• Mouse splenocytes were stained with (A) Rat Anti-Mouse Integrin a2/CD49b Monoclonal Antibody (Catalog # MAB1740) or (B) Rat IgG2B isotype control antibody ( MAB006 ), followed by APC-conjugated Anti-Rat IgG Secondary Antibody ( F0113 ) and Rat anti-Mouse NKp46 PE-conjugated Monoclonal Antibody ( FAB22252P ). (rndsystems.com)
  • The following product was used in this experiment: CD51/CD61 (Integrin alpha v beta 3) Monoclonal Antibody (23C6), Biotin, eBioscience™ from Thermo Fisher Scientific, catalog # 13-0519-82, RRID AB_529536. (thermofisher.com)
  • Description: The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin alphav/beta3. (thermofisher.com)
  • Antibody inhibitors and siRNA were used to manipulate the expression profiles of LX-2 cells that were incubated under normal culture conditions or after treatment with TGF-Β, a known ligand for the αv integrins and a pro-fibrotic cytokine, to mimic a pro-fibrotic environment in vitro. (harvard.edu)
  • The integrin profile of LX-2 cells was not changed by antibody treatment targeting αvβ3 or αvβ5. (harvard.edu)
  • Here, we highlight recent insights into integrin control of intracellular trafficking in processes such as degranulation, mechanotransduction, cell-cell communication, antibody production, virus entry, Toll-like receptor signaling, autophagy, and phagocytosis, as well as the release and uptake of extracellular vesicles. (uu.nl)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Integrin Alpha 4 (ITGa4) in Tissue homogenates, cell lysates and other biological fluids. (ibiomagazine.org)
  • Scholars@Duke publication: Randomised trial of coronary intervention with antibody against platelet IIb/IIIa integrin for reduction of clinical restenosis: results at six months. (duke.edu)
  • We tested a monoclonal antibody Fab fragment (c7E3) directed against the platelet glycoprotein IIb/IIIa integrin, the receptor mediating the final common pathway of platelet aggregation, to see whether it reduced the frequency of clinical restenosis. (duke.edu)
  • Possible Efficacy of Vedolizumab, an Anti-α4ß7 Integrin Antibody, in Palmoplantar Pustulosis. (bvsalud.org)
  • We report a patient with PPP improved by vedolizumab (anti-α4ß7 integrin antibody) treatment for ulcerative colitis , suggesting the possibility of a new molecular target for PPP therapy . (bvsalud.org)
  • Recognizes the α4 subunit of α4β1 integrin (also known as very late antigen 4 [VLA-4] or CD49d-CD29) and α4β7 integrin. (enzolifesciences.com)
  • Integrin alpha1beta1 functions as a receptor for LAMININ and COLLAGEN . (online-medical-dictionary.org)
  • providing a ligand for the α6β1 integrin receptor expressed by OLs. (jneurosci.org)
  • The coxsackievirus-adenovirus receptor (CAR) appears to play a major role during infection of most human serotypes, whereas the alpha (v)beta (3/5) integrins have been reported to play a significant auxiliary role. (cnrs.fr)
  • Expression of the cellular transmembrane receptor αvβ6 integrin is essentially restricted to malignant epithelial cells in carcinomas of a broad variety of lineages, while it is virtually absent in normal adult tissues. (cipsm.de)
  • Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
  • Integrin α-l/β-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. (lu.se)
  • Integrin α-m/β-2 is also a receptor for Factor X. Integrin α-d/β 2 is a receptor for ICAM3 and VCAM1. (lu.se)
  • Integrins are obligate heterodimers composed of α and β subunits. (wikipedia.org)
  • Several genes code for multiple isoforms of these subunits, which gives rise to an array of unique integrins with varied activity. (wikipedia.org)
  • In mammals, integrins are assembled from eighteen α and eight β subunits, in Drosophila five α and two β subunits, and in Caenorhabditis nematodes two α subunits and one β subunit. (wikipedia.org)
  • Through different combinations of the α and β subunits, 24 unique mammalian integrins are generated, excluding splice- and glycosylation variants. (wikipedia.org)
