For example, LFA1 (CD11a/CD18) short representation of Lymphocyte Function-associated Antigen 1, also called αLβ2 integrin Mac1 (CD11b/CD18) present on macrophages that is also called Macrophage-1 antigen (CR3) and αMβ2 integrin. (wikipedia.org)
CD18 non-covalently associates with CD11a, CD11b, or CD11c. (biolegend.com)
CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
It forms αβ heterodimers with the CD11 alpha subunits ITGAL (αL aka CD11a forming integrin LFA-1), ITGAM (αM aka CD11b, forming integrin Mac-1) and ITGAX (αX aka CD11c, forming integrin p150/95). (guidetopharmacology.org)
In particular, CD11 is composed of CD11a, CD11b and CD11c. (thermofisher.com)
The C71/16 monoclonal antibody specifically binds to mouse CD18 which is also known as the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αxβ2 integrin). (bdbiosciences.com)
Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c). (bdbiosciences.com)
The beta-2 (B2) integrin (leukocyte integrin, CD11/CD18) subfamily comprising three members, each consisting of a shared beta subunit (CD18) non-covalently associated with unique alpha subunits (CD11a, CD11b, CD11c). (medicaljournals.se)
Background: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). (icbinhibitor.com)
CD112
In cell biology, CD11 is the α (alpha) component of various integrins, especially ones in which the β (beta) component is CD18 (β2) and mediate leukocyte adhesion. (wikipedia.org)
1998) Hu23F2G, an antibody recognizing the leukocyte CD11/CD18 integrin, reduces injury in a rabbit model of transient focal cerebral ischemia. (guidetopharmacology.org)
Subunit5
CD18, also known as integrin β2 subunit, LFA-1 β subunit, and β2 integrin, is a 90 - 95 kD type I glycoprotein. (biolegend.com)
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
The CD49b protein is an integrin alpha subunit. (wikidoc.org)
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. (biolegend.com)
An integrin alpha subunit that is unique in that it does not contain an I-domain, and its proteolytic cleavage site is near the middle of the extracellular portion of the polypeptide rather than close to the membrane, as in other integrin alpha subunits. (musc.edu)
Antibody6
Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
The following product was used in this experiment: CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscience™ from Thermo Fisher Scientific, catalog # 25-0114-82, RRID AB_469590. (thermofisher.com)
Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. (thermofisher.com)
The Langerhans' cells (LC) were identified with a monoclonal antibody to OKT6 antigen, by employing an immunofluorescence technique. (medicaljournals.se)
Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 ( MCA551 ) High power. (bio-rad-antibodies.com)
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. (thermofisher.com)
Receptor3
CD11c/CD18 also called complement receptor 4 (CR4) and αXβ2 integrin. (wikipedia.org)
CR2/CD21 interacts with C3b degradation products C3dg and C3d and can act synergistically with the B-cell antigen receptor (BCR) in B-cell activation. (medscape.com)
CD11c non-covalently associates with integrin β 2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. (biolegend.com)
It is now recognized that several non-DC myeloid populations express CD11c, including alveolar macrophages and resident (Ly6C low) monocytes and that some macrophage populations express both CD11c and MHC class II [8, 9]. (studybuff.com)
M1 macrophages were identified as F4/80-positive/CD11c-positive/CD206-negative and M2 macrophages were identified as F4/80-positive/CD11c-negative/CD206-positive. (studybuff.com)
CD11c has been proposed to function in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T cell proliferation by Langerhans cells. (studybuff.com)
Cluster of differentiation 14 (CD14) was described as monocyte/ macrophage differentiation antigen on the surface of myeloid lineage, such as monocytes, macrophages and dendritic cells (DCs). (studybuff.com)
CD11c is a type I transmembrane protein that is expressed on monocytes, granulocytes, a subset of B cells, dendritic cells, and macrophages and may be demonstrated on B-cell chronic lymphocytic leukemia, marginal zone lymphomas, and hairy cell leukemia. (studybuff.com)
CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. (biolegend.com)
Leukocyte6
