At the start of the S-phase until late mitosis, geminin inhibits the replication factor Cdt1, preventing the assembly of the pre-replication complex. (wikipedia.org)
Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. (prospecbio.com)
A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition. (nii.res.in)
Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
2013. Geminin deploys multiple mechanisms to regulate Cdt1 before cell division thus ensuring the proper execution of DNA replication. (virosin.org)
The FUCCI program was originally created to indicate specific cell-cycle stages with a distinctive fluorescent signature within a spatiotemporal way using differentially shaded fluorescent tags on two cell-cycle-regulated proteins, that are Geminin and Cdt1 [27]. (scienceexhibitions.org)
MCM loss' zygotes were generated by preventing the factor geminin from being degraded as the presence of geminin stops Cdt1 directed loading of MCM during G1 phase (6). (biologists.com)
GMNN2
Geminin, DNA replication inhibitor, also known as GMNN, is a protein in humans encoded by the GMNN gene. (wikipedia.org)
In the present study, the DNA replication inhibitor Geminin (GMNN) was identified to be a direct target of hcmv-miR-US5-1. (virosin.org)
Inhibitor3
Geminin was originally identified as an inhibitor of DNA replication and substrate of the anaphase-promoting complex. (wikipedia.org)
In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. (prospecbio.com)
Geminin also inhibits the chromatin remodeling things Brahma and Brg1 to retain an undifferentiated state and acts being a transcription repressor or corepressor. (gabasignaling.com)
Inhibition4
In many cancer cell lines, inhibition of geminin by RNA interference results in re-replication of portions of the genome, which leads to aneuploidy. (wikipedia.org)
When the interaction between geminin and SWI/SNF is eliminated, geminin's inhibition to this process is eliminated and neural precursors are allowed to differentiate. (wikipedia.org)
Inhibition of Geminin during the "S phase" (by RNAi) results in an additional round of replication of portions of the genome. (prospecbio.com)
2003. Human cytomegalovirus infection leads to accumulation of geminin and inhibition of the licensing of cellular DNA replication. (virosin.org)
Chromatin5
Geminin promotes early neural fate commitment by hyperacetylating chromatin. (wikipedia.org)
Geminin has also been shown to interact with the SWI/SNF chromatin remodeling complex. (wikipedia.org)
Using chromatin conformation capture (Hi-C), chromosomes were shown to be organised into topological associated domains (TADs) reflecting DNA regions which prefer to interact with each other (intradomain) (1). (biologists.com)
However, the genomic landscape however is not sparse, with numerous factors occupying and interacting with the chromatin, all of which could prevent loop extrusion machinery movement. (biologists.com)
A member of the GEMC1 family, GEMC1 is highly phosphorylated and interacts with chromatin during formation of the pre-replication complex. (mc1-r.com)
Differentiation2
In neural precursor cells, high levels of geminin prevent terminal differentiation. (wikipedia.org)
The dual roles of geminin during trophoblast proliferation and differentiation. (virosin.org)
Mitosis1
Geminin levels drop at the metaphase-anaphase transition of mitosis when it is degraded by the anaphase-promoting complex. (wikipedia.org)
Roles2
A nuclear protein present in most eukaryotes and highly conserved across species, numerous functions have been elucidated for geminin including roles in metazoan cell cycle, cellular proliferation, cell lineage commitment, and neural differentiation. (wikipedia.org)
2012. Geminin is required for mitotic proliferation of spermatogonia. (virosin.org)
Proteins1
Grant and co-workers enhanced the recognition of difference 1 (G1)/synthesis (S) and S/G2 transitions utilizing the proliferating cell nuclear antigen (PCNA)-interacting proteins (PIP)-FUCCI build [28]. (scienceexhibitions.org)
Genes1
this model is currently known as 'loop extrusion' and has been shown to regulate gene expression by allowing distant genes to interact with each other within TADs (4,5). (biologists.com)
Neural2
Coincidentally, geminin was also shown to expand the neural plate in the developing Xenopus embryo. (wikipedia.org)
Ultimately, geminin allows cells uncommitted to any particular lineage to acquire neural characteristics. (wikipedia.org)
Interaction2
We previously demon strated that Scmh1 mediates a molecular interaction of PcG com plex one with geminin and that PcG complex one acts as an selleck chemicals E3 ubiquitin ligase for geminin to sustain the hematopoietic stem cell exercise. (gabasignaling.com)
The C terminal putative nuclear localization signal domain acts also as an interaction do primary for geminin. (gabasignaling.com)
Cell6
Geminin is absent during G1 phase and accumulates through S, G2 phase and M phases of the cell cycle. (wikipedia.org)
In these cell lines, geminin knockdown leads to markedly slowed growth and apoptosis within several days. (wikipedia.org)
Since geminin knockdown leads to cell death in many cancer cell lines but not primary cell lines, it has been proposed as a potential therapeutic target for cancer treatment. (wikipedia.org)
Geminin, therefore, is an important player in ensuring that exactly one round of replication occurs during each cell cycle. (wikipedia.org)
Geminin has been found to be overexpressed in several malignancies and cancer cell lines, while there is data demonstrating that geminin acts as a tumor suppressor by safeguarding genome stability. (wikipedia.org)
Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E. coli. (prospecbio.com)
Acts1
Understanding the connection between the atmosphere and Io's volcanic activity seems to be the key, along with how Io's atmosphere interacts with Jupiter's powerful magnetosphere, which acts as a sink for gases in the atmosphere. (gishbartimes.org)
Study1
Model organisms have been used in the study of geminin function. (wikipedia.org)
Made1
Winklevoss twins backed Geminin exchange has already made deals with New York authorities. (koinalert.com)
In addition metazoa have evolved a further mechanism of defence in the recently identified endogenous negative regulator of DNA replication, geminin. (medscape.com)
Replication inhibitor1
Geminin, DNA replication inhibitor, also known as GMNN, is a protein in humans encoded by the GMNN gene. (wikipedia.org)
Genomic2
In addition, increased genomic instability and tumorigenesis have been observed in geminin knockout mice in both the colon and lung. (wikipedia.org)
[ 12 ] Geminin is a protein expressed during S-G2-M with the function of inhibiting re-licensing and DNA replication after cell division has occurred in order to ensure genomic stability. (medscape.com)
Complex2
Geminin was originally identified as an inhibitor of DNA replication and substrate of the anaphase-promoting complex. (wikipedia.org)
These phosphorylations inhibit the ability of MCM and ORC complexes to interact with other members of the licensing complex. (nih.gov)
Cell death1
Since geminin knockdown leads to cell death in many cancer cell lines but not primary cell lines, it has been proposed as a potential therapeutic target for cancer treatment. (wikipedia.org)
Cells1
Immunoperoxidase for MCM2, geminin and Ki67 was performed and the percentage of positive cells counted. (medscape.com)