  • Integrin subunits span the cell membrane and have short cytoplasmic domains of 40-70 amino acids. (wikipedia.org)
  • The molecular mass of the integrin subunits can vary from 90 kDa to 160 kDa. (wikipedia.org)
  • The cations in the β subunits are more interesting: they are directly involved in coordinating at least some of the ligands that integrins bind. (wikipedia.org)
  • The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with the Integrin alpha 1 (CD29) or alpha 4 (CD104) subunits and binds Laminin. (rndsystems.com)
  • Integrin alpha 2 is one of twelve integrin family alpha subunits that share the beta 1 subunit (1‑3). (novusbio.com)
  • Integrin alpha 2 beta 1 is the non-covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits and is one of six very late antigens on activated T cells, designated VLA2 (3). (novusbio.com)
  • CD51, an ~120 kDa surface molecule can also non-covalently associate with other beta subunits of the integrin family including beta1 (CD29), beta5 and beta6 to form receptors for extracellular matrix components. (thermofisher.com)
  • There are 24 known integrin molecules classified into different families depending on the subunits (i.e. αv family of integrins) or by the ligands they bind to (i.e. (harvard.edu)
  • Thus integrins modulate the efficiency of the Erk (extracellular-signal-regulated kinase)/MAP kinase (mitogen-activated protein kinase) pathway, acting at several distinct levels. (nih.gov)
  • We have shown that both cAMP-dependent protein kinase and PAKs (p21-activated kinases) play a role in integrin regulation of the Erk pathway, acting primarily at the level of Raf-1. (nih.gov)
  • Integrin-linked kinase (ILK) is a major structural adaptor protein governing signaling complex formation and cytoskeletal dynamics. (jneurosci.org)
  • Integrin-linked kinase (ILK) is the binding partner of β1 and β3 integrins, and has been ascribed essential roles in development, angiogenesis and tumourigenesis. (bmj.com)
  • Testosterone levels315, an integrin-linked kinase (ILK) inhibitor, provides been proven to suppress the growth of breasts cancers, abdomen chronic and tumor lymphocytic leukemia cells. (exposed-skin-care.net)
  • Body 2 Testosterone levels315 induce dephosphorylation of proteins kinase T (Akt) without transformation of integrin-linked kinase (ILK) in AML cell lines. (exposed-skin-care.net)
  • Phosphatidylinositol 3-kinaseβ (PI3Kβ) plays a predominant role in integrin outside-in signaling and in platelet activation by GPVI engagement. (edu.au)
  • Find Integrin beta 2 ELISA Kits for a variety of species such as anti-Human Integrin beta 2, anti-Cow Integrin beta 2, anti-Mouse Integrin beta 2. (antibodies-online.com)
  • Detects mouse Integrin alpha 2/CD49b in direct ELISAs. (rndsystems.com)
  • In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed. (rndsystems.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Integrin Alpha 4 (ITGa4) in samples from tissue homogenates, cell lysates or other biological fluids. (ibiomagazine.org)
  • Integrins work alongside other proteins such as cadherins, the immunoglobulin superfamily cell adhesion molecules, selectins and syndecans, to mediate cell-cell and cell-matrix interaction. (wikipedia.org)
  • An acidic amino acid features in the integrin-interaction site of many ECM proteins, for example as part of the amino acid sequence Arginine-Glycine-Aspartic acid ("RGD" in the one-letter amino acid code). (wikipedia.org)
  • Despite many years of effort, discovering the high-resolution structure of integrins proved to be challenging, as membrane proteins are classically difficult to purify, and as integrins are large, complex and highly glycosylated with many sugar 'trees' attached to them. (wikipedia.org)
  • SHARPIN is now shown to negatively regulate integrin activation by binding the α-integrin subunit and interfering with the association of the β cytodomain with activating proteins. (bris.ac.uk)
