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Chromatography, AffinityGelsChromatography, High Pressure LiquidChromatography, GelChromatography, Ion ExchangeChromatographySepharoseMolecular WeightMolecular Sequence DataElectrophoresis, Polyacrylamide GelAmino Acid SequenceKineticsChromatography, GasProtein BindingChromatography, LiquidChromatography, Thin LayerBinding SitesChromatography, DEAE-CelluloseSubstrate SpecificityRecombinant ProteinsAffinity LabelsChromatography, AgaroseLectinsEscherichia coliAmino AcidsBase SequenceHydrogen-Ion ConcentrationBinding, CompetitiveIsoelectric FocusingCattleAntibody AffinityMass SpectrometryRabbitsCloning, MolecularElectrophoresis, Gel, Two-DimensionalPeptide FragmentsCarrier ProteinsCarbohydratesMacromolecular SubstancesPlant LectinsCell LineChemistryChemical PhenomenaGlycoproteinsLigandsSequence Homology, Amino AcidRecombinant Fusion ProteinsImmunodiffusionTrypsinPeptidesIsoelectric PointBacterial ProteinsCell MembraneLiverSolubilityCarbohydrate SequenceOligosaccharidesTemperatureProtein ConformationStructure-Activity RelationshipPolysaccharidesAntibodies, MonoclonalDNASwineHeparinGas Chromatography-Mass SpectrometryHemagglutinationHydrolysisModels, MolecularAntibodiesChemical FractionationBlotting, WesternReceptors, Cell SurfaceIsoenzymesProtein Structure, TertiaryElectrophoresis, Agar GelMembrane ProteinsSpectrophotometry, UltravioletGlycopeptidesTandem Mass SpectrometryImmune SeraCells, CulturedGlycosylationDetergentsMutationMethodsChromatography, PaperSpecies SpecificityProteinsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationChromatography, Reverse-PhaseImmunoelectrophoresisSurface Plasmon ResonanceRats, Inbred StrainsDrug StabilityAntibody SpecificityGalectinsCarbohydrate ConformationCross-Linking ReagentsMutagenesis, Site-Directed
PurificationResinsFiltration chromatographyProteinsResinCationIMACHplcElectrophoresisBiomoleculesSeparationAntibodyLiquidColumnsDownstreamRecombinantAgaroseMoleculesAssayBufferMembraneLigandLectinChromatin immunoprecipitationCompoundsExchangePeptidesMoleculeMechanismSpecificProtein InteractionsAminoTechniquesEnzymeInsightsMethodsMini gelOrganicSodiumStudyRabbitAnalysisAtomicProductWeakSerum
Purification13
  • Explore a complete line of chromatography resins and media with various base bead and ligand chemistries for your lab-scale and bioproduction purification needs. (bio-rad.com)
  • From high-throughput resin screening to small-scale method development and scale-up optimization, find chromatography resins in prepacked formats for the entire purification development cycle. (bio-rad.com)
  • dc.title: Evaluating Improvements and Challenges in Affinity Chromatography for AAV Purification dc.description.abstract: Purification process development is crucial to the Research and Development (R&D) sector in the biotechnology industry by designing and optimizing downstream unit operations capable of manufacturing safe treatments for clinical trials. (jhu.edu)
  • An assortment of affinity purification resins for the separation of both tagged and immunoglobulin molecules. (gbiosciences.com)
  • Calmodulin resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells are available as resin only or in kit form including binding and elution buffers. (gbiosciences.com)
  • Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. (gbiosciences.com)
  • Liquid chromatography (LC) is a common purification technique with liquid mobile phases. (amerigoscientific.com)
  • The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification. (gbiosciences.com)
  • In the downstream separation and purification process of biopharmaceuticals, chromatography is one of the indispensable separation methods. (biolink.com)
  • Hydrophobic chromatography is a chromatographic mode for purification based on the difference in hydrophobicity between the target product and impurities. (biolink.com)
  • The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
  • The latest manual in the Basic Methods series contains a collection of convenient and easy to use protein purification protocols along with a sampling of dependable methods for assessing protein-protein interactions. (cshlpress.com)
  • The collection of essential methods found in Basic Methods in Protein Purification and Analysis is mainly drawn from the popular manuals Proteins and Proteomics, Purifying Proteins for Proteomics, and Protein-Protein Interactions, 2nd Ed. In addition to protocols for purification using gel electrophoresis and column chromatography, this book contains tested methods for preparing cellular and subcellular extracts-a critical and often neglected step in successful protein purification. (cshlpress.com)
