The C71/16 monoclonal antibody specifically binds to mouse CD18 which is also known as the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αxβ2 integrin). (bdbiosciences.com)
Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c). (bdbiosciences.com)
and the beta subunit, unique to leukocytes, is beta-2 or CD18. (wikipedia.org)
The beta-2 (B2) integrin (leukocyte integrin, CD11/CD18) subfamily comprising three members, each consisting of a shared beta subunit (CD18) non-covalently associated with unique alpha subunits (CD11a, CD11b, CD11c). (medicaljournals.se)
Background: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). (icbinhibitor.com)
Background: It recognizes a transmembrane glycoprotein of 95kDa, identified as CD18 or integrin -2 (Workshop III). (m-entrepreneurship.com)
The CD11/CD18 complex is part of the beta-2 integrin family and is important in adhesion and phagocytosis (see Table 1). (medscape.com)
CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. (newmarketscientific.com)
CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component. (newmarketscientific.com)
Cats affected with LAD1 suffer from a deficiency of the β subunit of CD18. (basepaws.com)
The deficiency of leukocyte integrin CD18-β subunit is caused by an autosomal recessive mutation in the integrin β-2 subunit ( ITBG2 ) gene. (basepaws.com)
This subunit of the CD18 protein forms a heterodimer (a complex composed of two different proteins) with one of 4 different α-subunits of the CD11 protein. (basepaws.com)
Together, CD11 and CD18 form CD11/CD18 integrins, which are expressed on all leukocytes' surface membranes. (basepaws.com)
Valmu L , Autero M, Siljander P, Patarroyo M and Gahmberg C G (1991) Phosphorylation of the ß-subunit of CD11/CD18 in-tegrins by protein kinase C correlates with leukocyte adhesion. (leenavalmu.fi)
Li R, Nortamo P, Valmu L , Tolvanen M, Huuskonen J, Kantor C and Gahmberg C G (1993) A peptide from ICAM-2 binds to the Leu-kocyte Integrin CD11a/CD18 and inhibits endot-helial cell adhesion. (leenavalmu.fi)
Valmu L and Gahmberg C G (1995) Treatment with okadaic acid reveals strong threonine phosphorylation of CD18 after activation of CD11/CD18 leukocyte integrins with phorbol esters or CD3 antibodies. (leenavalmu.fi)
Valmu L , Hilden T J, van Willigen G and Gahmberg C G (1999) Characterization of b2 (CD18) integrin phosphorylation in phorbol ester activated T lymphocytes. (leenavalmu.fi)
Valmu L , Fagerholm S, Suila H and Gahmberg C G (1999) The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester activated cells correlates with CD18 phosphorylation. (leenavalmu.fi)
Leukocyte adhesion deficiency type I (LAD I) is a failure to express CD18, which composes the common ß 2 subunit of LFA1 family (ß2 integrins). (medscape.com)
Heterodimer3
We have used monoclonal antibodies (mAbs) as probes to study the dependence of the structure of different regions of the alphaM subunit on association with the beta2 subunit in the alphaM/beta2 heterodimer. (embl.de)
Each integrin heterodimer consists of an alpha (α) and a beta (β) subunit associated by noncovalent interactions forming an extracellular ligand-binding head, two multi-domain `legs', two single-pas s transmembrane helices and two short cytoplasmic tails. (mechanobio.info)
Protein3
These experiments yielded the protein name "integrin" as a description of the proteins' integral role in cellular adhesion processes and the transmembrane association between the extracellular matrix and the cytoskeleton. (wikipedia.org)
Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. (affbiotech.com)
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). (affbiotech.com)
Glycoprotein2
LFA-1 is a heterodimeric glycoprotein with non-covalently linked subunits. (wikipedia.org)
The rabies virus envelope glycoprotein (RABV G) is a major rabies virus antigen and contains neutralizing epitopes, which are primary candidates for subunit vaccines and diagnostic antigens. (bvsalud.org)
Antigen1
Lymphocyte function-associated antigen 1 (LFA-1) is an integrin found on lymphocytes and other leukocytes. (wikipedia.org)
AlphaM subunit5
The structure of the beta-propeller domain and C-terminal region of the integrin alphaM subunit. (embl.de)
The alphaM subunit of integrin Mac-1 contains several distinct regions in its extracellular segment. (embl.de)
All of the mAbs to the I-domain immunoprecipitated the unassociated alphaM precursor and reacted with the alphaM subunit expressed alone on the surface of COS cells. (embl.de)
By contrast, four mAbs to the beta-propeller domain did not react with the unassociated alphaM precursor nor with the uncomplexed alphaM subunit expressed on COS cell surface. (embl.de)
A subset of four mAbs that reacted with the alphaM/beta2 complex but not with the unassociated alphaM subunit were mapped to one subregion, residues 718-759, and five other mAbs that recognized both the unassociated and the complexed alphaM subunit were localized to three other subregions, residues 599-679, 820-882, and 943-1047. (embl.de)
Heterodimers1
The integrin family of proteins consists of alpha and beta subtypes, which form transmembrane heterodimers. (mechanobio.info)
Lymphocytes1
Notably, transfection of the subjects' lymphocytes with KINDLIN3 complementary DNA but not CALDAGGEF1 cDNA reverses the LAD-III defect, restoring integrin-mediated adhesion and migration. (ox.ac.uk)
Small subunit1
It is the small subunit of the MHC class I molecule. (lookformedical.com)
Leukocyte adhesion2