  • Integrins are cell adhesion molecules that reside on the surface of cells as transmembrane proteins made of an alpha and beta subunit. (harvard.edu)
  • Whereas collagens are the major building blocks in tissues and defects in these structural proteins have severe consequences for tissue integrity, the mild phenotypes of the integrin type of collagen receptors have raised questions about their importance in tissue biology and pathology. (uib.no)
  • Second, the CAV-2 penton, hexon, and fiber proteins do not contain a known integrin-interacting motif. (cnrs.fr)
  • The peptide ligands containing the arginine-glycine-aspartic acid (RGD), which displays a strong binding affinity and selectivity to integrins, particularly to integrin αvβ3, have been developed to conjugate with various conventional chemotherapeutic agents, such as small molecules, peptides and proteins, and nanoparticle-carried drugs for integtrin targeted therapeutic studies. (thno.org)
  • Integrins are transmembrane receptors that help cell-cell and cell-extracellular matrix (ECM) adhesion. (wikipedia.org)
  • Upon ligand binding, integrins activate signal transduction pathways that mediate cellular signals such as regulation of the cell cycle, organization of the intracellular cytoskeleton, and movement of new receptors to the cell membrane. (wikipedia.org)
  • Integrins are found in all animals while integrin-like receptors are found in plant cells. (wikipedia.org)
  • Integrins are transmembrane cell surface receptors that mediate cell-cell and cell-matrix contacts. (bmj.com)
  • Integrins are transmembrane receptors that transduce biochemical and mechanical signals across the plasma membrane and promote cell adhesion and migration. (uu.nl)
  • As cell surface receptors, integrins readily interact with extracellular ligands and play a vital role in angiogenesis, leukocytes function and tumor development, which sets up integrins as an excellent target for chemotherapy treatment. (thno.org)
  • Integrins α-m/β-2 and α-x/β-2 are receptors for the IC3B fragment of the third complement component and for fibrinogen. (lu.se)
  • Ligands for integrins include fibronectin, vitronectin, collagen and laminin. (wikipedia.org)
  • Coating recombinant Alix on the culture substratum for NIH/3T3 cells promotes alpha5beta1-integrin-mediated cell adhesions and fibronectin assembly, and these effects require the aa 605-709 region of Alix. (nih.gov)
  • These findings demonstrate that a sub-population of Alix localizes extracellularly and regulates integrin-mediated cell adhesions and fibronectin matrix assembly. (nih.gov)
  • The objectives of this study were to characterize how the αv integrins regulate each other in both a normal and pro-fibrotic environment in order or decipher any mutual regulation between the αv integrins and to assess if there is a good in vitro model to look at potential compensation mechanisms specifically in an inflammatory environment. (harvard.edu)
  • OPN through interaction with CD44 or several integrins is known to regulate multiple macrophage functions. (cdc.gov)
  • Type 1 results from mutations in the integrin beta-2 gene ( ITGB2 ), encoding CD18 beta-2 integrins. (msdmanuals.com)
  • The N-terminus of Nischarin interacts with a short segment of the cytoplasmic domain of the alpha5 integrin subunit. (nih.gov)
  • Cytoplasmic face-mediated integrin inside-out activation remains a paradigm in transmembrane signal transduction. (rcsb.org)
  • We show here that, whereas membrane-proximal helices of integrin alpha/beta cytoplasmic tails associate in cytoplasm-like aqueous medium, they become partially embedded into membrane-mimetic micelles when unclasped. (rcsb.org)
  • Talin, a key intracellular integrin activator, recognizes this site as well as the membrane-proximal helix, thereby promoting cytoplasmic tail separation along the membrane surface. (rcsb.org)