Beta2-integrins are essential for leukocyte extravasation to sites of infection ( i.e. leukocyte trafficking), and other immunological processes including neutrophil phagocytosis and ROS production, and T cell activation. (guidetopharmacology.org)
2019) Beta2-Integrins and Interacting Proteins in Leukocyte Trafficking, Immune Suppression, and Immunodeficiency Disease. (guidetopharmacology.org)
CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. (thermofisher.com)
CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. (biolegend.com)
Molecules2
Integrins are cell surface molecules of importance in a wide variety of cellular functions, including morphogenesis, cell migration and cell matrix interactions. (medicaljournals.se)
Tumor immunogenicity results from the processing, and subsequent presentation of tumor antigens released from dead or dying tumor cells on MHC molecules expressed on antigen-presenting cells (APC). (aacrjournals.org)
Subunits1
In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. (icbinhibitor.com)
Functional studies revealed increased expression of ICAM-1 on induced patient-derived endothelial cells (iECs) and of β2-integrins on patient neutrophils that increase neutrophil adhesion and vascular transendothelial migration (TEM). (nature.com)
Macrophage3
There was no consistent decrease in the migration of CD11b + F4/80 + (macrophage) or SSC hi GR-1 + (neutrophil) populations. (biomedcentral.com)
Osteopontin mediates macrophage chemotaxis via a4 and a9 integrins and survival via the a4 integrin. (musc.edu)
Granulocytes1
Human peripheral blood granulocytes were stained with CD11c (clone S-HCL-3) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram). (biolegend.com)
Protein2
Multiple inherited mutations in the integrin β2 protein (CD18) that reduce expression or function have been identified as the causative factor in patients with LAD1. (guidetopharmacology.org)
Integrin alpha-2 or CD49b (cluster of differentiation 49b) is a protein which in humans is encoded by the CD49b gene . (wikidoc.org)
Myeloid2
Similarly, CD11b has often been used as a marker for all myeloid cell types. (studybuff.com)
CD11b is considered a pan-myeloid marker (expressed after granulocyte-monocyte progenitors (GMP) phase in the bone marrow). (studybuff.com)
They can be identified using flow cytometry or immunohistochemical staining by their specific expression of proteins such as CD14, CD40, CD11b, CD64, F4/80 (mice)/EMR1 (human), lysozyme M, MAC-1/MAC-3 and CD68. (studybuff.com)
Alexa Fluor4
Figure B. FITC conjugated Mouse anti Human CD120a ( MCA1340F ) and Alexa Fluor 647 conjugated Mouse anti Human CD11b ( MCA551A647 ). (bio-rad-antibodies.com)
Figure B. RPE conjugated Mouse anti Human CD14 ( MCA1568PE ) and Alexa Fluor 647 conjugated Mouse anti Human CD11b ( MCA551A647 ). (bio-rad-antibodies.com)
Then the section was stained with 5 µg/ml of CD11b (clone M1/70) Alexa Fluor® 594 (red), 2.5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective. (biolegend.com)
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 594 (filled histogram). (biolegend.com)
Tumor-specific2
Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques. (wikidoc.org)
LN metastases resist T cell-mediated cytotoxicity, induce antigen-specific regulatory T cells, and generate tumor-specific immune tolerance that subsequently facilitates distant tumor colonization. (stanford.edu)
Ly6C5
In mice, MDSCs are Mac-1 + (CD11b) and Gr1 + (Ly6G/Ly6C) and are subcategorized based on the differential expression of Ly6G and Ly6C. (netlify.app)
Granulocytic MDSCs are defined as CD11b + Ly6G + Ly6C low, whereas monocytic MDSCs are defined as CD11b + Ly6G low/− Ly6C high. (netlify.app)
G-MDSC (Ly6CloLy6G+) 5 0 * * CD11b * + cells per lung (10 6) 6 5 4 3 2 1 0 * Veh Bleo PBS CL 2 MDP PBS CL 2 CD11b MDP CD11c Ly6G Ly6C CXCR2 Ly6C Ly6G CL 2MDP Jan 29, 2018 MDSC development and cell subsets in mice and humans. (netlify.app)
In mice, G-MDSCs are defined as CD11b+Ly6ClowLy6G+ cells, and M-MDSCs We assayed Ly6G and Ly6C expression in different MDSC subsets obtained from Gfi1:GFP knock-in mice with sepsis following CLP challenge. (netlify.app)
Although both mice and human MDSCs express CD11b, human MDSCs do not have the Gr-1 antigen (Ly6G/Ly6C) and are more complex. (netlify.app)
Migration1
pDCs express lower levels of CD11c than cDCs, and cDCs downregulate CD11c upon migration or activation. (studybuff.com)
Cells11