Resins8
  • Purify affinity-tagged proteins, monoclonal antibodies, and other biomolecules with a range of affinity resins including immobilized metal affinity chromatography (IMAC), Protein A, and activated resins. (bio-rad.com)
  • Purify charged and polar molecules with anion and cation exchange chromatography (AEX, CEX) resins available in various particle sizes, ionic forms, and binding capacities. (bio-rad.com)
  • Experience unparalleled selectivity, resolution, yield and ease-of-use for a variety of biomolecules with the unique separation properties of our multimodal chromatography resins. (bio-rad.com)
  • Explore a wide selection of size exclusion (or gel filtration) chromatography resins for diverse applications, including those requiring use of organic eluents. (bio-rad.com)
  • High affinity biotin tagged protein resins are available along with streptavidin binding and elution buffers and reagents for the quantitation of biotin . (gbiosciences.com)
  • Our packings and media mainly consist of silica gels or polymeric resins for normal phase, reversed phase, affinity, ion exchange, and hydrophilic interaction chromatography. (amerigoscientific.com)
  • On the basis of product, the market is classified into chromatography columns and resins, filters, membrane adsorbers, single-use products, and other products. (marketsandmarkets.com)
  • In 2016, the chromatography columns and resins segment accounted for the largest share of the market. (marketsandmarkets.com)
Filtration chromatography2
Proteins9
  • For this reason, this study aims to clone, express, purify, and characterize Dengue ns5 and its interactions with other Dengue proteins. (umsystem.edu)
  • FPLC (Fast Protein Liquid Chromatography) is, as its name suggests, a liquid chromatography that is often used to analyze or separate compounds from a mixture of proteins. (quimigen.com)
  • Based upon its specific interactions with both cells and extracellular matrix components, thrombospondin has been hypothesized to be a member of a class of extracellular proteins which may modulate cell-matrix interactions (24). (goprolytix.com)
  • The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. (biomedcentral.com)
  • When an ion exchange chromatography column is loaded with a sample at a particular pH, all proteins that are appropriately charged will bind to the resin. (blueprintprep.com)
  • Even very similar components, such as proteins that may only vary by a single amino acid, can be separated with chromatography. (blueprintprep.com)
  • Based on this, an affinity chromatography for recombinant tagged proteins was developed. (tum.de)
  • Rounding out the manual are methods for characterizing protein-protein interactions, an extensive appendix of essential methods for quantifying protein concentration, stabilizing and storing proteins, concentrating proteins, and immunoblotting. (cshlpress.com)
  • Total cell lysates (15 µg total protein) from A431, SH-SY5Y, MCF-7 (reduced expression control), and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL (1:1000 dilution) Purified anti-EG5 Antibody, clone 10C7, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. (biolegend.com)
Resin4
  • Column resin is the most common affinity chromatography matrix, but it possesses mass transfer limitations. (jhu.edu)
  • This report evaluated a prototype affinity membrane device coupled with AVB ligand designed to overcome these mass transfer limitations and decrease process times, while yielding comparable AAV recovery and purity to column resin. (jhu.edu)
  • Compounds can be separated by their size (size exclusion chromatography (SEC)), by their charge distribution (ion exchange chromatography) or by their biological or chemical affinity for the resin used (affinity chromatography). (quimigen.com)
  • How To Select The Appropriate Chromatography Resin For My Own Process? (biolink.com)
Cation1
  • The ns5 was purified to homogeneity by successive chromatographic separations: immobilized metal affinity chromatography, FPLC, cation exchange and FPLC gel filtration. (umsystem.edu)
IMAC1
  • Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1975), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. (gbiosciences.com)
Hplc2
  • High performance liquid chromatography (HPLC) columns use higher pressures and smaller solid particles, which not only overcome some limitations of standard LC, but also allow for more effective separations and wider applications, such as pharmaceutical factory, environment, forensics, and chemical industry. (amerigoscientific.com)