  • These data provide a structural basis of membrane-mediated changes at the cytoplasmic face in regulating integrin activation and signaling. (rcsb.org)
  • The activity state of integrins is crucial for cell adhesion, migration and differentiation, and is regulated predominantly by protein interactions of the integrin β cytoplasmic domain. (bris.ac.uk)
  • Thus we have discovered a novel protein that links the alpha5beta1 integrin to migration controlled by Rho-family GTPases. (nih.gov)
  • Immortalized NIH/3T3 fibroblasts deposit less Alix on the substratum and have defects in alpha5beta1-integrin functions. (nih.gov)
  • We show here that the interactions between DV E protein, the sole mannosylated glycoprotein present on DV particles, and the C-type lectin dendritic cell-specific intercellular adhesion molecule 3-grabbing non-integrin (DC-SIGN) are essential for DV infection of DCs. (unboundmedicine.com)
  • Integrin alpha 3, also known as CD49c and VLA-3 alpha, is a type I membrane protein that heterodimerizes with Integrin beta 1. (rndsystems.com)
  • In addition to collagen-binding integrins, a group of proteinswith affinity both for fibrillar collagens and integrins link these two protein families together.We have introduced the name COLINBRI (COLlagen INtegrin BRIdging) for this set of molecules. (uib.no)
  • After 5 days, Progesterone and estradiol levels and endometrial tissues were investigated to evaluate of α4, αv, β1 and β3 integrins gene and protein by qPCR method and immunohistochemistry, respectively.Results: Tissue samples were assessed and showed that level of progesterone was significantly decreased in PCOS group. (civilica.com)
  • Integrins are a family of heterodimeric transmembrane glycoproteins involved in a wide range of cell-to-extracellular matrix (ECM) and cell-to-cell interactions. (thno.org)
  • Those integrins that don't carry this inserted domain also have an A-domain in their ligand binding site, but this A-domain is found on the β subunit. (wikipedia.org)
  • The X-ray crystal structure obtained for the complete extracellular region of one integrin, αvβ3, shows the molecule to be folded into an inverted V-shape that potentially brings the ligand-binding sites close to the cell membrane. (wikipedia.org)
  • Approved by the FDA in 2014, vedolizumab works differently: it blocks α4β7 integrin. (medscape.com)
  • Bass, MD. / SHARPINing integrin inhibition . (bris.ac.uk)
  • These were labeled with gallium-68 and screened regarding their suitability for in-vivo imaging of αvβ6 integrin expression by positron emission tomography (PET) and ex-vivo biodistribution in SCID mice bearing H2009 tumor (human lung adenocarcinoma) xenografts. (cipsm.de)
  • For these, αvβ6 integrin expression in tumor and other tissues was determined by β6 immunohistochemistry (IHC). (cipsm.de)
  • Despite the multimers showed higher αvβ6 integrin affinities (23-120 pM) than the monomers (260 pM), best results, that is, low background uptake and excellent tumor delineation, were obtained with the TRAP-based monomer 68 Ga-Avebehexin. (cipsm.de)
  • Thus, β 3 integrin has the potential to control processes such as tumor growth and wound healing by regulating BMDC recruitment to sites undergoing pathological and adaptive angiogenesis. (rupress.org)
  • Integrins carrying this domain either bind to collagens (e.g. integrins α1 β1, and α2 β1), or act as cell-cell adhesion molecules (integrins of the β2 family). (wikipedia.org)
  • A key adhesion molecule expressed by these cells is the α v β 3 integrin. (rupress.org)
  • We demonstrate that α v β 3 integrin enhances BMDC recruitment and retention at angiogenic sites by mediating cellular adhesion and transmigration of BMDCs through the endothelial monolayer but not their release from the bone niche. (rupress.org)
  • Integrin alpha 3/CD49c " has 17 results in Products. (rndsystems.com)
  • Detects human Integrin alpha 2/CD49b. (novusbio.com)
