Interactions between the analytes. Medical search. Frequent questions

Both interdigitated electrode and single-gap systems can be arranged in parallel to allow for the detection of multiple analytes by one device. (wikipedia.org)
Surface plasmon resonance imaging (SPRi) brings label-free detection of molecular interactions to microarray analysis. (genengnews.com)
Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. (nature.com)
Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
When coupled with the breadth of existing global expertise in immunoassays, it is easy to see why ELISA still remains the methodology of choice by those labs tasked with analyte detection. (ddw-online.com)
These systems help in the detection of analytes and the screening of immunological components based on biomolecular interactions. (imarcgroup.com)
Electrochemical sensors make use of electrodes as a transducer element to convert chemical signals attributing to a target analyte into electrical signals for detection. (stanford.edu)
Continuous detection of the analyte by some suitable detection technique (absorbance, electrode potential etc. (lu.se)

This technique is based on the dispersion of a solid sorbent in liquid samples in the extraction isolation and clean-up of different analytes from complex matrices. (hindawi.com)
This technique has allowed the development of alternative methodologies such as dispersive solid phase extraction (DSPE), which is based on the addition of a sorbent directly into the analytical solution followed by dispersion favoring the contact between the sorbent and the analytes [ 14 , 15 ]. (hindawi.com)
Once the dispersion process is completed, the sorbent, with the analytes retained on its surface, is separated by a mechanical process, such as centrifugation or filtration. (hindawi.com)
The non-polar sorbent is endcapped (EC), reducing polar secondary interactions associated with surface silanol groups. (thomassci.com)
Problems with quantitation in solid-phase extraction (SPE) may be associated with an inability to recover target analytes or internal standards from the extraction sorbent. (chromatographyonline.com)
The choice of sorbent chemistry is very important, and, to achieve the optimum extraction selectivity, one needs to choose a sorbent based on the analyte and matrix component chemistry. (chromatographyonline.com)
The strength of electrostatic interactions between analytes and sorbent, some 15 times stronger than simple hydrophobic interactions, means that sorbents containing ionâ exchange moieties are likely to give the most selective extraction under most circumstances. (chromatographyonline.com)
Of course, although the ability of the sorbent to retain the analyte is of great importance, one also needs to consider the requirement to selectively elute the analyte, which again requires knowledge of the analyte chemistry and the interactions with the sorbent. (chromatographyonline.com)
To ensure optimum analyte retention, one should consider both the sample diluent and the sorbent equilibration solvent. (chromatographyonline.com)
It may be necessary to include a "soak" time, in which the flow is stopped and the sample remains stationary within the sorbent bed to maximize analyte retention and prevent poor analyte recovery. (chromatographyonline.com)
Wash solvents, used to selectively elute interferences from the sorbent while retaining the analyte, should be strong enough to obtain the cleanest extract possible. (chromatographyonline.com)

Understanding the interactions between your analyte and the column stationary phase is invaluable at all stages of GC method development as this interaction is what governs the degree of retention and separation of that analyte. (chromacademy.com)
It is essential that the mobile phase always contains at least 5% water as that water merges with the stationary phase and increases the stationary phase's affinity to the analyte. (thermofisher.com)
The strength of compound-stationary phase interaction determines an analyte's retention time. (sigmaaldrich.com)
The separation was accomplished under normal-phase conditions using a mobile phase composed of dichloromethane:isopropyl alcohol:trifluoroacetic acid:diethylamine (90:10:0.3:0.05 v/v/v/v) under isocratic mode of elution at 15 °C. The interactions between the analytes, additives, and the stationary phase were discussed and the most probable separation mechanism was proposed. (springer.com)

Box-Behnken design was exploited to evaluate the main and interaction effects of the selected critical process parameters on the critical quality attributes, viz resolution between thee peak pairs and retention time of last eluting peak. (ijpsonline.com)
It is useful for fast separations in bioanalytical applications due to the increased retention of early eluting analytes such as dipolar, unsaturated and conjugated analytes. (shimadzu.com)
Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation mode that can be used to improve the retention of challenging polar analytes. (shimadzu.com)
Similarly, the pH of the equilibration solvent should be adjusted to ensure that any weak ion-exchange ligands are in the charged form (the pKa of weak ionâ exchange sorbents can be obtained from the manufacturer's literature), to promote efficient retention of the target analytes. (chromatographyonline.com)
Further, one should consider loading the sample at a lower flow rate than is usual, because of the point-to-point nature of the electrostatic interactions in ion exchange, which require some degree of molecular orientation to occur, and therefore are inherently slower than other retention mechanisms. (chromatographyonline.com)

