubes for leading metal and secretion kidney, controlling processive helicases, and limiting the mitochondria that activate the loading activation. (erik-mill.de)
  • DNA is double-stranded, we all know, in this mode of replication, when two strands separate, each work as a template for replication to occur. (geneticeducation.co.in)
  • Several in vitro approaches, such as clustered regularly interspaced short palindromic repeats (CRISPR)/gene knockout (KO) screens, complementary DNA (cDNA) library screens, or mass spectrometry (MS) analyses, have helped researchers identify hundreds of SARS-CoV-2 VIPs. (trendfool.com)
  • Though this data supplies varied helpful outcomes, it is usually essential to conduct in vitro research to grasp optimum remedy approaches. (aidstar-one.com)
  • My laboratory has developed new technologies and applied them towards understanding the molecular biology and enzymology of DNA replication in animal cells and viruses (SV40, polyomavirus, papillomavirus, and herpes simplex virus), and at the beginning of animal development (mouse preimplantation embryos and frog eggs). (nih.gov)
  • In fact, amplification of genes by over replication of certain regions of DNA is one of the primary mechanisms by which cancer cells become resistant to drug therapy. (nih.gov)
  • Results from our lab, as well as from other labs, led to the identification of all of the various DNA replication intermediates in SV40 replication. (nih.gov)
  • A complex network of DNA and protein. (geneticeducation.co.in)
  • Papillomaviruses undergo a complex life cycle requiring regulated DNA replication. (biomedcentral.com)
  • pUL15, which is directly involved in the processing and packaging of viral DNA, is one of the crucial components of the HSV terminase complex. (bvsalud.org)
  • These VIPs, including transmembrane serine protease 2 (TMPRSS2), receptor-interacting serine/threonine-protein kinase 1 (RIPK1), FYVE and coiled-coil domain autophagy adaptor 1 (FYCO1), zinc finger protein 318 (ZNF318), and the prim-pol primase complex, most likely contributed to SARS-CoV pathogenicity in different mammalian hosts. (trendfool.com)
  • Extracellular vesicle DNA from human melanoma tissues contains cancer-specific mutations. (gu.se)
  • Mechanical removal of biofilm on titanium discs: An in vitro study. (gu.se)
  • Drugs that block DNA replication can arrest the spread of cancer cells and eliminate viral pathogens. (nih.gov)
  • What Drugs, Substances, or Supplements Interact with Fludara? (rxlist.com)
  • DNA replication is the primary event that regulates cellular and viral proliferation. (nih.gov)
  • We were among the first to develop and exploit sub-cellular systems that allowed SV40 and polyomavirus to complete DNA replication in vitro. (nih.gov)
  • This E1 phosphate map will provide a new tool to more fully understand viral replication and serve as a useful model for investigating regulation of viral and cellular DNA replication. (biomedcentral.com)
  • DNA damage and repair have been widely studied in relation to cancer and therapeutics. (bvsalud.org)
  • The DNA is a double-helix structure with a phosphate backbone, sugar and nitrogenous bases, and is known as deoxyribonucleic acid. (geneticeducation.co.in)
  • Voltage-gated K+ GPCRs( Kv) retrofit the cleavage of kinase, vitro and hematopoietic structure compartments. (erik-mill.de)
  • DNA primase (DnaG) was directly inhibited by (p)ppGpp. (wikipedia.org)
  • Our current research now focuses on two basic, interrelated questions: (1) How do mammalian cells decide where and when to initiate DNA replication? (nih.gov)
  • In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
  • Therefore, the overall goal of our work is to discover how DNA replication is regulated both in the large chromosomes of cells and in the "mini-chromosomes" of viruses and small extrachromosomal DNA molecules. (nih.gov)
  • We found that the Omicron mutation does not significantly impact the lauruside binding and that L02 connects more stably with respect to L01 in the complexes from both variants, even though both compounds prevalently interact within the same binding pocket. (cnr.it)
  • As an alkylating agent, the mechanism of action of the active metabolites may involve cross-linking of deoxyribonucleic acid (DNA), which may interfere with the growth of normal and neoplastic cells. (medscape.com)
  • In vitro tracheary element transdifferentiation of Col-0 suspension cells. (or.jp)
  • Cbl possibly is binding missense via it has amount with an re-uptake Dopamine, CIN85( Cbl-interacting review of 85kDa). (erik-mill.de)
  • 2) What are the requirements for DNA replication and transcription at the beginning of mammalian development? (nih.gov)
  • As a post-doctoral fellow with Paul Berg, I developed a subcellular system that allowed simian virus 40 (SV40) DNA to continue replication in vitro. (nih.gov)
  • Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) interacts with CDK2AP2, modulates the actions of transforming growth factor-B1, cyclin-dependent kinase 2 and retinoblastoma protein, and closely interacts with micro-RNA21 and micro-RNA25. (cancerindex.org)