  • The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). (novusbio.com)
  • Staining of M21 cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (Product # 13-4714-85) (open histogram) or 0.5 µg of Anti-Human CD51/CD61 (Integrin alpha/beta 3) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). (thermofisher.com)
  • CD51 and CD61 molecules (alpha V and beta 3 integrins) form a complex. (thermofisher.com)
  • Actin reorganization is thought to be dependent on alpha (v)beta (3/5) integrin stimulation. (cnrs.fr)
  • The capacity of F1 to mediate Rap1-dependent integrin activation depends on a unique loop in F1 that transforms into an amphipathic helix upon binding to membrane lipids. (biorxiv.org)
  • The presence of integrins allows rapid and flexible responses to events at the cell surface (e.g. signal platelets to initiate an interaction with coagulation factors). (wikipedia.org)
  • however, this interaction makes a negligible contribution to integrin activation in CHO cells or platelets. (biorxiv.org)
  • Thus, a novel Rap1 binding site and a lipid-dependent amphipathic helix in talin1 F1 cooperate to enable a direct Rap1-talin1 interaction to cause integrin activation. (biorxiv.org)
  • Furthermore, αvβ6 integrin plays an important role for the epithelial-mesenchymal interaction and the development of fibrosis. (cipsm.de)
  • Integrin beta-4 signaling plays a key role in mouse embryogenesis. (pacificfertility.ca)
  • Reacts with human Integrin Beta 5. (covalab.com)
  • Among the known carbapenemases are Klebsiella pneumoniae carbapenemase, or KPC, and Verona integrin-encoded metallo-Beta-lactamase, or VIM. (cdc.gov)
  • Interestingly, the addition of exogenous dRP to human platelets significantly increased platelet aggregation and integrin αIIbβ3 activation in response to thrombin. (bath.ac.uk)
  • In cholangiocytes, CCN1 activated NF-κB through integrin α v β 5 /α v β 3 , leading to Jag1 expression, JAG1/NOTCH signaling, and cholangiocyte proliferation. (jci.org)
  • Because of highly sensitive PET imaging even of tissues with low αvβ6 integrin expression density, we anticipate clinical applicability of 68 Ga-Avebehexin for imaging of αvβ6 tumors and fibrosis by PET. (cipsm.de)
  • Human and mouse platelets responded to dRP treatment with a sizeable increase in reactive oxygen species (ROS) generation and the pre-treament with the antioxidant apocynin abolished the effect of dRP on aggregation and integrin activation. (bath.ac.uk)
  • Integrins clearly play a key role in regulating both mitogenic signalling and cell migration. (nih.gov)
  • Integrins and PAKs also play a role in the control of cell migration. (nih.gov)
  • These studies emphasize the intricate connection between integrins and Rho-family GTPases and their effectors in controlling both mitogenesis and migration. (nih.gov)
  • Using knockin mice expressing functionally stunted β 3 integrin, we show that bone marrow transplantation rescues impaired angiogenesis in these mice by normalizing BMDC recruitment. (rupress.org)
  • In light of these findings, precisely how β1 integrin transduces ECM cues via signaling intermediates, and the mechanism by which this translates to cellular responses, need to be addressed. (jneurosci.org)
  • Major conclusions: The observed mild phenotypes of mice deficient in collagen-binding integrins and the relatively restricted availability of integrin-binding sequences in mature fibrillar collagen matrices support the existence of indirect collagen-binding mechanisms in parallel with direct collagen binding in vivo. (uib.no)
  • Blockade of integrin α v β 5 /α v β 3 , NF-κB, or NOTCH signaling in WT mice also resulted in defective ductular reaction after BDL. (jci.org)
  • The MSDS of Anti-human for Integrin is available from Karlan upon request. (dnamethsoc.com)
  • Human integrin α10β1-selected adipose tissue-derived MSCs (integrin α10β1-MSCs) have shown immunomodulatory and regenerative effects in various disease models. (lu.se)