  • The presence of HMW species, for instance, is frequently determined using size-exclusion chromatography (SEC) coupled with high-pressure liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC), while LMW species are detected using a capillary electrophoresis-sodium dodecyl sulfate (CE-SDS) (reduced and non-reduced) technique. (pharmtech.com)
Electrophoresis4
Biomolecules2
  • This method separates biomolecules based on a specific binding interaction between a ligand and a binding partner. (blueprintprep.com)
  • Silica gel is the most widely used base material in liquid chromatography, although the interactions of biomolecules at the aqueous interface are not fully understood. (tum.de)
Separation1
Antibody3
  • Milk, Casein and Blotto might interfere with antibody - antigen interaction. (enzolifesciences.com)
  • The antibody was purified by affinity chromatography. (biolegend.com)
  • Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. (biolegend.com)
Liquid1
  • 1984. Application of high-performance liquid chromatography with synchronized accumulating radioisotope detector to analysis of glyceryl trinitrate and its metabolites in rat plasma. (cdc.gov)
Columns2
  • For ease of use, a wide range of pre-filled columns for techniques such as ion exchange, gel filtration (size exclusion), hydrophobic interaction and affinity chromatography are available. (quimigen.com)
  • In addition, Bio-Link also produces chromatography columns and prepacked columns from laboratory to industrial grade. (biolink.com)
Downstream1
  • One of the key polishing steps in the downstream manufacturing process of AAVs is affinity chromatography, where a ligand with specific binding affinity to an AAV serotype is coupled to a base matrix that separates AAVs from host cell contaminants produced in the cell culture process. (jhu.edu)
Recombinant1
  • Using recombinant variants of ppFhCL3 we demonstrated the critical importance of a pair of propeptide residues (Tyr 46 Lys 47 ) for the interaction with the propeptide binding loop (PBL) of the mature enzyme and other residues (Leu 66 and Glu 68 ) that allow the propeptide to block the active site. (biomedcentral.com)
Agarose3
  • Agarose bound Sambucus nigra lectin is prepared using our affinity-purified lectins. (vectorlabs.com)
  • 1) Draw (pipet) the desired amount of settled agarose-lectin (gel) from the stock bottle into the prepared column and let the buffer drain by gravity. (vectorlabs.com)
  • In this study, HSPGs extracted from embryonic rat spinal cord by detergent were purified by ionexchange chromatography, gel filtration, and affinity chromatography on an agarose column coupled with FN‐C/H II conjugated to ovalbumin (OA). (umn.edu)
Molecules7
  • A large selection of affinity coupling chemistries are available to isolate your target molecules including carboxyl containing molecules, nucleic acids, glycoproteins, polyclonal antibodies, oxidized sugars and many more. (gbiosciences.com)
  • Chromatography encompasses a wide range of methods used to separate and/or analyze complex mixes of chemical and biochemical molecules. (blueprintprep.com)
  • An important part of affinity chromatography design is choosing the proper binding material, so be on the lookout for any clues the tests gives us as to the interactions used by the target molecules. (blueprintprep.com)
  • A mixture (including the target molecules) is filtered through a gel, inside of which are spherical beads containing pores of a specific size. (blueprintprep.com)
  • Molecules of different sizes are included or excluded from the pores within the gel. (blueprintprep.com)
  • In medicine, chromatography is advantageous since it can separate even fragile molecules. (blueprintprep.com)
  • A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
Assay2
  • Various techniques can be used to detect the protein-peptide interaction including classic protease assay, gel filtrations and affinity chromatography. (kennesaw.edu)
  • This lectin has the capacity to interact with actin, since it binds to actin-F in a cosedimentation assay and it acts as a mediator in the binding of actin to the affinity column. (illinois.edu)
Buffer3
  • The oligosaccharides generated up to degree of polymerization 16 were characterized by high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) and electrospray ionization mass spectrometry (ESI-MS). Acetate buffer linear gradients were used as mobile phases for separations of oligosaccharides. (maigrir-regime-musculation.com)
  • Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. (vectorlabs.com)