A microfluidic-free design allows for unprecedented time and cost savings for binding kinetics, affinity and analyte concentration measurement with high precision, accuracy and a broad dynamic range. (sartorius.com)
the amount of which is dependent upon the analyte concentration in the sample. (cdc.gov)
Immunoassay (IA) is a technique used to measure the concentration of analytes in a solution with an antibody or an antigen as a biorecognition agent. (imarcgroup.com)

Analytes: reversed phase, aromatic, and positively charged species. (thomassci.com)

The Octet ® BLI platform also provides robust monitoring of biomolecular interactions to overcome limitations in throughput, performance and cost associated with traditional technologies such as enzyme-linked immunosorbent assay (ELISA) or high-performance liquid chromatography (HPLC). (sartorius.com)

In HILIC mode, water acts as the strongest solvent to effectively elute analytes off the column, and the organic solvent acts as the weak solvent. (thermofisher.com)

A wide variety of matrix-specific sample preparation techniques are available to help isolate and concentrate analytes and sample matrices prior to GC analysis. (sigmaaldrich.com)

With a wide range of ligand capture surfaces that allow for immobilization of antibodies, recombinant proteins, DNA, RNA and virus-like particles, the Octet ® BLI platform has become a must-have laboratory tool for biomolecular interactions studies. (sartorius.com)

During exposure, analytes interact with the sensing material. (wikipedia.org)
Analysis of the array image prior to exposure to analyte reveals that there is little interspot variation within protein blocks but that different amounts were actually immobilized for each protein ( Figure 1, top panel ). (genengnews.com)
A study that uses (a) biomedical testing or (b) the measurement of a substance [an analyte ], its metabolite , or another marker of exposure in human body fluids or tissues to confirm human exposure to a hazardous substance [also see exposure investigation ]. (cdc.gov)

This can include the antigen or the analyte of interest, such as the endogenous analytes from the patients, or even the labelled analyte from the assay reagent. (aacc.org)

The sensing material and the analyte can interact by covalent bonding, hydrogen bonding, or molecular recognition. (wikipedia.org)
Less appreciated is the fact that SPRi systems provide real-time data, which enables the user to monitor the progress of binding without disturbing molecular interactions. (genengnews.com)
Molecular imprinting is a process in which functional and crosslinking monomers are co-polymerized in the presence of the target analyte, the imprint molecule. (intechopen.com)
From the results, propose molecular mechanisms involved in the interaction cell membrane models/analytes. (sbpmat.org.br)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
This course covers modern mass spectrometry with a particular focus on the analysis of low molecular weight analytes. (lu.se)
Mass spectrometry for small molecules includes an overview of mass spectrometric techniques that can be used for analysis of low molecular weight analytes in both the gas and liquid phase. (lu.se)

immobilized doxorubicin aptamer onto planar and nanoporous electrodes via gold-thiol interaction. (stanford.edu)
Without the target analyte, the aptamer is unfolded and there is limited electron transfer between reporter and electrode. (stanford.edu)

Selectivity differences arise from the interactions of the different counter ions forms with the hydroxyl groups on the analyte molecules. (thermofisher.com)
The nature of the molecules under analysis can also mean that surface interactions are potentially problematic. (technologynetworks.com)

If an analyte is loaded in a solvent that is too highly eluting, then analyte breakthrough may occur or final elution volumes may be larger than necessary. (chromatographyonline.com)
For hydrophobic sorbents (and analytes), use a sample diluent with as little organic solvent as possible to ensure sample solubility. (chromatographyonline.com)
Optimization of wash solvent strength should be performed by titrating the elution strength in discrete steps over several tubes or wells containing the same nominal analyte amount, plotting the solvent strength against analyte recovered, choosing the highest solvent strength that does not contain any analyte response. (chromatographyonline.com)
Remember here also that the organic solvent type and composition can be used in tandem with solution pH and ionic strength in order to achieve a "strong" wash solvent that removes a large amount of sample matrix components but does not cause analyte breakthrough. (chromatographyonline.com)

Near-infrared (NIR) spectroscopy measures the interaction of near-infrared light with a sample, including transmission, reflectance and absorbance, facilitating the identification of analytes. (selectscience.net)

The columns can also be run at elevated temperatures for faster analysis and improved resolution of some closely eluting analytes. (thermofisher.com)

End-capped to minimize polar interactions. (thomassci.com)
Recommended for strong non-polar analyte extractions. (thomassci.com)
Non-polar analytes should be more strongly retained compared with non-endcapped SampliQ C18 products. (thomassci.com)

Part 1: The group will design a biosensing assay with a recognition element that either is based on the interaction of the analyte with an enzyme, antibody, DNA/RNA or cells/tissue. (lu.se)