ATMs comprised CD11c + CD206 + cells in "crown" aggregates and solitary CD11c − CD206 + cells at adipocyte junctions. (diabetesjournals.org)
Tissue culture medium conditioned by CD11c + ATMs, but not CD11c − ATMs or other stromovascular cells, impaired insulin-stimulated glucose uptake by human adipocytes. (diabetesjournals.org)
Overall, CD11c was expressed in all B cell subpopulations, suggesting a role in all B-cell development steps, with an increased frequency in antigen-driven B cells, which accumulate with age of HD, independently of gender. (studybuff.com)
First discovered by Ralph Steinman and Zanvil A. Cohn in the 1970s, named for their tree-like branches or "dendrites", these cells patrol our bodies, ingest pathogens, process them, and present fragments (antigens) to T-cells, thus initiating a targeted immune response [2]. (origene.com)
cDC1: These cells are very effective at presenting antigens to CD8+ T cells, a type of cytotoxic T cell that can kill infected or cancerous cells. (origene.com)
cDC2: These cells are specialized in presenting antigens to CD4+ T cells, a type of helper T cell that helps coordinate the immune response. (origene.com)
We isolated fecal content and small intestinal leukocytes from murine, Roux-en-Y and end-to-end anastomoses, to identify microbiome changes and subsequent alterations in the regulatory and pro-inflammatory immune cells 3 days post-operative. (biomedcentral.com)
Homeostatic Raldh expression in immune cells is well described in gut-associated lymphoid tissues (GALT) [7] - [11] , where RA synthesis by antigen presenting cells (APCs) contributes to the recruitment and function of local lymphocyte populations. (prolekarniky.cz)
Hypoxia stimulates hypoxia inducible factor (HIF)-dependent expression of CXCL12 and KITL promoting mobilization from the bone marrow and recruitment to primary tumor and metastatic sites of CXCR4 + CD11b + bone marrow-derived cells and KITbCD11b + cells assisting vasculogenesis and metastasis respectively (Kuonen et al. (biomedcentral.com)
Recruitment of CD11b + CD11c + myelomonocytic cells to the metastatic site was also found after whole thorax irradiation at a dose of 15 Gy of mice that significantly enhanced seeding and metastatic growth of intravenously injected cancer cells. (biomedcentral.com)
Glycoproteins1
Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. (wikidoc.org)
FITC1
Figure B. FITC conjugated Mouse anti Human CD31 ( MCA1738F ) and RPE conjugated Mouse anti Human CD11b ( MCA551PE ). (bio-rad-antibodies.com)
Adhesion4
In vitro studies show that Leishmania infection decreases the adhesion of inflammatory phagocytes to connective tissue by a mechanism dependent on the modulation of integrin function. (biomedcentral.com)
CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b. (biolegend.com)
Using assays to quantify CD11c-mediated cell adhesion, we demonstrate that CD11c recognizes ICAM-2 and VCAM-1. (studybuff.com)
Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling. (wikidoc.org)
Expression1
Hammarstrom S. The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues. (wikidoc.org)
Enzymes1
In addition, CD11c + ATMs were enriched for mitochondria and for RNA transcripts encoding mitochondrial, proteasomal, and lysosomal proteins, fatty acid metabolism enzymes, and T-cell chemoattractants, whereas CD11c − ATMs were enriched for transcripts involved in tissue maintenance and repair. (diabetesjournals.org)
Immune2
In addition, the machinery of CD11c + ATMs indicates they metabolize lipid and may initiate adaptive immune responses. (diabetesjournals.org)
They play an essential role in the induction of adaptive immune response against infectious agents and in generating tolerance against self-antigens [3]. (origene.com)
Tissue1
In obese women, CD11c + ATM density was greater in subcutaneous than omental adipose tissue and correlated with markers of insulin resistance. (diabetesjournals.org)
These findings identify proinflammatory CD11c + ATMs as markers of insulin resistance in human obesity. (diabetesjournals.org)
Here, using these markers and functional assays, we aimed to identify whether MDSC are induced during MDSC (CD11b+Gr-1highF4/80-) from MO-MDSC (CD11b+Gr-1intF4/80int). (netlify.app)
Starting with 4T1 tumor-bearing BALB/c mouse splenocytes, the MDSC content (CD11b+Gr1+) of the isolated fraction is typically 94.3 ± 2.1% (mean ± SD using the purple EasySep™ Magnet). (netlify.app)