CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. (thermofisher.com)
Activity Assay: A representative lot of the original monoclonal antibody has been shown to convert alpha5 beta1 integrin into a ligand competant form and promotes fibronectin binding. (ximbio.com)
Detects human Integrin alpha 5/CD49e in Western blots. (rndsystems.com)
The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). (rndsystems.com)
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The 5H10-27 (MFR5) monoclonal antibody specifically binds to the α5 chain of the integrin α5β1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, precursor and activated B cells, mast cells, platelets, epithelial and endothelial cells, fibroblasts and a variety of mouse cell lines. (bdbiosciences.com)
The following product was used in this experiment: CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Biotin, eBioscience™ from Thermo Fisher Scientific, catalog # 13-0519-82, RRID AB_529536. (thermofisher.com)
Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. (thermofisher.com)
Fibronectin3
Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1. (rndsystems.com)
Schultz JF, Armant DR. Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. (bdbiosciences.com)
In this regard, the βI domain-dependent clustering of active β1 integrins is necessary to favor fibronectin-elicited directional EC motility, which cannot be effectively promoted by β1 integrin conformational activation alone. (life-science-alliance.org)
The aim of this study was to examine the role of integrin subunits and its down-stream signaling molecules in the cyclic hydrodynamic pressure-induced proliferation of human bladder smooth muscle cells (HBSMCs) cultured in scaffolds. (bvsalud.org)
Real-time RT-PCR and western blotting were used to examine the expression levels of integrin subunits and their downstream signaling molecules. (bvsalud.org)
The researchers located that the immune cells which had high levels of alpha-5 integrin likewise produced more inflammatory molecules. (paramountcontractingservices.com)
Moreover, inhibition of integrin alpha5 decreased the level of p-FAK and abolished proliferation of HBSMCs stimulated by cyclic hydrodynamic pressure. (bvsalud.org)
Cytoplasmic2
The N-terminus of Nischarin interacts with a short segment of the cytoplasmic domain of the alpha5 integrin subunit. (nih.gov)
Furthermore, recombinant calreticulin reacted in surface plasmon resonance analysis (SPR) with peptides corresponding to cytoplasmic signalling domains of the integrins alphaIIb and alpha5, in a calcium independent manner. (cardiff.ac.uk)
Reactivity1
In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 is observed and less than 2% cross-reactivity with recombinant human Integrin alpha M, rmIntegrin alpha 2, rmIntegrin alpha 4, and rmIntegrin alpha E is observed. (rndsystems.com)
Receptor1
Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes. (bdbiosciences.com)
Pathway3
Thus integrins modulate the efficiency of the Erk (extracellular-signal-regulated kinase)/MAP kinase (mitogen-activated protein kinase) pathway, acting at several distinct levels. (nih.gov)
We have shown that both cAMP-dependent protein kinase and PAKs (p21-activated kinases) play a role in integrin regulation of the Erk pathway, acting primarily at the level of Raf-1. (nih.gov)
Taken together, we demonstrate for the ?rst time that the integrin alpha5-FAK signaling pathway controls the proliferation of HBSMCs in response to cyclic hydrodynamic pressure. (bvsalud.org)
Antibody1
Treatment with an anti-alpha-5/beta-1 integrin antibody delayed disease progression in mice and extended survival. (alsnewstoday.com)
Receptors1
Gomez M, Cano A. Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells. (bdbiosciences.com)
Adhesion2
In addition to adhesion, integrins are known to participate in cell surface mediated signalling. (ximbio.com)
Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. (nih.gov)
Promotes2
In addition, SPON2 promotes cytoskeletal remodeling and transendothelial migration of monocytes by activating integrin β1/PYK2 axis. (biomedcentral.com)
By exploiting four monoclonal antibodies recognizing distinct conformational epitopes, we show that in endothelial cells (ECs), the extracellular βI domain, but not the hybrid or I-EGF2 domain of active β1 integrins, promotes their FAK-regulated clustering into tensin 1-containing fibrillar adhesions and impairs their endocytosis. (life-science-alliance.org)
Expression1
Appears to play a role in wound healing by up-regulating, in skinfibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. (nih.gov)
Protein2
Thus we have discovered a novel protein that links the alpha5beta1 integrin to migration controlled by Rho-family GTPases. (nih.gov)
The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. (nih.gov)
Immune cells2
In this study, scientists found alpha-5/beta-1 integrin levels were markedly high in microgila and macrophages, immune cells that work to engulf and destroy threats and are known to play a role in the progression of ALS . (alsnewstoday.com)
In patients, immune cells positive for the integrin were located close to motor neurons and in regions where motor neurons were previously found. (alsnewstoday.com)
Regulation1
The dynamic regulation of the physical interactions of integrins with the ECM is necessary for physiological function, and it is disrupted in pathological settings ( Seguin et al, 2015 ). (life-science-alliance.org)
Role7
Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. (bdbiosciences.com)
Integrins clearly play a key role in regulating both mitogenic signalling and cell migration. (nih.gov)
Integrins and PAKs also play a role in the control of cell migration. (nih.gov)
Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. (ximbio.com)
The findings highlighted the role of the alpha-5/beta-1 integrin "on different cell types involved in neuroinflammation in ALS and has shown that blocking α5β1 integrin is a therapeutic target for the treatment of ALS," Marques said. (alsnewstoday.com)
According to previous studies, integrins play an important role in the mechanotransduction. (bvsalud.org)
Staining of M21 cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.5 µg of Anti-Human CD51/CD61 (Integrin alpha/beta 3) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). (thermofisher.com)
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. (ximbio.com)
In a collaborative effort between Pasithea and researchers at Stanford, the Mayo Clinic, and Oregon Health & Science University, the alpha-5/beta-1 integrin was identified as a possible target for easing nerve cell loss in ALS. (alsnewstoday.com)
I have spent my career studying integrins and I believe [alpha-5/beta-1] integrin is an important therapeutic target for the treatment of ALS," said Lawrence Steinman, a neurologist and professor at Stanford Medical School, and co-lead author of the breakthrough study. (alsnewstoday.com)
Surface1
Integrins are found on the surface of cells and are associated with assisting cells connect to neighboring cells. (paramountcontractingservices.com)
Studies1
These studies emphasize the intricate connection between integrins and Rho-family GTPases and their effectors in controlling both mitogenesis and migration. (nih.gov)
Function1
Modulation of integrin function is required in many physiological and pathological settings, such as angiogenesis and cancer. (life-science-alliance.org)