Response Surface Methodology was used to evaluate the interactions of carbonization temperatures of adsorbents and concentrations of adsorbates on the adsorption capacities and removal efficiencies of activated carbons obtained from flamboyant ( Delonix regia ) pod bark (FB), milk bush ( Thevetia peruviana ) kernel shell (MB) and rice ( Oryza sativa ) husks (RH). (omicsonline.org)

During this process, losses of the analyte can occur through interaction with the component material of the tubing or sampling container. (cdc.gov)

It was found that the presence of multiple negatively charged groups in the analyte were required to establish sufficiently strong interactions to permit binding at low micromolar concentrations in aqueous buffer. (europa.eu)
We used a series of multiplex assays to measure the concentrations of 55 serum analytes in specimens from patients from that outbreak to identify biomarkers specific to pediatric disease. (cdc.gov)
In this study, we used a series of multiplex assays to measure the concentrations of 55 serum analytes in specimens from patients from the Gulu outbreak to identify biomarkers that had age-specific associations with survival, hemorrhagic manifestations, or both. (cdc.gov)
Two factors interaction (2FI) model Y = a 0 + a 1 A + a 2 B + a 12 AB, showed that the interactions between increasing activation temperature of the adsorbents and increasing concentrations of the adsorbates increased the adsorption capacity of the MB while the adsorption capacities of FB and RH were reduced. (omicsonline.org)

Moreno-Castilla [ 12 ] reported that, there is complex interplay between electrostatic and non-electrostatic interactions while both are highly influenced by the surface chemistry of the carbon. (omicsonline.org)

Studies were performed to investigate the UDP-glucuronosyltransferase enzyme(s) responsible for the human liver microsomal N 2-glucuronidation of the anticonvulsant drug lamotrigine (LTG) and the mechanistic basis for the LTG-valproic acid (VPA) interaction in vivo. (aspetjournals.org)
Investigate the interactions between the analytes of interest and the cell membrane models using micro-Raman and SERS spectroscopy. (sbpmat.org.br)
The purpose of this study was to investigate this interaction between hydrogen halide gases and various types of common laboratory tubing. (cdc.gov)

This area includes novel sensing modalities and materials, sensing mechanisms, analyte-probe interactions and actuation/control methods. (anl.gov)

The sensing material has an inherent resistance that can be modulated by the presence or absence of the analyte. (wikipedia.org)

To achieve analytical success, it is necessary to develop thorough clean-up procedures to extract analytes from the matrix. (hindawi.com)

In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. (nature.com)
Thus, the potential exists for inhibitory drug-drug interactions. (aspetjournals.org)
Recent work in this and other laboratories has explored in vitro approaches for predicting the hepatic clearance of glucuronidated drugs in vivo and the magnitude of inhibitory drug interactions involving glucuronidated drugs. (aspetjournals.org)

Because light interacts with analytes in the tissue, PI is better when the design includes longer optical path length to allow greater interaction. (machinedesign.com)
PI is worse when air gap and/or cover glass that poorly matches the refractive index of tissue would also attenuate more LED light before it can interact with the analyte. (machinedesign.com)

The analyses cover a wide range of topics, from the investigation of the host-microbiome interaction to effects of chemicals on organ or cellular level. (ufz.de)

The analyte, which may likewise be any type of biological sample, is flowed over the entire array so that all probes are exposed to exactly the same solution and all measurements are collected simultaneously. (genengnews.com)
After sample collection and preparation, the analytes of interest are separated inside the column and then a detector measures the quantity of the components that exit the column. (sigmaaldrich.com)
Crude Sample Compatibility - Analytes can be measured in crude, unpurified mixtures such as cell lysates or hybridoma supernatants. (sartorius.com)
sample generic zoloft of the analyte has a vital role to other locations and laboratories. (drapikowski.pl)
If ion-exchange sorbents are used, the pH of the sample diluent should be adjusted to ensure target analytes are in their ionized form before loading. (chromatographyonline.com)
These interferences are caused when a sample contains elements or compounds that have analytical emission lines (bands) that overlap the line chosen for the analyte. (cdc.gov)
For example, if the analyte is mercury, the laboratory test will determine the amount of mercury in the sample. (cdc.gov)

The therapeutic efficacy of drugs is mediated by physical interaction with their cognate targets (mainly proteins). (nature.com)
non-canonical EGFR-Met interactions are implicated in resistance to anti-cancer drugs but have not been previously detected in drug-naïve cells. (nature.com)
The developed system is able to produce distinct responses (dual channel) upon interaction with hypoxia and NO. This probe can also release anticancer drugs upon photoirradiation acting potentially as both a dual-analyte imaging agent and a prodrug. (rsc.org)

Real-time Analysis - Binding interactions are continuously monitored as they happen. (sartorius.com)