Leukocyte adhesion deficiency-III is caused by mutations in KINDLIN3 affecting integrin activation. (ox.ac.uk)
Subjects with leukocyte adhesion deficiency-1 (LAD-I) do not express beta2 integrins because of mutations in the gene specifying the beta2 subunit, and they suffer recurrent bacterial infections. (ox.ac.uk)
Adhesion receptors2
Integrins are the major adhesion receptors of leukocytes and platelets. (ox.ac.uk)
Integrins function as adhesion receptors for extracellular ligands and transduce biochemical signals into the cell, through downstream effector proteins. (mechanobio.info)
Cysteine-rich1
The β subunit comprises of 4 cysteine-rich epidermal growth factor (EGF) repeats, a hybrid domain (split in sequence), an I-like domain (βI) and a plexin-sempahorin-integrin (PSI) domain. (mechanobio.info)
Fibrinogen4
Integrins α-m/β-2 and α-x/β-2 are receptors for the IC3B fragment of the third complement component and for fibrinogen. (lu.se)
Integrin α-x/β 2 recognizes the sequence g-p-r in fibrinogen α-chain. (lu.se)
Beta1 and beta2 integrin function on leukocytes is crucial for a successful immune response and the platelet integrin alpha(IIb)beta3 initiates the process of blood clotting through binding fibrinogen. (ox.ac.uk)
Beta17
LFA-1 has two subunits designated as the alpha subunit and beta subunit. (wikipedia.org)
Rap1 assists in breaking the constraint between the alpha and beta subunits of LFA-1. (wikipedia.org)
The activation complex further destabilizes the alpha and beta subunits. (wikipedia.org)
Chemokines also stimulate an I-like domain on the beta subunit, which causes the MIDAS site on the beta subunit to bind to glutamate on the I domain of the alpha subunit. (wikipedia.org)
This binding process causes the beta subunit to pull down the alpha 7 helix of the I domain, exposing and opening up the MIDAS site on the alpha subunit for binding. (wikipedia.org)
LAD1, a subtype of LAD, is caused by a lack of integrins that contain the beta subunit, including LFA-1. (wikipedia.org)
They contain both alpha and beta subunits. (embl.de)
Alpha integrins are proposed to contain a domain containing a 7-fold repeat that adopts a beta-propellor fold. (embl.de)
Dependence on beta subunit association and prediction of domains. (embl.de)
The four mAbs were mapped to three subregions in three different beta-sheets, making it unlikely that each recognized an interface between the alpha and beta subunits. (embl.de)
These results suggest that folding of different beta-propeller subregions is coordinate and is dependent on association with the beta2 subunit. (embl.de)
This suggests that much of the region C-terminal to the beta-propeller domain folds independently of association with the beta2 subunit. (embl.de)
Our data provide new insights into how different domains in the integrin alpha and beta subunits may interact. (embl.de)
Integrators of epidermal growth and differentiation: distinct functions for beta 1 and beta 4 integrins. (embl.de)
Integrins are cell surface molecules of importance in a wide variety of cellular functions, including morphogenesis, cell migration and cell matrix interactions. (medicaljournals.se)
Ligand1
The α subunit leg consists of a thigh and 2 calf domains that support the ligand binding head formed by a β-propeller domain with 7 repeats forming the blades (shown as a cylinder in the figure below). (mechanobio.info)
Cytoskeleton1
Integrins are proteins that function mechanically, by attaching the cell cytoskeleton to the extracellular matrix (ECM), and biochemically, by sensing whether adhesion has occurred. (mechanobio.info)
Proteins1
The proteins exist as large oligomers that are formed from ALPHA-CRYSTALLIN A CHAIN and ALPHA-CRYSTALLIN B CHAIN subunits. (lookformedical.com)
Expression2
Mitochondrial complexes are multi-subunits enzymes formed of up to ≈ 50 subunits (complexe I). Complexes expression and or assembly are commobnly analyzed using Blue-Native electrophoresis. (univ-angers.fr)
However, before we can gain a proper understanding of their role in mice that are missing these ion channel subunits, we need to know their developmental expression profile in the developing mouse. (edu.au)
Deficiency1
Their hematopoietically-derived cells express beta1, beta2 and beta3 integrins, but defective inside-out signaling causes immune deficiency and bleeding problems. (ox.ac.uk)
Migration1
These integrins are essential for proper leukocyte migration, mediating intercellular contacts. (icbinhibitor.com)
Bind1
Integrins on circulating cells bind poorly to their ligands but become active after 'inside-out' signaling through other membrane receptors. (ox.ac.uk)
The general binding region of the alpha subunit is the I-domain. (wikipedia.org)
Integrin α subunit domains: Top: Linear domain arrangement. (mechanobio.info)
The αI domain is present in some subtypes of the α subunit. (mechanobio.info)
Human2
Hallikas O K, Aaltonen J M, von Koskull H, Lindberg L A, Valmu L , Kalkkinen N, Wahlström T, Kataoka H, Andersson L, Lindholm D and Schröder J (2006) Identification of antibodies against HAI-1 and integrin α6β4 as immunohistochemical markers of human villous cytotrophoblast. (leenavalmu.fi)
Valmu L , Alfthan H, Hotakainen K, Birken S and Stenman U-H (2006) Site-specific glycan analysis on human chorionic ganadotropin β-subunit from malignancy and pregnancy by liquid chromatography-electrospray mass spectrometry. (leenavalmu.fi)
Mice1
LFA-1, a leukocyte integrin, was first discovered by Timothy Springer in mice in the 1980s. (wikipedia.org)