  • 2) Wash the gel with 10 column volumes of buffer, such as HBS (10 mM HEPES, 0.15 M NaCl, pH 7.5) and discard the flow through. (vectorlabs.com)
Membrane3
  • Orai1 is a plasma membrane protein that in its tetrameric form is responsible for calcium influx from the extracellular environment into the cytosol in response to interaction with the Ca(2+)-depletion sensor STIM1. (rcsb.org)
  • Chemical cross-linking provides detailed insights into these interactions suggesting a role for membrane fusion. (nature.com)
  • Zippering of the SNAREs is proposed to provide the required energy to initiate membrane fusion 6 , 7 and proceeds from the N-terminus towards the membrane-proximal C-terminus through interactions of their complementary SNARE motifs. (nature.com)
Ligand1
  • The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)
Lectin1
  • Haemagglutination inhibition assays of different saccharides, glycoproteins and glycolipids indicate that this lectin has affinity for sialic acid, which is enhanced by its O-acetylation. (illinois.edu)
Chromatin immunoprecipitation1
  • While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. (fishersci.com)
Compounds1
Exchange1
Peptides2
  • Increasing the temperature from 298K-313K, increased the value of Ksv and pointed to a dynamic quenching mechanism for Aβ peptides, nps and curcumin interaction with nNOS. (ru.ac.za)
  • The positive signs for entropy and enthalpy for all Aβ peptides nps and curcumin pointed to hydrophobic-hydrophobic interaction with the enzyme. (ru.ac.za)
Molecule1
  • Suitable for kinase molecule interaction analyses using SPR , as substrate for kinase activity assays and for western blot analyses. (proteinkinase.biz)
Mechanism1
  • In this study, the binding affinity and mechanism of human odor-binding protein OBP2a to 14 typical odorants in water were first assessed using fluorescent competitive binding assays and molecular docking techniques. (springeropen.com)
Specific4
  • These values are at least 1000-fold lower than those K i obtained for human cathepsin L (HsCL) and human cathepsin K (HsCK) demonstrating the selectivity of the ppFhCL3 for parasite cathepsins L. By exploiting 3-D structural data we identified key molecular interactions in the specific binding between the ppFhCL3 and FhCL3 mature domain. (biomedcentral.com)
  • Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
  • As a result a specific signal is produced, indicating an interaction has taken place. (genomicglossaries.com)
  • It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. (thermofisher.com)
Protein Interactions2
  • In this study, we monitor the peptide-protein interactions without immobilized or chemical derivatization. (kennesaw.edu)
  • co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)
Amino1
  • We solved the X-ray crystal structure of six-helical bundle (6-HB) core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit, revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain. (nature.com)
Techniques1
  • While the MCAT will not expect us to be chromatography experts, the test makers expect us to know the fundamentals, how/when to apply appropriate techniques, and will expect us to make conclusions based on reported results. (blueprintprep.com)
Enzyme1
  • Thus, the interaction of Aβ with nNOS could serve to regulate the interaction between nNOS and arginine by restoring activity of the enzyme but at the same time promoting fibrillogenesis. (ru.ac.za)
Insights2
Methods1
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
Mini gel1
  • Western Blot (20µl/lane (mini gel) for HRPO/DAB detection, or 5µl/lane (mini gel) for HRPO/ECL detection. (enzolifesciences.com)
Organic1
  • Trace metals and metal-organic interactions in natural waters. (cdc.gov)
Sodium1
  • After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline, containing 0.08% sodium azide for storage. (vectorlabs.com)
Study1
  • The host-pathogen interactions that define a disease are clearly complex, and, in many cases, the study of these interactions is limited by the lack of a suitable animal model. (cdc.gov)
Rabbit1
Analysis3
Atomic1
  • The interaction between Orai1 and CaM at the atomic level remains unknown. (rcsb.org)
Product3
Weak1
  • Physisorption occurs due to weak van de Waals interactions that exist between an adsorbent and its target material, for instance, London dispersion, dipole interactions, or hydrogen bonds. (ablogwithadifference.com)
Serum2
  • Serum collected using standard sampling tubes or tubes containing separating gel. (cdc.gov)
  • Stability of serum obtained with tubes containing separating gel: 48 hours at 2-8°C (note the data provided by the tube manufacturer). (cdc.gov)