To analyze protein function, the array was then exposed to three different analyte solutions sequentially ( Figure 1 ). (genengnews.com)
Each analyte bound specifically to its probe demonstrating protein functionality was preserved. (genengnews.com)
Replicate probes for each protein bound reproducible quantities of analyte ( Table ). (genengnews.com)
Although protein-protein interactions (PPIs) are promising targets, drugging them is one of the key challenges in drug discovery 19 . (nature.com)

He's also focused on building and reinforcing capacity for public health laboratory testing by fostering interactions between public and private laboratories. (cdc.gov)

Independent of these, its biophysical properties and peptide interactions point to still further roles of C-peptide, in particular regarding possible. (researchgate.net)

The entire complex becomes bound to the solid phase via interaction of biotin and streptavidin. (cdc.gov)

These interactions cause changes in the resistance reading. (wikipedia.org)

this allows for the amount of analyte present to be measured. (wikipedia.org)
By comparing the response characteristics with a calculated theoretical zero interaction, the amount of interaction can be estimated. (cdc.gov)

Chemiresistors are a class of chemical sensors that rely on the direct chemical interaction between the sensing material and the analyte. (wikipedia.org)

A positive outcome of the project is a deeper understanding of how multivalent interactions can be used to regulate (bio)chemical processes. (europa.eu)

Consistent with published data for the effect of fluconazole on zidovudine glucuronidation by human liver microsomal UGT2B7, the K i value generated in the presence of BSA predicted the magnitude of the LTG-VPA interaction reported in vivo. (aspetjournals.org)

Traditional hardware can lead to non-specific interactions, affecting analyte recovery and sensitivity. (selectscience.net)
For the avoidance of doubt: in solid-phase extraction, the ability to be selective invariably equates to the extent of analyte recovery. (chromatographyonline.com)

In this video we will take a more in depth look at these important interactions. (chromacademy.com)

Importantly, this discovery led to the unexpected insight that this interaction can be used to create supramolecular systems with a well-defined lifetime by adding enzymes able to cleave negatively charged groups from the substrate (which included phosphorylated sugars). (europa.eu)

1966). This technique is based on the interaction of the nuclei of individual barium atoms with neutron irradiation, resulting in the emission of x-rays (photons). (cdc.gov)

The rapid transit of furoxone the analyte or by direct UV. (eventenergy.ru)

In a PPG, the system generates a train of light pulses that are received at photodetector(s) after the light passes through the analyte. (machinedesign.com)
That's because tissues rich in capillaries would allow greater interaction between the light and the analyte, thus improving PI. (machinedesign.com)
The interaction between the excitation light and the analyte is enhanced by anti-resonantly guiding the excitation light within the analyte-containing fluid. (parc.com)

Ten analytes were significantly altered in cases compared to controls and were clustered into four principal components: PC1 (VEGF, MIP-1β, VEGF-C and IL-4), PC2 (MCP-1, IL-2 and IL-12p70), PC3 (VEGF-D) and PC4 (Eotaxin-3). (lu.se)

Adult patients had similar levels of these analytes regardless of outcome. (cdc.gov)

The concept of DCC on gold nanoparticles was validated using metal ions as analytes, rather than carbohydrates, because of the higher strength of interaction with the recognition modules. (europa.eu)
This equivalent "X-material shield assembly" (Patent Pending) enables the harder PuBe source spectrum (average energy of 4.61 MeV) from a small encapsulated standard 1-Ci PuBe source to be transformed, through interactions in the shield, so that leakage neutrons are shifted in energy and yield to become a close reproduction of the neutron spectrum leaking from a large subcritical mass of WGPu metal (mean energy 2.11 MeV). (spie.org)

Using a bianalyte strategy in which dithiopurine (DTP) and ethanol were employed as the model analytes, we demonstrated that AuNPs enhance DTP's Raman signal but attenuate ethanol's Raman intensity. (acs.org)

Anatomy9

Organisms1

Diseases1

Chemicals and Drugs118

Analytical, Diagnostic and Therapeutic Techniques and Equipment83

Phenomena and Processes16

Disciplines and Occupations14

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture20

Named Groups1

Health Care20

Detection8

Sorbent11

Stationary phase4

Retention5

Concentration3

Aromatic1

Chromatography1

Elute1

Matrices1

Biomolecular interactions1

Exposure3

Antigen1

Molecular7

Aptamer2

Molecules2

Solvent4

Absorbance1

Columns1

Polar3

Antibody1

Methodology1

Occur1

Concentrations4

Surface1

Investigate3

Mechanisms1

Absence1

Extract1

Drug interactions3

Tissue2

Investigation1

Sample7

Drugs3

Analysis1

Protein4

Laboratory1

Biophysical1

Bound1

Resistance1

Amount2

Sensors1

Processes1

Presence1

Recovery2

Important1

Groups1

Technique1

Direct1

Light3

Components1

Patients1

Metal2

Model1